Antiproliferative and apoptosis-inducing activity of an oxidovanadium(IV) complex with the flavonoid silibinin against osteosarcoma cells
- Autores
- León, Ignacio Esteban; Porro, V.; Di Virgilio, Ana Laura; Naso, Luciana Gissella; Williams, Patricia Ana María; Bollati-Fogolín, Mariela; Etcheverry, Susana Beatriz
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Flavonoids are a large family of polyphenolic compounds synthesized by plants. They display interesting biological effects mainly related to their antioxidant properties. On the other hand, vanadium compounds also exhibit different biological and pharmacological effects in cell culture and in animal models. Since coordination of ligands to metals can improve or change the pharmacological properties, we report herein, for the first time, a detailed study of the mechanisms of action of an oxidovanadium(IV) complex with the flavonoid silibinin, Na₂[VO(silibinin)₂]·6H₂O (VOsil), in a model of the human osteosarcoma derived cell line MG-63. The complex inhibited the viability of osteosarcoma cells in a dose-dependent manner with a greater potency than that of silibinin and oxidovanadium(IV) (p < 0.01), demonstrating the benefit of complexation. Cytotoxicity and genotoxicity studies also showed a concentration effect for VOsil. The increase in the levels of reactive oxygen species and the decrease of the ratio of the amount of reduced glutathione to the amount of oxidized glutathione were involved in the deleterious effects of the complex. Besides, the complex caused cell cycle arrest and activated caspase 3, triggering apoptosis as determined by flow cytometry. As a whole, these results show the main mechanisms of the deleterious effects of VOsil in the osteosarcoma cell line, demonstrating that this complex is a promising compound for cancer treatments.
Facultad de Ciencias Exactas
Centro de Química Inorgánica - Materia
-
Ciencias Exactas
Química
Bioquímica
Anticancer drug
MG-63 human osteosarcoma cells
Mechanisms of action
Flavonoids
Vanadium - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
.jpg)
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/132016
Ver los metadatos del registro completo
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Antiproliferative and apoptosis-inducing activity of an oxidovanadium(IV) complex with the flavonoid silibinin against osteosarcoma cellsLeón, Ignacio EstebanPorro, V.Di Virgilio, Ana LauraNaso, Luciana GissellaWilliams, Patricia Ana MaríaBollati-Fogolín, MarielaEtcheverry, Susana BeatrizCiencias ExactasQuímicaBioquímicaAnticancer drugMG-63 human osteosarcoma cellsMechanisms of actionFlavonoidsVanadiumFlavonoids are a large family of polyphenolic compounds synthesized by plants. They display interesting biological effects mainly related to their antioxidant properties. On the other hand, vanadium compounds also exhibit different biological and pharmacological effects in cell culture and in animal models. Since coordination of ligands to metals can improve or change the pharmacological properties, we report herein, for the first time, a detailed study of the mechanisms of action of an oxidovanadium(IV) complex with the flavonoid silibinin, Na₂[VO(silibinin)₂]·6H₂O (VOsil), in a model of the human osteosarcoma derived cell line MG-63. The complex inhibited the viability of osteosarcoma cells in a dose-dependent manner with a greater potency than that of silibinin and oxidovanadium(IV) (p < 0.01), demonstrating the benefit of complexation. Cytotoxicity and genotoxicity studies also showed a concentration effect for VOsil. The increase in the levels of reactive oxygen species and the decrease of the ratio of the amount of reduced glutathione to the amount of oxidized glutathione were involved in the deleterious effects of the complex. Besides, the complex caused cell cycle arrest and activated caspase 3, triggering apoptosis as determined by flow cytometry. As a whole, these results show the main mechanisms of the deleterious effects of VOsil in the osteosarcoma cell line, demonstrating that this complex is a promising compound for cancer treatments.Facultad de Ciencias ExactasCentro de Química Inorgánica2014-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf59-74http://sedici.unlp.edu.ar/handle/10915/132016enginfo:eu-repo/semantics/altIdentifier/issn/1432-1327info:eu-repo/semantics/altIdentifier/issn/0949-8257info:eu-repo/semantics/altIdentifier/doi/10.1007/s00775-013-1061-xinfo:eu-repo/semantics/altIdentifier/pmid/24233155info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:27Zoai:sedici.unlp.edu.ar:10915/132016Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:28.11SEDICI (UNLP) - Universidad Nacional de La Platafalse |
| dc.title.none.fl_str_mv |
Antiproliferative and apoptosis-inducing activity of an oxidovanadium(IV) complex with the flavonoid silibinin against osteosarcoma cells |
| title |
Antiproliferative and apoptosis-inducing activity of an oxidovanadium(IV) complex with the flavonoid silibinin against osteosarcoma cells |
| spellingShingle |
Antiproliferative and apoptosis-inducing activity of an oxidovanadium(IV) complex with the flavonoid silibinin against osteosarcoma cells León, Ignacio Esteban Ciencias Exactas Química Bioquímica Anticancer drug MG-63 human osteosarcoma cells Mechanisms of action Flavonoids Vanadium |
