Activation of PPAR alfa and PPAR beta/delta regulates Sertoli cell metabolism.

Autores
Regueira, Mariana; Riera, Maria Fernanda; Galardo, Maria Noel Lujan; Pellizzari, Eliana Herminia; Cigorraga, Selva Beatriz; Meroni, Silvina Beatriz
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The purpose of this study was to evaluate the existence of a possible simultaneous regulation of fatty acid (FA) metabolism and lactate production by PPAR a and PPAR b/d activation in Sertoli cells (SC). SC cultures obtained from 20-day-old rats were incubated with WY14643 or GW0742-pharmacological activators of PPAR alfa and PPAR beta/delta respectively. The fatty acid transporter CD36, carnitine palmitoyltransferase 1, long- and medium-chain 3-hydroxyacyl-CoA dehydrogenases mRNA levels were analyzed. An increase in the above-mentioned genes in response to activation of both nuclear receptors was observed. Additionally, PPAR b/d activation increased lactate production as a consequence of increased pyruvate availability by inhibiting the Pyruvate Dehydrogenase Complex. Altogether, these results suggest that in SC, PPAR a activation participates in the regulation of FA metabolism. On the other hand, PPAR b/d activation regulates FA metabolism and lactate production ensuring simultaneously the energetic metabolism for SC and germ cells.
Fil: Regueira, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
Fil: Riera, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
Fil: Galardo, Maria Noel Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
Fil: Pellizzari, Eliana Herminia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
Fil: Cigorraga, Selva Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
Fil: Meroni, Silvina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
Materia
Sertoli
Metabolismo Energetico
Ppar
Lactato
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8191
Acceder
id CONICETDig_143d93b990c4bd42638895800916b078
oai_identifier_str oai:ri.conicet.gov.ar:11336/8191
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network_name_str CONICET Digital (CONICET)
spelling Activation of PPAR alfa and PPAR beta/delta regulates Sertoli cell metabolism.Regueira, MarianaRiera, Maria FernandaGalardo, Maria Noel LujanPellizzari, Eliana HerminiaCigorraga, Selva BeatrizMeroni, Silvina BeatrizSertoliMetabolismo EnergeticoPparLactatohttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The purpose of this study was to evaluate the existence of a possible simultaneous regulation of fatty acid (FA) metabolism and lactate production by PPAR a and PPAR b/d activation in Sertoli cells (SC). SC cultures obtained from 20-day-old rats were incubated with WY14643 or GW0742-pharmacological activators of PPAR alfa and PPAR beta/delta respectively. The fatty acid transporter CD36, carnitine palmitoyltransferase 1, long- and medium-chain 3-hydroxyacyl-CoA dehydrogenases mRNA levels were analyzed. An increase in the above-mentioned genes in response to activation of both nuclear receptors was observed. Additionally, PPAR b/d activation increased lactate production as a consequence of increased pyruvate availability by inhibiting the Pyruvate Dehydrogenase Complex. Altogether, these results suggest that in SC, PPAR a activation participates in the regulation of FA metabolism. On the other hand, PPAR b/d activation regulates FA metabolism and lactate production ensuring simultaneously the energetic metabolism for SC and germ cells.Fil: Regueira, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; ArgentinaFil: Riera, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; ArgentinaFil: Galardo, Maria Noel Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; ArgentinaFil: Pellizzari, Eliana Herminia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; ArgentinaFil: Cigorraga, Selva Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; ArgentinaFil: Meroni, Silvina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; ArgentinaElsevier Ireland2014-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8191Regueira, Mariana; Riera, Maria Fernanda; Galardo, Maria Noel Lujan; Pellizzari, Eliana Herminia; Cigorraga, Selva Beatriz; et al.; Activation of PPAR alfa and PPAR beta/delta regulates Sertoli cell metabolism.; Elsevier Ireland; Molecular And Cellular Endocrinology; 382; 1; 1-2014; 271-2810303-7207enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0303720713004462info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mce.2013.10.006info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:45:18Zoai:ri.conicet.gov.ar:11336/8191instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:45:18.664CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Activation of PPAR alfa and PPAR beta/delta regulates Sertoli cell metabolism.
title Activation of PPAR alfa and PPAR beta/delta regulates Sertoli cell metabolism.
spellingShingle Activation of PPAR alfa and PPAR beta/delta regulates Sertoli cell metabolism.
