Antibacterial activity of gelatin/copper (II)-exchanged montmorillonite films

Autores
Martucci, Josefa Fabiana; Ruseckaite, Roxana Alejandra
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cu (II) - exchanged montmorillonite (Cu2+MMt) was prepared, characterized and introduced into a bovine gelatin (Ge) matrix via a dissolution-intercalation method to get antibacterial nanocomposite films. The maximum amount of exchanged cation did not exceed the cation exchange capacity of the pristine montmorillonite (Na+MMt), as assessed by energy dispersive X-ray (EDX) spectroscopy. Cu2+MMt showed antibacterial activity in vitro against Escherichia coli O157:H7 (Gram-negative) and Listeria monocytogenes (Gram-positive) as revealed by the agar disc-diffusion assay. The dispersion of clays in Ge films was monitored by X-ray diffraction (XRD) and Scanning Electron Microscopy (SEM). Blending gelatin with 5% w/w of clay increased the tensile strength of the nanocomposite films in around 280% while the elongation at break and the water vapor permeability decreased in about 42 and 30%, respectively, regardless of the cation in clay. The Ge/Cu2+MMt film exhibited antibacterial effectiveness against both pathogens tested under the same conditions, demonstrating a stronger effect on L. monocytogenes than on E. coli O157:H7, since the cell wall of the latter differs significantly and such difference could influence their vulnerability and response to the active films. Therefore, the incorporation of low clay levels as a vehicle for copper ions into gelatin matrix has demonstrated to be a good method for developing functional materials that can be potentially applied to the design of food contact items.
Fil: Martucci, Josefa Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Ruseckaite, Roxana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Materia
Bovine gelatin
Cupric ions
Montmorillonite
Nanocomposite
Active film
Antimicrobial activity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/32561
Acceder
id CONICETDig_406785e70734ae58baa9895394813460
oai_identifier_str oai:ri.conicet.gov.ar:11336/32561
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antibacterial activity of gelatin/copper (II)-exchanged montmorillonite filmsMartucci, Josefa FabianaRuseckaite, Roxana AlejandraBovine gelatinCupric ionsMontmorilloniteNanocompositeActive filmAntimicrobial activityhttps://purl.org/becyt/ford/2.5https://purl.org/becyt/ford/2https://purl.org/becyt/ford/2.5https://purl.org/becyt/ford/2Cu (II) - exchanged montmorillonite (Cu2+MMt) was prepared, characterized and introduced into a bovine gelatin (Ge) matrix via a dissolution-intercalation method to get antibacterial nanocomposite films. The maximum amount of exchanged cation did not exceed the cation exchange capacity of the pristine montmorillonite (Na+MMt), as assessed by energy dispersive X-ray (EDX) spectroscopy. Cu2+MMt showed antibacterial activity in vitro against Escherichia coli O157:H7 (Gram-negative) and Listeria monocytogenes (Gram-positive) as revealed by the agar disc-diffusion assay. The dispersion of clays in Ge films was monitored by X-ray diffraction (XRD) and Scanning Electron Microscopy (SEM). Blending gelatin with 5% w/w of clay increased the tensile strength of the nanocomposite films in around 280% while the elongation at break and the water vapor permeability decreased in about 42 and 30%, respectively, regardless of the cation in clay. The Ge/Cu2+MMt film exhibited antibacterial effectiveness against both pathogens tested under the same conditions, demonstrating a stronger effect on L. monocytogenes than on E. coli O157:H7, since the cell wall of the latter differs significantly and such difference could influence their vulnerability and response to the active films. Therefore, the incorporation of low clay levels as a vehicle for copper ions into gelatin matrix has demonstrated to be a good method for developing functional materials that can be potentially applied to the design of food contact items.Fil: Martucci, Josefa Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Ruseckaite, Roxana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaElsevier2016-10-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/32561Martucci, Josefa Fabiana; Ruseckaite, Roxana Alejandra; Antibacterial activity of gelatin/copper (II)-exchanged montmorillonite films; Elsevier; Food Hydrocolloids; 64; 21-10-2016; 70-770268-005XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0268005X16306233info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodhyd.2016.10.030info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:56:44Zoai:ri.conicet.gov.ar:11336/32561instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:56:44.291CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antibacterial activity of gelatin/copper (II)-exchanged montmorillonite films
title Antibacterial activity of gelatin/copper (II)-exchanged montmorillonite films
spellingShingle Antibacterial activity of gelatin/copper (II)-exchanged montmorillonite films
Martucci, Josefa Fabiana
Bovine gelatin
Cupric ions
Montmorillonite
Nanocomposite
Active film
