Functional green-tuned proteorhodopsin from modern stromatolites

Autores
Albarracín, Virginia Helena; Kraiselburd, Ivana; Bamann, Christian; Wood, Phillip G.; Bamberg, Ernst; Farias, Maria Eugenia; Gärtner, Wolfgang
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The sequenced genome of the poly-extremophile Exiguobacterium sp. S17, isolated from modern stromatolites at Laguna Socompa (3,570 m), a High-Altitude Andean Lake (HAAL) in Argentinean Puna revealed a putative proteorhodopsin-encoding gene. The HAAL area is exposed to the highest UV irradiation on Earth, making the microbial community living in the stromatolites test cases for survival strategies under extreme conditions. The heterologous expressed protein E17R from Exiguobacterium (248 amino acids, 85% sequence identity to its ortholog ESR from E. sibiricum) was assembled with retinal displaying an absorbance maximum at 524 nm, which makes it a member of the green-absorbing PR-subfamily. Titration down to low pH values (eventually causing partial protein denaturation) indicated a pK value between two and three. Global fitting of data from laser flash-induced absorption changes gave evidence for an early red-shifted intermediate (its formation being below the experimental resolution) that decayed (τ1 =3.5μs) into another red-shifted intermediate. This species decayed in a two-step process (τ2 =84μs, τ3 = 11 ms), to which the initial state of E17-PR was reformed with a kinetics of 2 ms. Proton transport capability of the HAAL protein was determined by BLM measurements. Additional blue light irradiation reduced the proton current, clearly identifying a blue light absorbing, M-like intermediate. The apparent absence of this intermediate is explained by closely matching formation and decay kinetics.
Fil: Albarracín, Virginia Helena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina
Fil: Kraiselburd, Ivana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Bamann, Christian. Max Planck Institute for Biophysics; Alemania
Fil: Wood, Phillip G.. Max Planck Institute for Biophysics; Alemania
Fil: Bamberg, Ernst. Max Planck Institute for Biophysics; Alemania
Fil: Farias, Maria Eugenia. Max Planck Institute for Biophysics; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina
Fil: Gärtner, Wolfgang. Max Planck Institute for Chemical Energy Conversiom; Alemania
Materia
Exiguobacterium
Stromatolites
Laguna Socompa
Irradiation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/37774
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/37774
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Functional green-tuned proteorhodopsin from modern stromatolitesAlbarracín, Virginia HelenaKraiselburd, IvanaBamann, ChristianWood, Phillip G.Bamberg, ErnstFarias, Maria EugeniaGärtner, WolfgangExiguobacteriumStromatolitesLaguna SocompaIrradiationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The sequenced genome of the poly-extremophile Exiguobacterium sp. S17, isolated from modern stromatolites at Laguna Socompa (3,570 m), a High-Altitude Andean Lake (HAAL) in Argentinean Puna revealed a putative proteorhodopsin-encoding gene. The HAAL area is exposed to the highest UV irradiation on Earth, making the microbial community living in the stromatolites test cases for survival strategies under extreme conditions. The heterologous expressed protein E17R from Exiguobacterium (248 amino acids, 85% sequence identity to its ortholog ESR from E. sibiricum) was assembled with retinal displaying an absorbance maximum at 524 nm, which makes it a member of the green-absorbing PR-subfamily. Titration down to low pH values (eventually causing partial protein denaturation) indicated a pK value between two and three. Global fitting of data from laser flash-induced absorption changes gave evidence for an early red-shifted intermediate (its formation being below the experimental resolution) that decayed (τ1 =3.5μs) into another red-shifted intermediate. This species decayed in a two-step process (τ2 =84μs, τ3 = 11 ms), to which the initial state of E17-PR was reformed with a kinetics of 2 ms. Proton transport capability of the HAAL protein was determined by BLM measurements. Additional blue light irradiation reduced the proton current, clearly identifying a blue light absorbing, M-like intermediate. The apparent absence of this intermediate is explained by closely matching formation and decay kinetics.Fil: Albarracín, Virginia Helena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; ArgentinaFil: Kraiselburd, Ivana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Bamann, Christian. Max Planck Institute for Biophysics; AlemaniaFil: Wood, Phillip G.. Max Planck Institute for Biophysics; AlemaniaFil: Bamberg, Ernst. Max Planck Institute for Biophysics; AlemaniaFil: Farias, Maria Eugenia. Max Planck Institute for Biophysics; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; ArgentinaFil: Gärtner, Wolfgang. Max Planck Institute for Chemical Energy Conversiom; AlemaniaPublic Library of Science2016-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/37774Albarracín, Virginia Helena; Kraiselburd, Ivana; Bamann, Christian; Wood, Phillip G.; Bamberg, Ernst; et al.; Functional green-tuned proteorhodopsin from modern stromatolites; Public Library of Science; Plos One; 11; 5; 5-2016; 1-18; e01549621932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0154962info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0154962info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:31Zoai:ri.conicet.gov.ar:11336/37774instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:32.163CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Functional green-tuned proteorhodopsin from modern stromatolites
title Functional green-tuned proteorhodopsin from modern stromatolites
spellingShingle Functional green-tuned proteorhodopsin from modern stromatolites
Albarracín, Virginia Helena
Exiguobacterium
Stromatolites
Laguna Socompa
Irradiation
title_short Functional green-tuned proteorhodopsin from modern stromatolites
title_full Functional green-tuned proteorhodopsin from modern stromatolites
title_fullStr Functional green-tuned proteorhodopsin from modern stromatolites
title_full_unstemmed Functional green-tuned proteorhodopsin from modern stromatolites
title_sort Functional green-tuned proteorhodopsin from modern stromatolites
dc.creator.none.fl_str_mv Albarracín, Virginia Helena
Kraiselburd, Ivana
Bamann, Christian
Wood, Phillip G.
