Spatio-temporal characterization of Trypanosoma cruzi infection and discrete typing units infecting hosts and vectors from non-domestic foci of Chile
- Autores
- Ihle-Soto, Camila; Costoya, Eduardo; Correa, Juana P.; Bacigalupo, Antonella; Cornejo-Villar, Berenice; Estadella, Viviana; Solari, Aldo; Ortiz, Sylvia; Hernández, Héctor J.; Botto-Mahanid, Carezza; Gorla, David Eladio; Cattan, Pedro E.
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Trypanosoma cruziis a protozoan parasite that is transmitted by triatomine vectors to mam-mals. It is classifiedin six discrete typing units(DTUs).In Chile, domestic vectorial transmission has been interrupted; however, the parasite is maintained in non-domestic foci.The aim of this study was to describe T. cruzi infection and DTU composition in mammals and triatomines from several non-domestic populations of North-Central Chile and to evaluate their spatio-temporal variations. A total of 710 small mammals and 1140 triatomines captured in six localities during two study periods (summer/winter) of the same year were analyzed by conventional PCR to detect kDNA of T. cruzi. Positive samples were DNA blotted and hybridized with specific probes for detection of DTUs TcI, TcII, TcV, and TcVI. Infection status was modeled, and cluster analysis was performed in each locality. We detected 30.1% of overall infection in small mammals and 34.1% in triatomines, with higher rates in synanthropic mammals and in M. spinolai. We identified infecting DTUs in 45 mammals and 110 triatomines, present more commonly as single infections; the most frequent DTU detected was TcI. Differences in infection rates among species, localities and study periods were detected in small mammals, and between triatomine species; temporally, infection presented opposite patterns between mammals and triatomines. Infection clustering was frequent in vectors, and one locality exhibited half of the 21 clusters found. We determined T. cruzi infection in natural host and vector populations simultaneously in a spatially widespread manner during two study periods. All captured species presented T. cruzi infection, showing spatial and temporal variations. Trypanosoma cruzi distribution can be clustered in space and time. These clusters may represent different spatial and temporal risks of transmission.
Fil: Ihle-Soto, Camila. Universidad de Chile; Chile
Fil: Costoya, Eduardo. Universidad de Chile; Chile
Fil: Correa, Juana P.. Universidad de Chile; Chile
Fil: Bacigalupo, Antonella. Universidad de Chile; Chile
Fil: Cornejo-Villar, Berenice. Universidad de Chile; Chile
Fil: Estadella, Viviana. Universidad de Chile; Chile
Fil: Solari, Aldo. Facultad de Medicina de la Universidad de Chile; Chile
Fil: Ortiz, Sylvia. Facultad de Medicina de la Universidad de Chile; Chile
Fil: Hernández, Héctor J.. Universidad de Chile; Chile
Fil: Botto-Mahanid, Carezza. Universidad de Chile; Chile
Fil: Gorla, David Eladio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina
Fil: Cattan, Pedro E.. Universidad de Chile; Chile - Materia
-
TRYPANOSOMA CRUZI
CHAGAS DISEASE
TRIATOMINAE
CHILE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/112323
Ver los metadatos del registro completo
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Spatio-temporal characterization of Trypanosoma cruzi infection and discrete typing units infecting hosts and vectors from non-domestic foci of ChileIhle-Soto, CamilaCostoya, EduardoCorrea, Juana P.Bacigalupo, AntonellaCornejo-Villar, BereniceEstadella, VivianaSolari, AldoOrtiz, SylviaHernández, Héctor J.Botto-Mahanid, CarezzaGorla, David EladioCattan, Pedro E.TRYPANOSOMA CRUZICHAGAS DISEASETRIATOMINAECHILEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Trypanosoma cruziis a protozoan parasite that is transmitted by triatomine vectors to mam-mals. It is classifiedin six discrete typing units(DTUs).In Chile, domestic vectorial transmission has been interrupted; however, the parasite is maintained in non-domestic foci.The aim of this study was to describe T. cruzi infection and DTU composition in mammals and triatomines from several non-domestic populations of North-Central Chile and to evaluate their spatio-temporal variations. A total of 710 small mammals and 1140 triatomines captured in six localities during two study periods (summer/winter) of the same year were analyzed by conventional PCR to detect kDNA of T. cruzi. Positive samples were DNA blotted and hybridized with specific probes for detection of DTUs TcI, TcII, TcV, and TcVI. Infection status was modeled, and cluster analysis was performed in each locality. We detected 30.1% of overall infection in small mammals and 34.1% in triatomines, with higher rates in synanthropic mammals and in M. spinolai. We identified infecting DTUs in 45 mammals and 110 triatomines, present more commonly as single infections; the most frequent DTU detected was TcI. Differences in infection rates among species, localities and study periods were detected in small mammals, and between triatomine species; temporally, infection presented