Evaluation of Synthetic and Semi- synthetic Culture Media for Endo-1,4-β- Glucanases Secretion by Trichoderma koningiopsis

Autores
Castrillo, María Lorena; Bich, Gustavo Angel; Kramer, Gustavo Raul; Velázquez, Juan Ernesto; Rodriguez, M.; Zapata, Pedro Dario; Villalba, Laura
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The actual demand of energy and the environmental concerns together with the reduced fossil fuel reserves have played an important role to convert the second generation bioethanol production into an attractive research area. To convert lignocellulosic biomass to bioethanol the cellulosic components must be hydrolyzed to fermentable sugars. Trichoderma fungi secrete large amounts of enzymes of industrial interest such as cellulases, able to degrade holocellulose in the saccharification of lignocellulosic biomass. In this work we evaluated endo-1.4-β-glucanases enzymatic secretion of Trichoderma koningiopsis from Misiones province, in synthetic medium, with carboxymethylcellulose as carbon source; and semi-synthetic medium, with pine sawdust as carbon source. Higher values of endo-1.4-β-glucanases were reached when the semi-synthetic medium was used. It could be concluded that pine sawdust seems to be a good candidate for utilization as carbon source in culture media aiming to obtain good enzyme secretion, being also an economic and easily available substrate.
Fil: Castrillo, María Lorena. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bich, Gustavo Angel. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kramer, Gustavo Raul. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Velázquez, Juan Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina
Fil: Rodriguez, M.. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina
Fil: Zapata, Pedro Dario. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Villalba, Laura. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina
Materia
TRICHODERMA
CELLULASES
SYNTHETIC AND SEMI- SYNTHETIC CULTURE MEDIA
ENDO-1,4-Β- GLUCANASES
CARBOXYMETHYLCELLULOSE
PINE SAWDUST
TRICHODERMA KONINGIOPSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/37460
Acceder
id CONICETDig_d22e0ec7d3f4b62c7a9c3088160646ff
oai_identifier_str oai:ri.conicet.gov.ar:11336/37460
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of Synthetic and Semi- synthetic Culture Media for Endo-1,4-β- Glucanases Secretion by Trichoderma koningiopsisCastrillo, María LorenaBich, Gustavo AngelKramer, Gustavo RaulVelázquez, Juan ErnestoRodriguez, M.Zapata, Pedro DarioVillalba, LauraTRICHODERMACELLULASESSYNTHETIC AND SEMI- SYNTHETIC CULTURE MEDIAENDO-1,4-Β- GLUCANASESCARBOXYMETHYLCELLULOSEPINE SAWDUSTTRICHODERMA KONINGIOPSIShttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2The actual demand of energy and the environmental concerns together with the reduced fossil fuel reserves have played an important role to convert the second generation bioethanol production into an attractive research area. To convert lignocellulosic biomass to bioethanol the cellulosic components must be hydrolyzed to fermentable sugars. Trichoderma fungi secrete large amounts of enzymes of industrial interest such as cellulases, able to degrade holocellulose in the saccharification of lignocellulosic biomass. In this work we evaluated endo-1.4-β-glucanases enzymatic secretion of Trichoderma koningiopsis from Misiones province, in synthetic medium, with carboxymethylcellulose as carbon source; and semi-synthetic medium, with pine sawdust as carbon source. Higher values of endo-1.4-β-glucanases were reached when the semi-synthetic medium was used. It could be concluded that pine sawdust seems to be a good candidate for utilization as carbon source in culture media aiming to obtain good enzyme secretion, being also an economic and easily available substrate.Fil: Castrillo, María Lorena. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bich, Gustavo Angel. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Kramer, Gustavo Raul. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Velázquez, Juan Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; ArgentinaFil: Rodriguez, M.. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; ArgentinaFil: Zapata, Pedro Dario. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Villalba, Laura. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; ArgentinaElsevier2015-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/37460Castrillo, María Lorena; Bich, Gustavo Angel; Kramer, Gustavo Raul; Velázquez, Juan Ernesto; Rodriguez, M.; et al.; Evaluation of Synthetic and Semi- synthetic Culture Media for Endo-1,4-β- Glucanases Secretion by Trichoderma koningiopsis; Elsevier; Procedia Materials Science; 8; 6-2015; 786-7922211-8128CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2211812815001376info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mspro.2015.04.136info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:00:29Zoai:ri.conicet.gov.ar:11336/37460instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:00:29.269CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of Synthetic and Semi- synthetic Culture Media for Endo-1,4-β- Glucanases Secretion by Trichoderma koningiopsis
title Evaluation of Synthetic and Semi- synthetic Culture Media for Endo-1,4-β- Glucanases Secretion by Trichoderma koningiopsis
spellingShingle Evaluation of Synthetic and Semi- synthetic Culture Media for Endo-1,4-β- Glucanases Secretion by Trichoderma koningiopsis
Castrillo, María Lorena
TRICHODERMA
CELLULASES
SYNTHETIC AND SEMI- SYNTHETIC CULTURE MEDIA
ENDO-1,4-Β- GLUCANASES
CARBOXYMETHYLCELLULOSE
PINE SAWDUST
TRICHODERMA KONINGIOPSIS
title_short Evaluation of Synthetic and Semi- synthetic Culture Media for Endo-1,4-β- Glucanases Secretion by Trichoderma koningiopsis
title_full Evaluation of Synthetic and Semi- synthetic Culture Media for Endo-1,4-β- Glucanases Secretion by Trichoderma koningiopsis
title_fullStr Evaluation of Synthetic and Semi- synthetic Culture Media for Endo-1,4-β- Glucanases Secretion by Trichoderma koningiopsis
title_full_unstemmed Evaluation of Synthetic and Semi- synthetic Culture Media for Endo-1,4-β- Glucanases Secretion by Trichoderma koningiopsis
title_sort Evaluation of Synthetic and Semi- synthetic Culture Media for Endo-1,4-β- Glucanases Secretion by Trichoderma koningiopsis
dc.creator.none.fl_str_mv Castrillo, María Lorena
Bich, Gustavo Angel
Kramer, Gustavo Raul
Velázquez, Juan Ernesto
Rodriguez, M.
