Constitutive expression of the UGA4 gene in Saccharomyces cerevisiae depends on two positive-acting proteins, Uga3p and Uga35p

• Autores: Garcia, S.C.; Moretti, M.B.; Batlle, A.
• Año de publicación: 2000
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: FEMS Microbiol. Lett. 2000;184(2):219-224
• Materia: γ-Aminobutyric acid; δ-Aminolevulinic acid; Transcriptional factor; Transport regulation; Uga4 permease; Yeast; 4 aminobutyric acid; aminolevulinic acid; permease; article; controlled study; gene expression regulation; nonhuman; priority journal; promoter region; protein transport; Saccharomyces cerevisiae; transcription regulation; Alanine; Aminolevulinic Acid; beta-Galactosidase; DNA-Binding Proteins; Fungal Proteins; GABA Plasma Membrane Transport Proteins; gamma-Aminobutyric Acid; Gene Expression Regulation, Fungal; Membrane Transport Proteins; Organic Anion Transporters; Promoter Regions (Genetics); Recombinant Fusion Proteins; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Transcription Factors; Saccharomyces cerevisiae
• Institución: Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales

The first specific precursor of porphyrin biosynthesis is δ-aminolevulinic acid. δ-Aminolevulinic acid enters Saccharomyces cerevisiae cells through the γ-aminobutyric acid specific permease Uga4p. It was described that this permease is inducible by γ-aminobutyric acid and its regulation involves several specific and pleiotropic transcriptional factors. However, some studies showed that under certain growth conditions the synthesis of Uga4p was not dependent on the presence of γ-aminobutyric acid. To study the effect of the trans-acting factors Uga43p, Uga3p, Uga35p, Ure2p and Gln3p on the expression of UGA4, we measured γ-aminobutyric acid and δ-aminolevulinic acid uptake in yeast mutant cells, lacking one of these regulatory factors, grown under different conditions. Experiments analyzing the UGA4 promoter using a fusion construction UGA4::lacZ were also carried out. The results show that the constitutive expression of the UGA4 gene found in cells under certain growth conditions depends on the presence of Uga3p and Uga35p. In contrast, Gln3p and Ure2p do not seem to have any effect on this constitutive mechanism. Copyright (C) 2000 Federation of European Microbiological Societies.
Fil:Batlle, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.