A Comparative Genomic Analysis of Epstein–Barr Virus Strains with a Focus on EBV2 Variability

Autores
Blazquez, Ana Catalina; Fellner, María Dolores; Lorenzetti, Mario Alejandro; Preciado, María Victoria
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Most genomic studies on Epstein-Barr virus variability have focused on geographic and pathological association of EBV1 genomes. In contrast, the variability of EBV2 genomes has been less explored, mainly due to its restricted geographic circulation and the lesser number of sequenced EBV2 isolates. In this study, we sequenced and analyzed 28 EBV1, 10 EBV2 genomes from Argentina, which were combined with 239 downloaded complete genomes from other geographic regions, to produce an initial multi-sample.vcf file comprised of 278 EBV genomes. In this context, we identified 1,093/4,541 positions in the viral genome that contribute to variability between viral types, mainly located in the EBNA2, EBNA3 family of genes and the adjacent BZLF1, BZLF2 and BLLF1 genes. We further described that this variability exhibits distinct patterns across Africa, South America, and Southeast Asia. Compared to EBV1, EBV2 genomes showed fewer variable positions relative to its reference genome (Wilcoxon test, p = 0.0001). Principal component analysis revealed that EBV2 genomes from Southeast Asia segregate independently from those from South America (Wilcoxon test, Bonferroni correction; p = 1.1 x 10-7) and Africa (Wilcoxon test, Bonferroni correction; p = 2.6 x 10-9). Additionally, we identified those precise variable positions with geographic segregation strength: 1,135/3,666 in EBV1 and 380/3,276 in EBV2. Furthermore, the distribution of variable positions along the genome disclosed a close relation for EBV2 isolates from Africa and South America as compared to isolates from Southeast Asia. Although our analysis is limited to EBV2 genomes isolated from three geographic regions, it is to the best of our knowledge, the first study to comprehensively characterize the geographic variability of the complete EBV2 genome. These findings underscore the geographic and genetic diversity of EBV2 genomes and contribute to understanding EBV´s evolutionary dynamics and potential regional adaptations. This research enhances our understanding of EBV2 genomic variability, supporting future epidemiological studies and advancing the knowledge base for targeted treatments and vaccine development for EBV-associated diseases.
Fil: Blazquez, Ana Catalina. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; Argentina
Fil: Fellner, María Dolores. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Lorenzetti, Mario Alejandro. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; Argentina
Fil: Preciado, María Victoria. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; Argentina
Materia
Epstein Barr virus
EBV2
Sequence Variation
South America
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/276543
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/276543
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A Comparative Genomic Analysis of Epstein–Barr Virus Strains with a Focus on EBV2 VariabilityBlazquez, Ana CatalinaFellner, María DoloresLorenzetti, Mario AlejandroPreciado, María VictoriaEpstein Barr virusEBV2Sequence VariationSouth Americahttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3Most genomic studies on Epstein-Barr virus variability have focused on geographic and pathological association of EBV1 genomes. In contrast, the variability of EBV2 genomes has been less explored, mainly due to its restricted geographic circulation and the lesser number of sequenced EBV2 isolates. In this study, we sequenced and analyzed 28 EBV1, 10 EBV2 genomes from Argentina, which were combined with 239 downloaded complete genomes from other geographic regions, to produce an initial multi-sample.vcf file comprised of 278 EBV genomes. In this context, we identified 1,093/4,541 positions in the viral genome that contribute to variability between viral types, mainly located in the EBNA2, EBNA3 family of genes and the adjacent BZLF1, BZLF2 and BLLF1 genes. We further described that this variability exhibits distinct patterns across Africa, South America, and Southeast Asia. Compared to EBV1, EBV2 genomes showed fewer variable positions relative to its reference genome (Wilcoxon test, p = 0.0001). Principal component analysis revealed that EBV2 genomes from Southeast Asia segregate independently from those from South America (Wilcoxon test, Bonferroni correction; p = 1.1 x 10-7) and Africa (Wilcoxon test, Bonferroni correction; p = 2.6 x 10-9). Additionally, we identified those precise variable positions with geographic segregation strength: 1,135/3,666 in EBV1 and 380/3,276 in EBV2. Furthermore, the distribution of variable positions along the genome disclosed a close relation for EBV2 isolates from Africa and South America as compared to isolates from Southeast Asia. Although our analysis is limited to EBV2 genomes isolated from three geographic regions, it is to the best of our knowledge, the first study to comprehensively characterize the geographic variability of the complete EBV2 genome. These findings underscore the geographic and genetic diversity of EBV2 genomes and contribute to understanding EBV´s evolutionary dynamics and potential regional adaptations. This research enhances our understanding of EBV2 genomic variability, supporting future epidemiological studies and advancing the knowledge base for targeted treatments and vaccine development for EBV-associated diseases.Fil: Blazquez, Ana Catalina. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; ArgentinaFil: Fellner, María Dolores. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: Lorenzetti, Mario Alejandro. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; ArgentinaFil: Preciado, María Victoria. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; ArgentinaMolecular Diversity Preservation International2025-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/276543Blazquez, Ana Catalina; Fellner, María Dolores; Lorenzetti, Mario Alejandro; Preciado, María Victoria; A Comparative Genomic Analysis of Epstein–Barr Virus Strains with a Focus on EBV2 Variability; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 26; 6; 3-2025; 1-201422-0067CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/26/6/2708info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms26062708info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-01-08T12:49:10Zoai:ri.conicet.gov.ar:11336/276543instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-01-08 12:49:10.52CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A Comparative Genomic Analysis of Epstein–Barr Virus Strains with a Focus on EBV2 Variability
title A Comparative Genomic Analysis of Epstein–Barr Virus Strains with a Focus on EBV2 Variability
spellingShingle A Comparative Genomic Analysis of Epstein–Barr Virus Strains with a Focus on EBV2 Variability
Blazquez, Ana Catalina
Epstein Barr virus
EBV2
Sequence Variation
South America
title_short A Comparative Genomic Analysis of Epstein–Barr Virus Strains with a Focus on EBV2 Variability
title_full A Comparative Genomic Analysis of Epstein–Barr Virus Strains with a Focus on EBV2 Variability
title_fullStr A Comparative Genomic Analysis of Epstein–Barr Virus Strains with a Focus on EBV2 Variability
title_full_unstemmed A Comparative Genomic Analysis of Epstein–Barr Virus Strains with a Focus on EBV2 Variability
title_sort A Comparative Genomic Analysis of Epstein–Barr Virus Strains with a Focus on EBV2 Variability
dc.creator.none.fl_str_mv Blazquez, Ana Catalina
Fellner, María Dolores
Lorenzetti, Mario Alejandro
Preciado, María Victoria
author Blazquez, Ana Catalina
author_facet Blazquez, Ana Catalina
Fellner, María Dolores
Lorenzetti, Mario Alejandro
Preciado, María Victoria
author_role author
author2 Fellner, María Dolores
Lorenzetti, Mario Alejandro
Preciado, María Victoria
author2_role author
author
author
dc.subject.none.fl_str_mv Epstein Barr virus
EBV2
Sequence Variation
South America
topic Epstein Barr virus
EBV2
Sequence Variation
South America
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Most genomic studies on Epstein-Barr virus variability have focused on geographic and pathological association of EBV1 genomes. In contrast, the variability of EBV2 genomes has been less explored, mainly due to its restricted geographic circulation and the lesser number of sequenced EBV2 isolates. In this study, we sequenced and analyzed 28 EBV1, 10 EBV2 genomes from Argentina, which were combined with 239 downloaded complete genomes from other geographic regions, to produce an initial multi-sample.vcf file comprised of 278 EBV genomes. In this context, we identified 1,093/4,541 positions in the viral genome that contribute to variability between viral types, mainly located in the EBNA2, EBNA3 family of genes and the adjacent BZLF1, BZLF2 and BLLF1 genes. We further described that this variability exhibits distinct patterns across Africa, South America, and Southeast Asia. Compared to EBV1, EBV2 genomes showed fewer variable positions relative to its reference genome (Wilcoxon test, p = 0.0001). Principal component analysis revealed that EBV2 genomes from Southeast Asia segregate independently from those from South America (Wilcoxon test, Bonferroni correction; p = 1.1 x 10-7) and Africa (Wilcoxon test, Bonferroni correction; p = 2.6 x 10-9). Additionally, we identified those precise variable positions with geographic segregation strength: 1,135/3,666 in EBV1 and 380/3,276 in EBV2. Furthermore, the distribution of variable positions along the genome disclosed a close relation for EBV2 isolates from Africa and South America as compared to isolates from Southeast Asia. Although our analysis is limited to EBV2 genomes isolated from three geographic regions, it is to the best of our knowledge, the first study to comprehensively characterize the geographic variability of the complete EBV2 genome. These findings underscore the geographic and genetic diversity of EBV2 genomes and contribute to understanding EBV´s evolutionary dynamics and potential regional adaptations. This research enhances our understanding of EBV2 genomic variability, supporting future epidemiological studies and advancing the knowledge base for targeted treatments and vaccine development for EBV-associated diseases.