Antimicrobial activity
title_short Antibacterial activity of gelatin/copper (II)-exchanged montmorillonite films
title_full Antibacterial activity of gelatin/copper (II)-exchanged montmorillonite films
title_fullStr Antibacterial activity of gelatin/copper (II)-exchanged montmorillonite films
title_full_unstemmed Antibacterial activity of gelatin/copper (II)-exchanged montmorillonite films
title_sort Antibacterial activity of gelatin/copper (II)-exchanged montmorillonite films
dc.creator.none.fl_str_mv Martucci, Josefa Fabiana
Ruseckaite, Roxana Alejandra
author Martucci, Josefa Fabiana
author_facet Martucci, Josefa Fabiana
Ruseckaite, Roxana Alejandra
author_role author
author2 Ruseckaite, Roxana Alejandra
author2_role author
dc.subject.none.fl_str_mv Bovine gelatin
Cupric ions
Montmorillonite
Nanocomposite
Active film
Antimicrobial activity
topic Bovine gelatin
Cupric ions
Montmorillonite
Nanocomposite
Active film
Antimicrobial activity
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.5
https://purl.org/becyt/ford/2
https://purl.org/becyt/ford/2.5
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Cu (II) - exchanged montmorillonite (Cu2+MMt) was prepared, characterized and introduced into a bovine gelatin (Ge) matrix via a dissolution-intercalation method to get antibacterial nanocomposite films. The maximum amount of exchanged cation did not exceed the cation exchange capacity of the pristine montmorillonite (Na+MMt), as assessed by energy dispersive X-ray (EDX) spectroscopy. Cu2+MMt showed antibacterial activity in vitro against Escherichia coli O157:H7 (Gram-negative) and Listeria monocytogenes (Gram-positive) as revealed by the agar disc-diffusion assay. The dispersion of clays in Ge films was monitored by X-ray diffraction (XRD) and Scanning Electron Microscopy (SEM). Blending gelatin with 5% w/w of clay increased the tensile strength of the nanocomposite films in around 280% while the elongation at break and the water vapor permeability decreased in about 42 and 30%, respectively, regardless of the cation in clay. The Ge/Cu2+MMt film exhibited antibacterial effectiveness against both pathogens tested under the same conditions, demonstrating a stronger effect on L. monocytogenes than on E. coli O157:H7, since the cell wall of the latter differs significantly and such difference could influence their vulnerability and response to the active films. Therefore, the incorporation of low clay levels as a vehicle for copper ions into gelatin matrix has demonstrated to be a good method for developing functional materials that can be potentially applied to the design of food contact items.
Fil: Martucci, Josefa Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Ruseckaite, Roxana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
description Cu (II) - exchanged montmorillonite (Cu2+MMt) was prepared, characterized and introduced into a bovine gelatin (Ge) matrix via a dissolution-intercalation method to get antibacterial nanocomposite films. The maximum amount of exchanged cation did not exceed the cation exchange capacity of the pristine montmorillonite (Na+MMt), as assessed by energy dispersive X-ray (EDX) spectroscopy. Cu2+MMt showed antibacterial activity in vitro against Escherichia coli O157:H7 (Gram-negative) and Listeria monocytogenes (Gram-positive) as revealed by the agar disc-diffusion assay. The dispersion of clays in Ge films was monitored by X-ray diffraction (XRD) and Scanning Electron Microscopy (SEM). Blending gelatin with 5% w/w of clay increased the tensile strength of the nanocomposite films in around 280% while the elongation at break and the water vapor permeability decreased in about 42 and 30%, respectively, regardless of the cation in clay. The Ge/Cu2+MMt film exhibited antibacterial effectiveness against both pathogens tested under the same conditions, demonstrating a stronger effect on L. monocytogenes than on E. coli O157:H7, since the cell wall of the latter differs significantly and such difference could influence their vulnerability and response to the active films. Therefore, the incorporation of low clay levels as a vehicle for copper ions into gelatin matrix has demonstrated to be a good method for developing functional materials that can be potentially applied to the design of food contact items.
publishDate 2016
dc.date.none.fl_str_mv 2016-10-21
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/32561
Martucci, Josefa Fabiana; Ruseckaite, Roxana Alejandra; Antibacterial activity of gelatin/copper (II)-exchanged montmorillonite films; Elsevier; Food Hydrocolloids; 64; 21-10-2016; 70-77
0268-005X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/32561
identifier_str_mv Martucci, Josefa Fabiana; Ruseckaite, Roxana Alejandra; Antibacterial activity of gelatin/copper (II)-exchanged montmorillonite films; Elsevier; Food Hydrocolloids; 64; 21-10-2016; 70-77
0268-005X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0268005X16306233
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodhyd.2016.10.030
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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