Aqueous extract from Opuntia megapotamica fruit pulp promotes osteoblast activity

Autores
Gili, Valeria; Laiuppa, Juan Andrés; Santillán, Graciela Edith
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objetive: Osteoporosis and osteopenia are age-related chronic diseases with increased morbidity rates among postmenopausal women. Natural productsinvestigation for prevention and treatment of these conditions is growing, due, in part, to the fact that they allow longer exposure times and minor secondary complications than synthetic drugs. Opuntia plants (Cactaceae) have been shown to possess a broad spectrum of medicinal properties. Opuntia megapotamica is an autochthonous species from Argentina but its action on bone metabolism has not yet been studied. The work aims is to evaluate the action of aqueous extracts of O. megapotamica fruits on the activity and function of osteoblasts in vitro.Methods: Primary osteoblasts were obtained by digestion of the cranial bones of 3-5 day-old Wistar rats. Cells were treated with different dilutions of the aqueous extract (1/50-1/1000), then viability, proliferation, differentiation and mineralization were assessed at the corresponding time points.Results: Dilution of extract 1/100 induced a slight but signifi cant increase in cell viability with respect to control (p< 0.05) whereas the other conditions showed no changes. In addition, dilutions of extract 1/100 and 1/1000 signifi cantly increased cell proliferation at 24 and 48 h with respect to the control (p< 0.05). Culture mineralization, showed a rising trend with respect to the control at 15 days of treatment with 1/1000 extract dilution.Conclusions: The results show that the aqueous extract of O. megapotamica fruit increases the number and improves the biological functions of osteoblasts,suggesting its potential use as an alternative treatment for postmenopausal bone deficit.
Fil: Gili, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Laiuppa, Juan Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Santillán, Graciela Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Materia
OSTEOBLAST
OPUNTIA
POSTMENOPAUSAL OSTEOPOROSIS
OSTEOPENIA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/141503
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Aqueous extract from Opuntia megapotamica fruit pulp promotes osteoblast activityGili, ValeriaLaiuppa, Juan AndrésSantillán, Graciela EdithOSTEOBLASTOPUNTIAPOSTMENOPAUSAL OSTEOPOROSISOSTEOPENIAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Objetive: Osteoporosis and osteopenia are age-related chronic diseases with increased morbidity rates among postmenopausal women. Natural productsinvestigation for prevention and treatment of these conditions is growing, due, in part, to the fact that they allow longer exposure times and minor secondary complications than synthetic drugs. Opuntia plants (Cactaceae) have been shown to possess a broad spectrum of medicinal properties. Opuntia megapotamica is an autochthonous species from Argentina but its action on bone metabolism has not yet been studied. The work aims is to evaluate the action of aqueous extracts of O. megapotamica fruits on the activity and function of osteoblasts in vitro.Methods: Primary osteoblasts were obtained by digestion of the cranial bones of 3-5 day-old Wistar rats. Cells were treated with different dilutions of the aqueous extract (1/50-1/1000), then viability, proliferation, differentiation and mineralization were assessed at the corresponding time points.Results: Dilution of extract 1/100 induced a slight but signifi cant increase in cell viability with respect to control (p< 0.05) whereas the other conditions showed no changes. In addition, dilutions of extract 1/100 and 1/1000 signifi cantly increased cell proliferation at 24 and 48 h with respect to the control (p< 0.05). Culture mineralization, showed a rising trend with respect to the control at 15 days of treatment with 1/1000 extract dilution.Conclusions: The results show that the aqueous extract of O. megapotamica fruit increases the number and improves the biological functions of osteoblasts,suggesting its potential use as an alternative treatment for postmenopausal bone deficit.Fil: Gili, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Laiuppa, Juan Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Santillán, Graciela Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaPeertechz Publications2020-05-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/141503Gili, Valeria; Laiuppa, Juan Andrés; Santillán, Graciela Edith; Aqueous extract from Opuntia megapotamica fruit pulp promotes osteoblast activity; Peertechz Publications; International Journal of Spine Research; 2; 1; 20-5-2020; 37-452692-4692CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.17352/ijsr.000012info:eu-repo/semantics/altIdentifier/url/https://www.peertechzpublications.com/articles/IJSR-2-112.phpinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:49:58Zoai:ri.conicet.gov.ar:11336/141503instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:49:58.949CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Aqueous extract from Opuntia megapotamica fruit pulp promotes osteoblast activity
title Aqueous extract from Opuntia megapotamica fruit pulp promotes osteoblast activity
spellingShingle Aqueous extract from Opuntia megapotamica fruit pulp promotes osteoblast activity
Gili, Valeria
OSTEOBLAST
OPUNTIA
POSTMENOPAUSAL OSTEOPOROSIS
OSTEOPENIA
title_short Aqueous extract from Opuntia megapotamica fruit pulp promotes osteoblast activity
