NF-κB Activation Is Involved in Regulation of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) by Interleukin-1β

Autores
Cafferata, Eduardo Gustavo Alfredo; González Guerrico, Anatilde M.; Pivetta, Omar Hilario; Santa Coloma, Tomás Antonio
Año de publicación
2001
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Interleukin-1 beta (IL-1b) regulates the levels of cystic fibrosis transmembrane conductance regulator (CFTR) mRNA and protein in the T84 human carcinoma cell line. Here, we studied the role of the transcription factor NF-kB in this regulation. Initially, T84 cells were pretreated with the NF-kB inhibitor pyrrolidine dithiocarbamate. Cells were then stimulated with IL-1b, and CFTR mRNA levels were determined after 4 h by Northern blot analysis. As a result of PDTC treatment, IL-1b stimulation of CFTR mRNA was blocked. On the other hand, daunorubicin, an NF-kB activator, increased the steady-state levels of CFTR mRNA. Furthermore, after treatment with IL-1b for 1 h, cytoplasmic IkBa degradation occurred simultaneously with translocation of p65 into the nucleus. The T84 cells were also transduced with an adenoviral vector expressing a dominant negative form of IkBa, which prevents IkBa phosphorylation and the subsequent nuclear translocation of NF-kB. After viral transduction, the cells were stimulated with IL-1b for 4 h, and CFTR mRNA levels were measured by Northern blot analysis. The stimulation of CFTR, induced by IL-1b, was also blocked in the presence of the dominant negative mutant. These results indicate that NF-kB is involved in the pathway by which IL-1b regulates CFTR.
Fil: Cafferata, Eduardo Gustavo Alfredo. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán". Centro Nacional de Genética Médica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: González Guerrico, Anatilde M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Pivetta, Omar Hilario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán". Centro Nacional de Genética Médica; Argentina
Fil: Santa Coloma, Tomás Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Materia
Cystic fibrosis
Interleukin 1 beta
NF-κB inhibitor
Cystic fibrosis transmembrane conductance regulator
Pyrrolidine dithiocarbamate
Electrophoretic mobility shift assay
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/153236
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/153236
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling NF-κB Activation Is Involved in Regulation of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) by Interleukin-1βCafferata, Eduardo Gustavo AlfredoGonzález Guerrico, Anatilde M.Pivetta, Omar HilarioSanta Coloma, Tomás AntonioCystic fibrosisInterleukin 1 betaNF-κB inhibitorCystic fibrosis transmembrane conductance regulatorPyrrolidine dithiocarbamateElectrophoretic mobility shift assayhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Interleukin-1 beta (IL-1b) regulates the levels of cystic fibrosis transmembrane conductance regulator (CFTR) mRNA and protein in the T84 human carcinoma cell line. Here, we studied the role of the transcription factor NF-kB in this regulation. Initially, T84 cells were pretreated with the NF-kB inhibitor pyrrolidine dithiocarbamate. Cells were then stimulated with IL-1b, and CFTR mRNA levels were determined after 4 h by Northern blot analysis. As a result of PDTC treatment, IL-1b stimulation of CFTR mRNA was blocked. On the other hand, daunorubicin, an NF-kB activator, increased the steady-state levels of CFTR mRNA. Furthermore, after treatment with IL-1b for 1 h, cytoplasmic IkBa degradation occurred simultaneously with translocation of p65 into the nucleus. The T84 cells were also transduced with an adenoviral vector expressing a dominant negative form of IkBa, which prevents IkBa phosphorylation and the subsequent nuclear translocation of NF-kB. After viral transduction, the cells were stimulated with IL-1b for 4 h, and CFTR mRNA levels were measured by Northern blot analysis. The stimulation of CFTR, induced by IL-1b, was also blocked in the presence of the dominant negative mutant. These results indicate that NF-kB is involved in the pathway by which IL-1b regulates CFTR.Fil: Cafferata, Eduardo Gustavo Alfredo. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán". Centro Nacional de Genética Médica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: González Guerrico, Anatilde M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Pivetta, Omar Hilario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán". Centro Nacional de Genética Médica; ArgentinaFil: Santa Coloma, Tomás Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaAmerican Society for Biochemistry and Molecular Biology2001-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/153236Cafferata, Eduardo Gustavo Alfredo; González Guerrico, Anatilde M.; Pivetta, Omar Hilario; Santa Coloma, Tomás Antonio; NF-κB Activation Is Involved in Regulation of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) by Interleukin-1β; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry; 276; 18; 5-2001; 15441-154440021-92581083-351XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0021925819568466info:eu-repo/semantics/altIdentifier/doi/10.1074/jbc.M010061200info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:12Zoai:ri.conicet.gov.ar:11336/153236instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:12.754CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv NF-κB Activation Is Involved in Regulation of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) by Interleukin-1β
title NF-κB Activation Is Involved in Regulation of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) by Interleukin-1β
spellingShingle NF-κB Activation Is Involved in Regulation of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) by Interleukin-1β
Cafferata, Eduardo Gustavo Alfredo
Cystic fibrosis
Interleukin 1 beta
NF-κB inhibitor
Cystic fibrosis transmembrane conductance regulator
Pyrrolidine dithiocarbamate
Electrophoretic mobility shift assay
title_short NF-κB Activation Is Involved in Regulation of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) by Interleukin-1β
title_full NF-κB Activation Is Involved in Regulation of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) by Interleukin-1β
title_fullStr NF-κB Activation Is Involved in Regulation of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) by Interleukin-1β
title_full_unstemmed NF-κB Activation Is Involved in Regulation of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) by Interleukin-1β
title_sort NF-κB Activation Is Involved in Regulation of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) by Interleukin-1β
dc.creator.none.fl_str_mv Cafferata, Eduardo Gustavo Alfredo
González Guerrico, Anatilde M.