opposite patterns between mammals and triatomines. Infection clustering was frequent in vectors, and one locality exhibited half of the 21 clusters found. We determined T. cruzi infection in natural host and vector populations simultaneously in a spatially widespread manner during two study periods. All captured species presented T. cruzi infection, showing spatial and temporal variations. Trypanosoma cruzi distribution can be clustered in space and time. These clusters may represent different spatial and temporal risks of transmission.Fil: Ihle-Soto, Camila. Universidad de Chile; ChileFil: Costoya, Eduardo. Universidad de Chile; ChileFil: Correa, Juana P.. Universidad de Chile; ChileFil: Bacigalupo, Antonella. Universidad de Chile; ChileFil: Cornejo-Villar, Berenice. Universidad de Chile; ChileFil: Estadella, Viviana. Universidad de Chile; ChileFil: Solari, Aldo. Facultad de Medicina de la Universidad de Chile; ChileFil: Ortiz, Sylvia. Facultad de Medicina de la Universidad de Chile; ChileFil: Hernández, Héctor J.. Universidad de Chile; ChileFil: Botto-Mahanid, Carezza. Universidad de Chile; ChileFil: Gorla, David Eladio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; ArgentinaFil: Cattan, Pedro E.. Universidad de Chile; ChilePublic Library of Science2019-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/112323Ihle-Soto, Camila; Costoya, Eduardo; Correa, Juana P.; Bacigalupo, Antonella; Cornejo-Villar, Berenice; et al.; Spatio-temporal characterization of Trypanosoma cruzi infection and discrete typing units infecting hosts and vectors from non-domestic foci of Chile; Public Library of Science; PLoS Neglected Tropical Diseases; 13; 2; 2-20191935-27271935-2735CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0007170info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0007170info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:36:19Zoai:ri.conicet.gov.ar:11336/112323instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:36:19.804CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Spatio-temporal characterization of Trypanosoma cruzi infection and discrete typing units infecting hosts and vectors from non-domestic foci of Chile |
| title |
Spatio-temporal characterization of Trypanosoma cruzi infection and discrete typing units infecting hosts and vectors from non-domestic foci of Chile |
| spellingShingle |
Spatio-temporal characterization of Trypanosoma cruzi infection and discrete typing units infecting hosts and vectors from non-domestic foci of Chile Ihle-Soto, Camila TRYPANOSOMA CRUZI CHAGAS DISEASE TRIATOMINAE CHILE |
| title_short |
Spatio-temporal characterization of Trypanosoma cruzi infection and discrete typing units infecting hosts and vectors from non-domestic foci of Chile |
| title_full |
Spatio-temporal characterization of Trypanosoma cruzi infection and discrete typing units infecting hosts and vectors from non-domestic foci of Chile |
| title_fullStr |
Spatio-temporal characterization of Trypanosoma cruzi infection and discrete typing units infecting hosts and vectors from non-domestic foci of Chile |
| title_full_unstemmed |
Spatio-temporal characterization of Trypanosoma cruzi infection and discrete typing units infecting hosts and vectors from non-domestic foci of Chile |
| title_sort |
Spatio-temporal characterization of Trypanosoma cruzi infection and discrete typing units infecting hosts and vectors from non-domestic foci of Chile |
| dc.creator.none.fl_str_mv |
Ihle-Soto, Camila Costoya, Eduardo Correa, Juana P. Bacigalupo, Antonella Cornejo-Villar, Berenice Estadella, Viviana Solari, Aldo Ortiz, Sylvia Hernández, Héctor J. Botto-Mahanid, Carezza Gorla, David Eladio Cattan, Pedro E. |
| author |
Ihle-Soto, Camila |
| author_facet |
Ihle-Soto, Camila Costoya, Eduardo Correa, Juana P. Bacigalupo, Antonella Cornejo-Villar, Berenice Estadella, Viviana Solari, Aldo Ortiz, Sylvia Hernández, Héctor J. Botto-Mahanid, Carezza Gorla, David Eladio Cattan, Pedro E. |
| author_role |
author |
| author2 |
Costoya, Eduardo Correa, Juana P. Bacigalupo, Antonella Cornejo-Villar, Berenice Estadella, Viviana Solari, Aldo Ortiz, Sylvia Hernández, Héctor J. Botto-Mahanid, Carezza Gorla, David Eladio Cattan, Pedro E. |
| author2_role |
author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
TRYPANOSOMA CRUZI CHAGAS DISEASE TRIATOMINAE CHILE |
| topic |
TRYPANOSOMA CRUZI CHAGAS DISEASE TRIATOMINAE CHILE |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
