UGA4 gene encoding the γ-aminobutyric acid permease in Saccharomyces cerevisiae is an acid-expressed gene

Autores
Bermudez Moretti, Mariana; Batlle, Alcira María del C.; Correa Garcia, Susana Raquel
Año de publicación
2001
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background and aims: biological processes in all organisms are controlled by environmental conditions, however, information concerning the molecular responses to external pH is scarce. In this work we studied the pH response of UGA4 gene encoding -aminolevulinic acid and -aminobutyric acid permease in Saccharomyces cere isiae. Methods: we analyzed the effect of pH on the expression of UGA4 gene measuring -galactosidase activity in cells carrying a UGA4::lacZ fusion gene. Results: results indicate that UGA4 expression is higher at acidic pH. The expression of UGA3 and UGA35 genes, which encode two positive transcription factors, is not regulated by external pH, while the expression of UGA43 gene encoding a repressor of UGA4 transcription is dependent on pH. Using a strain lacking Uga43p we clearly showed that the effect of ambient pH on UGA4 expression is not a secondary effect of the pH regulation on UGA43. We have also demonstrated that the effect of pH can only be detected when UGA4 gene is not subject to a strong repression by Uga43p nor to GABA induction. Conclusion: here, we demonstrate that UGA4 is an acid-expressed gene. This regulation is probably mediated by Rim101p through the consensus site 5-GCCARG-3 at 237 bp preceding the UGA4 coding sequence.
Fil: Bermudez Moretti, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina
Fil: Batlle, Alcira María del C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina
Fil: Correa Garcia, Susana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Materia
UGA4 gene
Saccharomyces cerevisiae
Ambient pH
Transcriptional regulation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/102043
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/102043
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling UGA4 gene encoding the γ-aminobutyric acid permease in Saccharomyces cerevisiae is an acid-expressed geneBermudez Moretti, MarianaBatlle, Alcira María del C.Correa Garcia, Susana RaquelUGA4 geneSaccharomyces cerevisiaeAmbient pHTranscriptional regulationhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Background and aims: biological processes in all organisms are controlled by environmental conditions, however, information concerning the molecular responses to external pH is scarce. In this work we studied the pH response of UGA4 gene encoding -aminolevulinic acid and -aminobutyric acid permease in Saccharomyces cere isiae. Methods: we analyzed the effect of pH on the expression of UGA4 gene measuring -galactosidase activity in cells carrying a UGA4::lacZ fusion gene. Results: results indicate that UGA4 expression is higher at acidic pH. The expression of UGA3 and UGA35 genes, which encode two positive transcription factors, is not regulated by external pH, while the expression of UGA43 gene encoding a repressor of UGA4 transcription is dependent on pH. Using a strain lacking Uga43p we clearly showed that the effect of ambient pH on UGA4 expression is not a secondary effect of the pH regulation on UGA43. We have also demonstrated that the effect of pH can only be detected when UGA4 gene is not subject to a strong repression by Uga43p nor to GABA induction. Conclusion: here, we demonstrate that UGA4 is an acid-expressed gene. This regulation is probably mediated by Rim101p through the consensus site 5-GCCARG-3 at 237 bp preceding the UGA4 coding sequence.Fil: Bermudez Moretti, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; ArgentinaFil: Batlle, Alcira María del C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; ArgentinaFil: Correa Garcia, Susana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaPergamon-Elsevier Science Ltd2001-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/102043Bermudez Moretti, Mariana; Batlle, Alcira María del C.; Correa Garcia, Susana Raquel; UGA4 gene encoding the γ-aminobutyric acid permease in Saccharomyces cerevisiae is an acid-expressed gene; Pergamon-Elsevier Science Ltd; International Journal of Biochemistry and Cellular Biology; 33; 12; 12-2001; 1202-12071357-2725CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S1357272501000851info:eu-repo/semantics/altIdentifier/doi/10.1016/S1357-2725(01)00085-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:07:35Zoai:ri.conicet.gov.ar:11336/102043instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:07:35.571CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv UGA4 gene encoding the γ-aminobutyric acid permease in Saccharomyces cerevisiae is an acid-expressed gene
title UGA4 gene encoding the γ-aminobutyric acid permease in Saccharomyces cerevisiae is an acid-expressed gene
spellingShingle UGA4 gene encoding the γ-aminobutyric acid permease in Saccharomyces cerevisiae is an acid-expressed gene
Bermudez Moretti, Mariana
UGA4 gene
Saccharomyces cerevisiae
Ambient pH
Transcriptional regulation
title_short UGA4 gene encoding the γ-aminobutyric acid permease in Saccharomyces cerevisiae is an acid-expressed gene
title_full UGA4 gene encoding the γ-aminobutyric acid permease in Saccharomyces cerevisiae is an acid-expressed gene
title_fullStr UGA4 gene encoding the γ-aminobutyric acid permease in Saccharomyces cerevisiae is an acid-expressed gene
title_full_unstemmed UGA4 gene encoding the γ-aminobutyric acid permease in Saccharomyces cerevisiae is an acid-expressed gene
title_sort UGA4 gene encoding the γ-aminobutyric acid permease in Saccharomyces cerevisiae is an acid-expressed gene
dc.creator.none.fl_str_mv Bermudez Moretti, Mariana
Batlle, Alcira María del C.
