Identification of an Arabidopsis mitoferrinlike carrier protein involved in Fe metabolism

Autores
Tarantino, Delia; Morandini, Piero; Ramírez, Leonor; Soave, Carlo; Murgia, Irene
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Iron has a major role in mitochondrial as well as in chloroplast metabolism, however the processes involved in organelle iron transport in plants are only partially understood. To identify mitochondrial iron transporters in Arabidopsis, we searched for proteins homologous to the Danio rerio (zebrafish) Mitoferrin2 MFRN2, a mitochondrial iron importer in non-erythroid cells. Among the identified putative Arabidopsis mitoferrinlike proteins, we focused on that one encoded by At5g42130, which we named AtMfl1 (MitoFerrinLike1). AtMfl1 expression strongly correlates with genes coding for proteins involved in chloroplast metabolism. Such an unexpected result is supported by the identification by different research groups, of the protein encoded by At5g42130 and of its homologs from various plant species in the inner chloroplastic envelope membrane proteome. Notably, neither the protein encoded by At5g42130 nor its homologs from other plant species have been identified in the mitochondrial proteome. AtMfl1 gene expression is dependent on Fe supply: AtMfl1 transcript strongly accumulates under Fe excess, moderately under Fe sufficiency and weakly under Fe deficiency. In order to understand the physiological role of AtMfl1, we isolated and characterized two independent AtMfl1 KO mutants, atmfl1-1 and atmfl1-2: both show reduced vegetative growth. When grown under conditions of Fe excess, atmfl1-1 and atmfl1-2 mutants (seedlings, rosette leaves) contain less total Fe than wt and also reduced expression of the iron storage ferritin AtFer1. Taken together, these results suggest that Arabidopsis mitoferrinlike gene AtMfl1 is involved in Fe transport into chloroplasts, under different conditions of Fe supply and that suppression of its expression alters plant Fe accumulation in various developmental stages.
Fil: Tarantino, Delia. Universita Degli Studi Di Milano; Italia
Fil: Morandini, Piero. Universita Degli Studi Di Milano; Italia
Fil: Ramírez, Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universita Degli Studi Di Milano; Italia. Universidad Nacional de Mar del Plata; Argentina
Fil: Soave, Carlo. Universita Degli Studi Di Milano; Italia
Fil: Murgia, Irene. Universita Degli Studi Di Milano; Italia
Fuente
15th International Symposium on Iron Nutrition and Interactions in Plants
Materia
Arabidopsis
Carriers
Chloroplasts
Correlation Analysis
Iron
Mitochondria
Mitoferrin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13063
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/13063
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Identification of an Arabidopsis mitoferrinlike carrier protein involved in Fe metabolismTarantino, DeliaMorandini, PieroRamírez, LeonorSoave, CarloMurgia, IreneArabidopsisCarriersChloroplastsCorrelation AnalysisIronMitochondriaMitoferrinhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Iron has a major role in mitochondrial as well as in chloroplast metabolism, however the processes involved in organelle iron transport in plants are only partially understood. To identify mitochondrial iron transporters in Arabidopsis, we searched for proteins homologous to the Danio rerio (zebrafish) Mitoferrin2 MFRN2, a mitochondrial iron importer in non-erythroid cells. Among the identified putative Arabidopsis mitoferrinlike proteins, we focused on that one encoded by At5g42130, which we named AtMfl1 (MitoFerrinLike1). AtMfl1 expression strongly correlates with genes coding for proteins involved in chloroplast metabolism. Such an unexpected result is supported by the identification by different research groups, of the protein encoded by At5g42130 and of its homologs from various plant species in the inner chloroplastic envelope membrane proteome. Notably, neither the protein encoded by At5g42130 nor its homologs from other plant species have been identified in the mitochondrial proteome. AtMfl1 gene expression is dependent on Fe supply: AtMfl1 transcript strongly accumulates under Fe excess, moderately under Fe sufficiency and weakly under Fe deficiency. In order to understand the physiological role of AtMfl1, we isolated and characterized two independent AtMfl1 KO mutants, atmfl1-1 and atmfl1-2: both show reduced vegetative growth. When grown under conditions of Fe excess, atmfl1-1 and atmfl1-2 mutants (seedlings, rosette leaves) contain less total Fe than wt and also reduced expression of the iron storage ferritin AtFer1. Taken together, these results suggest that Arabidopsis mitoferrinlike gene AtMfl1 is involved in Fe