Effect of hot air, UV-C, white light and modified atmosphere treatments on expression of chlorophyll degrading genes in postharvest broccoli (Brassica oleracea L.) florets

Autores
Büchert, Agustín Matias; Civello, Pedro Marcos; Martinez, Gustavo Adolfo
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Several treatments were applied in order to delay postharvest degreening in broccoli florets and investi-gate their effects on genes associated with chlorophyll catabolism. Degradation of chlorophylls is the most evident visual manifestation of broccoli postharvest deterioration, occurring rapidly due to the immature stage in which the material is harvested. Treatments such as storage in modified atmosphere, exposure to hot air, UV-C and white lamps were employed in the current work to induce a delay in degreening and chlorophyll degradation. Expression of genes possibly related to chlorophyll catabolism was analyzed in these samples and discussed. Chlorophyllases, the enzymes traditionally believed to remove the phytol side chain from chlorophyll appear to have a gene expression that was not regulated by postharvest treatments. Pheophytinase, a recently discovered new enzyme in this metabolic pathway, correlated chlorophyll loss accurately in heat, UV-C and white-light treatments, but not in modified atmospheres. Results presented in this work indicate that postharvest treatments that delay chlorophyll degradation have a higher effect on the expression of pheophytinase rather than on chlorophyllase genes.
Fil: Büchert, Agustín Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Civello, Pedro Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Martinez, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Materia
CHLOROPHYLLASE
HEAT TREATMENT
MODIFIED ATMOSPHERE
PHEOPHYTINASE
SENESCENCE
UV-C
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/198523
Acceder
id CONICETDig_421c785db95ffbbb2a7ea3c23f1623b1
oai_identifier_str oai:ri.conicet.gov.ar:11336/198523
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of hot air, UV-C, white light and modified atmosphere treatments on expression of chlorophyll degrading genes in postharvest broccoli (Brassica oleracea L.) floretsBüchert, Agustín MatiasCivello, Pedro MarcosMartinez, Gustavo AdolfoCHLOROPHYLLASEHEAT TREATMENTMODIFIED ATMOSPHEREPHEOPHYTINASESENESCENCEUV-Chttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Several treatments were applied in order to delay postharvest degreening in broccoli florets and investi-gate their effects on genes associated with chlorophyll catabolism. Degradation of chlorophylls is the most evident visual manifestation of broccoli postharvest deterioration, occurring rapidly due to the immature stage in which the material is harvested. Treatments such as storage in modified atmosphere, exposure to hot air, UV-C and white lamps were employed in the current work to induce a delay in degreening and chlorophyll degradation. Expression of genes possibly related to chlorophyll catabolism was analyzed in these samples and discussed. Chlorophyllases, the enzymes traditionally believed to remove the phytol side chain from chlorophyll appear to have a gene expression that was not regulated by postharvest treatments. Pheophytinase, a recently discovered new enzyme in this metabolic pathway, correlated chlorophyll loss accurately in heat, UV-C and white-light treatments, but not in modified atmospheres. Results presented in this work indicate that postharvest treatments that delay chlorophyll degradation have a higher effect on the expression of pheophytinase rather than on chlorophyllase genes.Fil: Büchert, Agustín Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Civello, Pedro Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Martinez, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaElsevier Science2011-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/198523Büchert, Agustín Matias; Civello, Pedro Marcos; Martinez, Gustavo Adolfo; Effect of hot air, UV-C, white light and modified atmosphere treatments on expression of chlorophyll degrading genes in postharvest broccoli (Brassica oleracea L.) florets; Elsevier Science; Scientia Horticulturae; 127; 3; 1-2011; 214-2190304-4238CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0304423810004930info:eu-repo/semantics/altIdentifier/doi/10.1016/j.scienta.2010.11.001info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:03Zoai:ri.conicet.gov.ar:11336/198523instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:04.096CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of hot air, UV-C, white light and modified atmosphere treatments on expression of chlorophyll degrading genes in postharvest broccoli (Brassica oleracea L.) florets
title Effect of hot air, UV-C, white light and modified atmosphere treatments on expression of chlorophyll degrading genes in postharvest broccoli (Brassica oleracea L.) florets
spellingShingle Effect of hot air, UV-C, white light and modified atmosphere treatments on expression of chlorophyll degrading genes in postharvest broccoli (Brassica oleracea L.) florets
Büchert, Agustín Matias
CHLOROPHYLLASE
HEAT TREATMENT
MODIFIED ATMOSPHERE
PHEOPHYTINASE
SENESCENCE
UV-C