Regueira, Mariana
Sertoli
Metabolismo Energetico
Ppar
Lactato
title_short Activation of PPAR alfa and PPAR beta/delta regulates Sertoli cell metabolism.
title_full Activation of PPAR alfa and PPAR beta/delta regulates Sertoli cell metabolism.
title_fullStr Activation of PPAR alfa and PPAR beta/delta regulates Sertoli cell metabolism.
title_full_unstemmed Activation of PPAR alfa and PPAR beta/delta regulates Sertoli cell metabolism.
title_sort Activation of PPAR alfa and PPAR beta/delta regulates Sertoli cell metabolism.
dc.creator.none.fl_str_mv Regueira, Mariana
Riera, Maria Fernanda
Galardo, Maria Noel Lujan
Pellizzari, Eliana Herminia
Cigorraga, Selva Beatriz
Meroni, Silvina Beatriz
author Regueira, Mariana
author_facet Regueira, Mariana
Riera, Maria Fernanda
Galardo, Maria Noel Lujan
Pellizzari, Eliana Herminia
Cigorraga, Selva Beatriz
Meroni, Silvina Beatriz
author_role author
author2 Riera, Maria Fernanda
Galardo, Maria Noel Lujan
Pellizzari, Eliana Herminia
Cigorraga, Selva Beatriz
Meroni, Silvina Beatriz
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Sertoli
Metabolismo Energetico
Ppar
Lactato
topic Sertoli
Metabolismo Energetico
Ppar
Lactato
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The purpose of this study was to evaluate the existence of a possible simultaneous regulation of fatty acid (FA) metabolism and lactate production by PPAR a and PPAR b/d activation in Sertoli cells (SC). SC cultures obtained from 20-day-old rats were incubated with WY14643 or GW0742-pharmacological activators of PPAR alfa and PPAR beta/delta respectively. The fatty acid transporter CD36, carnitine palmitoyltransferase 1, long- and medium-chain 3-hydroxyacyl-CoA dehydrogenases mRNA levels were analyzed. An increase in the above-mentioned genes in response to activation of both nuclear receptors was observed. Additionally, PPAR b/d activation increased lactate production as a consequence of increased pyruvate availability by inhibiting the Pyruvate Dehydrogenase Complex. Altogether, these results suggest that in SC, PPAR a activation participates in the regulation of FA metabolism. On the other hand, PPAR b/d activation regulates FA metabolism and lactate production ensuring simultaneously the energetic metabolism for SC and germ cells.
Fil: Regueira, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
Fil: Riera, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
Fil: Galardo, Maria Noel Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
Fil: Pellizzari, Eliana Herminia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
Fil: Cigorraga, Selva Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
Fil: Meroni, Silvina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas; Argentina
description The purpose of this study was to evaluate the existence of a possible simultaneous regulation of fatty acid (FA) metabolism and lactate production by PPAR a and PPAR b/d activation in Sertoli cells (SC). SC cultures obtained from 20-day-old rats were incubated with WY14643 or GW0742-pharmacological activators of PPAR alfa and PPAR beta/delta respectively. The fatty acid transporter CD36, carnitine palmitoyltransferase 1, long- and medium-chain 3-hydroxyacyl-CoA dehydrogenases mRNA levels were analyzed. An increase in the above-mentioned genes in response to activation of both nuclear receptors was observed. Additionally, PPAR b/d activation increased lactate production as a consequence of increased pyruvate availability by inhibiting the Pyruvate Dehydrogenase Complex. Altogether, these results suggest that in SC, PPAR a activation participates in the regulation of FA metabolism. On the other hand, PPAR b/d activation regulates FA metabolism and lactate production ensuring simultaneously the energetic metabolism for SC and germ cells.
publishDate 2014
dc.date.none.fl_str_mv 2014-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8191
Regueira, Mariana; Riera, Maria Fernanda; Galardo, Maria Noel Lujan; Pellizzari, Eliana Herminia; Cigorraga, Selva Beatriz; et al.; Activation of PPAR alfa and PPAR beta/delta regulates Sertoli cell metabolism.; Elsevier Ireland; Molecular And Cellular Endocrinology; 382; 1; 1-2014; 271-281
0303-7207
url http://hdl.handle.net/11336/8191
identifier_str_mv Regueira, Mariana; Riera, Maria Fernanda; Galardo, Maria Noel Lujan; Pellizzari, Eliana Herminia; Cigorraga, Selva Beatriz; et al.; Activation of PPAR alfa and PPAR beta/delta regulates Sertoli cell metabolism.; Elsevier Ireland; Molecular And Cellular Endocrinology; 382; 1; 1-2014; 271-281
0303-7207
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0303720713004462
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mce.2013.10.006
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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