Aspergillus niger LBM 134 isolated from rotten wood and its potential cellulolytic ability

Autores
Díaz, Gabriela Verónica; Coniglio, Romina Olga; Chungara, Clara Inés; Zapata, Pedro Dario; Villalba, Laura Lidia; Fonseca, Maria Isabel
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aspergillus: is a genus of filamentous and cosmopolitan fungi that includes important species for medical mycology, food, basic research and agro-industry areas. Aspergillus section Nigri are efficient producers of hydrolytic enzymes such as cellulases that are employed in the cellulose conversion. Hence, the search of new cellulolytic isolates and their correct identification is important for carrying out safe biotechnological processes. This study aimed to characterise the cellulolytic potential of Aspergillus sp. LBM 134, isolated from the Paranaense rainforest (Argentina) and to identify the isolate through a polyphasic approach. The fungus was identified as Aspergillus niger and its cellulolytic potential was evaluated by using Congo red technique and fluorescence plate assays for carboxymethyl cellulase, β-glucosidase and cellobiohydrolase, respectively. All three cellulase activities were positive; this bio-prospective positioned A. niger LBM 134 as a promising alternative for industries that require organisms capable of carrying out cellulosic biomass processing.
Fil: Díaz, Gabriela Verónica. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Coniglio, Romina Olga. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones; Argentina
Fil: Chungara, Clara Inés. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Zapata, Pedro Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones; Argentina
Fil: Villalba, Laura Lidia. Universidad Nacional de Misiones; Argentina
Fil: Fonseca, Maria Isabel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Materia
CELLULASES
FLUORESCENCE ASSAYS
MACROMORPHOLOGY
MICROMORPHOLOGY
MOLECULAR MARKERS
NIGRI
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/126772
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/126772
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Aspergillus niger LBM 134 isolated from rotten wood and its potential cellulolytic abilityDíaz, Gabriela VerónicaConiglio, Romina OlgaChungara, Clara InésZapata, Pedro DarioVillalba, Laura LidiaFonseca, Maria IsabelCELLULASESFLUORESCENCE ASSAYSMACROMORPHOLOGYMICROMORPHOLOGYMOLECULAR MARKERSNIGRIhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Aspergillus: is a genus of filamentous and cosmopolitan fungi that includes important species for medical mycology, food, basic research and agro-industry areas. Aspergillus section Nigri are efficient producers of hydrolytic enzymes such as cellulases that are employed in the cellulose conversion. Hence, the search of new cellulolytic isolates and their correct identification is important for carrying out safe biotechnological processes. This study aimed to characterise the cellulolytic potential of Aspergillus sp. LBM 134, isolated from the Paranaense rainforest (Argentina) and to identify the isolate through a polyphasic approach. The fungus was identified as Aspergillus niger and its cellulolytic potential was evaluated by using Congo red technique and fluorescence plate assays for carboxymethyl cellulase, β-glucosidase and cellobiohydrolase, respectively. All three cellulase activities were positive; this bio-prospective positioned A. niger LBM 134 as a promising alternative for industries that require organisms capable of carrying out cellulosic biomass processing.Fil: Díaz, Gabriela Verónica. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Coniglio, Romina Olga. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones; ArgentinaFil: Chungara, Clara Inés. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaFil: Zapata, Pedro Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones; ArgentinaFil: Villalba, Laura Lidia. Universidad Nacional de Misiones; ArgentinaFil: Fonseca, Maria Isabel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaTaylor & Francis2020-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/126772Díaz, Gabriela Verónica; Coniglio, Romina Olga; Chungara, Clara Inés; Zapata, Pedro Dario; Villalba, Laura Lidia; et al.; Aspergillus niger LBM 134 isolated from rotten wood and its potential cellulolytic ability; Taylor & Francis; Mycology; 2020; 9-2020; 1-142150-12032150-1211CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/21501203.2020.1823509info:eu-repo/semantics/altIdentifier/doi/10.1080/21501203.2020.1823509info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:51:51Zoai:ri.conicet.gov.ar:11336/126772instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:51:52.003CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Aspergillus niger LBM 134 isolated from rotten wood and its potential cellulolytic ability
title Aspergillus niger LBM 134 isolated from rotten wood and its potential cellulolytic ability
spellingShingle Aspergillus niger LBM 134 isolated from rotten wood and its potential cellulolytic ability
