Cytogenetic and microtubule array effects of the zineb-containing commercial fungicide formulation Azzurro ® on meristematic root cells of Allium cepa L.

Autores: Andrioli, Nancy Beatriz; Soloneski, Sonia Maria Elsa; Larramendy, Marcelo Luis; Mudry, Marta Dolores
Año de publicación: 2012
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: Zineb [ethylene bis(dithiocarbamate) zinc] is a widely employed foliar fungicide for agricultural and industrial applications. Allium cepa L. is a reliable model for the assessment of xenobiotic genotoxicity and cytotoxicity. We evaluated the effects of the zineb-containing commercial formulation Azzurro ® (70% zineb) in cell cycle stages of the meristem root cells of A. cepa. The mitotic index (MI), chromosomal aberrations at anaphase/telophase (CAs), micronuclei (MN), and abnormalities in immunodetected microtubule structures, e.g., preprophasic band (PPB), mitotic spindle (MS), and phragmoplast (Phrag), were used as end-points. Azzurro ® (1 and 10μg/ml) induced a significant increase in the frequency of CAs (P<0.05), and the higher concentration inhibited the MI (P<0.05) compared to control values. The frequency of MN did not differ from control values at any concentration. Treatment with 1μg/ml Azzurro ® induced a significant increase in the frequency of abnormal PPB (P<0.01), MS (P<0.001), and Phrag (P<0.01) and, at 10μg/ml, enhancements in the frequencies of abnormal MS (P<0.05) and Phrag (P<0.05) were seen. A tubulin immunodetection assay showed that exposure to Azzurro ® interferes with normal assembly of microtubule structures during mitosis. © 2011 Elsevier B.V.
Fil: Andrioli, Nancy Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Soloneski, Sonia Maria Elsa. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Larramendy, Marcelo Luis. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mudry, Marta Dolores. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia: ALLIUM CEPA
CYTOTOXICITY
GENOTOXICITY
MICROTUBULES
ZINEB
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/139000

