Interleukin-1β regulates CFTR expression in human intestinal T84 cells

Autores
Cafferata, Eduardo Gustavo Alfredo; González Guerrico, Anatilde M.; Giordano, Luciana; Pivetta, Omar Hilario; Santa Coloma, Tomás Antonio
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cystic fibrosis is an autosomal recessive genetic disease, produced by a mutation in the CFTR gene that impairs its function as a chloride channel. In this work, we have examined the effects of interleukin-1β (IL-1β) on the expression of CFTR in human colonic T84 cells. Treatment of T84 cells with IL-1β (0.25 ng/ml) for 4 h resulted in an increased CFTR expression (mRNA and protein). However, higher doses of IL-1β (1 ng/ml and over) produced inhibition of CFTR mRNA and protein expression. The protein kinase C (PKC) inhibitors H7 (50 μM) and GF109203X (1 μM) inhibited the stimulatory effect of IL-1β. Similar effects were seen in the presence of the protein tyrosine kinase (PTK) inhibitors genistein (60 μM) and herbymicin A (2 μM). These results suggest that some PKC isoform(s) and at least a PTK might be involved in the CFTR up-regulation induced by IL-1β. The repression of CFTR up-regulation by cycloheximide (35.5 μM) suggests the participation of a de novo synthesized protein. Results obtained by using the RNA polymerase II inhibitor DRB (78 μM), suggest that the increased mRNA levels seen after IL-1β treatment are not due to an increased stability of the message. We conclude that the CFTR mRNA and protein levels are modulated by IL-1β, this cytokine being the first extracellular protein known to up-regulate CFTR gene expression.
Fil: Cafferata, Eduardo Gustavo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán". Centro Nacional de Genética Médica; Argentina
Fil: González Guerrico, Anatilde M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Giordano, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Pivetta, Omar Hilario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán". Centro Nacional de Genética Médica; Argentina
Fil: Santa Coloma, Tomás Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Materia
Cftr
Cystic Fibrosis
Interleukin-1Β
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/71797
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/71797
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Interleukin-1β regulates CFTR expression in human intestinal T84 cellsCafferata, Eduardo Gustavo AlfredoGonzález Guerrico, Anatilde M.Giordano, LucianaPivetta, Omar HilarioSanta Coloma, Tomás AntonioCftrCystic FibrosisInterleukin-1Βhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Cystic fibrosis is an autosomal recessive genetic disease, produced by a mutation in the CFTR gene that impairs its function as a chloride channel. In this work, we have examined the effects of interleukin-1β (IL-1β) on the expression of CFTR in human colonic T84 cells. Treatment of T84 cells with IL-1β (0.25 ng/ml) for 4 h resulted in an increased CFTR expression (mRNA and protein). However, higher doses of IL-1β (1 ng/ml and over) produced inhibition of CFTR mRNA and protein expression. The protein kinase C (PKC) inhibitors H7 (50 μM) and GF109203X (1 μM) inhibited the stimulatory effect of IL-1β. Similar effects were seen in the presence of the protein tyrosine kinase (PTK) inhibitors genistein (60 μM) and herbymicin A (2 μM). These results suggest that some PKC isoform(s) and at least a PTK might be involved in the CFTR up-regulation induced by IL-1β. The repression of CFTR up-regulation by cycloheximide (35.5 μM) suggests the participation of a de novo synthesized protein. Results obtained by using the RNA polymerase II inhibitor DRB (78 μM), suggest that the increased mRNA levels seen after IL-1β treatment are not due to an increased stability of the message. We conclude that the CFTR mRNA and protein levels are modulated by IL-1β, this cytokine being the first extracellular protein known to up-regulate CFTR gene expression.Fil: Cafferata, Eduardo Gustavo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán". Centro Nacional de Genética Médica; ArgentinaFil: González Guerrico, Anatilde M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Giordano, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Pivetta, Omar Hilario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán". Centro Nacional de Genética Médica; ArgentinaFil: Santa Coloma, Tomás Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaElsevier Science2000-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/71797Cafferata, Eduardo Gustavo Alfredo; González Guerrico, Anatilde M.; Giordano, Luciana; Pivetta, Omar Hilario; Santa Coloma, Tomás Antonio; Interleukin-1β regulates CFTR expression in human intestinal T84 cells; Elsevier Science; Biochimica et Biophysica Acta - Molecular Basis of Disease; 1500; 2; 2-2000; 241-2480925-4439CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/S0925-4439(99)00105-2info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0925443999001052info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:12:35Zoai:ri.conicet.gov.ar:11336/71797instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:12:35.867CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Interleukin-1β regulates CFTR expression in human intestinal T84 cells
title Interleukin-1β regulates CFTR expression in human intestinal T84 cells
spellingShingle Interleukin-1β regulates CFTR expression in human intestinal T84 cells
Cafferata, Eduardo Gustavo Alfredo
Cftr
Cystic Fibrosis
Interleukin-1Β
title_short Interleukin-1β regulates CFTR expression in human intestinal T84 cells
title_full Interleukin-1β regulates CFTR expression in human intestinal T84 cells
title_fullStr Interleukin-1β regulates CFTR expression in human intestinal T84 cells
title_full_unstemmed Interleukin-1β regulates CFTR expression in human intestinal T84 cells
title_sort Interleukin-1β regulates CFTR expression in human intestinal T84 cells
dc.creator.none.fl_str_mv Cafferata, Eduardo Gustavo Alfredo
González Guerrico, Anatilde M.
