Modulation of extracellular ph in cystic fibrosis cells by egfr

Autores
Massip Copiz, María Macarena; Valdivieso, Ángel Gabriel; Asensio, Cristian Jorge Alejandro; Santa Coloma, Tomás Antonio
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Cystic fibrosis (CF) is an inherited disease caused by mutations inthe CFTR (CF transmembrane conductance regulator) gene. It isprincipally characterized by chronic infections and persistent inflammation in lungs. One of the hypotheses that tried to explain the increased susceptibility to lung infections is the reduction in the airwaysurface liquid (ASL) pH. The changes observed in the extracellularpH (pHe) could be a consequence of a reduced bicarbonate transport through CFTR and also an increase in lactic acid secretion.Considering that the EGFR pathway is affected in the CF phenotype, the aim of the present work was to determine if the EGFRpathway is involved in the extracellular pH regulation in CF cells.Two cellular models were used: IB3-1 cells (CF cells) and C38 cells(IB3-1 ?corrected? cells). Together with the extracellular pH and lacticacid (lactate) secretion measurements, LDH expression and activitywere performed by RT-PCR, Western Blot, flow cytometry, confocalmicroscopy and spectrophotometric techniques. The results reported a decrease in pH in the extracellular medium culture (p<0.05) inIB3-1 cells concomitantly with an increased in lactate secretion andboth LDH expression and activity (p<0.05) compared with C38 cells.Studying the role of EGFR in pH regulation, we observed that inhibition of EGFR restores the pH in the extracellular medium (p<0.05),the lactate secretion (p<0.05) and the LDH expression and activity (p<0.05) in CF cells. In conclusion, our results confirmed that aconstitutive EGFR activation is partially involved in the regulationof pHe, lactic acid secretion and LDH expression and activity bythe CFTR channel. The low pH microenvironment observed in CFcells could interfere with immunological functions and promote theestablishment of infections in patients with CF.
Fil: Massip Copiz, María Macarena. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
Fil: Valdivieso, Ángel Gabriel. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
Fil: Asensio, Cristian Jorge Alejandro. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
Fil: Santa Coloma, Tomás Antonio. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión anual de la Sociedad Argentina de Immunología; LIII Reunión anual de la Asociación Argentina de Farmacología Experimental y XI Reunión anual de la Asociación Argentina de Nanomedicinas
Argentina
Sociedad Argentina de Inmunología
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Nanomedicinas
Materia
PH
CYSTIC FIBROSIS
CFTR
EGFR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/280749
Acceder
id CONICETDig_abddc11bf57a4eaee21b694ff1f55679
oai_identifier_str oai:ri.conicet.gov.ar:11336/280749
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Modulation of extracellular ph in cystic fibrosis cells by egfrMassip Copiz, María MacarenaValdivieso, Ángel GabrielAsensio, Cristian Jorge AlejandroSanta Coloma, Tomás AntonioPHCYSTIC FIBROSISCFTREGFRhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Cystic fibrosis (CF) is an inherited disease caused by mutations inthe CFTR (CF transmembrane conductance regulator) gene. It isprincipally characterized by chronic infections and persistent inflammation in lungs. One of the hypotheses that tried to explain the increased susceptibility to lung infections is the reduction in the airwaysurface liquid (ASL) pH. The changes observed in the extracellularpH (pHe) could be a consequence of a reduced bicarbonate transport through CFTR and also an increase in lactic acid secretion.Considering that the EGFR pathway is affected in the CF phenotype, the aim of the present work was to determine if the EGFRpathway is involved in the extracellular pH regulation in CF cells.Two cellular models were used: IB3-1 cells (CF cells) and C38 cells(IB3-1 ?corrected? cells). Together with the extracellular pH and lacticacid (lactate) secretion measurements, LDH expression and activitywere performed by RT-PCR, Western Blot, flow cytometry, confocalmicroscopy and spectrophotometric techniques. The results reported a decrease in pH in the extracellular medium culture (p<0.05) inIB3-1 cells concomitantly with an increased in lactate secretion andboth LDH expression and activity (p<0.05) compared with C38 cells.Studying the role of EGFR in pH regulation, we observed that inhibition of EGFR restores the pH in the extracellular medium (p<0.05),the lactate secretion (p<0.05) and the LDH expression and activity (p<0.05) in CF cells. In conclusion, our results confirmed that aconstitutive EGFR activation is partially involved in the regulationof pHe, lactic acid secretion and LDH expression and activity bythe CFTR channel. The low pH microenvironment observed in CFcells could interfere with immunological functions and promote theestablishment of infections in patients with CF.Fil: Massip Copiz, María Macarena. