Antibacterial activity of a combination of cysteine and ciprofloxacin and its relationship with the generation of oxidative stress in extended-spectrum betalactamase-producing esch...

Autores
Martínez, S. R.; Bongiovanni, M. E.; Piersigilli, Andrea Laura; Albesa, Inés; Becerra, María Cecilia
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of the present work was to evaluate whether L-cysteine enhances theantibiotic susceptibility of extended-spectrum beta-lactamase (ESBL) Escherichia coli strains to ciprofloxacin and to identify the role of Reactive oxygen species (ROS) in the antimicrobial activity.The combined ef5fect of L-cysteine and ciprofloxacin was investigated by the macrodilution broth method and chemiluminescence assay. ESBL 1 and ESBL 2 strains presented a growth inhibition when they were incubated with 0.4 or 0.2 mM of cysteine, respectively compared to the control without antibiotic. Both strains at sub-inhibitory concentration of ciprofloxacin were combined withcysteine, and a clear growth inhibition respect to the control was observed. An increase of 68% in ROS occurred respect to the control when ESBL 1 was treated with a combination of ciprofloxacin and cysteine, while for ESBL 2 there was a rice of 127 %. The combination of ciprofloxacin with cysteine had the capacity to undergo redox cycling and ROS production with subsequent cellularinjury.
Fil: Martínez, S. R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Bongiovanni, M. E.. Hospital Privado Centro Medico de Córdoba; Argentina
Fil: Piersigilli, Andrea Laura. Sanatorio Aconcagua; Argentina
Fil: Albesa, Inés. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina
Fil: Becerra, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina
Materia
ANTIBIOTIC
L-CYSTEINE
RADICALS
ESCHERICHIA COLI
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/88137
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/88137
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antibacterial activity of a combination of cysteine and ciprofloxacin and its relationship with the generation of oxidative stress in extended-spectrum betalactamase-producing escherichia coli strainsMartínez, S. R.Bongiovanni, M. E.Piersigilli, Andrea LauraAlbesa, InésBecerra, María CeciliaANTIBIOTICL-CYSTEINERADICALSESCHERICHIA COLIhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The aim of the present work was to evaluate whether L-cysteine enhances theantibiotic susceptibility of extended-spectrum beta-lactamase (ESBL) Escherichia coli strains to ciprofloxacin and to identify the role of Reactive oxygen species (ROS) in the antimicrobial activity.The combined ef5fect of L-cysteine and ciprofloxacin was investigated by the macrodilution broth method and chemiluminescence assay. ESBL 1 and ESBL 2 strains presented a growth inhibition when they were incubated with 0.4 or 0.2 mM of cysteine, respectively compared to the control without antibiotic. Both strains at sub-inhibitory concentration of ciprofloxacin were combined withcysteine, and a clear growth inhibition respect to the control was observed. An increase of 68% in ROS occurred respect to the control when ESBL 1 was treated with a combination of ciprofloxacin and cysteine, while for ESBL 2 there was a rice of 127 %. The combination of ciprofloxacin with cysteine had the capacity to undergo redox cycling and ROS production with subsequent cellularinjury.Fil: Martínez, S. R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Bongiovanni, M. E.. Hospital Privado Centro Medico de Córdoba; ArgentinaFil: Piersigilli, Andrea Laura. Sanatorio Aconcagua; ArgentinaFil: Albesa, Inés. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; ArgentinaFil: Becerra, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; ArgentinaLife Science Informatics Publications2018-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/88137Martínez, S. R.; Bongiovanni, M. E.; Piersigilli, Andrea Laura; Albesa, Inés; Becerra, María Cecilia; Antibacterial activity of a combination of cysteine and ciprofloxacin and its relationship with the generation of oxidative stress in extended-spectrum betalactamase-producing escherichia coli strains; Life Science Informatics Publications; Research Journal of Life Science, Bioinformatics, Pharmaceutical and Chemical Sciences; 4; 3; 8-2018; 455-4632454.6348CONICET DigitalCONICETenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-26T08:55:23Zoai:ri.conicet.gov.ar:11336/88137instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-26 08:55:23.607CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antibacterial activity of a combination of cysteine and ciprofloxacin and its relationship with the generation of oxidative stress in extended-spectrum betalactamase-producing escherichia coli strains
title Antibacterial activity of a combination of cysteine and ciprofloxacin and its relationship with the generation of oxidative stress in extended-spectrum betalactamase-producing escherichia coli strains
spellingShingle Antibacterial activity of a combination of cysteine and ciprofloxacin and its relationship with the generation of oxidative stress in extended-spectrum betalactamase-producing escherichia coli strains
Martínez, S. R.
