Coupling of proteins to nanodiamonds used as pseudo-stationary phases for the determination of neurological disease biomarkers

Autores
López, Romina Vanesa; Sombra, Lorena Luján; Stege, Patricia Wanda
Año de publicación
2018
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
In the last two decades, significant interest has been reported on the use of nanometer sized diamond particles (NDs). These nanoparticles have attracted the interest of the nanoscience and nanotechnology communities, because of the potential use in the manufacturing of bright, low voltage (cold) cathodes and light emitters nanodiamond films. Furthermore, chemical modifications of the surface of the nanoparticles are becoming of significant value for the synthesis of bionanoconjugates. Glucose transporter type 1 (Glut1) deficiency syndrome is a rare genetic metabolic disorder characterized by deficiency of a protein that is required for glucose to cross the blood-brain barrier. In children it affects the nervous system and it can be manifested with a variety of neurological symptoms. The most common symptom is seizures, which usually begin within the first few months of life. However, the symptoms and severity of Glut1 deficiency syndrome can vary substantially from one person to another. If diagnosed in the early stage of the disease formation, it can be of help to improve the quality of life of patients.The aim of this study was to apply NDs as an immunosupport for the determination of Glut1 in a red cells lysate using an affinity capillary electrochromatography technique. The Glut1 antibodies were attached to the NDs and the bionanoconjugate was added to the background electrolyte (BGE) as a pseudostationary phase (PSP).A Beckman P/ACE MDQ capillary electrophoresis instrument was used. The dimensions of the capillary were 50 μm i.d. and 15 cm total length, and the pseudostationary phase system consisted of sodium phosphate 40 mmol/L, pH 7.4, and 7% NDs-Glut1 antibodies covalently bonded by the carboxylated groups present in the nanodiamonds and the amine groups present in the antibodies.The proposed method was successfully employed for the detection of normal Glut1 in a red cells lysate. The use of NDs-Glut1antibodies as pseudo stationary phases in nanoelectrokinetic chromatography (nanoEKC) allows for the determination of the protein of interest with minimal treatment of the samples.
Fil: López, Romina Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Sombra, Lorena Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Stege, Patricia Wanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
LACE 2015 21st Latin-American Symposium on Biotechnology, Biomedical, Biopharmaceutical, and Industrial Applications of Capillary Electrophoresis and Microchip Technology
Cartagena
Colombia
Liga Argentina contra la Epilepsia
Materia
nanoparticles
capillary electrophoresis
Glucose transporter type 1
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/235403

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network_name_str CONICET Digital (CONICET)
spelling Coupling of proteins to nanodiamonds used as pseudo-stationary phases for the determination of neurological disease biomarkersLópez, Romina VanesaSombra, Lorena LujánStege, Patricia Wandananoparticlescapillary electrophoresisGlucose transporter type 1https://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In the last two decades, significant interest has been reported on the use of nanometer sized diamond particles (NDs). These nanoparticles have attracted the interest of the nanoscience and nanotechnology communities, because of the potential use in the manufacturing of bright, low voltage (cold) cathodes and light emitters nanodiamond films. Furthermore, chemical modifications of the surface of the nanoparticles are becoming of significant value for the synthesis of bionanoconjugates. Glucose transporter type 1 (Glut1) deficiency syndrome is a rare genetic metabolic disorder characterized by deficiency of a protein that is required for glucose to cross the blood-brain barrier. In children it affects the nervous system and it can be manifested with a variety of neurological symptoms. The most common symptom is seizures, which usually begin within the first few months of life. However, the symptoms and severity of Glut1 deficiency syndrome can vary substantially from one person to another. If diagnosed in the early stage of the disease formation, it can be of help to improve the quality of life of patients.The aim of this study was to apply NDs as an immunosupport for the determination of Glut1 in a red cells lysate using an affinity capillary electrochromatography technique. The Glut1 antibodies were attached to the NDs and the bionanoconjugate was added to the background electrolyte (BGE) as a pseudostationary phase (PSP).A Beckman P/ACE MDQ capillary electrophoresis instrument was used. The dimensions of the capillary were 50 μm i.d. and 15 cm total length, and the pseudostationary phase system consisted of sodium phosphate 40 mmol/L, pH 7.4, and 7% NDs-Glut1 antibodies covalently bonded by the carboxylated groups present in the nanodiamonds and the amine groups present in the antibodies.The proposed method was successfully employed for the