Aqueous extract from Opuntia megapotamica fruit pulp promotes osteoblast activity
- Autores
- Gili, Valeria; Laiuppa, Juan Andrés; Santillán, Graciela Edith
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Objetive: Osteoporosis and osteopenia are age-related chronic diseases with increased morbidity rates among postmenopausal women. Natural productsinvestigation for prevention and treatment of these conditions is growing, due, in part, to the fact that they allow longer exposure times and minor secondary complications than synthetic drugs. Opuntia plants (Cactaceae) have been shown to possess a broad spectrum of medicinal properties. Opuntia megapotamica is an autochthonous species from Argentina but its action on bone metabolism has not yet been studied. The work aims is to evaluate the action of aqueous extracts of O. megapotamica fruits on the activity and function of osteoblasts in vitro.Methods: Primary osteoblasts were obtained by digestion of the cranial bones of 3-5 day-old Wistar rats. Cells were treated with different dilutions of the aqueous extract (1/50-1/1000), then viability, proliferation, differentiation and mineralization were assessed at the corresponding time points.Results: Dilution of extract 1/100 induced a slight but signifi cant increase in cell viability with respect to control (p< 0.05) whereas the other conditions showed no changes. In addition, dilutions of extract 1/100 and 1/1000 signifi cantly increased cell proliferation at 24 and 48 h with respect to the control (p< 0.05). Culture mineralization, showed a rising trend with respect to the control at 15 days of treatment with 1/1000 extract dilution.Conclusions: The results show that the aqueous extract of O. megapotamica fruit increases the number and improves the biological functions of osteoblasts,suggesting its potential use as an alternative treatment for postmenopausal bone deficit.
Fil: Gili, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Laiuppa, Juan Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Santillán, Graciela Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina - Materia
-
OSTEOBLAST
OPUNTIA
POSTMENOPAUSAL OSTEOPOROSIS
OSTEOPENIA - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/141503
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Aqueous extract from Opuntia megapotamica fruit pulp promotes osteoblast activityGili, ValeriaLaiuppa, Juan AndrésSantillán, Graciela EdithOSTEOBLASTOPUNTIAPOSTMENOPAUSAL OSTEOPOROSISOSTEOPENIAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Objetive: Osteoporosis and osteopenia are age-related chronic diseases with increased morbidity rates among postmenopausal women. Natural productsinvestigation for prevention and treatment of these conditions is growing, due, in part, to the fact that they allow longer exposure times and minor secondary complications than synthetic drugs. Opuntia plants (Cactaceae) have been shown to possess a broad spectrum of medicinal properties. Opuntia megapotamica is an autochthonous species from Argentina but its action on bone metabolism has not yet been studied. The work aims is to evaluate the action of aqueous extracts of O. megapotamica fruits on the activity and function of osteoblasts in vitro.Methods: Primary osteoblasts were obtained by digestion of the cranial bones of 3-5 day-old Wistar rats. Cells were treated with different dilutions of the aqueous extract (1/50-1/1000), then viability, proliferation, differentiation and mineralization were assessed at the corresponding time points.Results: Dilution of extract 1/100 induced a slight but signifi cant increase in cell viability with respect to control (p< 0.05) whereas the other conditions showed no changes. In addition, dilutions of extract 1/100 and 1/1000 signifi cantly increased cell proliferation at 24 and 48 h with respect to the control (p< 0.05). Culture mineralization, showed a rising trend with respect to the control at 15 days of treatment with 1/1000 extract dilution.Conclusions: The results show that the aqueous extract of O. megapotamica fruit increases the number and improves the biological functions of osteoblasts,suggesting its potential use as an alternative treatment for postmenopausal bone deficit.Fil: Gili, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Laiuppa, Juan Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Santillán, Graciela Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaPeertechz Publications2020-05-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/141503Gili, Valeria; Laiuppa, Juan Andrés; Santillán, Graciela Edith; Aqueous extract from Opuntia megapotamica fruit pulp promotes osteoblast activity; Peertechz Publications; International Journal of Spine Research; 2; 1; 20-5-2020; 37-452692-4692CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.17352/ijsr.000012info:eu-repo/semantics/altIdentifier/url/https://www.peertechzpublications.com/articles/IJSR-2-112.phpinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:21Zoai:ri.conicet.gov.ar:11336/141503instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:21.577CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Aqueous extract from Opuntia megapotamica fruit pulp promotes osteoblast activity |
title |
Aqueous extract from Opuntia megapotamica fruit pulp promotes osteoblast activity |
spellingShingle |
Aqueous extract from Opuntia megapotamica fruit pulp promotes osteoblast activity Gili, Valeria OSTEOBLAST OPUNTIA POSTMENOPAUSAL OSTEOPOROSIS OSTEOPENIA |
title_short |
Aqueous extract from Opuntia megapotamica fruit pulp promotes osteoblast activity |
title_full |
Aqueous extract from Opuntia megapotamica fruit pulp promotes osteoblast activity |