ANTIBIOTIC
L-CYSTEINE
RADICALS
ESCHERICHIA COLI
title_short Antibacterial activity of a combination of cysteine and ciprofloxacin and its relationship with the generation of oxidative stress in extended-spectrum betalactamase-producing escherichia coli strains
title_full Antibacterial activity of a combination of cysteine and ciprofloxacin and its relationship with the generation of oxidative stress in extended-spectrum betalactamase-producing escherichia coli strains
title_fullStr Antibacterial activity of a combination of cysteine and ciprofloxacin and its relationship with the generation of oxidative stress in extended-spectrum betalactamase-producing escherichia coli strains
title_full_unstemmed Antibacterial activity of a combination of cysteine and ciprofloxacin and its relationship with the generation of oxidative stress in extended-spectrum betalactamase-producing escherichia coli strains
title_sort Antibacterial activity of a combination of cysteine and ciprofloxacin and its relationship with the generation of oxidative stress in extended-spectrum betalactamase-producing escherichia coli strains
dc.creator.none.fl_str_mv Martínez, S. R.
Bongiovanni, M. E.
Piersigilli, Andrea Laura
Albesa, Inés
Becerra, María Cecilia
author Martínez, S. R.
author_facet Martínez, S. R.
Bongiovanni, M. E.
Piersigilli, Andrea Laura
Albesa, Inés
Becerra, María Cecilia
author_role author
author2 Bongiovanni, M. E.
Piersigilli, Andrea Laura
Albesa, Inés
Becerra, María Cecilia
author2_role author
author
author
author
dc.subject.none.fl_str_mv ANTIBIOTIC
L-CYSTEINE
RADICALS
ESCHERICHIA COLI
topic ANTIBIOTIC
L-CYSTEINE
RADICALS
ESCHERICHIA COLI
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of the present work was to evaluate whether L-cysteine enhances theantibiotic susceptibility of extended-spectrum beta-lactamase (ESBL) Escherichia coli strains to ciprofloxacin and to identify the role of Reactive oxygen species (ROS) in the antimicrobial activity.The combined ef5fect of L-cysteine and ciprofloxacin was investigated by the macrodilution broth method and chemiluminescence assay. ESBL 1 and ESBL 2 strains presented a growth inhibition when they were incubated with 0.4 or 0.2 mM of cysteine, respectively compared to the control without antibiotic. Both strains at sub-inhibitory concentration of ciprofloxacin were combined withcysteine, and a clear growth inhibition respect to the control was observed. An increase of 68% in ROS occurred respect to the control when ESBL 1 was treated with a combination of ciprofloxacin and cysteine, while for ESBL 2 there was a rice of 127 %. The combination of ciprofloxacin with cysteine had the capacity to undergo redox cycling and ROS production with subsequent cellularinjury.
Fil: Martínez, S. R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Bongiovanni, M. E.. Hospital Privado Centro Medico de Córdoba; Argentina
Fil: Piersigilli, Andrea Laura. Sanatorio Aconcagua; Argentina
Fil: Albesa, Inés. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina
Fil: Becerra, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina
description The aim of the present work was to evaluate whether L-cysteine enhances theantibiotic susceptibility of extended-spectrum beta-lactamase (ESBL) Escherichia coli strains to ciprofloxacin and to identify the role of Reactive oxygen species (ROS) in the antimicrobial activity.The combined ef5fect of L-cysteine and ciprofloxacin was investigated by the macrodilution broth method and chemiluminescence assay. ESBL 1 and ESBL 2 strains presented a growth inhibition when they were incubated with 0.4 or 0.2 mM of cysteine, respectively compared to the control without antibiotic. Both strains at sub-inhibitory concentration of ciprofloxacin were combined withcysteine, and a clear growth inhibition respect to the control was observed. An increase of 68% in ROS occurred respect to the control when ESBL 1 was treated with a combination of ciprofloxacin and cysteine, while for ESBL 2 there was a rice of 127 %. The combination of ciprofloxacin with cysteine had the capacity to undergo redox cycling and ROS production with subsequent cellularinjury.
publishDate 2018
dc.date.none.fl_str_mv 2018-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/88137
Martínez, S. R.; Bongiovanni, M. E.; Piersigilli, Andrea Laura; Albesa, Inés; Becerra, María Cecilia; Antibacterial activity of a combination of cysteine and ciprofloxacin and its relationship with the generation of oxidative stress in extended-spectrum betalactamase-producing escherichia coli strains; Life Science Informatics Publications; Research Journal of Life Science, Bioinformatics, Pharmaceutical and Chemical Sciences; 4; 3; 8-2018; 455-463
2454.6348
CONICET Digital
CONICET
url http://hdl.handle.net/11336/88137
identifier_str_mv Martínez, S. R.; Bongiovanni, M. E.; Piersigilli, Andrea Laura; Albesa, Inés; Becerra, María Cecilia; Antibacterial activity of a combination of cysteine and ciprofloxacin and its relationship with the generation of oxidative stress in extended-spectrum betalactamase-producing escherichia coli strains; Life Science Informatics Publications; Research Journal of Life Science, Bioinformatics, Pharmaceutical and Chemical Sciences; 4; 3; 8-2018; 455-463
2454.6348
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Life Science Informatics Publications
publisher.none.fl_str_mv Life Science Informatics Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1849873094079938560
score 13.011256

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