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; ArgentinaFil: Valdivieso, Ángel Gabriel. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; ArgentinaFil: Asensio, Cristian Jorge Alejandro. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; ArgentinaFil: Santa Coloma, Tomás Antonio. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; ArgentinaLXVI Reunión anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión anual de la Sociedad Argentina de Immunología; LIII Reunión anual de la Asociación Argentina de Farmacología Experimental y XI Reunión anual de la Asociación Argentina de NanomedicinasArgentinaSociedad Argentina de InmunologíaAsociación Argentina de Farmacología ExperimentalSociedad Argentina de Investigación ClínicaAsociación Argentina de NanomedicinasFundación Revista Medicina2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/280749Modulation of extracellular ph in cystic fibrosis cells by egfr; LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión anual de la Sociedad Argentina de Immunología; LIII Reunión anual de la Asociación Argentina de Farmacología Experimental y XI Reunión anual de la Asociación Argentina de Nanomedicinas; Argentina; 2021; 250-2501669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.reunionbiociencias.com.ar/wp-content/uploads/2021/11/Revista-Medicina-2021.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-26T10:28:42Zoai:ri.conicet.gov.ar:11336/280749instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-26 10:28:43.259CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Modulation of extracellular ph in cystic fibrosis cells by egfr
title Modulation of extracellular ph in cystic fibrosis cells by egfr
spellingShingle Modulation of extracellular ph in cystic fibrosis cells by egfr
Massip Copiz, María Macarena
PH
CYSTIC FIBROSIS
CFTR
EGFR
title_short Modulation of extracellular ph in cystic fibrosis cells by egfr
title_full Modulation of extracellular ph in cystic fibrosis cells by egfr
title_fullStr Modulation of extracellular ph in cystic fibrosis cells by egfr
title_full_unstemmed Modulation of extracellular ph in cystic fibrosis cells by egfr
title_sort Modulation of extracellular ph in cystic fibrosis cells by egfr
dc.creator.none.fl_str_mv Massip Copiz, María Macarena
Valdivieso, Ángel Gabriel
Asensio, Cristian Jorge Alejandro
Santa Coloma, Tomás Antonio
author Massip Copiz, María Macarena
author_facet Massip Copiz, María Macarena
Valdivieso, Ángel Gabriel
Asensio, Cristian Jorge Alejandro
Santa Coloma, Tomás Antonio
author_role author
author2 Valdivieso, Ángel Gabriel
Asensio, Cristian Jorge Alejandro
Santa Coloma, Tomás Antonio
author2_role author
author
author
dc.subject.none.fl_str_mv PH
CYSTIC FIBROSIS
CFTR
EGFR
topic PH
CYSTIC FIBROSIS
CFTR
EGFR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Cystic fibrosis (CF) is an inherited disease caused by mutations inthe CFTR (CF transmembrane conductance regulator) gene. It isprincipally characterized by chronic infections and persistent inflammation in lungs. One of the hypotheses that tried to explain the increased susceptibility to lung infections is the reduction in the airwaysurface liquid (ASL) pH. The changes observed in the extracellularpH (pHe) could be a consequence of a reduced bicarbonate transport through CFTR and also an increase in lactic acid secretion.Considering that the EGFR pathway is affected in the CF phenotype, the aim of the present work was to determine if the EGFRpathway is involved in the extracellular pH regulation in CF cells.Two cellular models were used: IB3-1 cells (CF cells) and C38 cells(IB3-1 ?corrected? cells). Together with the extracellular pH and lacticacid (lactate) secretion measurements, LDH expression and activitywere performed by RT-PCR, Western Blot, flow cytometry, confocalmicroscopy and spectrophotometric techniques. The results reported a decrease in pH in the extracellular medium culture (p<0.05) inIB3-1 cells concomitantly with an increased in lactate secretion andboth LDH expression and activity (p<0.05) compared with C38 cells.Studying the role of EGFR in pH regulation, we observed that inhibition of EGFR restores the pH in the extracellular medium (p<0.05),the lactate secretion (p<0.05) and the LDH expression and activity (p<0.05) in CF cells. In conclusion, our results confirmed that aconstitutive EGFR activation is partially involved in the regulationof pHe, lactic acid secretion and LDH expression and activity bythe CFTR channel. The low pH microenvironment observed in CFcells could interfere with immunological functions and promote theestablishment of infections in patients with CF.