transport into chloroplasts, under different conditions of Fe supply and that suppression of its expression alters plant Fe accumulation in various developmental stages.Fil: Tarantino, Delia. Universita Degli Studi Di Milano; ItaliaFil: Morandini, Piero. Universita Degli Studi Di Milano; ItaliaFil: Ramírez, Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universita Degli Studi Di Milano; Italia. Universidad Nacional de Mar del Plata; ArgentinaFil: Soave, Carlo. Universita Degli Studi Di Milano; ItaliaFil: Murgia, Irene. Universita Degli Studi Di Milano; ItaliaElsevier Masson2011-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13063Tarantino, Delia; Morandini, Piero; Ramírez, Leonor; Soave, Carlo; Murgia, Irene; Identification of an Arabidopsis mitoferrinlike carrier protein involved in Fe metabolism; Elsevier Masson; Plant Physiology And Biochemistry; 49; 5; 5-2011; 520-5290981-942815th International Symposium on Iron Nutrition and Interactions in Plantsreponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicasenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0981942811000477info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1016/j.plaphy.2011.02.003info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/2025-09-29T09:39:44Zoai:ri.conicet.gov.ar:11336/13063instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:39:45.039CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Identification of an Arabidopsis mitoferrinlike carrier protein involved in Fe metabolism
title Identification of an Arabidopsis mitoferrinlike carrier protein involved in Fe metabolism
spellingShingle Identification of an Arabidopsis mitoferrinlike carrier protein involved in Fe metabolism
Tarantino, Delia
Arabidopsis
Carriers
Chloroplasts
Correlation Analysis
Iron
Mitochondria
Mitoferrin
title_short Identification of an Arabidopsis mitoferrinlike carrier protein involved in Fe metabolism
title_full Identification of an Arabidopsis mitoferrinlike carrier protein involved in Fe metabolism
title_fullStr Identification of an Arabidopsis mitoferrinlike carrier protein involved in Fe metabolism
title_full_unstemmed Identification of an Arabidopsis mitoferrinlike carrier protein involved in Fe metabolism
title_sort Identification of an Arabidopsis mitoferrinlike carrier protein involved in Fe metabolism
dc.creator.none.fl_str_mv Tarantino, Delia
Morandini, Piero
Ramírez, Leonor
Soave, Carlo
Murgia, Irene
author Tarantino, Delia
author_facet Tarantino, Delia
Morandini, Piero
Ramírez, Leonor
Soave, Carlo
Murgia, Irene
author_role author
author2 Morandini, Piero
Ramírez, Leonor
Soave, Carlo
Murgia, Irene
author2_role author
author
author
author
dc.subject.none.fl_str_mv Arabidopsis
Carriers
Chloroplasts
Correlation Analysis
Iron
Mitochondria
Mitoferrin
topic Arabidopsis
Carriers
Chloroplasts
Correlation Analysis
Iron
Mitochondria
Mitoferrin
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Iron has a major role in mitochondrial as well as in chloroplast metabolism, however the processes involved in organelle iron transport in plants are only partially understood. To identify mitochondrial iron transporters in Arabidopsis, we searched for proteins homologous to the Danio rerio (zebrafish) Mitoferrin2 MFRN2, a mitochondrial iron importer in non-erythroid cells. Among the identified putative Arabidopsis mitoferrinlike proteins, we focused on that one encoded by At5g42130, which we named AtMfl1 (MitoFerrinLike1). AtMfl1 expression strongly correlates with genes coding for proteins involved in chloroplast metabolism. Such an unexpected result is supported by the identification by different research groups, of the protein encoded by At5g42130 and of its homologs from various plant species in the inner chloroplastic envelope membrane proteome. Notably, neither the protein encoded by At5g42130 nor its homologs from other plant species have been identified in the mitochondrial proteome. AtMfl1 gene expression is dependent on Fe supply: AtMfl1 transcript strongly accumulates under Fe excess, moderately under Fe sufficiency and weakly under Fe deficiency. In order to understand the physiological role of AtMfl1, we isolated and characterized two independent AtMfl1 KO mutants, atmfl1-1 and atmfl1-2: both show reduced vegetative growth. When grown under conditions of Fe excess, atmfl1-1 and atmfl1-2 mutants (seedlings, rosette leaves) contain less total Fe than wt and also reduced expression of the iron storage ferritin AtFer1. Taken together, these results suggest that Arabidopsis mitoferrinlike gene AtMfl1 is involved in Fe transport into chloroplasts, under different conditions of Fe supply and that suppression of its expression alters plant Fe accumulation in various developmental stages.