title_full Aqueous extract from Opuntia megapotamica fruit pulp promotes osteoblast activity
title_fullStr Aqueous extract from Opuntia megapotamica fruit pulp promotes osteoblast activity
title_full_unstemmed Aqueous extract from Opuntia megapotamica fruit pulp promotes osteoblast activity
title_sort Aqueous extract from Opuntia megapotamica fruit pulp promotes osteoblast activity
dc.creator.none.fl_str_mv Gili, Valeria
Laiuppa, Juan Andrés
Santillán, Graciela Edith
author Gili, Valeria
author_facet Gili, Valeria
Laiuppa, Juan Andrés
Santillán, Graciela Edith
author_role author
author2 Laiuppa, Juan Andrés
Santillán, Graciela Edith
author2_role author
author
dc.subject.none.fl_str_mv OSTEOBLAST
OPUNTIA
POSTMENOPAUSAL OSTEOPOROSIS
OSTEOPENIA
topic OSTEOBLAST
OPUNTIA
POSTMENOPAUSAL OSTEOPOROSIS
OSTEOPENIA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Objetive: Osteoporosis and osteopenia are age-related chronic diseases with increased morbidity rates among postmenopausal women. Natural productsinvestigation for prevention and treatment of these conditions is growing, due, in part, to the fact that they allow longer exposure times and minor secondary complications than synthetic drugs. Opuntia plants (Cactaceae) have been shown to possess a broad spectrum of medicinal properties. Opuntia megapotamica is an autochthonous species from Argentina but its action on bone metabolism has not yet been studied. The work aims is to evaluate the action of aqueous extracts of O. megapotamica fruits on the activity and function of osteoblasts in vitro.Methods: Primary osteoblasts were obtained by digestion of the cranial bones of 3-5 day-old Wistar rats. Cells were treated with different dilutions of the aqueous extract (1/50-1/1000), then viability, proliferation, differentiation and mineralization were assessed at the corresponding time points.Results: Dilution of extract 1/100 induced a slight but signifi cant increase in cell viability with respect to control (p< 0.05) whereas the other conditions showed no changes. In addition, dilutions of extract 1/100 and 1/1000 signifi cantly increased cell proliferation at 24 and 48 h with respect to the control (p< 0.05). Culture mineralization, showed a rising trend with respect to the control at 15 days of treatment with 1/1000 extract dilution.Conclusions: The results show that the aqueous extract of O. megapotamica fruit increases the number and improves the biological functions of osteoblasts,suggesting its potential use as an alternative treatment for postmenopausal bone deficit.
Fil: Gili, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Laiuppa, Juan Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Santillán, Graciela Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
description Objetive: Osteoporosis and osteopenia are age-related chronic diseases with increased morbidity rates among postmenopausal women. Natural productsinvestigation for prevention and treatment of these conditions is growing, due, in part, to the fact that they allow longer exposure times and minor secondary complications than synthetic drugs. Opuntia plants (Cactaceae) have been shown to possess a broad spectrum of medicinal properties. Opuntia megapotamica is an autochthonous species from Argentina but its action on bone metabolism has not yet been studied. The work aims is to evaluate the action of aqueous extracts of O. megapotamica fruits on the activity and function of osteoblasts in vitro.Methods: Primary osteoblasts were obtained by digestion of the cranial bones of 3-5 day-old Wistar rats. Cells were treated with different dilutions of the aqueous extract (1/50-1/1000), then viability, proliferation, differentiation and mineralization were assessed at the corresponding time points.Results: Dilution of extract 1/100 induced a slight but signifi cant increase in cell viability with respect to control (p< 0.05) whereas the other conditions showed no changes. In addition, dilutions of extract 1/100 and 1/1000 signifi cantly increased cell proliferation at 24 and 48 h with respect to the control (p< 0.05). Culture mineralization, showed a rising trend with respect to the control at 15 days of treatment with 1/1000 extract dilution.Conclusions: The results show that the aqueous extract of O. megapotamica fruit increases the number and improves the biological functions of osteoblasts,suggesting its potential use as an alternative treatment for postmenopausal bone deficit.
publishDate 2020
dc.date.none.fl_str_mv 2020-05-20
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/141503
Gili, Valeria; Laiuppa, Juan Andrés; Santillán, Graciela Edith; Aqueous extract from Opuntia megapotamica fruit pulp promotes osteoblast activity; Peertechz Publications; International Journal of Spine Research; 2; 1; 20-5-2020; 37-45
2692-4692
CONICET Digital
CONICET
url http://hdl.handle.net/11336/141503
identifier_str_mv Gili, Valeria; Laiuppa, Juan Andrés; Santillán, Graciela Edith; Aqueous extract from Opuntia megapotamica fruit pulp promotes osteoblast activity; Peertechz Publications; International Journal of Spine Research; 2; 1; 20-5-2020; 37-45
2692-4692
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.17352/ijsr.000012
info:eu-repo/semantics/altIdentifier/url/https://www.peertechzpublications.com/articles/IJSR-2-112.php
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Peertechz Publications
publisher.none.fl_str_mv Peertechz Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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