Pet and Stray Dogs as Reservoirs of Antimicrobial-Resistant Escherichia coli

Autores
Marchetti, María Laura; Buldain, Daniel Cornelio; Gortari Castillo, Lihuel; Buchamer, Andrea Verónica; Chirino Trejo, Manuel; Mestorino, Olga Nora
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The close contact between dogs and humans creates the best bridge for interspecies transmission of antimicrobial-resistant bacteria. The surveillance of its resistance including the detection of extended-spectrum beta-lactamases (ESBLs) in Escherichia coli as indicator bacteria is an important tool to control the use of antimicrobials. The aim of this research was to evaluate the E. coli resistance in strains by phenotypic methods, isolated from pet and stray dogs of La Plata city, Argentina. Faecal samples were collected using rectal swabs from 50 dogs with owners (home dogs = HD) and 50 homeless dogs (stray dogs = SD). They were cultured in 3 MacConkey agar plates, with and without antibiotics (ciprofloxacin and cefotaxime). 197 strains were isolated, of which only 95 strains were biochemically identified as E. coli, 46 strains were from HD, and 49 were from SD. Antimicrobial susceptibility was evaluated by the Kirby–Bauer disk diffusion method. The most prevalent resistance was for tetracycline, streptomycin, and ampicillin. In both groups, the level of resistance to 3rd generation cephalosporins was high, and there were multiresistant strains. There was a higher level of antimicrobial resistance in strains from SD compared to HD. There were 8% of strains suspected of being ESBLs among samples of HD and 36% of SD. One (2%) of the strains isolated from HD and 11 (22%) from SD were phenotypically confirmed as ESBL. Pets and stray dogs are a potential source of E. coli antibiotic resistance in Argentina; therefore, its surveillance must be guaranteed.
Facultad de Ciencias Veterinarias
Materia
Ciencias Veterinarias
Escherichia coli
Dogs
E. coli antibiotic resistance
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/123577
Acceder
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spelling Pet and Stray Dogs as Reservoirs of Antimicrobial-Resistant Escherichia coliMarchetti, María LauraBuldain, Daniel CornelioGortari Castillo, LihuelBuchamer, Andrea VerónicaChirino Trejo, ManuelMestorino, Olga NoraCiencias VeterinariasEscherichia coliDogsE. coli antibiotic resistanceThe close contact between dogs and humans creates the best bridge for interspecies transmission of antimicrobial-resistant bacteria. The surveillance of its resistance including the detection of extended-spectrum beta-lactamases (ESBLs) in Escherichia coli as indicator bacteria is an important tool to control the use of antimicrobials. The aim of this research was to evaluate the E. coli resistance in strains by phenotypic methods, isolated from pet and stray dogs of La Plata city, Argentina. Faecal samples were collected using rectal swabs from 50 dogs with owners (home dogs = HD) and 50 homeless dogs (stray dogs = SD). They were cultured in 3 MacConkey agar plates, with and without antibiotics (ciprofloxacin and cefotaxime). 197 strains were isolated, of which only 95 strains were biochemically identified as E. coli, 46 strains were from HD, and 49 were from SD. Antimicrobial susceptibility was evaluated by the Kirby–Bauer disk diffusion method. The most prevalent resistance was for tetracycline, streptomycin, and ampicillin. In both groups, the level of resistance to 3rd generation cephalosporins was high, and there were multiresistant strains. There was a higher level of antimicrobial resistance in strains from SD compared to HD. There were 8% of strains suspected of being ESBLs among samples of HD and 36% of SD. One (2%) of the strains isolated from HD and 11 (22%) from SD were phenotypically confirmed as ESBL. Pets and stray dogs are a potential source of E. coli antibiotic resistance in Argentina; therefore, its surveillance must be guaranteed.Facultad de Ciencias Veterinarias2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/123577enginfo:eu-repo/semantics/altIdentifier/issn/1687-918Xinfo:eu-repo/semantics/altIdentifier/issn/1687-9198info:eu-repo/semantics/altIdentifier/pmid/33564312info:eu-repo/semantics/altIdentifier/doi/10.1155/2021/6664557info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-22T17:10:24Zoai:sedici.unlp.edu.ar:10915/123577Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-22 17:10:24.593SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Pet and Stray Dogs as Reservoirs of Antimicrobial-Resistant Escherichia coli
title Pet and Stray Dogs as Reservoirs of Antimicrobial-Resistant Escherichia coli
spellingShingle Pet and Stray Dogs as Reservoirs of Antimicrobial-Resistant Escherichia coli
Marchetti, María Laura
Ciencias Veterinarias
Escherichia coli
Dogs
E. coli antibiotic resistance
title_short Pet and Stray Dogs as Reservoirs of Antimicrobial-Resistant Escherichia coli