Fil: Tarantino, Delia. Universita Degli Studi Di Milano; Italia
Fil: Morandini, Piero. Universita Degli Studi Di Milano; Italia
Fil: Ramírez, Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universita Degli Studi Di Milano; Italia. Universidad Nacional de Mar del Plata; Argentina
Fil: Soave, Carlo. Universita Degli Studi Di Milano; Italia
Fil: Murgia, Irene. Universita Degli Studi Di Milano; Italia
description Iron has a major role in mitochondrial as well as in chloroplast metabolism, however the processes involved in organelle iron transport in plants are only partially understood. To identify mitochondrial iron transporters in Arabidopsis, we searched for proteins homologous to the Danio rerio (zebrafish) Mitoferrin2 MFRN2, a mitochondrial iron importer in non-erythroid cells. Among the identified putative Arabidopsis mitoferrinlike proteins, we focused on that one encoded by At5g42130, which we named AtMfl1 (MitoFerrinLike1). AtMfl1 expression strongly correlates with genes coding for proteins involved in chloroplast metabolism. Such an unexpected result is supported by the identification by different research groups, of the protein encoded by At5g42130 and of its homologs from various plant species in the inner chloroplastic envelope membrane proteome. Notably, neither the protein encoded by At5g42130 nor its homologs from other plant species have been identified in the mitochondrial proteome. AtMfl1 gene expression is dependent on Fe supply: AtMfl1 transcript strongly accumulates under Fe excess, moderately under Fe sufficiency and weakly under Fe deficiency. In order to understand the physiological role of AtMfl1, we isolated and characterized two independent AtMfl1 KO mutants, atmfl1-1 and atmfl1-2: both show reduced vegetative growth. When grown under conditions of Fe excess, atmfl1-1 and atmfl1-2 mutants (seedlings, rosette leaves) contain less total Fe than wt and also reduced expression of the iron storage ferritin AtFer1. Taken together, these results suggest that Arabidopsis mitoferrinlike gene AtMfl1 is involved in Fe transport into chloroplasts, under different conditions of Fe supply and that suppression of its expression alters plant Fe accumulation in various developmental stages.
publishDate 2011
dc.date.none.fl_str_mv 2011-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13063
Tarantino, Delia; Morandini, Piero; Ramírez, Leonor; Soave, Carlo; Murgia, Irene; Identification of an Arabidopsis mitoferrinlike carrier protein involved in Fe metabolism; Elsevier Masson; Plant Physiology And Biochemistry; 49; 5; 5-2011; 520-529
0981-9428
url http://hdl.handle.net/11336/13063
identifier_str_mv Tarantino, Delia; Morandini, Piero; Ramírez, Leonor; Soave, Carlo; Murgia, Irene; Identification of an Arabidopsis mitoferrinlike carrier protein involved in Fe metabolism; Elsevier Masson; Plant Physiology And Biochemistry; 49; 5; 5-2011; 520-529
0981-9428
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0981942811000477
info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1016/j.plaphy.2011.02.003
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Masson
publisher.none.fl_str_mv Elsevier Masson
dc.source.none.fl_str_mv 15th International Symposium on Iron Nutrition and Interactions in Plants
reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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