Action of Ketamine on acquisition in the novel object recognition test and the metabolic activity in hippocampus

Autores
Guevara, Manuel Alejandro; Romanowicz, Esteban Alejandro; García Menéndez, Sebastián Marcelo Manuel; Lafuente, José Vicente; Baiardi, Gustavo Carlos; Gargiulo, Pascual Angel
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Psychotogenic effects of ketamine, due to antagonism of NMDA (N-methyl-D-aspartic acid) glutamate receptors, are widely known, and they are subject of study in our laboratory. In the present study we searched for the effect of systemic administration of ketamine at low doses in the novel object recognition test. Holtzman derived colony rats weighing 240-290 g were used. Novel object recognition test involves variables related to memory and perception. We used an intraperitoneal administration of ketamine in sub-anesthetic doses (1.25, 2.5 and 5 mg / kg ip) 3 min before training. Test was realized 120 min after training. Additionally, groups of five rats treated with saline or different sub-anesthetic doses were paired for measurement of hippocampus metabolic activity 8 min after injection. It was evaluated using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) through spectrophotometric wavelength measurement. We observed that treatment produced a significant increase in total scan time in the evaluation session (p <0.05) at all doses. It also led to a significant decrease in the discrimination rate (p <0.001) at doses of 2.5 and 5 mg/kg. These findings allow us to postulate that treatment induced an inhibitory effect on short-term memory. Metabolic activity showed an evident and significant decrease in hippocampus (1.25 mg/kg; 2.5 mg/kg, p<0.001). We conclude that systemically administered ketamine in low doses produces inhibitory effect on short-term memory. These behavioral findings are accompanied by a decrease in metabolic activity in the hippocampus.
Fil: Guevara, Manuel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Laboratorio de Neurociencias y Psicologia Experimental ; Facultad de Ciencias Medicas ; Universidad Nacional de Cuyo;
Fil: Romanowicz, Esteban Alejandro. Laboratorio de Neurociencias y Psicologia Experimental ; Facultad de Ciencias Medicas ; Universidad Nacional de Cuyo;
Fil: García Menéndez, Sebastián Marcelo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Laboratorio de Neurociencias y Psicologia Experimental ; Facultad de Ciencias Medicas ; Universidad Nacional de Cuyo;
Fil: Lafuente, José Vicente. Universidad del País Vasco; España
Fil: Baiardi, Gustavo Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Laboratorio de Neurociencias y Psicologia Experimental ; Facultad de Ciencias Medicas ; Universidad Nacional de Cuyo;
Fil: Gargiulo, Pascual Angel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Laboratorio de Neurociencias y Psicologia Experimental ; Facultad de Ciencias Medicas ; Universidad Nacional de Cuyo;
XXXVI Reunión Anual de la Sociedad de Biología de Cuyo
Mendoza
Argentina
Sociedad de Biología de Cuyo.
Materia
KETAMINE
MEMORY
HIPPOCAMPUS
RAT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/241033
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/241033
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Action of Ketamine on acquisition in the novel object recognition test and the metabolic activity in hippocampusGuevara, Manuel AlejandroRomanowicz, Esteban AlejandroGarcía Menéndez, Sebastián Marcelo ManuelLafuente, José VicenteBaiardi, Gustavo CarlosGargiulo, Pascual AngelKETAMINEMEMORYHIPPOCAMPUSRAThttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Psychotogenic effects of ketamine, due to antagonism of NMDA (N-methyl-D-aspartic acid) glutamate receptors, are widely known, and they are subject of study in our laboratory. In the present study we searched for the effect of systemic administration of ketamine at low doses in the novel object recognition test. Holtzman derived colony rats weighing 240-290 g were used. Novel object recognition test involves variables related to memory and perception. We used an intraperitoneal administration of ketamine in sub-anesthetic doses (1.25, 2.5 and 5 mg / kg ip) 3 min before training. Test was realized 120 min after training. Additionally, groups of five rats treated with saline or different sub-anesthetic doses were paired for measurement of hippocampus metabolic activity 8 min after injection. It was evaluated using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) through spectrophotometric wavelength measurement. We observed that treatment produced a significant increase in total scan time in the evaluation session (p <0.05) at all doses. It also led to a significant decrease in the discrimination rate (p <0.001) at doses of 2.5 and 5 mg/kg. These findings allow us to postulate that treatment induced an inhibitory effect on short-term memory. Metabolic activity showed an evident and significant decrease in hippocampus (1.25 mg/kg; 2.5 mg/kg, p<0.001). We conclude that systemically administered ketamine in low doses produces inhibitory effect on short-term memory. These behavioral findings are accompanied