title_full Pet and Stray Dogs as Reservoirs of Antimicrobial-Resistant Escherichia coli
title_fullStr Pet and Stray Dogs as Reservoirs of Antimicrobial-Resistant Escherichia coli
title_full_unstemmed Pet and Stray Dogs as Reservoirs of Antimicrobial-Resistant Escherichia coli
title_sort Pet and Stray Dogs as Reservoirs of Antimicrobial-Resistant Escherichia coli
dc.creator.none.fl_str_mv Marchetti, María Laura
Buldain, Daniel Cornelio
Gortari Castillo, Lihuel
Buchamer, Andrea Verónica
Chirino Trejo, Manuel
Mestorino, Olga Nora
author Marchetti, María Laura
author_facet Marchetti, María Laura
Buldain, Daniel Cornelio
Gortari Castillo, Lihuel
Buchamer, Andrea Verónica
Chirino Trejo, Manuel
Mestorino, Olga Nora
author_role author
author2 Buldain, Daniel Cornelio
Gortari Castillo, Lihuel
Buchamer, Andrea Verónica
Chirino Trejo, Manuel
Mestorino, Olga Nora
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Escherichia coli
Dogs
E. coli antibiotic resistance
topic Ciencias Veterinarias
Escherichia coli
Dogs
E. coli antibiotic resistance
dc.description.none.fl_txt_mv The close contact between dogs and humans creates the best bridge for interspecies transmission of antimicrobial-resistant bacteria. The surveillance of its resistance including the detection of extended-spectrum beta-lactamases (ESBLs) in Escherichia coli as indicator bacteria is an important tool to control the use of antimicrobials. The aim of this research was to evaluate the E. coli resistance in strains by phenotypic methods, isolated from pet and stray dogs of La Plata city, Argentina. Faecal samples were collected using rectal swabs from 50 dogs with owners (home dogs = HD) and 50 homeless dogs (stray dogs = SD). They were cultured in 3 MacConkey agar plates, with and without antibiotics (ciprofloxacin and cefotaxime). 197 strains were isolated, of which only 95 strains were biochemically identified as E. coli, 46 strains were from HD, and 49 were from SD. Antimicrobial susceptibility was evaluated by the Kirby–Bauer disk diffusion method. The most prevalent resistance was for tetracycline, streptomycin, and ampicillin. In both groups, the level of resistance to 3rd generation cephalosporins was high, and there were multiresistant strains. There was a higher level of antimicrobial resistance in strains from SD compared to HD. There were 8% of strains suspected of being ESBLs among samples of HD and 36% of SD. One (2%) of the strains isolated from HD and 11 (22%) from SD were phenotypically confirmed as ESBL. Pets and stray dogs are a potential source of E. coli antibiotic resistance in Argentina; therefore, its surveillance must be guaranteed.
Facultad de Ciencias Veterinarias
description The close contact between dogs and humans creates the best bridge for interspecies transmission of antimicrobial-resistant bacteria. The surveillance of its resistance including the detection of extended-spectrum beta-lactamases (ESBLs) in Escherichia coli as indicator bacteria is an important tool to control the use of antimicrobials. The aim of this research was to evaluate the E. coli resistance in strains by phenotypic methods, isolated from pet and stray dogs of La Plata city, Argentina. Faecal samples were collected using rectal swabs from 50 dogs with owners (home dogs = HD) and 50 homeless dogs (stray dogs = SD). They were cultured in 3 MacConkey agar plates, with and without antibiotics (ciprofloxacin and cefotaxime). 197 strains were isolated, of which only 95 strains were biochemically identified as E. coli, 46 strains were from HD, and 49 were from SD. Antimicrobial susceptibility was evaluated by the Kirby–Bauer disk diffusion method. The most prevalent resistance was for tetracycline, streptomycin, and ampicillin. In both groups, the level of resistance to 3rd generation cephalosporins was high, and there were multiresistant strains. There was a higher level of antimicrobial resistance in strains from SD compared to HD. There were 8% of strains suspected of being ESBLs among samples of HD and 36% of SD. One (2%) of the strains isolated from HD and 11 (22%) from SD were phenotypically confirmed as ESBL. Pets and stray dogs are a potential source of E. coli antibiotic resistance in Argentina; therefore, its surveillance must be guaranteed.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/123577
url http://sedici.unlp.edu.ar/handle/10915/123577
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1687-918X
info:eu-repo/semantics/altIdentifier/issn/1687-9198
info:eu-repo/semantics/altIdentifier/pmid/33564312
info:eu-repo/semantics/altIdentifier/doi/10.1155/2021/6664557
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
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instname:Universidad Nacional de La Plata
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instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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