Díaz, Gabriela Verónica
CELLULASES
FLUORESCENCE ASSAYS
MACROMORPHOLOGY
MICROMORPHOLOGY
MOLECULAR MARKERS
NIGRI
title_short Aspergillus niger LBM 134 isolated from rotten wood and its potential cellulolytic ability
title_full Aspergillus niger LBM 134 isolated from rotten wood and its potential cellulolytic ability
title_fullStr Aspergillus niger LBM 134 isolated from rotten wood and its potential cellulolytic ability
title_full_unstemmed Aspergillus niger LBM 134 isolated from rotten wood and its potential cellulolytic ability
title_sort Aspergillus niger LBM 134 isolated from rotten wood and its potential cellulolytic ability
dc.creator.none.fl_str_mv Díaz, Gabriela Verónica
Coniglio, Romina Olga
Chungara, Clara Inés
Zapata, Pedro Dario
Villalba, Laura Lidia
Fonseca, Maria Isabel
author Díaz, Gabriela Verónica
author_facet Díaz, Gabriela Verónica
Coniglio, Romina Olga
Chungara, Clara Inés
Zapata, Pedro Dario
Villalba, Laura Lidia
Fonseca, Maria Isabel
author_role author
author2 Coniglio, Romina Olga
Chungara, Clara Inés
Zapata, Pedro Dario
Villalba, Laura Lidia
Fonseca, Maria Isabel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CELLULASES
FLUORESCENCE ASSAYS
MACROMORPHOLOGY
MICROMORPHOLOGY
MOLECULAR MARKERS
NIGRI
topic CELLULASES
FLUORESCENCE ASSAYS
MACROMORPHOLOGY
MICROMORPHOLOGY
MOLECULAR MARKERS
NIGRI
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Aspergillus: is a genus of filamentous and cosmopolitan fungi that includes important species for medical mycology, food, basic research and agro-industry areas. Aspergillus section Nigri are efficient producers of hydrolytic enzymes such as cellulases that are employed in the cellulose conversion. Hence, the search of new cellulolytic isolates and their correct identification is important for carrying out safe biotechnological processes. This study aimed to characterise the cellulolytic potential of Aspergillus sp. LBM 134, isolated from the Paranaense rainforest (Argentina) and to identify the isolate through a polyphasic approach. The fungus was identified as Aspergillus niger and its cellulolytic potential was evaluated by using Congo red technique and fluorescence plate assays for carboxymethyl cellulase, β-glucosidase and cellobiohydrolase, respectively. All three cellulase activities were positive; this bio-prospective positioned A. niger LBM 134 as a promising alternative for industries that require organisms capable of carrying out cellulosic biomass processing.
Fil: Díaz, Gabriela Verónica. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Coniglio, Romina Olga. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones; Argentina
Fil: Chungara, Clara Inés. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Zapata, Pedro Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones; Argentina
Fil: Villalba, Laura Lidia. Universidad Nacional de Misiones; Argentina
Fil: Fonseca, Maria Isabel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
description Aspergillus: is a genus of filamentous and cosmopolitan fungi that includes important species for medical mycology, food, basic research and agro-industry areas. Aspergillus section Nigri are efficient producers of hydrolytic enzymes such as cellulases that are employed in the cellulose conversion. Hence, the search of new cellulolytic isolates and their correct identification is important for carrying out safe biotechnological processes. This study aimed to characterise the cellulolytic potential of Aspergillus sp. LBM 134, isolated from the Paranaense rainforest (Argentina) and to identify the isolate through a polyphasic approach. The fungus was identified as Aspergillus niger and its cellulolytic potential was evaluated by using Congo red technique and fluorescence plate assays for carboxymethyl cellulase, β-glucosidase and cellobiohydrolase, respectively. All three cellulase activities were positive; this bio-prospective positioned A. niger LBM 134 as a promising alternative for industries that require organisms capable of carrying out cellulosic biomass processing.
publishDate 2020
dc.date.none.fl_str_mv 2020-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/126772
Díaz, Gabriela Verónica; Coniglio, Romina Olga; Chungara, Clara Inés; Zapata, Pedro Dario; Villalba, Laura Lidia; et al.; Aspergillus niger LBM 134 isolated from rotten wood and its potential cellulolytic ability; Taylor & Francis; Mycology; 2020; 9-2020; 1-14
2150-1203
2150-1211
CONICET Digital
CONICET
url http://hdl.handle.net/11336/126772
identifier_str_mv Díaz, Gabriela Verónica; Coniglio, Romina Olga; Chungara, Clara Inés; Zapata, Pedro Dario; Villalba, Laura Lidia; et al.; Aspergillus niger LBM 134 isolated from rotten wood and its potential cellulolytic ability; Taylor & Francis; Mycology; 2020; 9-2020; 1-14
2150-1203
2150-1211
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/21501203.2020.1823509
info:eu-repo/semantics/altIdentifier/doi/10.1080/21501203.2020.1823509
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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