Human serum albumin alters specific genes that can play a role in survival and persistence in Acinetobacter baumannii

Autores
Quinn, Brettni; Rodman, Nyah; Jara, Eugenio; Fernandez, Jennifer S.; Martinez, Jasmine; Traglia, German Matias; Montaña, Sabrina Daiana; Cantera, Virginia; Place, Kori; Bonomo, Robert A.; Iriante, Andrés; Ramirez, María Soledad
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the past few decades Acinetobacter baumannii has emerged as a notorious nosocomial pathogen because of its ability to acquire genetic material and persist in extreme environments. Recently, human serum albumin (HSA) was shown to significantly increase natural transformation frequency in A. baumannii. This observation led us to perform transcriptomic analysis of strain A118 under HSA induction to identify genes that are altered by HSA. Our results revealed the statistically significant differential expression of 296 protein-coding genes, including those associated with motility, biofilm formation, metabolism, efflux pumps, capsule synthesis, and transcriptional regulation. Phenotypic analysis of these traits showed an increase in surface-associated motility, a decrease in biofilm formation, reduced activity of a citric acid cycle associated enzyme, and increased survival associated with zinc availability. Furthermore, the expression of genes known to play a role in pathogenicity and antibiotic resistance were altered. These genes included those associated with RND-type efflux pumps, the type VI secretion system, iron acquisition/metabolism, and ß-lactam resistance. Together, these results illustrate how human products, in particular HSA, may play a significant role in both survival and persistence of A. baumannii.
Fil: Quinn, Brettni. California State University; Estados Unidos
Fil: Rodman, Nyah. California State University; Estados Unidos
Fil: Jara, Eugenio. Universidad de la República; Uruguay
Fil: Fernandez, Jennifer S.. California State University; Estados Unidos
Fil: Martinez, Jasmine. California State University; Estados Unidos
Fil: Traglia, German Matias. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Montaña, Sabrina Daiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Cantera, Virginia. Universidad de la República; Uruguay
Fil: Place, Kori. California State University; Estados Unidos
Fil: Bonomo, Robert A.. Case Western Reserve University; Estados Unidos. Louis Stokes Cleveland Department of Veterans Affair; Estados Unidos
Fil: Iriante, Andrés. Universidad de la República; Uruguay
Fil: Ramirez, María Soledad. California State University; Estados Unidos
Materia
HSA
Acinetobacter baumannii
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/90692
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/90692
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Human serum albumin alters specific genes that can play a role in survival and persistence in Acinetobacter baumanniiQuinn, BrettniRodman, NyahJara, EugenioFernandez, Jennifer S.Martinez, JasmineTraglia, German MatiasMontaña, Sabrina DaianaCantera, VirginiaPlace, KoriBonomo, Robert A.Iriante, AndrésRamirez, María SoledadHSAAcinetobacter baumanniihttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In the past few decades Acinetobacter baumannii has emerged as a notorious nosocomial pathogen because of its ability to acquire genetic material and persist in extreme environments. Recently, human serum albumin (HSA) was shown to significantly increase natural transformation frequency in A. baumannii. This observation led us to perform transcriptomic analysis of strain A118 under HSA induction to identify genes that are altered by HSA. Our results revealed the statistically significant differential expression of 296 protein-coding genes, including those associated with motility, biofilm formation, metabolism, efflux pumps, capsule synthesis, and transcriptional regulation. Phenotypic analysis of these traits showed an increase in surface-associated motility, a decrease in biofilm formation, reduced activity of a citric acid cycle associated enzyme, and increased survival associated with zinc availability. Furthermore, the expression of genes known to play a role in pathogenicity and antibiotic resistance were altered. These genes included those associated with RND-type efflux pumps, the type VI secretion system, iron acquisition/metabolism, and ß-lactam resistance. Together, these results illustrate how human products, in particular HSA, may play a significant role in both survival and persistence of A. baumannii.Fil: Quinn, Brettni. California State University; Estados UnidosFil: Rodman, Nyah. California State University; Estados UnidosFil: Jara, Eugenio. Universidad de la República; UruguayFil: Fernandez, Jennifer S.. California State University; Estados UnidosFil: Martinez, Jasmine. California State University; Estados UnidosFil: Traglia, German Matias. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Montaña, Sabrina Daiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Cantera, Virginia. Universidad de la República; UruguayFil: Place, Kori. California State University; Estados UnidosFil: Bonomo, Robert A.. Case Western Reserve University; Estados Unidos. Louis Stokes Cleveland Department of Veterans Affair; Estados UnidosFil: Iriante, Andrés. Universidad de la República; UruguayFil: Ramirez, María Soledad. California State University; Estados UnidosNature Publishing Group2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/90692Quinn, Brettni; Rodman, Nyah; Jara, Eugenio; Fernandez, Jennifer S.; Martinez, Jasmine; et al.; Human serum albumin alters specific genes that can play a role in survival and persistence in Acinetobacter baumannii; Nature Publishing Group; Scientific Reports; 8; 1; 12-2018; 1-162045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-018-33072-zinfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-018-33072-zinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:46Zoai:ri.conicet.gov.ar:11336/90692instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:46.857CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Human serum albumin alters specific genes that can play a role in survival and persistence in Acinetobacter baumannii
title Human serum albumin alters specific genes that can play a role in survival and persistence in Acinetobacter baumannii
spellingShingle Human serum albumin alters specific genes that can play a role in survival and persistence in Acinetobacter baumannii
Quinn, Brettni
HSA
Acinetobacter baumannii
title_short Human serum albumin alters specific genes that can play a role in survival and persistence in Acinetobacter baumannii
title_full Human serum albumin alters specific genes that can play a role in survival and persistence in Acinetobacter baumannii
title_fullStr Human serum albumin alters specific genes that can play a role in survival and persistence in Acinetobacter baumannii
title_full_unstemmed Human serum albumin alters specific genes that can play a role in survival and persistence in Acinetobacter baumannii
title_sort Human serum albumin alters specific genes that can play a role in survival and persistence in Acinetobacter baumannii
dc.creator.none.fl_str_mv Quinn, Brettni
Rodman, Nyah
Jara, Eugenio
Fernandez, Jennifer S.