by a decrease in metabolic activity in the hippocampus.Fil: Guevara, Manuel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Laboratorio de Neurociencias y Psicologia Experimental ; Facultad de Ciencias Medicas ; Universidad Nacional de Cuyo;Fil: Romanowicz, Esteban Alejandro. Laboratorio de Neurociencias y Psicologia Experimental ; Facultad de Ciencias Medicas ; Universidad Nacional de Cuyo;Fil: García Menéndez, Sebastián Marcelo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Laboratorio de Neurociencias y Psicologia Experimental ; Facultad de Ciencias Medicas ; Universidad Nacional de Cuyo;Fil: Lafuente, José Vicente. Universidad del País Vasco; EspañaFil: Baiardi, Gustavo Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Laboratorio de Neurociencias y Psicologia Experimental ; Facultad de Ciencias Medicas ; Universidad Nacional de Cuyo;Fil: Gargiulo, Pascual Angel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Laboratorio de Neurociencias y Psicologia Experimental ; Facultad de Ciencias Medicas ; Universidad Nacional de Cuyo;XXXVI Reunión Anual de la Sociedad de Biología de CuyoMendozaArgentinaSociedad de Biología de Cuyo.Tech Science Press2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/241033Action of Ketamine on acquisition in the novel object recognition test and the metabolic activity in hippocampus; XXXVI Reunión Anual de la Sociedad de Biología de Cuyo; Mendoza; Argentina; 2018; 71-710327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/wp-content/uploads/2019/08/Biocell-2018.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:17:47Zoai:ri.conicet.gov.ar:11336/241033instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:17:47.308CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Action of Ketamine on acquisition in the novel object recognition test and the metabolic activity in hippocampus
title Action of Ketamine on acquisition in the novel object recognition test and the metabolic activity in hippocampus
spellingShingle Action of Ketamine on acquisition in the novel object recognition test and the metabolic activity in hippocampus
Guevara, Manuel Alejandro
KETAMINE
MEMORY
HIPPOCAMPUS
RAT
title_short Action of Ketamine on acquisition in the novel object recognition test and the metabolic activity in hippocampus
title_full Action of Ketamine on acquisition in the novel object recognition test and the metabolic activity in hippocampus
title_fullStr Action of Ketamine on acquisition in the novel object recognition test and the metabolic activity in hippocampus
title_full_unstemmed Action of Ketamine on acquisition in the novel object recognition test and the metabolic activity in hippocampus
title_sort Action of Ketamine on acquisition in the novel object recognition test and the metabolic activity in hippocampus
dc.creator.none.fl_str_mv Guevara, Manuel Alejandro
Romanowicz, Esteban Alejandro
García Menéndez, Sebastián Marcelo Manuel
Lafuente, José Vicente
Baiardi, Gustavo Carlos
Gargiulo, Pascual Angel
author Guevara, Manuel Alejandro
author_facet Guevara, Manuel Alejandro
Romanowicz, Esteban Alejandro
García Menéndez, Sebastián Marcelo Manuel
Lafuente, José Vicente
Baiardi, Gustavo Carlos
Gargiulo, Pascual Angel
author_role author
author2 Romanowicz, Esteban Alejandro
García Menéndez, Sebastián Marcelo Manuel
Lafuente, José Vicente
Baiardi, Gustavo Carlos
Gargiulo, Pascual Angel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv KETAMINE
MEMORY
HIPPOCAMPUS
RAT
topic KETAMINE
MEMORY
HIPPOCAMPUS
RAT
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Psychotogenic effects of ketamine, due to antagonism of NMDA (N-methyl-D-aspartic acid) glutamate receptors, are widely known, and they are subject of study in our laboratory. In the present study we searched for the effect of systemic administration of ketamine at low doses in the novel object recognition test. Holtzman derived colony rats weighing 240-290 g were used. Novel object recognition test involves variables related to memory and perception. We used an intraperitoneal administration of ketamine in sub-anesthetic doses (1.25, 2.5 and 5 mg / kg ip) 3 min before training. Test was realized 120 min after training. Additionally, groups of five rats treated with saline or different sub-anesthetic doses were paired for measurement of hippocampus metabolic activity 8 min after injection. It was evaluated using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) through spectrophotometric wavelength measurement. We observed that treatment produced a significant increase in total scan time in the evaluation session (p <0.05) at all doses. It also led to a significant decrease in the discrimination rate (p <0.001) at doses of 2.5 and 5 mg/kg. These findings allow us to postulate that treatment induced an inhibitory effect on short-term memory. Metabolic activity showed an evident and significant decrease in hippocampus (1.25 mg/kg; 2.5 mg/kg, p<0.001). We conclude that systemically administered ketamine in low doses produces inhibitory effect on short-term memory. These behavioral findings are accompanied by a decrease in metabolic activity in the hippocampus.