Correa Garcia, Susana Raquel
author Bermudez Moretti, Mariana
author_facet Bermudez Moretti, Mariana
Batlle, Alcira María del C.
Correa Garcia, Susana Raquel
author_role author
author2 Batlle, Alcira María del C.
Correa Garcia, Susana Raquel
author2_role author
author
dc.subject.none.fl_str_mv UGA4 gene
Saccharomyces cerevisiae
Ambient pH
Transcriptional regulation
topic UGA4 gene
Saccharomyces cerevisiae
Ambient pH
Transcriptional regulation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background and aims: biological processes in all organisms are controlled by environmental conditions, however, information concerning the molecular responses to external pH is scarce. In this work we studied the pH response of UGA4 gene encoding -aminolevulinic acid and -aminobutyric acid permease in Saccharomyces cere isiae. Methods: we analyzed the effect of pH on the expression of UGA4 gene measuring -galactosidase activity in cells carrying a UGA4::lacZ fusion gene. Results: results indicate that UGA4 expression is higher at acidic pH. The expression of UGA3 and UGA35 genes, which encode two positive transcription factors, is not regulated by external pH, while the expression of UGA43 gene encoding a repressor of UGA4 transcription is dependent on pH. Using a strain lacking Uga43p we clearly showed that the effect of ambient pH on UGA4 expression is not a secondary effect of the pH regulation on UGA43. We have also demonstrated that the effect of pH can only be detected when UGA4 gene is not subject to a strong repression by Uga43p nor to GABA induction. Conclusion: here, we demonstrate that UGA4 is an acid-expressed gene. This regulation is probably mediated by Rim101p through the consensus site 5-GCCARG-3 at 237 bp preceding the UGA4 coding sequence.
Fil: Bermudez Moretti, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina
Fil: Batlle, Alcira María del C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina
Fil: Correa Garcia, Susana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
description Background and aims: biological processes in all organisms are controlled by environmental conditions, however, information concerning the molecular responses to external pH is scarce. In this work we studied the pH response of UGA4 gene encoding -aminolevulinic acid and -aminobutyric acid permease in Saccharomyces cere isiae. Methods: we analyzed the effect of pH on the expression of UGA4 gene measuring -galactosidase activity in cells carrying a UGA4::lacZ fusion gene. Results: results indicate that UGA4 expression is higher at acidic pH. The expression of UGA3 and UGA35 genes, which encode two positive transcription factors, is not regulated by external pH, while the expression of UGA43 gene encoding a repressor of UGA4 transcription is dependent on pH. Using a strain lacking Uga43p we clearly showed that the effect of ambient pH on UGA4 expression is not a secondary effect of the pH regulation on UGA43. We have also demonstrated that the effect of pH can only be detected when UGA4 gene is not subject to a strong repression by Uga43p nor to GABA induction. Conclusion: here, we demonstrate that UGA4 is an acid-expressed gene. This regulation is probably mediated by Rim101p through the consensus site 5-GCCARG-3 at 237 bp preceding the UGA4 coding sequence.
publishDate 2001
dc.date.none.fl_str_mv 2001-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/102043
Bermudez Moretti, Mariana; Batlle, Alcira María del C.; Correa Garcia, Susana Raquel; UGA4 gene encoding the γ-aminobutyric acid permease in Saccharomyces cerevisiae is an acid-expressed gene; Pergamon-Elsevier Science Ltd; International Journal of Biochemistry and Cellular Biology; 33; 12; 12-2001; 1202-1207
1357-2725
CONICET Digital
CONICET
url http://hdl.handle.net/11336/102043
identifier_str_mv Bermudez Moretti, Mariana; Batlle, Alcira María del C.; Correa Garcia, Susana Raquel; UGA4 gene encoding the γ-aminobutyric acid permease in Saccharomyces cerevisiae is an acid-expressed gene; Pergamon-Elsevier Science Ltd; International Journal of Biochemistry and Cellular Biology; 33; 12; 12-2001; 1202-1207
1357-2725
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S1357272501000851
info:eu-repo/semantics/altIdentifier/doi/10.1016/S1357-2725(01)00085-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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