title_short Effect of hot air, UV-C, white light and modified atmosphere treatments on expression of chlorophyll degrading genes in postharvest broccoli (Brassica oleracea L.) florets
title_full Effect of hot air, UV-C, white light and modified atmosphere treatments on expression of chlorophyll degrading genes in postharvest broccoli (Brassica oleracea L.) florets
title_fullStr Effect of hot air, UV-C, white light and modified atmosphere treatments on expression of chlorophyll degrading genes in postharvest broccoli (Brassica oleracea L.) florets
title_full_unstemmed Effect of hot air, UV-C, white light and modified atmosphere treatments on expression of chlorophyll degrading genes in postharvest broccoli (Brassica oleracea L.) florets
title_sort Effect of hot air, UV-C, white light and modified atmosphere treatments on expression of chlorophyll degrading genes in postharvest broccoli (Brassica oleracea L.) florets
dc.creator.none.fl_str_mv Büchert, Agustín Matias
Civello, Pedro Marcos
Martinez, Gustavo Adolfo
author Büchert, Agustín Matias
author_facet Büchert, Agustín Matias
Civello, Pedro Marcos
Martinez, Gustavo Adolfo
author_role author
author2 Civello, Pedro Marcos
Martinez, Gustavo Adolfo
author2_role author
author
dc.subject.none.fl_str_mv CHLOROPHYLLASE
HEAT TREATMENT
MODIFIED ATMOSPHERE
PHEOPHYTINASE
SENESCENCE
UV-C
topic CHLOROPHYLLASE
HEAT TREATMENT
MODIFIED ATMOSPHERE
PHEOPHYTINASE
SENESCENCE
UV-C
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Several treatments were applied in order to delay postharvest degreening in broccoli florets and investi-gate their effects on genes associated with chlorophyll catabolism. Degradation of chlorophylls is the most evident visual manifestation of broccoli postharvest deterioration, occurring rapidly due to the immature stage in which the material is harvested. Treatments such as storage in modified atmosphere, exposure to hot air, UV-C and white lamps were employed in the current work to induce a delay in degreening and chlorophyll degradation. Expression of genes possibly related to chlorophyll catabolism was analyzed in these samples and discussed. Chlorophyllases, the enzymes traditionally believed to remove the phytol side chain from chlorophyll appear to have a gene expression that was not regulated by postharvest treatments. Pheophytinase, a recently discovered new enzyme in this metabolic pathway, correlated chlorophyll loss accurately in heat, UV-C and white-light treatments, but not in modified atmospheres. Results presented in this work indicate that postharvest treatments that delay chlorophyll degradation have a higher effect on the expression of pheophytinase rather than on chlorophyllase genes.
Fil: Büchert, Agustín Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Civello, Pedro Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Martinez, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
description Several treatments were applied in order to delay postharvest degreening in broccoli florets and investi-gate their effects on genes associated with chlorophyll catabolism. Degradation of chlorophylls is the most evident visual manifestation of broccoli postharvest deterioration, occurring rapidly due to the immature stage in which the material is harvested. Treatments such as storage in modified atmosphere, exposure to hot air, UV-C and white lamps were employed in the current work to induce a delay in degreening and chlorophyll degradation. Expression of genes possibly related to chlorophyll catabolism was analyzed in these samples and discussed. Chlorophyllases, the enzymes traditionally believed to remove the phytol side chain from chlorophyll appear to have a gene expression that was not regulated by postharvest treatments. Pheophytinase, a recently discovered new enzyme in this metabolic pathway, correlated chlorophyll loss accurately in heat, UV-C and white-light treatments, but not in modified atmospheres. Results presented in this work indicate that postharvest treatments that delay chlorophyll degradation have a higher effect on the expression of pheophytinase rather than on chlorophyllase genes.
publishDate 2011
dc.date.none.fl_str_mv 2011-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/198523
Büchert, Agustín Matias; Civello, Pedro Marcos; Martinez, Gustavo Adolfo; Effect of hot air, UV-C, white light and modified atmosphere treatments on expression of chlorophyll degrading genes in postharvest broccoli (Brassica oleracea L.) florets; Elsevier Science; Scientia Horticulturae; 127; 3; 1-2011; 214-219
0304-4238
CONICET Digital
CONICET
url http://hdl.handle.net/11336/198523
identifier_str_mv Büchert, Agustín Matias; Civello, Pedro Marcos; Martinez, Gustavo Adolfo; Effect of hot air, UV-C, white light and modified atmosphere treatments on expression of chlorophyll degrading genes in postharvest broccoli (Brassica oleracea L.) florets; Elsevier Science; Scientia Horticulturae; 127; 3; 1-2011; 214-219
0304-4238
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0304423810004930
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.scienta.2010.11.001
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842270143602753536
score 13.13397

Publicaciones similares