Martinez, Jasmine
Traglia, German Matias
Montaña, Sabrina Daiana
Cantera, Virginia
Place, Kori
Bonomo, Robert A.
Iriante, Andrés
Ramirez, María Soledad
author Quinn, Brettni
author_facet Quinn, Brettni
Rodman, Nyah
Jara, Eugenio
Fernandez, Jennifer S.
Martinez, Jasmine
Traglia, German Matias
Montaña, Sabrina Daiana
Cantera, Virginia
Place, Kori
Bonomo, Robert A.
Iriante, Andrés
Ramirez, María Soledad
author_role author
author2 Rodman, Nyah
Jara, Eugenio
Fernandez, Jennifer S.
Martinez, Jasmine
Traglia, German Matias
Montaña, Sabrina Daiana
Cantera, Virginia
Place, Kori
Bonomo, Robert A.
Iriante, Andrés
Ramirez, María Soledad
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv HSA
Acinetobacter baumannii
topic HSA
Acinetobacter baumannii
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In the past few decades Acinetobacter baumannii has emerged as a notorious nosocomial pathogen because of its ability to acquire genetic material and persist in extreme environments. Recently, human serum albumin (HSA) was shown to significantly increase natural transformation frequency in A. baumannii. This observation led us to perform transcriptomic analysis of strain A118 under HSA induction to identify genes that are altered by HSA. Our results revealed the statistically significant differential expression of 296 protein-coding genes, including those associated with motility, biofilm formation, metabolism, efflux pumps, capsule synthesis, and transcriptional regulation. Phenotypic analysis of these traits showed an increase in surface-associated motility, a decrease in biofilm formation, reduced activity of a citric acid cycle associated enzyme, and increased survival associated with zinc availability. Furthermore, the expression of genes known to play a role in pathogenicity and antibiotic resistance were altered. These genes included those associated with RND-type efflux pumps, the type VI secretion system, iron acquisition/metabolism, and ß-lactam resistance. Together, these results illustrate how human products, in particular HSA, may play a significant role in both survival and persistence of A. baumannii.
Fil: Quinn, Brettni. California State University; Estados Unidos
Fil: Rodman, Nyah. California State University; Estados Unidos
Fil: Jara, Eugenio. Universidad de la República; Uruguay
Fil: Fernandez, Jennifer S.. California State University; Estados Unidos
Fil: Martinez, Jasmine. California State University; Estados Unidos
Fil: Traglia, German Matias. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Montaña, Sabrina Daiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Cantera, Virginia. Universidad de la República; Uruguay
Fil: Place, Kori. California State University; Estados Unidos
Fil: Bonomo, Robert A.. Case Western Reserve University; Estados Unidos. Louis Stokes Cleveland Department of Veterans Affair; Estados Unidos
Fil: Iriante, Andrés. Universidad de la República; Uruguay
Fil: Ramirez, María Soledad. California State University; Estados Unidos
description In the past few decades Acinetobacter baumannii has emerged as a notorious nosocomial pathogen because of its ability to acquire genetic material and persist in extreme environments. Recently, human serum albumin (HSA) was shown to significantly increase natural transformation frequency in A. baumannii. This observation led us to perform transcriptomic analysis of strain A118 under HSA induction to identify genes that are altered by HSA. Our results revealed the statistically significant differential expression of 296 protein-coding genes, including those associated with motility, biofilm formation, metabolism, efflux pumps, capsule synthesis, and transcriptional regulation. Phenotypic analysis of these traits showed an increase in surface-associated motility, a decrease in biofilm formation, reduced activity of a citric acid cycle associated enzyme, and increased survival associated with zinc availability. Furthermore, the expression of genes known to play a role in pathogenicity and antibiotic resistance were altered. These genes included those associated with RND-type efflux pumps, the type VI secretion system, iron acquisition/metabolism, and ß-lactam resistance. Together, these results illustrate how human products, in particular HSA, may play a significant role in both survival and persistence of A. baumannii.
publishDate 2018
dc.date.none.fl_str_mv 2018-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/90692
Quinn, Brettni; Rodman, Nyah; Jara, Eugenio; Fernandez, Jennifer S.; Martinez, Jasmine; et al.; Human serum albumin alters specific genes that can play a role in survival and persistence in Acinetobacter baumannii; Nature Publishing Group; Scientific Reports; 8; 1; 12-2018; 1-16
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/90692
identifier_str_mv Quinn, Brettni; Rodman, Nyah; Jara, Eugenio; Fernandez, Jennifer S.; Martinez, Jasmine; et al.; Human serum albumin alters specific genes that can play a role in survival and persistence in Acinetobacter baumannii; Nature Publishing Group; Scientific Reports; 8; 1; 12-2018; 1-16
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-018-33072-z
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-018-33072-z
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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