Fil: Blazquez, Ana Catalina. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; Argentina
Fil: Fellner, María Dolores. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Lorenzetti, Mario Alejandro. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; Argentina
Fil: Preciado, María Victoria. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; Argentina
description Most genomic studies on Epstein-Barr virus variability have focused on geographic and pathological association of EBV1 genomes. In contrast, the variability of EBV2 genomes has been less explored, mainly due to its restricted geographic circulation and the lesser number of sequenced EBV2 isolates. In this study, we sequenced and analyzed 28 EBV1, 10 EBV2 genomes from Argentina, which were combined with 239 downloaded complete genomes from other geographic regions, to produce an initial multi-sample.vcf file comprised of 278 EBV genomes. In this context, we identified 1,093/4,541 positions in the viral genome that contribute to variability between viral types, mainly located in the EBNA2, EBNA3 family of genes and the adjacent BZLF1, BZLF2 and BLLF1 genes. We further described that this variability exhibits distinct patterns across Africa, South America, and Southeast Asia. Compared to EBV1, EBV2 genomes showed fewer variable positions relative to its reference genome (Wilcoxon test, p = 0.0001). Principal component analysis revealed that EBV2 genomes from Southeast Asia segregate independently from those from South America (Wilcoxon test, Bonferroni correction; p = 1.1 x 10-7) and Africa (Wilcoxon test, Bonferroni correction; p = 2.6 x 10-9). Additionally, we identified those precise variable positions with geographic segregation strength: 1,135/3,666 in EBV1 and 380/3,276 in EBV2. Furthermore, the distribution of variable positions along the genome disclosed a close relation for EBV2 isolates from Africa and South America as compared to isolates from Southeast Asia. Although our analysis is limited to EBV2 genomes isolated from three geographic regions, it is to the best of our knowledge, the first study to comprehensively characterize the geographic variability of the complete EBV2 genome. These findings underscore the geographic and genetic diversity of EBV2 genomes and contribute to understanding EBV´s evolutionary dynamics and potential regional adaptations. This research enhances our understanding of EBV2 genomic variability, supporting future epidemiological studies and advancing the knowledge base for targeted treatments and vaccine development for EBV-associated diseases.
publishDate 2025
dc.date.none.fl_str_mv 2025-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/276543
Blazquez, Ana Catalina; Fellner, María Dolores; Lorenzetti, Mario Alejandro; Preciado, María Victoria; A Comparative Genomic Analysis of Epstein–Barr Virus Strains with a Focus on EBV2 Variability; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 26; 6; 3-2025; 1-20
1422-0067
CONICET Digital
CONICET
url http://hdl.handle.net/11336/276543
identifier_str_mv Blazquez, Ana Catalina; Fellner, María Dolores; Lorenzetti, Mario Alejandro; Preciado, María Victoria; A Comparative Genomic Analysis of Epstein–Barr Virus Strains with a Focus on EBV2 Variability; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 26; 6; 3-2025; 1-20
1422-0067
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/26/6/2708
info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms26062708
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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