Genetic diversity of Streptococcus agalactiae from dairy cattle with mastitis in Argentina

Autores
Gerez, María Gabriela; Hernandez, Luciana Belén; Cadona, Jimena Soledad; Sanso, Andrea Mariel; Bustamante, Ana Victoria
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bovine mastitis is an important health problem in dairy cattle which affects the quality and yield of milk and causes significant economic losses in the dairy industry. Streptococcus agalactiae is a Gram-positive and zoonotic bacterium that causes clinical and subclinical contagious bovine mastitis. The main strategy for the control of this pathogen in dairy herds is the antimicrobial therapy. The aim of this study was to determine the genetic diversity of S. agalactiae using Multiple Locus Variable number tandem repeat -VNTR- Analysis (MLVA), serotypes, virulence factors (VF) and antimicrobial resistance (AMR) profiles and to compare the discrimination power of these different methods in strains isolated from cattle with mastitis in Argentinian dairy farms.Eighty-seven S. agalactiae isolates obtained from dairy cattle with mastitis in Argentina were analyzed. The detected serotypes were III, II and Ia. The most frequent virulence and AMR detected genes were cpsA, hylB, PI-2b, cylE, rib, spb1, and tetO and ermB respectively. A total of 36 VF + AMR profiles were detected with a discriminatory power of the method of Ds = 0.96. The MLVA based on six VNTRs showed 29 profiles with a Ds = 0.90. The analysis of VF + AMR + MLVA data together showed 59 profiles with an increased discriminatory power (Ds = 0.98).This study highlights that the MLVA is recommended to add to other methodologies in order to study epidemiological relationships in this species Although within each dairy farm there was a predominance of certain serotypes/virulence profiles, the characteristics did not show total homogeneity, as expected due to the contagious nature of the pathogen. This suggests the incorporation of animals from other herds at some point, a practice not uncommon among dairy farms in Argentina. By other hand, the detection of a same clone in the same farm in different periods confirms that S. agalactiae strains can persist on dairy farms for a long time.
Fil: Gerez, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Hernandez, Luciana Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Cadona, Jimena Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Sanso, Andrea Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Bustamante, Ana Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Materia
STREPTOCOCCUS AGALACTIAE
MASTITIS
DAIRY CATTLE
GENETIC DIVERSITY
MLVA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/262839
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/262839
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genetic diversity of Streptococcus agalactiae from dairy cattle with mastitis in ArgentinaGerez, María GabrielaHernandez, Luciana BelénCadona, Jimena SoledadSanso, Andrea MarielBustamante, Ana VictoriaSTREPTOCOCCUS AGALACTIAEMASTITISDAIRY CATTLEGENETIC DIVERSITYMLVAhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Bovine mastitis is an important health problem in dairy cattle which affects the quality and yield of milk and causes significant economic losses in the dairy industry. Streptococcus agalactiae is a Gram-positive and zoonotic bacterium that causes clinical and subclinical contagious bovine mastitis. The main strategy for the control of this pathogen in dairy herds is the antimicrobial therapy. The aim of this study was to determine the genetic diversity of S. agalactiae using Multiple Locus Variable number tandem repeat -VNTR- Analysis (MLVA), serotypes, virulence factors (VF) and antimicrobial resistance (AMR) profiles and to compare the discrimination power of these different methods in strains isolated from cattle with mastitis in Argentinian dairy farms.Eighty-seven S. agalactiae isolates obtained from dairy cattle with mastitis in Argentina were analyzed. The detected serotypes were III, II and Ia. The most frequent virulence and AMR detected genes were cpsA, hylB, PI-2b, cylE, rib, spb1, and tetO and ermB respectively. A total of 36 VF + AMR profiles were detected with a discriminatory power of the method of Ds = 0.96. The MLVA based on six VNTRs showed 29 profiles with a Ds = 0.90. The analysis of VF + AMR + MLVA data together showed 59 profiles with an increased discriminatory power (Ds = 0.98).This study highlights that the MLVA is recommended to add to other methodologies in order to study epidemiological relationships in this species Although within each dairy farm there was a predominance of certain serotypes/virulence profiles, the characteristics did not show total homogeneity, as expected due to the contagious nature of the pathogen. This suggests the incorporation of animals