Oxidovanadium(IV) complexes with chrysin and silibinin: anticancer activity and mechanisms of action in a human colon adenocarcinoma model

Autores
Leon, Ignacio Esteban; Cadavid Vargas, Juan Fernando; Tiscornia, Ines Silvia; Porro, V.; Castelli, S.; Katkar, P.; Desideri, A.; Bollati Fogolin, M.; Etcheverry, Susana Beatriz
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Vanadium compounds were studied duringrecent years to be considered as a representative of a newclass of nonplatinum metal antitumor agents in combinationto its low toxicity. On the other hand, flavonoids area wide family of polyphenolic compounds synthesizedby plants that display many interesting biological effects.Since coordination of ligands to metals can improve thepharmacological properties, we report herein, for the firsttime, a exhaustive study of the mechanisms of action oftwo oxidovanadium(IV) complexes with the flavonoids:silibinin Na2[VO(silibinin)2]·6H2O (VOsil) and chrysin[VO(chrysin)2EtOH]2 (VOchrys) on human colonadenocarcinoma derived cell line HT-29. The complexesinhibited the cell viability of colon adenocarcinoma cellsin a dose dependent manner with a greater potency thanthat the free ligands and free metal, demonstrating thebenefit of complexation. The decrease of the ratio of theamount of reduced glutathione to the amount of oxidized glutathione were involved in the deleterious effects of bothcomplexes. Besides, VOchrys caused cell cycle arrest inG2/M phase while VOsil activated caspase 3 and triggeringthe cells directly to apoptosis. Moreover, VOsil diminishedthe NF-kB activation via increasing the sensitivity ofcells to apoptosis. On the other hand, VOsil inhibited thetopoisomerase IB activity concluding that this is importanttarget involved in the anticancer vanadium effects.As a whole, the results presented herein demonstrate thatVOsil has a stronger deleterious action than VOchrys onHT-29 cells, whereby suggesting that Vosil is the potentiallybest candidate for future use in alternative anti-tumortreatments.
Fil: Leon, Ignacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra Bioquímica Patologica; Argentina
Fil: Cadavid Vargas, Juan Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra Bioquímica Patologica; Argentina
Fil: Tiscornia, Ines Silvia. Instituto Pasteur de Montevideo; Uruguay
Fil: Porro, V.. Instituto Pasteur de Montevideo; Uruguay
Fil: Castelli, S.. Universita Tor Vergata; Italia
Fil: Katkar, P.. Universita Tor Vergata; Italia
Fil: Desideri, A.. Universita Tor Vergata; Italia
Fil: Bollati Fogolin, M.. Instituto Pasteur de Montevideo; Uruguay
Fil: Etcheverry, Susana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra Bioquímica Patologica; Argentina
Materia
Metal Based Drug ·
Ht-29 Cells
· Flavonoids ·
Vanadium
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/48372
Acceder
id CONICETDig_3fb903c3c1bd0953331e2b7411a79c81
oai_identifier_str oai:ri.conicet.gov.ar:11336/48372
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Oxidovanadium(IV) complexes with chrysin and silibinin: anticancer activity and mechanisms of action in a human colon adenocarcinoma modelLeon, Ignacio EstebanCadavid Vargas, Juan FernandoTiscornia, Ines SilviaPorro, V.Castelli, S.Katkar, P.Desideri, A.Bollati Fogolin, M.Etcheverry, Susana BeatrizMetal Based Drug ·Ht-29 Cells· Flavonoids ·Vanadiumhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Vanadium compounds were studied duringrecent years to be considered as a representative of a newclass of nonplatinum metal antitumor agents in combinationto its low toxicity. On the other hand, flavonoids area wide family of polyphenolic compounds synthesizedby plants that display many interesting biological effects.Since coordination of ligands to metals can improve thepharmacological properties, we report herein, for the firsttime, a exhaustive study of the mechanisms of action oftwo oxidovanadium(IV) complexes with the flavonoids:silibinin Na2[VO(silibinin)2]·6H2O (VOsil) and chrysin[VO(chrysin)2EtOH]2 (VOchrys) on human colonadenocarcinoma derived cell line HT-29. The complexesinhibited the cell viability of colon adenocarcinoma cellsin a dose dependent