Fil: Massip Copiz, María Macarena. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
Fil: Valdivieso, Ángel Gabriel. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
Fil: Asensio, Cristian Jorge Alejandro. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
Fil: Santa Coloma, Tomás Antonio. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión anual de la Sociedad Argentina de Immunología; LIII Reunión anual de la Asociación Argentina de Farmacología Experimental y XI Reunión anual de la Asociación Argentina de Nanomedicinas
Argentina
Sociedad Argentina de Inmunología
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Nanomedicinas
description Cystic fibrosis (CF) is an inherited disease caused by mutations inthe CFTR (CF transmembrane conductance regulator) gene. It isprincipally characterized by chronic infections and persistent inflammation in lungs. One of the hypotheses that tried to explain the increased susceptibility to lung infections is the reduction in the airwaysurface liquid (ASL) pH. The changes observed in the extracellularpH (pHe) could be a consequence of a reduced bicarbonate transport through CFTR and also an increase in lactic acid secretion.Considering that the EGFR pathway is affected in the CF phenotype, the aim of the present work was to determine if the EGFRpathway is involved in the extracellular pH regulation in CF cells.Two cellular models were used: IB3-1 cells (CF cells) and C38 cells(IB3-1 ?corrected? cells). Together with the extracellular pH and lacticacid (lactate) secretion measurements, LDH expression and activitywere performed by RT-PCR, Western Blot, flow cytometry, confocalmicroscopy and spectrophotometric techniques. The results reported a decrease in pH in the extracellular medium culture (p<0.05) inIB3-1 cells concomitantly with an increased in lactate secretion andboth LDH expression and activity (p<0.05) compared with C38 cells.Studying the role of EGFR in pH regulation, we observed that inhibition of EGFR restores the pH in the extracellular medium (p<0.05),the lactate secretion (p<0.05) and the LDH expression and activity (p<0.05) in CF cells. In conclusion, our results confirmed that aconstitutive EGFR activation is partially involved in the regulationof pHe, lactic acid secretion and LDH expression and activity bythe CFTR channel. The low pH microenvironment observed in CFcells could interfere with immunological functions and promote theestablishment of infections in patients with CF.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/280749
Modulation of extracellular ph in cystic fibrosis cells by egfr; LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión anual de la Sociedad Argentina de Immunología; LIII Reunión anual de la Asociación Argentina de Farmacología Experimental y XI Reunión anual de la Asociación Argentina de Nanomedicinas; Argentina; 2021; 250-250
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/280749
identifier_str_mv Modulation of extracellular ph in cystic fibrosis cells by egfr; LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión anual de la Sociedad Argentina de Immunología; LIII Reunión anual de la Asociación Argentina de Farmacología Experimental y XI Reunión anual de la Asociación Argentina de Nanomedicinas; Argentina; 2021; 250-250
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.reunionbiociencias.com.ar/wp-content/uploads/2021/11/Revista-Medicina-2021.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.176822

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