from other herds at some point, a practice not uncommon among dairy farms in Argentina. By other hand, the detection of a same clone in the same farm in different periods confirms that S. agalactiae strains can persist on dairy farms for a long time.Fil: Gerez, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Hernandez, Luciana Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Cadona, Jimena Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Sanso, Andrea Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Bustamante, Ana Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaBioMed Central2025-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/262839Gerez, María Gabriela; Hernandez, Luciana Belén; Cadona, Jimena Soledad; Sanso, Andrea Mariel; Bustamante, Ana Victoria; Genetic diversity of Streptococcus agalactiae from dairy cattle with mastitis in Argentina; BioMed Central; BMC Veterinary Research; 21; 1; 5-2025; 1-111746-6148CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-025-04584-4info:eu-repo/semantics/altIdentifier/doi/10.1186/s12917-025-04584-4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:23:01Zoai:ri.conicet.gov.ar:11336/262839instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:23:01.521CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genetic diversity of Streptococcus agalactiae from dairy cattle with mastitis in Argentina
title Genetic diversity of Streptococcus agalactiae from dairy cattle with mastitis in Argentina
spellingShingle Genetic diversity of Streptococcus agalactiae from dairy cattle with mastitis in Argentina
Gerez, María Gabriela
STREPTOCOCCUS AGALACTIAE
MASTITIS
DAIRY CATTLE
GENETIC DIVERSITY
MLVA
title_short Genetic diversity of Streptococcus agalactiae from dairy cattle with mastitis in Argentina
title_full Genetic diversity of Streptococcus agalactiae from dairy cattle with mastitis in Argentina
title_fullStr Genetic diversity of Streptococcus agalactiae from dairy cattle with mastitis in Argentina
title_full_unstemmed Genetic diversity of Streptococcus agalactiae from dairy cattle with mastitis in Argentina
title_sort Genetic diversity of Streptococcus agalactiae from dairy cattle with mastitis in Argentina
dc.creator.none.fl_str_mv Gerez, María Gabriela
Hernandez, Luciana Belén
Cadona, Jimena Soledad
Sanso, Andrea Mariel
Bustamante, Ana Victoria
author Gerez, María Gabriela
author_facet Gerez, María Gabriela
Hernandez, Luciana Belén
Cadona, Jimena Soledad
Sanso, Andrea Mariel
Bustamante, Ana Victoria
author_role author
author2 Hernandez, Luciana Belén
Cadona, Jimena Soledad
Sanso, Andrea Mariel
Bustamante, Ana Victoria
author2_role author
author
author
author
dc.subject.none.fl_str_mv STREPTOCOCCUS AGALACTIAE
MASTITIS
DAIRY CATTLE
GENETIC DIVERSITY
MLVA
topic STREPTOCOCCUS AGALACTIAE
MASTITIS
DAIRY CATTLE
GENETIC DIVERSITY
MLVA
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Bovine mastitis is an important health problem in dairy cattle which affects the quality and yield of milk and causes significant economic losses in the dairy industry. Streptococcus agalactiae is a Gram-positive and zoonotic bacterium that causes clinical and subclinical contagious bovine mastitis. The main strategy for the control of this pathogen in dairy herds is the antimicrobial therapy. The aim of this study was to determine the genetic diversity of S. agalactiae using Multiple Locus Variable number tandem repeat -VNTR- Analysis (MLVA), serotypes, virulence factors (VF) and antimicrobial resistance (AMR) profiles and to compare the discrimination power of these different methods in strains isolated from cattle with mastitis in Argentinian dairy farms.Eighty-seven S. agalactiae isolates obtained from dairy cattle with mastitis in Argentina were analyzed. The detected serotypes were III, II and Ia. The most frequent virulence and AMR detected genes were cpsA, hylB, PI-2b, cylE, rib, spb1, and tetO and ermB respectively. A total of 36 VF + AMR profiles were detected with a discriminatory power of the method of Ds = 0.96. The MLVA based on six VNTRs showed 29 profiles with a Ds = 0.90. The analysis of VF + AMR + MLVA data together showed 59 profiles with an increased discriminatory power (Ds = 0.98).This study highlights that the MLVA is recommended to add to other methodologies in order to study epidemiological relationships in this species Although within each dairy farm there was a predominance of certain serotypes/virulence profiles, the characteristics did not show total homogeneity, as expected due to the contagious nature of the pathogen. This suggests the incorporation of animals from other herds at some point, a practice not uncommon among dairy farms in Argentina. By other hand, the detection of a same clone in the same farm in different periods confirms that S. agalactiae strains can persist on dairy farms for a long time.
