Modified bacterial cellulose scaffolds for localized doxorubicin release in human colorectal HT-29 cells

Autores
Cacicedo, Maximiliano Luis; Leon, Ignacio Esteban; Gonzalez, Jimena Soledad; Porto, Luismar M.; Alvarez, Vera Alejandra; Castro, Guillermo Raul
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bacterial cellulose (BC) films modified by the in situ method with the addition of alginate (Alg) during the microbial cultivation of Gluconacetobacter hansenii under static conditions increased the loading of doxorubicin by at least three times. Biophysical analysis of BC-Alg films by scanning electron microscopy, thermogravimetry, X-ray diffraction and FTIR showed a highly homogeneous interpenetrated network scaffold without changes in the BC crystalline structure but with an increased amorphous phase. The main molecular interactions determined by FTIR between both biopolymers clearly suggest high compatibility. These results indicate that alginate plays a key role in the biophysical properties of the hybrid BC matrix. BC-Alg scaffold analysis by nitrogen adsorption isotherms revealed by the Brunauer?Emmett?Teller (BET) method an increase in surface area of about 84% and in pore volume of more than 200%. The Barrett?Joyner?Halenda (BJH) model also showed an increase of about 25% in the pore size compared to the BC film.Loading BC-Alg scaffolds with different amounts of doxorubicin decreased the cell viability of HT-29 human colorectal adenocarcinoma cell line compared to the free Dox from around 95?53% after 24 h and from 63% to 37% after 48 h. Dox kinetic release from the BC-Alg nanocomposite displayed hyperbolic curves related to the different amounts of drug payload and was stable for at least 14 days. The results of the BC-Alg nanocomposites show a promissory potential for anticancer therapies of solid tumors.
Fil: Cacicedo, Maximiliano Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Leon, Ignacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica ; Argentina
Fil: Gonzalez, Jimena Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Porto, Luismar M.. Universidade Federal de Santa Catarina; Brasil
Fil: Alvarez, Vera Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Castro, Guillermo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Materia
Bacterial cellulose
Alginate
Drug delivery
Nanocomposite
Doxorubicin
Cancer therapy
Human colorectal HT-29 cells
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/34537
Acceder
id CONICETDig_77dd19aedbc3bcff8318885e496bafb1
oai_identifier_str oai:ri.conicet.gov.ar:11336/34537
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Modified bacterial cellulose scaffolds for localized doxorubicin release in human colorectal HT-29 cellsCacicedo, Maximiliano LuisLeon, Ignacio EstebanGonzalez, Jimena SoledadPorto, Luismar M.Alvarez, Vera AlejandraCastro, Guillermo RaulBacterial celluloseAlginateDrug deliveryNanocompositeDoxorubicinCancer therapyHuman colorectal HT-29 cellshttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2https://purl.org/becyt/ford/2.5https://purl.org/becyt/ford/2Bacterial cellulose (BC) films modified by the in situ method with the addition of alginate (Alg) during the microbial cultivation of Gluconacetobacter hansenii under static conditions increased the loading of doxorubicin by at least three times. Biophysical analysis of BC-Alg films by scanning electron microscopy, thermogravimetry, X-ray diffraction and FTIR showed a highly homogeneous interpenetrated network scaffold without changes in the BC crystalline structure but with an increased amorphous phase. The main molecular interactions determined by FTIR between both biopolymers clearly suggest high compatibility. These results indicate that alginate plays a key role in the biophysical properties of the hybrid BC matrix. BC-Alg scaffold analysis by nitrogen adsorption isotherms revealed by the Brunauer?Emmett?Teller (BET) method an increase in surface area of about 84% and in pore volume of more than 200%. The Barrett?Joyner?Halenda (BJH) model also showed an increase of about 25% in the pore size compared to the BC film.Loading BC-Alg scaffolds with different amounts of doxorubicin decreased the cell viability of HT-29 human colorectal adenocarcinoma cell line compared to the free Dox from around 95?53% after 24 h and from 63% to 37% after 48 h. Dox kinetic release from the BC-Alg nanocomposite displayed hyperbolic curves related to the different amounts of drug payload and was stable for at least 14 days. The results of the BC-Alg nanocomposites show a promissory potential for anticancer therapies of solid tumors.Fil: Cacicedo, Maximiliano Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Leon, Ignacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica ; ArgentinaFil: Gonzalez, Jimena Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Porto, Luismar M.. Universidade Federal de Santa Catarina; BrasilFil: Alvarez, Vera Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Castro, Guillermo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaElsevier2016-01-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/34537Cacicedo, Maximiliano Luis; Leon, Ignacio Esteban; Gonzalez, Jimena Soledad; Porto, Luismar M.; Alvarez, Vera Alejandra; et al.; Modified bacterial cellulose scaffolds for localized doxorubicin release in human colorectal HT-29 cells; Elsevier; Colloids and Surfaces B: Biointerfaces; 140; 11-1-2016; 421-4290927-7765CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0927776516300066info:eu-repo/semantics/altIdentifier/doi/10.1016/j.colsurfb.2016.01.007info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:33:19Zoai:ri.conicet.gov.ar:11336/34537instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:33:20.007CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Modified bacterial cellulose scaffolds for localized doxorubicin release in human colorectal HT-29 cells
title Modified bacterial cellulose scaffolds for localized doxorubicin release in human colorectal HT-29 cells
spellingShingle Modified bacterial cellulose scaffolds for localized doxorubicin release in human colorectal HT-29 cells
Cacicedo, Maximiliano Luis
Bacterial cellulose
Alginate
Drug delivery
Nanocomposite
Doxorubicin
Cancer therapy
Human colorectal HT-29 cells
title_short Modified bacterial cellulose scaffolds for localized doxorubicin release in human colorectal HT-29 cells
title_full Modified bacterial cellulose scaffolds for localized doxorubicin release in human colorectal HT-29 cells
title_fullStr Modified bacterial cellulose scaffolds for localized doxorubicin release in human colorectal HT-29 cells
title_full_unstemmed Modified bacterial cellulose scaffolds for localized doxorubicin release in human colorectal HT-29 cells
title_sort Modified bacterial cellulose scaffolds for localized doxorubicin release in human colorectal HT-29 cells
dc.creator.none.fl_str_mv Cacicedo, Maximiliano Luis
Leon, Ignacio Esteban
Gonzalez, Jimena Soledad
Porto, Luismar M.