| title_short |
Antiproliferative and apoptosis-inducing activity of an oxidovanadium(IV) complex with the flavonoid silibinin against osteosarcoma cells |
| title_full |
Antiproliferative and apoptosis-inducing activity of an oxidovanadium(IV) complex with the flavonoid silibinin against osteosarcoma cells |
| title_fullStr |
Antiproliferative and apoptosis-inducing activity of an oxidovanadium(IV) complex with the flavonoid silibinin against osteosarcoma cells |
| title_full_unstemmed |
Antiproliferative and apoptosis-inducing activity of an oxidovanadium(IV) complex with the flavonoid silibinin against osteosarcoma cells |
| title_sort |
Antiproliferative and apoptosis-inducing activity of an oxidovanadium(IV) complex with the flavonoid silibinin against osteosarcoma cells |
| dc.creator.none.fl_str_mv |
León, Ignacio Esteban Porro, V. Di Virgilio, Ana Laura Naso, Luciana Gissella Williams, Patricia Ana María Bollati-Fogolín, Mariela Etcheverry, Susana Beatriz |
| author |
León, Ignacio Esteban |
| author_facet |
León, Ignacio Esteban Porro, V. Di Virgilio, Ana Laura Naso, Luciana Gissella Williams, Patricia Ana María Bollati-Fogolín, Mariela Etcheverry, Susana Beatriz |
| author_role |
author |
| author2 |
Porro, V. Di Virgilio, Ana Laura Naso, Luciana Gissella Williams, Patricia Ana María Bollati-Fogolín, Mariela Etcheverry, Susana Beatriz |
| author2_role |
author author author author author author |
| dc.subject.none.fl_str_mv |
Ciencias Exactas Química Bioquímica Anticancer drug MG-63 human osteosarcoma cells Mechanisms of action Flavonoids Vanadium |
| topic |
Ciencias Exactas Química Bioquímica Anticancer drug MG-63 human osteosarcoma cells Mechanisms of action Flavonoids Vanadium |
| dc.description.none.fl_txt_mv |
Flavonoids are a large family of polyphenolic compounds synthesized by plants. They display interesting biological effects mainly related to their antioxidant properties. On the other hand, vanadium compounds also exhibit different biological and pharmacological effects in cell culture and in animal models. Since coordination of ligands to metals can improve or change the pharmacological properties, we report herein, for the first time, a detailed study of the mechanisms of action of an oxidovanadium(IV) complex with the flavonoid silibinin, Na₂[VO(silibinin)₂]·6H₂O (VOsil), in a model of the human osteosarcoma derived cell line MG-63. The complex inhibited the viability of osteosarcoma cells in a dose-dependent manner with a greater potency than that of silibinin and oxidovanadium(IV) (p < 0.01), demonstrating the benefit of complexation. Cytotoxicity and genotoxicity studies also showed a concentration effect for VOsil. The increase in the levels of reactive oxygen species and the decrease of the ratio of the amount of reduced glutathione to the amount of oxidized glutathione were involved in the deleterious effects of the complex. Besides, the complex caused cell cycle arrest and activated caspase 3, triggering apoptosis as determined by flow cytometry. As a whole, these results show the main mechanisms of the deleterious effects of VOsil in the osteosarcoma cell line, demonstrating that this complex is a promising compound for cancer treatments. Facultad de Ciencias Exactas Centro de Química Inorgánica |
| description |
Flavonoids are a large family of polyphenolic compounds synthesized by plants. They display interesting biological effects mainly related to their antioxidant properties. On the other hand, vanadium compounds also exhibit different biological and pharmacological effects in cell culture and in animal models. Since coordination of ligands to metals can improve or change the pharmacological properties, we report herein, for the first time, a detailed study of the mechanisms of action of an oxidovanadium(IV) complex with the flavonoid silibinin, Na₂[VO(silibinin)₂]·6H₂O (VOsil), in a model of the human osteosarcoma derived cell line MG-63. The complex inhibited the viability of osteosarcoma cells in a dose-dependent manner with a greater potency than that of silibinin and oxidovanadium(IV) (p < 0.01), demonstrating the benefit of complexation. Cytotoxicity and genotoxicity studies also showed a concentration effect for VOsil. The increase in the levels of reactive oxygen species and the decrease of the ratio of the amount of reduced glutathione to the amount of oxidized glutathione were involved in the deleterious effects of the complex. Besides, the complex caused cell cycle arrest and activated caspase 3, triggering apoptosis as determined by flow cytometry. As a whole, these results show the main mechanisms of the deleterious effects of VOsil in the osteosarcoma cell line, demonstrating that this complex is a promising compound for cancer treatments. |
| publishDate |
2014 |
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2014-01 |
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article |
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publishedVersion |
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http://sedici.unlp.edu.ar/handle/10915/132016 |
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eng |
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eng |
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