Fil: Gerez, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Hernandez, Luciana Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Cadona, Jimena Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Sanso, Andrea Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Bustamante, Ana Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
description Bovine mastitis is an important health problem in dairy cattle which affects the quality and yield of milk and causes significant economic losses in the dairy industry. Streptococcus agalactiae is a Gram-positive and zoonotic bacterium that causes clinical and subclinical contagious bovine mastitis. The main strategy for the control of this pathogen in dairy herds is the antimicrobial therapy. The aim of this study was to determine the genetic diversity of S. agalactiae using Multiple Locus Variable number tandem repeat -VNTR- Analysis (MLVA), serotypes, virulence factors (VF) and antimicrobial resistance (AMR) profiles and to compare the discrimination power of these different methods in strains isolated from cattle with mastitis in Argentinian dairy farms.Eighty-seven S. agalactiae isolates obtained from dairy cattle with mastitis in Argentina were analyzed. The detected serotypes were III, II and Ia. The most frequent virulence and AMR detected genes were cpsA, hylB, PI-2b, cylE, rib, spb1, and tetO and ermB respectively. A total of 36 VF + AMR profiles were detected with a discriminatory power of the method of Ds = 0.96. The MLVA based on six VNTRs showed 29 profiles with a Ds = 0.90. The analysis of VF + AMR + MLVA data together showed 59 profiles with an increased discriminatory power (Ds = 0.98).This study highlights that the MLVA is recommended to add to other methodologies in order to study epidemiological relationships in this species Although within each dairy farm there was a predominance of certain serotypes/virulence profiles, the characteristics did not show total homogeneity, as expected due to the contagious nature of the pathogen. This suggests the incorporation of animals from other herds at some point, a practice not uncommon among dairy farms in Argentina. By other hand, the detection of a same clone in the same farm in different periods confirms that S. agalactiae strains can persist on dairy farms for a long time.
publishDate 2025
dc.date.none.fl_str_mv 2025-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/262839
Gerez, María Gabriela; Hernandez, Luciana Belén; Cadona, Jimena Soledad; Sanso, Andrea Mariel; Bustamante, Ana Victoria; Genetic diversity of Streptococcus agalactiae from dairy cattle with mastitis in Argentina; BioMed Central; BMC Veterinary Research; 21; 1; 5-2025; 1-11
1746-6148
CONICET Digital
CONICET
url http://hdl.handle.net/11336/262839
identifier_str_mv Gerez, María Gabriela; Hernandez, Luciana Belén; Cadona, Jimena Soledad; Sanso, Andrea Mariel; Bustamante, Ana Victoria; Genetic diversity of Streptococcus agalactiae from dairy cattle with mastitis in Argentina; BioMed Central; BMC Veterinary Research; 21; 1; 5-2025; 1-11
1746-6148
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1186/s12917-025-04584-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
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