Controlling flowering of Medicago sativa (alfalfa) by inducing dominant mutations

Autores
Chiurazzi, Maurizio Junior; Nørrevang, Anton Frisgaard; García, Pedro; Cerdan, Pablo Diego; Palmgren, Michael; Wenkel, Stephan
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Breeding plants with polyploid genomes is challenging because functional redundancy hampers the identification of loss-of-function mutants. Medicago sativa is tetraploid and obligate outcrossing, which together with inbreeding depression complicates traditional breeding approaches in obtaining plants with a stable growth habit. Inducing dominant mutations would provide an alternative strategy to introduce domestication traits in plants with high gene redundancy. Here we describe two complementary strategies to induce dominant mutations in the M. sativa genome and how they can be relevant in the control of flowering time. First, we outline a genome-engineering strategy that harnesses the use of microProteins as developmental regulators. MicroProteins are small proteins that appeared during genome evolution from genes encoding larger proteins. Genome-engineering allows us to retrace evolution and create microProtein-coding genes de novo. Second, we provide an inventory of genes regulated by microRNAs that control plant development. Making respective gene transcripts microRNA-resistant by inducing point mutations can uncouple microRNA regulation. Finally, we investigated the recently published genomes of M. sativa and provide an inventory of breeding targets, some of which, when mutated, are likely to result in dominant traits.
Fil: Chiurazzi, Maurizio Junior. Universidad de Copenhagen; Dinamarca
Fil: Nørrevang, Anton Frisgaard. Universidad de Copenhagen; Dinamarca
Fil: García, Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Cerdan, Pablo Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Palmgren, Michael. Universidad de Copenhagen; Dinamarca
Fil: Wenkel, Stephan. Universidad de Copenhagen; Dinamarca
Materia
FLOWERING TIME
GENOME-ENGINEERING
MEDICAGO SATIVA
MICROPROTEIN
MICRORNA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/212758
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Controlling flowering of Medicago sativa (alfalfa) by inducing dominant mutationsChiurazzi, Maurizio JuniorNørrevang, Anton FrisgaardGarcía, PedroCerdan, Pablo DiegoPalmgren, MichaelWenkel, StephanFLOWERING TIMEGENOME-ENGINEERINGMEDICAGO SATIVAMICROPROTEINMICRORNAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Breeding plants with polyploid genomes is challenging because functional redundancy hampers the identification of loss-of-function mutants. Medicago sativa is tetraploid and obligate outcrossing, which together with inbreeding depression complicates traditional breeding approaches in obtaining plants with a stable growth habit. Inducing dominant mutations would provide an alternative strategy to introduce domestication traits in plants with high gene redundancy. Here we describe two complementary strategies to induce dominant mutations in the M. sativa genome and how they can be relevant in the control of flowering time. First, we outline a genome-engineering strategy that harnesses the use of microProteins as developmental regulators. MicroProteins are small proteins that appeared during genome evolution from genes encoding larger proteins. Genome-engineering allows us to retrace evolution and create microProtein-coding genes de novo. Second, we provide an inventory of genes regulated by microRNAs that control plant development. Making respective gene transcripts microRNA-resistant by inducing point mutations can uncouple microRNA regulation. Finally, we investigated the recently published genomes of M. sativa and provide an inventory of breeding targets, some of which, when mutated, are likely to result in dominant traits.Fil: Chiurazzi, Maurizio Junior. Universidad de Copenhagen; DinamarcaFil: Nørrevang, Anton Frisgaard. Universidad de Copenhagen; DinamarcaFil: García, Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Cerdan, Pablo Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Palmgren, Michael. Universidad de Copenhagen; DinamarcaFil: Wenkel, Stephan. Universidad de Copenhagen; DinamarcaWiley Blackwell Publishing, Inc2022-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/212758Chiurazzi, Maurizio Junior; Nørrevang, Anton Frisgaard; García, Pedro; Cerdan, Pablo Diego; Palmgren, Michael; et al.; Controlling flowering of Medicago sativa (alfalfa) by inducing dominant mutations; Wiley Blackwell Publishing, Inc; Journal Of Integrative Plant Biology; 64; 2; 2-2022; 205-2141672-9072CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/jipb.13186info:eu-repo/semantics/altIdentifier/doi/10.1111/jipb.13186info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:23:27Zoai:ri.conicet.gov.ar:11336/212758instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:23:27.729CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Controlling flowering of Medicago sativa (alfalfa) by inducing dominant mutations
title Controlling flowering of Medicago sativa (alfalfa) by inducing dominant mutations
spellingShingle Controlling flowering of Medicago sativa (alfalfa) by inducing dominant mutations
Chiurazzi, Maurizio Junior
FLOWERING TIME
GENOME-ENGINEERING
MEDICAGO SATIVA