Giordano, Luciana
Pivetta, Omar Hilario
Santa Coloma, Tomás Antonio
author Cafferata, Eduardo Gustavo Alfredo
author_facet Cafferata, Eduardo Gustavo Alfredo
González Guerrico, Anatilde M.
Giordano, Luciana
Pivetta, Omar Hilario
Santa Coloma, Tomás Antonio
author_role author
author2 González Guerrico, Anatilde M.
Giordano, Luciana
Pivetta, Omar Hilario
Santa Coloma, Tomás Antonio
author2_role author
author
author
author
dc.subject.none.fl_str_mv Cftr
Cystic Fibrosis
Interleukin-1Β
topic Cftr
Cystic Fibrosis
Interleukin-1Β
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Cystic fibrosis is an autosomal recessive genetic disease, produced by a mutation in the CFTR gene that impairs its function as a chloride channel. In this work, we have examined the effects of interleukin-1β (IL-1β) on the expression of CFTR in human colonic T84 cells. Treatment of T84 cells with IL-1β (0.25 ng/ml) for 4 h resulted in an increased CFTR expression (mRNA and protein). However, higher doses of IL-1β (1 ng/ml and over) produced inhibition of CFTR mRNA and protein expression. The protein kinase C (PKC) inhibitors H7 (50 μM) and GF109203X (1 μM) inhibited the stimulatory effect of IL-1β. Similar effects were seen in the presence of the protein tyrosine kinase (PTK) inhibitors genistein (60 μM) and herbymicin A (2 μM). These results suggest that some PKC isoform(s) and at least a PTK might be involved in the CFTR up-regulation induced by IL-1β. The repression of CFTR up-regulation by cycloheximide (35.5 μM) suggests the participation of a de novo synthesized protein. Results obtained by using the RNA polymerase II inhibitor DRB (78 μM), suggest that the increased mRNA levels seen after IL-1β treatment are not due to an increased stability of the message. We conclude that the CFTR mRNA and protein levels are modulated by IL-1β, this cytokine being the first extracellular protein known to up-regulate CFTR gene expression.
Fil: Cafferata, Eduardo Gustavo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán". Centro Nacional de Genética Médica; Argentina
Fil: González Guerrico, Anatilde M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Giordano, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Pivetta, Omar Hilario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán". Centro Nacional de Genética Médica; Argentina
Fil: Santa Coloma, Tomás Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
description Cystic fibrosis is an autosomal recessive genetic disease, produced by a mutation in the CFTR gene that impairs its function as a chloride channel. In this work, we have examined the effects of interleukin-1β (IL-1β) on the expression of CFTR in human colonic T84 cells. Treatment of T84 cells with IL-1β (0.25 ng/ml) for 4 h resulted in an increased CFTR expression (mRNA and protein). However, higher doses of IL-1β (1 ng/ml and over) produced inhibition of CFTR mRNA and protein expression. The protein kinase C (PKC) inhibitors H7 (50 μM) and GF109203X (1 μM) inhibited the stimulatory effect of IL-1β. Similar effects were seen in the presence of the protein tyrosine kinase (PTK) inhibitors genistein (60 μM) and herbymicin A (2 μM). These results suggest that some PKC isoform(s) and at least a PTK might be involved in the CFTR up-regulation induced by IL-1β. The repression of CFTR up-regulation by cycloheximide (35.5 μM) suggests the participation of a de novo synthesized protein. Results obtained by using the RNA polymerase II inhibitor DRB (78 μM), suggest that the increased mRNA levels seen after IL-1β treatment are not due to an increased stability of the message. We conclude that the CFTR mRNA and protein levels are modulated by IL-1β, this cytokine being the first extracellular protein known to up-regulate CFTR gene expression.
publishDate 2000
dc.date.none.fl_str_mv 2000-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/71797
Cafferata, Eduardo Gustavo Alfredo; González Guerrico, Anatilde M.; Giordano, Luciana; Pivetta, Omar Hilario; Santa Coloma, Tomás Antonio; Interleukin-1β regulates CFTR expression in human intestinal T84 cells; Elsevier Science; Biochimica et Biophysica Acta - Molecular Basis of Disease; 1500; 2; 2-2000; 241-248
0925-4439
CONICET Digital
CONICET
url http://hdl.handle.net/11336/71797
identifier_str_mv Cafferata, Eduardo Gustavo Alfredo; González Guerrico, Anatilde M.; Giordano, Luciana; Pivetta, Omar Hilario; Santa Coloma, Tomás Antonio; Interleukin-1β regulates CFTR expression in human intestinal T84 cells; Elsevier Science; Biochimica et Biophysica Acta - Molecular Basis of Disease; 1500; 2; 2-2000; 241-248
0925-4439
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/S0925-4439(99)00105-2
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0925443999001052
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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