Uga3 and Uga35/Dal81 transcription factors regulate UGA4 transcription in response to γ-Aminobutyric acid and Leucine

• Autores: Cardillo, S.B.; Moretti, M.B.; García, S.C.
• Año de publicación: 2010
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Eukaryotic Cell 2010;9(8):1262-1271
• Materia: 4 aminobutyric acid; 4 aminobutyric acid carrier; DAL81 protein, S cerevisiae; DNA binding protein; leucine; Saccharomyces cerevisiae protein; transcription factor; UGA3 protein, S cerevisiae; UGA4 protein, S cerevisiae; 4 aminobutyric acid; 4 aminobutyric acid carrier; DNA binding protein; leucine; protein binding; Saccharomyces cerevisiae protein; transcription factor; article; biological model; drug effect; fungal gene; gene expression regulation; genetic transcription; genetics; metabolism; mutation; promoter region; protein binding; Saccharomyces cerevisiae; drug effects; genetic transcription; genetics; metabolism; Saccharomyces cerevisiae; DNA-Binding Proteins; GABA Plasma Membrane Transport Proteins; gamma-Aminobutyric Acid; Gene Expression Regulation, Fungal; Genes, Fungal; Leucine; Models, Genetic; Mutation; Promoter Regions, Genetic; Protein Binding; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Transcription Factors; Transcription, Genetic; Saccharomyces cerevisiae; DNA-Binding Proteins; GABA Plasma Membrane Transport Proteins; gamma-Aminobutyric Acid; Gene Expression Regulation, Fungal; Genes, Fungal; Leucine; Models, Genetic; Mutation; Promoter Regions, Genetic; Protein Binding; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Transcription Factors; Transcription, Genetic
• Institución: Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales

The Saccharomyces cerevisiae UGA4 gene encodes a permease capable of importing γ-aminobutyric acid (GABA) and δ-aminolevulinic acid (ALA) into the cell. GABA-dependent induction of this permease requires at least two positive-acting proteins, the specific factor Uga3 and the pleiotropic factor Uga35/Dal81. UGA4 is subjected to a very complex regulation, and its induction is affected by the presence of extracellular amino acids; this effect is mediated by the plasma membrane amino acid sensor SPS. Our results show that leucine affects UGA4 induction and that the SPS sensor and the downstream effectors Stp1 and Stp2 participate in this regulation. Moreover, we found that the Uga3 and Uga35/Dal81 transcription factors bind to the UGA4 promoter in a GABA-dependent manner and that this binding is impaired by the presence of leucine. We also found that the Leu3 transcription factor negatively regulates UGA4 transcription, although this seems to be through an indirect mechanism. © 2010, American Society for Microbiology.
Fil:Cardillo, S.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.