MICROPROTEIN
MICRORNA
title_short Controlling flowering of Medicago sativa (alfalfa) by inducing dominant mutations
title_full Controlling flowering of Medicago sativa (alfalfa) by inducing dominant mutations
title_fullStr Controlling flowering of Medicago sativa (alfalfa) by inducing dominant mutations
title_full_unstemmed Controlling flowering of Medicago sativa (alfalfa) by inducing dominant mutations
title_sort Controlling flowering of Medicago sativa (alfalfa) by inducing dominant mutations
dc.creator.none.fl_str_mv Chiurazzi, Maurizio Junior
Nørrevang, Anton Frisgaard
García, Pedro
Cerdan, Pablo Diego
Palmgren, Michael
Wenkel, Stephan
author Chiurazzi, Maurizio Junior
author_facet Chiurazzi, Maurizio Junior
Nørrevang, Anton Frisgaard
García, Pedro
Cerdan, Pablo Diego
Palmgren, Michael
Wenkel, Stephan
author_role author
author2 Nørrevang, Anton Frisgaard
García, Pedro
Cerdan, Pablo Diego
Palmgren, Michael
Wenkel, Stephan
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv FLOWERING TIME
GENOME-ENGINEERING
MEDICAGO SATIVA
MICROPROTEIN
MICRORNA
topic FLOWERING TIME
GENOME-ENGINEERING
MEDICAGO SATIVA
MICROPROTEIN
MICRORNA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Breeding plants with polyploid genomes is challenging because functional redundancy hampers the identification of loss-of-function mutants. Medicago sativa is tetraploid and obligate outcrossing, which together with inbreeding depression complicates traditional breeding approaches in obtaining plants with a stable growth habit. Inducing dominant mutations would provide an alternative strategy to introduce domestication traits in plants with high gene redundancy. Here we describe two complementary strategies to induce dominant mutations in the M. sativa genome and how they can be relevant in the control of flowering time. First, we outline a genome-engineering strategy that harnesses the use of microProteins as developmental regulators. MicroProteins are small proteins that appeared during genome evolution from genes encoding larger proteins. Genome-engineering allows us to retrace evolution and create microProtein-coding genes de novo. Second, we provide an inventory of genes regulated by microRNAs that control plant development. Making respective gene transcripts microRNA-resistant by inducing point mutations can uncouple microRNA regulation. Finally, we investigated the recently published genomes of M. sativa and provide an inventory of breeding targets, some of which, when mutated, are likely to result in dominant traits.
Fil: Chiurazzi, Maurizio Junior. Universidad de Copenhagen; Dinamarca
Fil: Nørrevang, Anton Frisgaard. Universidad de Copenhagen; Dinamarca
Fil: García, Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Cerdan, Pablo Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Palmgren, Michael. Universidad de Copenhagen; Dinamarca
Fil: Wenkel, Stephan. Universidad de Copenhagen; Dinamarca
description Breeding plants with polyploid genomes is challenging because functional redundancy hampers the identification of loss-of-function mutants. Medicago sativa is tetraploid and obligate outcrossing, which together with inbreeding depression complicates traditional breeding approaches in obtaining plants with a stable growth habit. Inducing dominant mutations would provide an alternative strategy to introduce domestication traits in plants with high gene redundancy. Here we describe two complementary strategies to induce dominant mutations in the M. sativa genome and how they can be relevant in the control of flowering time. First, we outline a genome-engineering strategy that harnesses the use of microProteins as developmental regulators. MicroProteins are small proteins that appeared during genome evolution from genes encoding larger proteins. Genome-engineering allows us to retrace evolution and create microProtein-coding genes de novo. Second, we provide an inventory of genes regulated by microRNAs that control plant development. Making respective gene transcripts microRNA-resistant by inducing point mutations can uncouple microRNA regulation. Finally, we investigated the recently published genomes of M. sativa and provide an inventory of breeding targets, some of which, when mutated, are likely to result in dominant traits.
publishDate 2022
dc.date.none.fl_str_mv 2022-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/212758
Chiurazzi, Maurizio Junior; Nørrevang, Anton Frisgaard; García, Pedro; Cerdan, Pablo Diego; Palmgren, Michael; et al.; Controlling flowering of Medicago sativa (alfalfa) by inducing dominant mutations; Wiley Blackwell Publishing, Inc; Journal Of Integrative Plant Biology; 64; 2; 2-2022; 205-214
1672-9072
CONICET Digital
CONICET
url http://hdl.handle.net/11336/212758
identifier_str_mv Chiurazzi, Maurizio Junior; Nørrevang, Anton Frisgaard; García, Pedro; Cerdan, Pablo Diego; Palmgren, Michael; et al.; Controlling flowering of Medicago sativa (alfalfa) by inducing dominant mutations; Wiley Blackwell Publishing, Inc; Journal Of Integrative Plant Biology; 64; 2; 2-2022; 205-214
1672-9072
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/jipb.13186
info:eu-repo/semantics/altIdentifier/doi/10.1111/jipb.13186
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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