Characterization of Epstein Barr virus latency pattern in Argentine breast carcinoma

Autores
Lorenzetti, Mario Alejandro; de Matteo, Elena Noemí; Gass, Hugo Daniel; Martínez Vazquez, Paula; Lara, María Julia; Gonzalez, Pedro; Preciado, María Victoria; Chabay, Paola Andrea
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Introduction: Epstein-Barr virus (EBV)-associated tumors show different expression patterns of latency genes. Since in breast carcinoma this pattern is not yet fully described, our aim was to characterize EBV latency pattern in our EBV positive breast carcinoma series. Methods: The study was conducted on 71 biopsies of breast carcinoma and in 48 non-neoplastic breast controls. EBNA1, LMP2A and LMP1 expression was assessed by immunohistochemistry with monoclonal antibodies, while viral genomic DNA and EBERs RNA transcripts expression was performed by in situ hybridization. EBV presence was confirmed by PCR. Results: EBV genomic DNA and EBNA1 expression were detected in 31% (22/71) of patients specifically restricted to tumor epithelial cells in breast carcinoma while all breast control samples were negative for both, viral DNA and EBNA1 protein. LMP2A was detected in 73% of EBNA1 positive cases, none of which expressed either LMP1 protein or EBERs transcripts. Conclusions: These findings suggest that EBV expression pattern in the studied biopsies could be different from those previously observed in breast carcinoma cell lines and lead us to suggest a new, EBNA1, LMP2A positive and LMP1 and EBERs negative latency profile in breast carcinoma in our population.
Fil: Lorenzetti, Mario Alejandro. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: de Matteo, Elena Noemí. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gass, Hugo Daniel. Hospital Magdalena Villegas de Martinez; Argentina
Fil: Martínez Vazquez, Paula. Hospital Magdalena Villegas de Martinez; Argentina
Fil: Lara, María Julia. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gonzalez, Pedro. Hospital Magdalena Villegas de Martinez; Argentina
Fil: Preciado, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina
Fil: Chabay, Paola Andrea. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Breast Carcinoma
Epstein Barr virus
LMP2A
EBV latency pattern
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/247958
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/247958
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of Epstein Barr virus latency pattern in Argentine breast carcinomaLorenzetti, Mario Alejandrode Matteo, Elena NoemíGass, Hugo DanielMartínez Vazquez, PaulaLara, María JuliaGonzalez, PedroPreciado, María VictoriaChabay, Paola AndreaBreast CarcinomaEpstein Barr virusLMP2AEBV latency patternhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Introduction: Epstein-Barr virus (EBV)-associated tumors show different expression patterns of latency genes. Since in breast carcinoma this pattern is not yet fully described, our aim was to characterize EBV latency pattern in our EBV positive breast carcinoma series. Methods: The study was conducted on 71 biopsies of breast carcinoma and in 48 non-neoplastic breast controls. EBNA1, LMP2A and LMP1 expression was assessed by immunohistochemistry with monoclonal antibodies, while viral genomic DNA and EBERs RNA transcripts expression was performed by in situ hybridization. EBV presence was confirmed by PCR. Results: EBV genomic DNA and EBNA1 expression were detected in 31% (22/71) of patients specifically restricted to tumor epithelial cells in breast carcinoma while all breast control samples were negative for both, viral DNA and EBNA1 protein. LMP2A was detected in 73% of EBNA1 positive cases, none of which expressed either LMP1 protein or EBERs transcripts. Conclusions: These findings suggest that EBV expression pattern in the studied biopsies could be different from those previously observed in breast carcinoma cell lines and lead us to suggest a new, EBNA1, LMP2A positive and LMP1 and EBERs negative latency profile in breast carcinoma in our population.Fil: Lorenzetti, Mario Alejandro. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: de Matteo, Elena Noemí. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gass, Hugo Daniel. Hospital Magdalena Villegas de Martinez; ArgentinaFil: Martínez Vazquez, Paula. Hospital Magdalena Villegas de Martinez; ArgentinaFil: Lara, María Julia. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gonzalez, Pedro. Hospital Magdalena Villegas de Martinez; ArgentinaFil: Preciado, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; ArgentinaFil: Chabay, Paola Andrea. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaPublic Library of Science2010-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/247958Lorenzetti, Mario Alejandro; de Matteo, Elena Noemí; Gass, Hugo Daniel; Martínez Vazquez, Paula; Lara, María Julia; et al.; Characterization of Epstein Barr virus latency pattern in Argentine breast carcinoma; Public Library of Science; Plos One; 5; 10; 10-2010; 1-71932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0013603info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0013603info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:55:33Zoai:ri.conicet.gov.ar:11336/247958instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:55:34.151CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of Epstein Barr virus latency pattern in Argentine breast carcinoma
title Characterization of Epstein Barr virus latency pattern in Argentine breast carcinoma