Alvarez, Vera Alejandra
Castro, Guillermo Raul
author Cacicedo, Maximiliano Luis
author_facet Cacicedo, Maximiliano Luis
Leon, Ignacio Esteban
Gonzalez, Jimena Soledad
Porto, Luismar M.
Alvarez, Vera Alejandra
Castro, Guillermo Raul
author_role author
author2 Leon, Ignacio Esteban
Gonzalez, Jimena Soledad
Porto, Luismar M.
Alvarez, Vera Alejandra
Castro, Guillermo Raul
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Bacterial cellulose
Alginate
Drug delivery
Nanocomposite
Doxorubicin
Cancer therapy
Human colorectal HT-29 cells
topic Bacterial cellulose
Alginate
Drug delivery
Nanocomposite
Doxorubicin
Cancer therapy
Human colorectal HT-29 cells
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
https://purl.org/becyt/ford/2.5
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Bacterial cellulose (BC) films modified by the in situ method with the addition of alginate (Alg) during the microbial cultivation of Gluconacetobacter hansenii under static conditions increased the loading of doxorubicin by at least three times. Biophysical analysis of BC-Alg films by scanning electron microscopy, thermogravimetry, X-ray diffraction and FTIR showed a highly homogeneous interpenetrated network scaffold without changes in the BC crystalline structure but with an increased amorphous phase. The main molecular interactions determined by FTIR between both biopolymers clearly suggest high compatibility. These results indicate that alginate plays a key role in the biophysical properties of the hybrid BC matrix. BC-Alg scaffold analysis by nitrogen adsorption isotherms revealed by the Brunauer?Emmett?Teller (BET) method an increase in surface area of about 84% and in pore volume of more than 200%. The Barrett?Joyner?Halenda (BJH) model also showed an increase of about 25% in the pore size compared to the BC film.Loading BC-Alg scaffolds with different amounts of doxorubicin decreased the cell viability of HT-29 human colorectal adenocarcinoma cell line compared to the free Dox from around 95?53% after 24 h and from 63% to 37% after 48 h. Dox kinetic release from the BC-Alg nanocomposite displayed hyperbolic curves related to the different amounts of drug payload and was stable for at least 14 days. The results of the BC-Alg nanocomposites show a promissory potential for anticancer therapies of solid tumors.
Fil: Cacicedo, Maximiliano Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Leon, Ignacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica ; Argentina
Fil: Gonzalez, Jimena Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Porto, Luismar M.. Universidade Federal de Santa Catarina; Brasil
Fil: Alvarez, Vera Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Castro, Guillermo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
description Bacterial cellulose (BC) films modified by the in situ method with the addition of alginate (Alg) during the microbial cultivation of Gluconacetobacter hansenii under static conditions increased the loading of doxorubicin by at least three times. Biophysical analysis of BC-Alg films by scanning electron microscopy, thermogravimetry, X-ray diffraction and FTIR showed a highly homogeneous interpenetrated network scaffold without changes in the BC crystalline structure but with an increased amorphous phase. The main molecular interactions determined by FTIR between both biopolymers clearly suggest high compatibility. These results indicate that alginate plays a key role in the biophysical properties of the hybrid BC matrix. BC-Alg scaffold analysis by nitrogen adsorption isotherms revealed by the Brunauer?Emmett?Teller (BET) method an increase in surface area of about 84% and in pore volume of more than 200%. The Barrett?Joyner?Halenda (BJH) model also showed an increase of about 25% in the pore size compared to the BC film.Loading BC-Alg scaffolds with different amounts of doxorubicin decreased the cell viability of HT-29 human colorectal adenocarcinoma cell line compared to the free Dox from around 95?53% after 24 h and from 63% to 37% after 48 h. Dox kinetic release from the BC-Alg nanocomposite displayed hyperbolic curves related to the different amounts of drug payload and was stable for at least 14 days. The results of the BC-Alg nanocomposites show a promissory potential for anticancer therapies of solid tumors.
publishDate 2016
dc.date.none.fl_str_mv 2016-01-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/34537
Cacicedo, Maximiliano Luis; Leon, Ignacio Esteban; Gonzalez, Jimena Soledad; Porto, Luismar M.; Alvarez, Vera Alejandra; et al.; Modified bacterial cellulose scaffolds for localized doxorubicin release in human colorectal HT-29 cells; Elsevier; Colloids and Surfaces B: Biointerfaces; 140; 11-1-2016; 421-429
0927-7765
CONICET Digital
CONICET
url http://hdl.handle.net/11336/34537
identifier_str_mv Cacicedo, Maximiliano Luis; Leon, Ignacio Esteban; Gonzalez, Jimena Soledad; Porto, Luismar M.; Alvarez, Vera Alejandra; et al.; Modified bacterial cellulose scaffolds for localized doxorubicin release in human colorectal HT-29 cells; Elsevier; Colloids and Surfaces B: Biointerfaces; 140; 11-1-2016; 421-429
0927-7765
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0927776516300066
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.colsurfb.2016.01.007
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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