Pivetta, Omar Hilario
Santa Coloma, Tomás Antonio
author Cafferata, Eduardo Gustavo Alfredo
author_facet Cafferata, Eduardo Gustavo Alfredo
González Guerrico, Anatilde M.
Pivetta, Omar Hilario
Santa Coloma, Tomás Antonio
author_role author
author2 González Guerrico, Anatilde M.
Pivetta, Omar Hilario
Santa Coloma, Tomás Antonio
author2_role author
author
author
dc.subject.none.fl_str_mv Cystic fibrosis
Interleukin 1 beta
NF-κB inhibitor
Cystic fibrosis transmembrane conductance regulator
Pyrrolidine dithiocarbamate
Electrophoretic mobility shift assay
topic Cystic fibrosis
Interleukin 1 beta
NF-κB inhibitor
Cystic fibrosis transmembrane conductance regulator
Pyrrolidine dithiocarbamate
Electrophoretic mobility shift assay
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Interleukin-1 beta (IL-1b) regulates the levels of cystic fibrosis transmembrane conductance regulator (CFTR) mRNA and protein in the T84 human carcinoma cell line. Here, we studied the role of the transcription factor NF-kB in this regulation. Initially, T84 cells were pretreated with the NF-kB inhibitor pyrrolidine dithiocarbamate. Cells were then stimulated with IL-1b, and CFTR mRNA levels were determined after 4 h by Northern blot analysis. As a result of PDTC treatment, IL-1b stimulation of CFTR mRNA was blocked. On the other hand, daunorubicin, an NF-kB activator, increased the steady-state levels of CFTR mRNA. Furthermore, after treatment with IL-1b for 1 h, cytoplasmic IkBa degradation occurred simultaneously with translocation of p65 into the nucleus. The T84 cells were also transduced with an adenoviral vector expressing a dominant negative form of IkBa, which prevents IkBa phosphorylation and the subsequent nuclear translocation of NF-kB. After viral transduction, the cells were stimulated with IL-1b for 4 h, and CFTR mRNA levels were measured by Northern blot analysis. The stimulation of CFTR, induced by IL-1b, was also blocked in the presence of the dominant negative mutant. These results indicate that NF-kB is involved in the pathway by which IL-1b regulates CFTR.
Fil: Cafferata, Eduardo Gustavo Alfredo. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán". Centro Nacional de Genética Médica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: González Guerrico, Anatilde M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Pivetta, Omar Hilario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán". Centro Nacional de Genética Médica; Argentina
Fil: Santa Coloma, Tomás Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
description Interleukin-1 beta (IL-1b) regulates the levels of cystic fibrosis transmembrane conductance regulator (CFTR) mRNA and protein in the T84 human carcinoma cell line. Here, we studied the role of the transcription factor NF-kB in this regulation. Initially, T84 cells were pretreated with the NF-kB inhibitor pyrrolidine dithiocarbamate. Cells were then stimulated with IL-1b, and CFTR mRNA levels were determined after 4 h by Northern blot analysis. As a result of PDTC treatment, IL-1b stimulation of CFTR mRNA was blocked. On the other hand, daunorubicin, an NF-kB activator, increased the steady-state levels of CFTR mRNA. Furthermore, after treatment with IL-1b for 1 h, cytoplasmic IkBa degradation occurred simultaneously with translocation of p65 into the nucleus. The T84 cells were also transduced with an adenoviral vector expressing a dominant negative form of IkBa, which prevents IkBa phosphorylation and the subsequent nuclear translocation of NF-kB. After viral transduction, the cells were stimulated with IL-1b for 4 h, and CFTR mRNA levels were measured by Northern blot analysis. The stimulation of CFTR, induced by IL-1b, was also blocked in the presence of the dominant negative mutant. These results indicate that NF-kB is involved in the pathway by which IL-1b regulates CFTR.
publishDate 2001
dc.date.none.fl_str_mv 2001-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/153236
Cafferata, Eduardo Gustavo Alfredo; González Guerrico, Anatilde M.; Pivetta, Omar Hilario; Santa Coloma, Tomás Antonio; NF-κB Activation Is Involved in Regulation of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) by Interleukin-1β; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry; 276; 18; 5-2001; 15441-15444
0021-9258
1083-351X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/153236
identifier_str_mv Cafferata, Eduardo Gustavo Alfredo; González Guerrico, Anatilde M.; Pivetta, Omar Hilario; Santa Coloma, Tomás Antonio; NF-κB Activation Is Involved in Regulation of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) by Interleukin-1β; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry; 276; 18; 5-2001; 15441-15444
0021-9258
1083-351X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0021925819568466
info:eu-repo/semantics/altIdentifier/doi/10.1074/jbc.M010061200
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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