spellingShingle Characterization of Epstein Barr virus latency pattern in Argentine breast carcinoma
Lorenzetti, Mario Alejandro
Breast Carcinoma
Epstein Barr virus
LMP2A
EBV latency pattern
title_short Characterization of Epstein Barr virus latency pattern in Argentine breast carcinoma
title_full Characterization of Epstein Barr virus latency pattern in Argentine breast carcinoma
title_fullStr Characterization of Epstein Barr virus latency pattern in Argentine breast carcinoma
title_full_unstemmed Characterization of Epstein Barr virus latency pattern in Argentine breast carcinoma
title_sort Characterization of Epstein Barr virus latency pattern in Argentine breast carcinoma
dc.creator.none.fl_str_mv Lorenzetti, Mario Alejandro
de Matteo, Elena Noemí
Gass, Hugo Daniel
Martínez Vazquez, Paula
Lara, María Julia
Gonzalez, Pedro
Preciado, María Victoria
Chabay, Paola Andrea
author Lorenzetti, Mario Alejandro
author_facet Lorenzetti, Mario Alejandro
de Matteo, Elena Noemí
Gass, Hugo Daniel
Martínez Vazquez, Paula
Lara, María Julia
Gonzalez, Pedro
Preciado, María Victoria
Chabay, Paola Andrea
author_role author
author2 de Matteo, Elena Noemí
Gass, Hugo Daniel
Martínez Vazquez, Paula
Lara, María Julia
Gonzalez, Pedro
Preciado, María Victoria
Chabay, Paola Andrea
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Breast Carcinoma
Epstein Barr virus
LMP2A
EBV latency pattern
topic Breast Carcinoma
Epstein Barr virus
LMP2A
EBV latency pattern
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Introduction: Epstein-Barr virus (EBV)-associated tumors show different expression patterns of latency genes. Since in breast carcinoma this pattern is not yet fully described, our aim was to characterize EBV latency pattern in our EBV positive breast carcinoma series. Methods: The study was conducted on 71 biopsies of breast carcinoma and in 48 non-neoplastic breast controls. EBNA1, LMP2A and LMP1 expression was assessed by immunohistochemistry with monoclonal antibodies, while viral genomic DNA and EBERs RNA transcripts expression was performed by in situ hybridization. EBV presence was confirmed by PCR. Results: EBV genomic DNA and EBNA1 expression were detected in 31% (22/71) of patients specifically restricted to tumor epithelial cells in breast carcinoma while all breast control samples were negative for both, viral DNA and EBNA1 protein. LMP2A was detected in 73% of EBNA1 positive cases, none of which expressed either LMP1 protein or EBERs transcripts. Conclusions: These findings suggest that EBV expression pattern in the studied biopsies could be different from those previously observed in breast carcinoma cell lines and lead us to suggest a new, EBNA1, LMP2A positive and LMP1 and EBERs negative latency profile in breast carcinoma in our population.
Fil: Lorenzetti, Mario Alejandro. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: de Matteo, Elena Noemí. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gass, Hugo Daniel. Hospital Magdalena Villegas de Martinez; Argentina
Fil: Martínez Vazquez, Paula. Hospital Magdalena Villegas de Martinez; Argentina
Fil: Lara, María Julia. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gonzalez, Pedro. Hospital Magdalena Villegas de Martinez; Argentina
Fil: Preciado, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina
Fil: Chabay, Paola Andrea. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Introduction: Epstein-Barr virus (EBV)-associated tumors show different expression patterns of latency genes. Since in breast carcinoma this pattern is not yet fully described, our aim was to characterize EBV latency pattern in our EBV positive breast carcinoma series. Methods: The study was conducted on 71 biopsies of breast carcinoma and in 48 non-neoplastic breast controls. EBNA1, LMP2A and LMP1 expression was assessed by immunohistochemistry with monoclonal antibodies, while viral genomic DNA and EBERs RNA transcripts expression was performed by in situ hybridization. EBV presence was confirmed by PCR. Results: EBV genomic DNA and EBNA1 expression were detected in 31% (22/71) of patients specifically restricted to tumor epithelial cells in breast carcinoma while all breast control samples were negative for both, viral DNA and EBNA1 protein. LMP2A was detected in 73% of EBNA1 positive cases, none of which expressed either LMP1 protein or EBERs transcripts. Conclusions: These findings suggest that EBV expression pattern in the studied biopsies could be different from those previously observed in breast carcinoma cell lines and lead us to suggest a new, EBNA1, LMP2A positive and LMP1 and EBERs negative latency profile in breast carcinoma in our population.
publishDate 2010
dc.date.none.fl_str_mv 2010-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/247958
Lorenzetti, Mario Alejandro; de Matteo, Elena Noemí; Gass, Hugo Daniel; Martínez Vazquez, Paula; Lara, María Julia; et al.; Characterization of Epstein Barr virus latency pattern in Argentine breast carcinoma; Public Library of Science; Plos One; 5; 10; 10-2010; 1-7
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/247958
identifier_str_mv Lorenzetti, Mario Alejandro; de Matteo, Elena Noemí; Gass, Hugo Daniel; Martínez Vazquez, Paula; Lara, María Julia; et al.; Characterization of Epstein Barr virus latency pattern in Argentine breast carcinoma; Public Library of Science; Plos One; 5; 10; 10-2010; 1-7
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0013603
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0013603
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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