manner with a greater potency thanthat the free ligands and free metal, demonstrating thebenefit of complexation. The decrease of the ratio of theamount of reduced glutathione to the amount of oxidized glutathione were involved in the deleterious effects of bothcomplexes. Besides, VOchrys caused cell cycle arrest inG2/M phase while VOsil activated caspase 3 and triggeringthe cells directly to apoptosis. Moreover, VOsil diminishedthe NF-kB activation via increasing the sensitivity ofcells to apoptosis. On the other hand, VOsil inhibited thetopoisomerase IB activity concluding that this is importanttarget involved in the anticancer vanadium effects.As a whole, the results presented herein demonstrate thatVOsil has a stronger deleterious action than VOchrys onHT-29 cells, whereby suggesting that Vosil is the potentiallybest candidate for future use in alternative anti-tumortreatments.Fil: Leon, Ignacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra Bioquímica Patologica; ArgentinaFil: Cadavid Vargas, Juan Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra Bioquímica Patologica; ArgentinaFil: Tiscornia, Ines Silvia. Instituto Pasteur de Montevideo; UruguayFil: Porro, V.. Instituto Pasteur de Montevideo; UruguayFil: Castelli, S.. Universita Tor Vergata; ItaliaFil: Katkar, P.. Universita Tor Vergata; ItaliaFil: Desideri, A.. Universita Tor Vergata; ItaliaFil: Bollati Fogolin, M.. Instituto Pasteur de Montevideo; UruguayFil: Etcheverry, Susana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra Bioquímica Patologica; ArgentinaSpringer2015-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/48372Leon, Ignacio Esteban; Cadavid Vargas, Juan Fernando; Tiscornia, Ines Silvia; Porro, V.; Castelli, S.; et al.; Oxidovanadium(IV) complexes with chrysin and silibinin: anticancer activity and mechanisms of action in a human colon adenocarcinoma model; Springer; Journal of Biological Inorganic Chemistry; 20; 7; 9-2015; 1175-11910949-8257CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00775-015-1298-7info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00775-015-1298-7info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:23:48Zoai:ri.conicet.gov.ar:11336/48372instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:23:48.439CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Oxidovanadium(IV) complexes with chrysin and silibinin: anticancer activity and mechanisms of action in a human colon adenocarcinoma model
title Oxidovanadium(IV) complexes with chrysin and silibinin: anticancer activity and mechanisms of action in a human colon adenocarcinoma model
spellingShingle Oxidovanadium(IV) complexes with chrysin and silibinin: anticancer activity and mechanisms of action in a human colon adenocarcinoma model
Leon, Ignacio Esteban
Metal Based Drug ·
Ht-29 Cells
· Flavonoids ·
Vanadium
title_short Oxidovanadium(IV) complexes with chrysin and silibinin: anticancer activity and mechanisms of action in a human colon adenocarcinoma model
title_full Oxidovanadium(IV) complexes with chrysin and silibinin: anticancer activity and mechanisms of action in a human colon adenocarcinoma model
title_fullStr Oxidovanadium(IV) complexes with chrysin and silibinin: anticancer activity and mechanisms of action in a human colon adenocarcinoma model
title_full_unstemmed Oxidovanadium(IV) complexes with chrysin and silibinin: anticancer activity and mechanisms of action in a human colon adenocarcinoma model
title_sort Oxidovanadium(IV) complexes with chrysin and silibinin: anticancer activity and mechanisms of action in a human colon adenocarcinoma model
dc.creator.none.fl_str_mv Leon, Ignacio Esteban
Cadavid Vargas, Juan Fernando
Tiscornia, Ines Silvia
Porro, V.
Castelli, S.
Katkar, P.
Desideri, A.
Bollati Fogolin, M.
Etcheverry, Susana Beatriz
author Leon, Ignacio Esteban
author_facet Leon, Ignacio Esteban
Cadavid Vargas, Juan Fernando
Tiscornia, Ines Silvia
Porro, V.
Castelli, S.
Katkar, P.
Desideri, A.
Bollati Fogolin, M.
Etcheverry, Susana Beatriz
author_role author
author2 Cadavid Vargas, Juan Fernando
Tiscornia, Ines Silvia
Porro, V.
Castelli, S.
Katkar, P.
Desideri, A.
Bollati Fogolin, M.