detection of normal Glut1 in a red cells lysate. The use of NDs-Glut1antibodies as pseudo stationary phases in nanoelectrokinetic chromatography (nanoEKC) allows for the determination of the protein of interest with minimal treatment of the samples.Fil: López, Romina Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Sombra, Lorena Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Stege, Patricia Wanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaLACE 2015 21st Latin-American Symposium on Biotechnology, Biomedical, Biopharmaceutical, and Industrial Applications of Capillary Electrophoresis and Microchip TechnologyCartagenaColombiaLiga Argentina contra la EpilepsiaGrupo VLS Print Solution2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/235403Coupling of proteins to nanodiamonds used as pseudo-stationary phases for the determination of neurological disease biomarkers; LACE 2015 21st Latin-American Symposium on Biotechnology, Biomedical, Biopharmaceutical, and Industrial Applications of Capillary Electrophoresis and Microchip Technology; Cartagena; Colombia; 2015; 93-93CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://ainfo.cnptia.embrapa.br/digital/bitstream/item/165588/1/Lace-2015.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:56:57Zoai:ri.conicet.gov.ar:11336/235403instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:56:58.011CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Coupling of proteins to nanodiamonds used as pseudo-stationary phases for the determination of neurological disease biomarkers
title Coupling of proteins to nanodiamonds used as pseudo-stationary phases for the determination of neurological disease biomarkers
spellingShingle Coupling of proteins to nanodiamonds used as pseudo-stationary phases for the determination of neurological disease biomarkers
López, Romina Vanesa
nanoparticles
capillary electrophoresis
Glucose transporter type 1
title_short Coupling of proteins to nanodiamonds used as pseudo-stationary phases for the determination of neurological disease biomarkers
title_full Coupling of proteins to nanodiamonds used as pseudo-stationary phases for the determination of neurological disease biomarkers
title_fullStr Coupling of proteins to nanodiamonds used as pseudo-stationary phases for the determination of neurological disease biomarkers
title_full_unstemmed Coupling of proteins to nanodiamonds used as pseudo-stationary phases for the determination of neurological disease biomarkers
title_sort Coupling of proteins to nanodiamonds used as pseudo-stationary phases for the determination of neurological disease biomarkers
dc.creator.none.fl_str_mv López, Romina Vanesa
Sombra, Lorena Luján
Stege, Patricia Wanda
author López, Romina Vanesa
author_facet López, Romina Vanesa
Sombra, Lorena Luján
Stege, Patricia Wanda
author_role author
author2 Sombra, Lorena Luján
Stege, Patricia Wanda
author2_role author
author
dc.subject.none.fl_str_mv nanoparticles
capillary electrophoresis
Glucose transporter type 1
topic nanoparticles
capillary electrophoresis
Glucose transporter type 1
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In the last two decades, significant interest has been reported on the use of nanometer sized diamond particles (NDs). These nanoparticles have attracted the interest of the nanoscience and nanotechnology communities, because of the potential use in the manufacturing of bright, low voltage (cold) cathodes and light emitters nanodiamond films. Furthermore, chemical modifications of the surface of the nanoparticles are becoming of significant value for the synthesis of bionanoconjugates. Glucose transporter type 1 (Glut1) deficiency syndrome is a rare genetic metabolic disorder characterized by deficiency of a protein that is required for glucose to cross the blood-brain barrier. In children it affects the nervous system and it can be manifested with a variety of neurological symptoms. The most common symptom is seizures, which usually begin within the first few months of life. However, the symptoms and severity of Glut1 deficiency syndrome can vary substantially from one person to another. If diagnosed in the early stage of the disease formation, it can be of help to improve the quality of life of patients.The aim of this study was to apply NDs as an immunosupport for the determination of Glut1 in a red cells lysate using an affinity capillary electrochromatography technique. The Glut1 antibodies were attached to the NDs and the bionanoconjugate was added to the background electrolyte (BGE) as a pseudostationary phase (PSP).A Beckman P/ACE MDQ capillary electrophoresis instrument was used. The dimensions of the capillary were 50 μm i.d. and 15 cm total length, and the pseudostationary phase system consisted of sodium phosphate 40 mmol/L, pH 7.4, and 7% NDs-Glut1 antibodies covalently bonded by the carboxylated groups present in the nanodiamonds and the amine groups present in the antibodies.The proposed method was successfully employed for the detection of normal Glut1 in a red cells lysate. The use of NDs-Glut1antibodies as pseudo stationary phases in nanoelectrokinetic chromatography (nanoEKC) allows for the determination of the protein of interest with minimal treatment of the samples.