Trypanosoma cruziis a protozoan parasite that is transmitted by triatomine vectors to mam-mals. It is classifiedin six discrete typing units(DTUs).In Chile, domestic vectorial transmission has been interrupted; however, the parasite is maintained in non-domestic foci.The aim of this study was to describe T. cruzi infection and DTU composition in mammals and triatomines from several non-domestic populations of North-Central Chile and to evaluate their spatio-temporal variations. A total of 710 small mammals and 1140 triatomines captured in six localities during two study periods (summer/winter) of the same year were analyzed by conventional PCR to detect kDNA of T. cruzi. Positive samples were DNA blotted and hybridized with specific probes for detection of DTUs TcI, TcII, TcV, and TcVI. Infection status was modeled, and cluster analysis was performed in each locality. We detected 30.1% of overall infection in small mammals and 34.1% in triatomines, with higher rates in synanthropic mammals and in M. spinolai. We identified infecting DTUs in 45 mammals and 110 triatomines, present more commonly as single infections; the most frequent DTU detected was TcI. Differences in infection rates among species, localities and study periods were detected in small mammals, and between triatomine species; temporally, infection presented opposite patterns between mammals and triatomines. Infection clustering was frequent in vectors, and one locality exhibited half of the 21 clusters found. We determined T. cruzi infection in natural host and vector populations simultaneously in a spatially widespread manner during two study periods. All captured species presented T. cruzi infection, showing spatial and temporal variations. Trypanosoma cruzi distribution can be clustered in space and time. These clusters may represent different spatial and temporal risks of transmission. Fil: Ihle-Soto, Camila. Universidad de Chile; Chile Fil: Costoya, Eduardo. Universidad de Chile; Chile Fil: Correa, Juana P.. Universidad de Chile; Chile Fil: Bacigalupo, Antonella. Universidad de Chile; Chile Fil: Cornejo-Villar, Berenice. Universidad de Chile; Chile Fil: Estadella, Viviana. Universidad de Chile; Chile Fil: Solari, Aldo. Facultad de Medicina de la Universidad de Chile; Chile Fil: Ortiz, Sylvia. Facultad de Medicina de la Universidad de Chile; Chile Fil: Hernández, Héctor J.. Universidad de Chile; Chile Fil: Botto-Mahanid, Carezza. Universidad de Chile; Chile Fil: Gorla, David Eladio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina Fil: Cattan, Pedro E.. Universidad de Chile; Chile |
| description |
Trypanosoma cruziis a protozoan parasite that is transmitted by triatomine vectors to mam-mals. It is classifiedin six discrete typing units(DTUs).In Chile, domestic vectorial transmission has been interrupted; however, the parasite is maintained in non-domestic foci.The aim of this study was to describe T. cruzi infection and DTU composition in mammals and triatomines from several non-domestic populations of North-Central Chile and to evaluate their spatio-temporal variations. A total of 710 small mammals and 1140 triatomines captured in six localities during two study periods (summer/winter) of the same year were analyzed by conventional PCR to detect kDNA of T. cruzi. Positive samples were DNA blotted and hybridized with specific probes for detection of DTUs TcI, TcII, TcV, and TcVI. Infection status was modeled, and cluster analysis was performed in each locality. We detected 30.1% of overall infection in small mammals and 34.1% in triatomines, with higher rates in synanthropic mammals and in M. spinolai. We identified infecting DTUs in 45 mammals and 110 triatomines, present more commonly as single infections; the most frequent DTU detected was TcI. Differences in infection rates among species, localities and study periods were detected in small mammals, and between triatomine species; temporally, infection presented opposite patterns between mammals and triatomines. Infection clustering was frequent in vectors, and one locality exhibited half of the 21 clusters found. We determined T. cruzi infection in natural host and vector populations simultaneously in a spatially widespread manner during two study periods. All captured species presented T. cruzi infection, showing spatial and temporal variations. Trypanosoma cruzi distribution can be clustered in space and time. These clusters may represent different spatial and temporal risks of transmission. |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2019-02 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://hdl.handle.net/11336/112323 Ihle-Soto, Camila; Costoya, Eduardo; Correa, Juana P.; Bacigalupo, Antonella; Cornejo-Villar, Berenice; et al.; Spatio-temporal characterization of Trypanosoma cruzi infection and discrete typing units infecting hosts and vectors from non-domestic foci of Chile; Public Library of Science; PLoS Neglected Tropical Diseases; 13; 2; 2-2019 1935-2727 1935-2735 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/112323 |
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Ihle-Soto, Camila; Costoya, Eduardo; Correa, Juana P.; Bacigalupo, Antonella; Cornejo-Villar, Berenice; et al.; Spatio-temporal characterization of Trypanosoma cruzi infection and discrete typing units infecting hosts and vectors from non-domestic foci of Chile; Public Library of Science; PLoS Neglected Tropical Diseases; 13; 2; 2-2019 1935-2727 1935-2735 CONICET Digital CONICET |
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eng |
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eng |
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