Bamberg, Ernst
Farias, Maria Eugenia
Gärtner, Wolfgang
author Albarracín, Virginia Helena
author_facet Albarracín, Virginia Helena
Kraiselburd, Ivana
Bamann, Christian
Wood, Phillip G.
Bamberg, Ernst
Farias, Maria Eugenia
Gärtner, Wolfgang
author_role author
author2 Kraiselburd, Ivana
Bamann, Christian
Wood, Phillip G.
Bamberg, Ernst
Farias, Maria Eugenia
Gärtner, Wolfgang
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Exiguobacterium
Stromatolites
Laguna Socompa
Irradiation
topic Exiguobacterium
Stromatolites
Laguna Socompa
Irradiation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The sequenced genome of the poly-extremophile Exiguobacterium sp. S17, isolated from modern stromatolites at Laguna Socompa (3,570 m), a High-Altitude Andean Lake (HAAL) in Argentinean Puna revealed a putative proteorhodopsin-encoding gene. The HAAL area is exposed to the highest UV irradiation on Earth, making the microbial community living in the stromatolites test cases for survival strategies under extreme conditions. The heterologous expressed protein E17R from Exiguobacterium (248 amino acids, 85% sequence identity to its ortholog ESR from E. sibiricum) was assembled with retinal displaying an absorbance maximum at 524 nm, which makes it a member of the green-absorbing PR-subfamily. Titration down to low pH values (eventually causing partial protein denaturation) indicated a pK value between two and three. Global fitting of data from laser flash-induced absorption changes gave evidence for an early red-shifted intermediate (its formation being below the experimental resolution) that decayed (τ1 =3.5μs) into another red-shifted intermediate. This species decayed in a two-step process (τ2 =84μs, τ3 = 11 ms), to which the initial state of E17-PR was reformed with a kinetics of 2 ms. Proton transport capability of the HAAL protein was determined by BLM measurements. Additional blue light irradiation reduced the proton current, clearly identifying a blue light absorbing, M-like intermediate. The apparent absence of this intermediate is explained by closely matching formation and decay kinetics.
Fil: Albarracín, Virginia Helena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina
Fil: Kraiselburd, Ivana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Bamann, Christian. Max Planck Institute for Biophysics; Alemania
Fil: Wood, Phillip G.. Max Planck Institute for Biophysics; Alemania
Fil: Bamberg, Ernst. Max Planck Institute for Biophysics; Alemania
Fil: Farias, Maria Eugenia. Max Planck Institute for Biophysics; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina
Fil: Gärtner, Wolfgang. Max Planck Institute for Chemical Energy Conversiom; Alemania
description The sequenced genome of the poly-extremophile Exiguobacterium sp. S17, isolated from modern stromatolites at Laguna Socompa (3,570 m), a High-Altitude Andean Lake (HAAL) in Argentinean Puna revealed a putative proteorhodopsin-encoding gene. The HAAL area is exposed to the highest UV irradiation on Earth, making the microbial community living in the stromatolites test cases for survival strategies under extreme conditions. The heterologous expressed protein E17R from Exiguobacterium (248 amino acids, 85% sequence identity to its ortholog ESR from E. sibiricum) was assembled with retinal displaying an absorbance maximum at 524 nm, which makes it a member of the green-absorbing PR-subfamily. Titration down to low pH values (eventually causing partial protein denaturation) indicated a pK value between two and three. Global fitting of data from laser flash-induced absorption changes gave evidence for an early red-shifted intermediate (its formation being below the experimental resolution) that decayed (τ1 =3.5μs) into another red-shifted intermediate. This species decayed in a two-step process (τ2 =84μs, τ3 = 11 ms), to which the initial state of E17-PR was reformed with a kinetics of 2 ms. Proton transport capability of the HAAL protein was determined by BLM measurements. Additional blue light irradiation reduced the proton current, clearly identifying a blue light absorbing, M-like intermediate. The apparent absence of this intermediate is explained by closely matching formation and decay kinetics.
publishDate 2016
dc.date.none.fl_str_mv 2016-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/37774
Albarracín, Virginia Helena; Kraiselburd, Ivana; Bamann, Christian; Wood, Phillip G.; Bamberg, Ernst; et al.; Functional green-tuned proteorhodopsin from modern stromatolites; Public Library of Science; Plos One; 11; 5; 5-2016; 1-18; e0154962
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/37774
identifier_str_mv Albarracín, Virginia Helena; Kraiselburd, Ivana; Bamann, Christian; Wood, Phillip G.; Bamberg, Ernst; et al.; Functional green-tuned proteorhodopsin from modern stromatolites; Public Library of Science; Plos One; 11; 5; 5-2016; 1-18; e0154962
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0154962
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0154962
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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