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spelling	Cytogenetic and microtubule array effects of the zineb-containing commercial fungicide formulation Azzurro ® on meristematic root cells of Allium cepa L.Andrioli, Nancy BeatrizSoloneski, Sonia Maria ElsaLarramendy, Marcelo LuisMudry, Marta DoloresALLIUM CEPACYTOTOXICITYGENOTOXICITYMICROTUBULESZINEBhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Zineb [ethylene bis(dithiocarbamate) zinc] is a widely employed foliar fungicide for agricultural and industrial applications. Allium cepa L. is a reliable model for the assessment of xenobiotic genotoxicity and cytotoxicity. We evaluated the effects of the zineb-containing commercial formulation Azzurro ® (70% zineb) in cell cycle stages of the meristem root cells of A. cepa. The mitotic index (MI), chromosomal aberrations at anaphase/telophase (CAs), micronuclei (MN), and abnormalities in immunodetected microtubule structures, e.g., preprophasic band (PPB), mitotic spindle (MS), and phragmoplast (Phrag), were used as end-points. Azzurro ® (1 and 10μg/ml) induced a significant increase in the frequency of CAs (P<0.05), and the higher concentration inhibited the MI (P<0.05) compared to control values. The frequency of MN did not differ from control values at any concentration. Treatment with 1μg/ml Azzurro ® induced a significant increase in the frequency of abnormal PPB (P<0.01), MS (P<0.001), and Phrag (P<0.01) and, at 10μg/ml, enhancements in the frequencies of abnormal MS (P<0.05) and Phrag (P<0.05) were seen. A tubulin immunodetection assay showed that exposure to Azzurro ® interferes with normal assembly of microtubule structures during mitosis. © 2011 Elsevier B.V.Fil: Andrioli, Nancy Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Soloneski, Sonia Maria Elsa. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Larramendy, Marcelo Luis. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mudry, Marta Dolores. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2012-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/139000Andrioli, Nancy Beatriz; Soloneski, Sonia Maria Elsa; Larramendy, Marcelo Luis; Mudry, Marta Dolores; Cytogenetic and microtubule array effects of the zineb-containing commercial fungicide formulation Azzurro ® on meristematic root cells of Allium cepa L.; Elsevier; Mutation Research-genetic Toxicology; 742; 1-2; 2-2012; 48-531383-5718CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S1383571811003500info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mrgentox.2011.11.014info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:31:08Zoai:ri.conicet.gov.ar:11336/139000instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:31:08.663CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Cytogenetic and microtubule array effects of the zineb-containing commercial fungicide formulation Azzurro ® on meristematic root cells of Allium cepa L.
title	Cytogenetic and microtubule array effects of the zineb-containing commercial fungicide formulation Azzurro ® on meristematic root cells of Allium cepa L.
spellingShingle	Cytogenetic and microtubule array effects of the zineb-containing commercial fungicide formulation Azzurro ® on meristematic root cells of Allium cepa L. Andrioli, Nancy Beatriz ALLIUM CEPA CYTOTOXICITY GENOTOXICITY MICROTUBULES ZINEB
title_short	Cytogenetic and microtubule array effects of the zineb-containing commercial fungicide formulation Azzurro ® on meristematic root cells of Allium cepa L.
title_full	Cytogenetic and microtubule array effects of the zineb-containing commercial fungicide formulation Azzurro ® on meristematic root cells of Allium cepa L.
title_fullStr	Cytogenetic and microtubule array effects of the zineb-containing commercial fungicide formulation Azzurro ® on meristematic root cells of Allium cepa L.
title_full_unstemmed	Cytogenetic and microtubule array effects of the zineb-containing commercial fungicide formulation Azzurro ® on meristematic root cells of Allium cepa L.
title_sort	Cytogenetic and microtubule array effects of the zineb-containing commercial fungicide formulation Azzurro ® on meristematic root cells of Allium cepa L.
dc.creator.none.fl_str_mv	Andrioli, Nancy Beatriz Soloneski, Sonia Maria Elsa Larramendy, Marcelo Luis Mudry, Marta Dolores
author	Andrioli, Nancy Beatriz
author_facet	Andrioli, Nancy Beatriz Soloneski, Sonia Maria Elsa Larramendy, Marcelo Luis Mudry, Marta Dolores
author_role	author
author2	Soloneski, Sonia Maria Elsa Larramendy, Marcelo Luis Mudry, Marta Dolores
author2_role	author author author
dc.subject.none.fl_str_mv	ALLIUM CEPA CYTOTOXICITY GENOTOXICITY MICROTUBULES ZINEB
topic	ALLIUM CEPA CYTOTOXICITY GENOTOXICITY MICROTUBULES ZINEB
purl_subject.fl_str_mv	https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv	Zineb [ethylene bis(dithiocarbamate) zinc] is a widely employed foliar fungicide for agricultural and industrial applications. Allium cepa L. is a reliable model for the assessment of xenobiotic genotoxicity and cytotoxicity. We evaluated the effects of the zineb-containing commercial formulation Azzurro ® (70% zineb) in cell cycle stages of the meristem root cells of A. cepa. The mitotic index (MI), chromosomal aberrations at anaphase/telophase (CAs), micronuclei (MN), and abnormalities in immunodetected microtubule structures, e.g., preprophasic band (PPB), mitotic spindle (MS), and phragmoplast (Phrag), were used as end-points. Azzurro ® (1 and 10μg/ml) induced a significant increase in the frequency of CAs (P<0.05), and the higher concentration inhibited the MI (P<0.05) compared to control values. The frequency of MN did not differ from control values at any concentration. Treatment with 1μg/ml Azzurro ® induced a significant increase in the frequency of abnormal PPB (P<0.01), MS (P<0.001), and Phrag (P<0.01) and, at 10μg/ml, enhancements in the frequencies of abnormal MS (P<0.05) and Phrag (P<0.05) were seen. A tubulin immunodetection assay showed that exposure to Azzurro ® interferes with normal assembly of microtubule structures during mitosis. © 2011 Elsevier B.V. Fil: Andrioli, Nancy Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Soloneski, Sonia Maria Elsa. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Larramendy, Marcelo Luis. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Mudry, Marta Dolores. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description	Zineb [ethylene bis(dithiocarbamate) zinc] is a widely employed foliar fungicide for agricultural and industrial applications. Allium cepa L. is a reliable model for the assessment of xenobiotic genotoxicity and cytotoxicity. We evaluated the effects of the zineb-containing commercial formulation Azzurro ® (70% zineb) in cell cycle stages of the meristem root cells of A. cepa. The mitotic index (MI), chromosomal aberrations at anaphase/telophase (CAs), micronuclei (MN), and abnormalities in immunodetected microtubule structures, e.g., preprophasic band (PPB), mitotic spindle (MS), and phragmoplast (Phrag), were used as end-points. Azzurro ® (1 and 10μg/ml) induced a significant increase in the frequency of CAs (P<0.05), and the higher concentration inhibited the MI (P<0.05) compared to control values. The frequency of MN did not differ from control values at any concentration. Treatment with 1μg/ml Azzurro ® induced a significant increase in the frequency of abnormal PPB (P<0.01), MS (P<0.001), and Phrag (P<0.01) and, at 10μg/ml, enhancements in the frequencies of abnormal MS (P<0.05) and Phrag (P<0.05) were seen. A tubulin immunodetection assay showed that exposure to Azzurro ® interferes with normal assembly of microtubule structures during mitosis. © 2011 Elsevier B.V.
publishDate	2012
dc.date.none.fl_str_mv	2012-02
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/139000 Andrioli, Nancy Beatriz; Soloneski, Sonia Maria Elsa; Larramendy, Marcelo Luis; Mudry, Marta Dolores; Cytogenetic and microtubule array effects of the zineb-containing commercial fungicide formulation Azzurro ® on meristematic root cells of Allium cepa L.; Elsevier; Mutation Research-genetic Toxicology; 742; 1-2; 2-2012; 48-53 1383-5718 CONICET Digital CONICET
url	http://hdl.handle.net/11336/139000
identifier_str_mv	Andrioli, Nancy Beatriz; Soloneski, Sonia Maria Elsa; Larramendy, Marcelo Luis; Mudry, Marta Dolores; Cytogenetic and microtubule array effects of the zineb-containing commercial fungicide formulation Azzurro ® on meristematic root cells of Allium cepa L.; Elsevier; Mutation Research-genetic Toxicology; 742; 1-2; 2-2012; 48-53 1383-5718 CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S1383571811003500 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mrgentox.2011.11.014
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv	application/pdf application/pdf
dc.publisher.none.fl_str_mv	Elsevier
publisher.none.fl_str_mv	Elsevier
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
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repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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Cytogenetic and microtubule array effects of the zineb-containing commercial fungicide formulation Azzurro ® on meristematic root cells of Allium cepa L.

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