Fil: Guevara, Manuel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Laboratorio de Neurociencias y Psicologia Experimental ; Facultad de Ciencias Medicas ; Universidad Nacional de Cuyo;
Fil: Romanowicz, Esteban Alejandro. Laboratorio de Neurociencias y Psicologia Experimental ; Facultad de Ciencias Medicas ; Universidad Nacional de Cuyo;
Fil: García Menéndez, Sebastián Marcelo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Laboratorio de Neurociencias y Psicologia Experimental ; Facultad de Ciencias Medicas ; Universidad Nacional de Cuyo;
Fil: Lafuente, José Vicente. Universidad del País Vasco; España
Fil: Baiardi, Gustavo Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Laboratorio de Neurociencias y Psicologia Experimental ; Facultad de Ciencias Medicas ; Universidad Nacional de Cuyo;
Fil: Gargiulo, Pascual Angel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Laboratorio de Neurociencias y Psicologia Experimental ; Facultad de Ciencias Medicas ; Universidad Nacional de Cuyo;
XXXVI Reunión Anual de la Sociedad de Biología de Cuyo
Mendoza
Argentina
Sociedad de Biología de Cuyo.
description Psychotogenic effects of ketamine, due to antagonism of NMDA (N-methyl-D-aspartic acid) glutamate receptors, are widely known, and they are subject of study in our laboratory. In the present study we searched for the effect of systemic administration of ketamine at low doses in the novel object recognition test. Holtzman derived colony rats weighing 240-290 g were used. Novel object recognition test involves variables related to memory and perception. We used an intraperitoneal administration of ketamine in sub-anesthetic doses (1.25, 2.5 and 5 mg / kg ip) 3 min before training. Test was realized 120 min after training. Additionally, groups of five rats treated with saline or different sub-anesthetic doses were paired for measurement of hippocampus metabolic activity 8 min after injection. It was evaluated using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) through spectrophotometric wavelength measurement. We observed that treatment produced a significant increase in total scan time in the evaluation session (p <0.05) at all doses. It also led to a significant decrease in the discrimination rate (p <0.001) at doses of 2.5 and 5 mg/kg. These findings allow us to postulate that treatment induced an inhibitory effect on short-term memory. Metabolic activity showed an evident and significant decrease in hippocampus (1.25 mg/kg; 2.5 mg/kg, p<0.001). We conclude that systemically administered ketamine in low doses produces inhibitory effect on short-term memory. These behavioral findings are accompanied by a decrease in metabolic activity in the hippocampus.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/241033
Action of Ketamine on acquisition in the novel object recognition test and the metabolic activity in hippocampus; XXXVI Reunión Anual de la Sociedad de Biología de Cuyo; Mendoza; Argentina; 2018; 71-71
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/241033
identifier_str_mv Action of Ketamine on acquisition in the novel object recognition test and the metabolic activity in hippocampus; XXXVI Reunión Anual de la Sociedad de Biología de Cuyo; Mendoza; Argentina; 2018; 71-71
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/wp-content/uploads/2019/08/Biocell-2018.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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