Fil: López, Romina Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Sombra, Lorena Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Stege, Patricia Wanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
LACE 2015 21st Latin-American Symposium on Biotechnology, Biomedical, Biopharmaceutical, and Industrial Applications of Capillary Electrophoresis and Microchip Technology
Cartagena
Colombia
Liga Argentina contra la Epilepsia
description In the last two decades, significant interest has been reported on the use of nanometer sized diamond particles (NDs). These nanoparticles have attracted the interest of the nanoscience and nanotechnology communities, because of the potential use in the manufacturing of bright, low voltage (cold) cathodes and light emitters nanodiamond films. Furthermore, chemical modifications of the surface of the nanoparticles are becoming of significant value for the synthesis of bionanoconjugates. Glucose transporter type 1 (Glut1) deficiency syndrome is a rare genetic metabolic disorder characterized by deficiency of a protein that is required for glucose to cross the blood-brain barrier. In children it affects the nervous system and it can be manifested with a variety of neurological symptoms. The most common symptom is seizures, which usually begin within the first few months of life. However, the symptoms and severity of Glut1 deficiency syndrome can vary substantially from one person to another. If diagnosed in the early stage of the disease formation, it can be of help to improve the quality of life of patients.The aim of this study was to apply NDs as an immunosupport for the determination of Glut1 in a red cells lysate using an affinity capillary electrochromatography technique. The Glut1 antibodies were attached to the NDs and the bionanoconjugate was added to the background electrolyte (BGE) as a pseudostationary phase (PSP).A Beckman P/ACE MDQ capillary electrophoresis instrument was used. The dimensions of the capillary were 50 μm i.d. and 15 cm total length, and the pseudostationary phase system consisted of sodium phosphate 40 mmol/L, pH 7.4, and 7% NDs-Glut1 antibodies covalently bonded by the carboxylated groups present in the nanodiamonds and the amine groups present in the antibodies.The proposed method was successfully employed for the detection of normal Glut1 in a red cells lysate. The use of NDs-Glut1antibodies as pseudo stationary phases in nanoelectrokinetic chromatography (nanoEKC) allows for the determination of the protein of interest with minimal treatment of the samples.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/235403
Coupling of proteins to nanodiamonds used as pseudo-stationary phases for the determination of neurological disease biomarkers; LACE 2015 21st Latin-American Symposium on Biotechnology, Biomedical, Biopharmaceutical, and Industrial Applications of Capillary Electrophoresis and Microchip Technology; Cartagena; Colombia; 2015; 93-93
CONICET Digital
CONICET
url http://hdl.handle.net/11336/235403
identifier_str_mv Coupling of proteins to nanodiamonds used as pseudo-stationary phases for the determination of neurological disease biomarkers; LACE 2015 21st Latin-American Symposium on Biotechnology, Biomedical, Biopharmaceutical, and Industrial Applications of Capillary Electrophoresis and Microchip Technology; Cartagena; Colombia; 2015; 93-93
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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