title_fullStr |
Aqueous extract from Opuntia megapotamica fruit pulp promotes osteoblast activity |
title_full_unstemmed |
Aqueous extract from Opuntia megapotamica fruit pulp promotes osteoblast activity |
title_sort |
Aqueous extract from Opuntia megapotamica fruit pulp promotes osteoblast activity |
dc.creator.none.fl_str_mv |
Gili, Valeria Laiuppa, Juan Andrés Santillán, Graciela Edith |
author |
Gili, Valeria |
author_facet |
Gili, Valeria Laiuppa, Juan Andrés Santillán, Graciela Edith |
author_role |
author |
author2 |
Laiuppa, Juan Andrés Santillán, Graciela Edith |
author2_role |
author author |
dc.subject.none.fl_str_mv |
OSTEOBLAST OPUNTIA POSTMENOPAUSAL OSTEOPOROSIS OSTEOPENIA |
topic |
OSTEOBLAST OPUNTIA POSTMENOPAUSAL OSTEOPOROSIS OSTEOPENIA |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Objetive: Osteoporosis and osteopenia are age-related chronic diseases with increased morbidity rates among postmenopausal women. Natural productsinvestigation for prevention and treatment of these conditions is growing, due, in part, to the fact that they allow longer exposure times and minor secondary complications than synthetic drugs. Opuntia plants (Cactaceae) have been shown to possess a broad spectrum of medicinal properties. Opuntia megapotamica is an autochthonous species from Argentina but its action on bone metabolism has not yet been studied. The work aims is to evaluate the action of aqueous extracts of O. megapotamica fruits on the activity and function of osteoblasts in vitro.Methods: Primary osteoblasts were obtained by digestion of the cranial bones of 3-5 day-old Wistar rats. Cells were treated with different dilutions of the aqueous extract (1/50-1/1000), then viability, proliferation, differentiation and mineralization were assessed at the corresponding time points.Results: Dilution of extract 1/100 induced a slight but signifi cant increase in cell viability with respect to control (p< 0.05) whereas the other conditions showed no changes. In addition, dilutions of extract 1/100 and 1/1000 signifi cantly increased cell proliferation at 24 and 48 h with respect to the control (p< 0.05). Culture mineralization, showed a rising trend with respect to the control at 15 days of treatment with 1/1000 extract dilution.Conclusions: The results show that the aqueous extract of O. megapotamica fruit increases the number and improves the biological functions of osteoblasts,suggesting its potential use as an alternative treatment for postmenopausal bone deficit. Fil: Gili, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina Fil: Laiuppa, Juan Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina Fil: Santillán, Graciela Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina |
description |
Objetive: Osteoporosis and osteopenia are age-related chronic diseases with increased morbidity rates among postmenopausal women. Natural productsinvestigation for prevention and treatment of these conditions is growing, due, in part, to the fact that they allow longer exposure times and minor secondary complications than synthetic drugs. Opuntia plants (Cactaceae) have been shown to possess a broad spectrum of medicinal properties. Opuntia megapotamica is an autochthonous species from Argentina but its action on bone metabolism has not yet been studied. The work aims is to evaluate the action of aqueous extracts of O. megapotamica fruits on the activity and function of osteoblasts in vitro.Methods: Primary osteoblasts were obtained by digestion of the cranial bones of 3-5 day-old Wistar rats. Cells were treated with different dilutions of the aqueous extract (1/50-1/1000), then viability, proliferation, differentiation and mineralization were assessed at the corresponding time points.Results: Dilution of extract 1/100 induced a slight but signifi cant increase in cell viability with respect to control (p< 0.05) whereas the other conditions showed no changes. In addition, dilutions of extract 1/100 and 1/1000 signifi cantly increased cell proliferation at 24 and 48 h with respect to the control (p< 0.05). Culture mineralization, showed a rising trend with respect to the control at 15 days of treatment with 1/1000 extract dilution.Conclusions: The results show that the aqueous extract of O. megapotamica fruit increases the number and improves the biological functions of osteoblasts,suggesting its potential use as an alternative treatment for postmenopausal bone deficit. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-05-20 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/141503 Gili, Valeria; Laiuppa, Juan Andrés; Santillán, Graciela Edith; Aqueous extract from Opuntia megapotamica fruit pulp promotes osteoblast activity; Peertechz Publications; International Journal of Spine Research; 2; 1; 20-5-2020; 37-45 2692-4692 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/141503 |
identifier_str_mv |
Gili, Valeria; Laiuppa, Juan Andrés; Santillán, Graciela Edith; Aqueous extract from Opuntia megapotamica fruit pulp promotes osteoblast activity; Peertechz Publications; International Journal of Spine Research; 2; 1; 20-5-2020; 37-45 2692-4692 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.17352/ijsr.000012 info:eu-repo/semantics/altIdentifier/url/https://www.peertechzpublications.com/articles/IJSR-2-112.php |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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openAccess |
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Peertechz Publications |
publisher.none.fl_str_mv |
Peertechz Publications |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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