Zapata, Pedro Dario
Villalba, Laura
author Castrillo, María Lorena
author_facet Castrillo, María Lorena
Bich, Gustavo Angel
Kramer, Gustavo Raul
Velázquez, Juan Ernesto
Rodriguez, M.
Zapata, Pedro Dario
Villalba, Laura
author_role author
author2 Bich, Gustavo Angel
Kramer, Gustavo Raul
Velázquez, Juan Ernesto
Rodriguez, M.
Zapata, Pedro Dario
Villalba, Laura
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv TRICHODERMA
CELLULASES
SYNTHETIC AND SEMI- SYNTHETIC CULTURE MEDIA
ENDO-1,4-Β- GLUCANASES
CARBOXYMETHYLCELLULOSE
PINE SAWDUST
TRICHODERMA KONINGIOPSIS
topic TRICHODERMA
CELLULASES
SYNTHETIC AND SEMI- SYNTHETIC CULTURE MEDIA
ENDO-1,4-Β- GLUCANASES
CARBOXYMETHYLCELLULOSE
PINE SAWDUST
TRICHODERMA KONINGIOPSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The actual demand of energy and the environmental concerns together with the reduced fossil fuel reserves have played an important role to convert the second generation bioethanol production into an attractive research area. To convert lignocellulosic biomass to bioethanol the cellulosic components must be hydrolyzed to fermentable sugars. Trichoderma fungi secrete large amounts of enzymes of industrial interest such as cellulases, able to degrade holocellulose in the saccharification of lignocellulosic biomass. In this work we evaluated endo-1.4-β-glucanases enzymatic secretion of Trichoderma koningiopsis from Misiones province, in synthetic medium, with carboxymethylcellulose as carbon source; and semi-synthetic medium, with pine sawdust as carbon source. Higher values of endo-1.4-β-glucanases were reached when the semi-synthetic medium was used. It could be concluded that pine sawdust seems to be a good candidate for utilization as carbon source in culture media aiming to obtain good enzyme secretion, being also an economic and easily available substrate.
Fil: Castrillo, María Lorena. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bich, Gustavo Angel. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kramer, Gustavo Raul. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Velázquez, Juan Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina
Fil: Rodriguez, M.. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina
Fil: Zapata, Pedro Dario. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Villalba, Laura. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina
description The actual demand of energy and the environmental concerns together with the reduced fossil fuel reserves have played an important role to convert the second generation bioethanol production into an attractive research area. To convert lignocellulosic biomass to bioethanol the cellulosic components must be hydrolyzed to fermentable sugars. Trichoderma fungi secrete large amounts of enzymes of industrial interest such as cellulases, able to degrade holocellulose in the saccharification of lignocellulosic biomass. In this work we evaluated endo-1.4-β-glucanases enzymatic secretion of Trichoderma koningiopsis from Misiones province, in synthetic medium, with carboxymethylcellulose as carbon source; and semi-synthetic medium, with pine sawdust as carbon source. Higher values of endo-1.4-β-glucanases were reached when the semi-synthetic medium was used. It could be concluded that pine sawdust seems to be a good candidate for utilization as carbon source in culture media aiming to obtain good enzyme secretion, being also an economic and easily available substrate.
publishDate 2015
dc.date.none.fl_str_mv 2015-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/37460
Castrillo, María Lorena; Bich, Gustavo Angel; Kramer, Gustavo Raul; Velázquez, Juan Ernesto; Rodriguez, M.; et al.; Evaluation of Synthetic and Semi- synthetic Culture Media for Endo-1,4-β- Glucanases Secretion by Trichoderma koningiopsis; Elsevier; Procedia Materials Science; 8; 6-2015; 786-792
2211-8128
CONICET Digital
CONICET
url http://hdl.handle.net/11336/37460
identifier_str_mv Castrillo, María Lorena; Bich, Gustavo Angel; Kramer, Gustavo Raul; Velázquez, Juan Ernesto; Rodriguez, M.; et al.; Evaluation of Synthetic and Semi- synthetic Culture Media for Endo-1,4-β- Glucanases Secretion by Trichoderma koningiopsis; Elsevier; Procedia Materials Science; 8; 6-2015; 786-792
2211-8128
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2211812815001376
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mspro.2015.04.136
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842979884280840192
score 12.993085

Publicaciones similares