Etcheverry, Susana Beatriz
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Metal Based Drug ·
Ht-29 Cells
· Flavonoids ·
Vanadium
topic Metal Based Drug ·
Ht-29 Cells
· Flavonoids ·
Vanadium
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Vanadium compounds were studied duringrecent years to be considered as a representative of a newclass of nonplatinum metal antitumor agents in combinationto its low toxicity. On the other hand, flavonoids area wide family of polyphenolic compounds synthesizedby plants that display many interesting biological effects.Since coordination of ligands to metals can improve thepharmacological properties, we report herein, for the firsttime, a exhaustive study of the mechanisms of action oftwo oxidovanadium(IV) complexes with the flavonoids:silibinin Na2[VO(silibinin)2]·6H2O (VOsil) and chrysin[VO(chrysin)2EtOH]2 (VOchrys) on human colonadenocarcinoma derived cell line HT-29. The complexesinhibited the cell viability of colon adenocarcinoma cellsin a dose dependent manner with a greater potency thanthat the free ligands and free metal, demonstrating thebenefit of complexation. The decrease of the ratio of theamount of reduced glutathione to the amount of oxidized glutathione were involved in the deleterious effects of bothcomplexes. Besides, VOchrys caused cell cycle arrest inG2/M phase while VOsil activated caspase 3 and triggeringthe cells directly to apoptosis. Moreover, VOsil diminishedthe NF-kB activation via increasing the sensitivity ofcells to apoptosis. On the other hand, VOsil inhibited thetopoisomerase IB activity concluding that this is importanttarget involved in the anticancer vanadium effects.As a whole, the results presented herein demonstrate thatVOsil has a stronger deleterious action than VOchrys onHT-29 cells, whereby suggesting that Vosil is the potentiallybest candidate for future use in alternative anti-tumortreatments.
Fil: Leon, Ignacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra Bioquímica Patologica; Argentina
Fil: Cadavid Vargas, Juan Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra Bioquímica Patologica; Argentina
Fil: Tiscornia, Ines Silvia. Instituto Pasteur de Montevideo; Uruguay
Fil: Porro, V.. Instituto Pasteur de Montevideo; Uruguay
Fil: Castelli, S.. Universita Tor Vergata; Italia
Fil: Katkar, P.. Universita Tor Vergata; Italia
Fil: Desideri, A.. Universita Tor Vergata; Italia
Fil: Bollati Fogolin, M.. Instituto Pasteur de Montevideo; Uruguay
Fil: Etcheverry, Susana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra Bioquímica Patologica; Argentina
description Vanadium compounds were studied duringrecent years to be considered as a representative of a newclass of nonplatinum metal antitumor agents in combinationto its low toxicity. On the other hand, flavonoids area wide family of polyphenolic compounds synthesizedby plants that display many interesting biological effects.Since coordination of ligands to metals can improve thepharmacological properties, we report herein, for the firsttime, a exhaustive study of the mechanisms of action oftwo oxidovanadium(IV) complexes with the flavonoids:silibinin Na2[VO(silibinin)2]·6H2O (VOsil) and chrysin[VO(chrysin)2EtOH]2 (VOchrys) on human colonadenocarcinoma derived cell line HT-29. The complexesinhibited the cell viability of colon adenocarcinoma cellsin a dose dependent manner with a greater potency thanthat the free ligands and free metal, demonstrating thebenefit of complexation. The decrease of the ratio of theamount of reduced glutathione to the amount of oxidized glutathione were involved in the deleterious effects of bothcomplexes. Besides, VOchrys caused cell cycle arrest inG2/M phase while VOsil activated caspase 3 and triggeringthe cells directly to apoptosis. Moreover, VOsil diminishedthe NF-kB activation via increasing the sensitivity ofcells to apoptosis. On the other hand, VOsil inhibited thetopoisomerase IB activity concluding that this is importanttarget involved in the anticancer vanadium effects.As a whole, the results presented herein demonstrate thatVOsil has a stronger deleterious action than VOchrys onHT-29 cells, whereby suggesting that Vosil is the potentiallybest candidate for future use in alternative anti-tumortreatments.
publishDate 2015
dc.date.none.fl_str_mv 2015-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/48372
Leon, Ignacio Esteban; Cadavid Vargas, Juan Fernando; Tiscornia, Ines Silvia; Porro, V.; Castelli, S.; et al.; Oxidovanadium(IV) complexes with chrysin and silibinin: anticancer activity and mechanisms of action in a human colon adenocarcinoma model; Springer; Journal of Biological Inorganic Chemistry; 20; 7; 9-2015; 1175-1191
0949-8257
CONICET Digital
CONICET
url http://hdl.handle.net/11336/48372
identifier_str_mv Leon, Ignacio Esteban; Cadavid Vargas, Juan Fernando; Tiscornia, Ines Silvia; Porro, V.; Castelli, S.; et al.; Oxidovanadium(IV) complexes with chrysin and silibinin: anticancer activity and mechanisms of action in a human colon adenocarcinoma model; Springer; Journal of Biological Inorganic Chemistry; 20; 7; 9-2015; 1175-1191
0949-8257
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s00775-015-1298-7
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00775-015-1298-7
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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