Impact of osmotic/matric stress and heat shock on environmental tolerance induction of bacterial biocontrol agents against Fusarium verticillioides

Autores
Sartori, Melina Victoria; Nesci, Andrea Verónica; Etcheverry, Miriam Graciela
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bacillus amyloliquefaciens and Microbacterium oleovorans reduced Fusarium verticillioides count and decreased significantly fumonisins B1 and B2 levels in maize grains. The aims of this study were to determine the effect of water stress tolerance and heat-shock survival in cells of the biocontrol agents B. amyloliquefaciens and M. oleovorans. The aw of solid and liquid media, tryptic soy medium, was modified to 0.99, 0.98, 0.97 and 0.96 by the addition of the ionic solute NaCl and with the non-ionic solutes such as glycerol and glucose. The non-ionic solute polyethylene glycol 600 (PEG 600) was used to modify matrically solid media. Bacteria incubation was at 30ºC. After 24, 48 and 72 h of incubation samples from liquid media were spread-plate on nutrient agar medium and incubated 24 h to determine number of viable cells. The bacterial cells were harvested by centrifugation and heat treatment carried out in a water bath at 45ºC during 30 min. The viability of cells from different incubation time in liquid media showed statistically significant differences. Cells of B. amyloliquefaciens grown in liquid media amended with glycerol showed better tolerance at low aw and high survival under heat stress. These results could have important implications for optimizing and to improve formulations.
Fil: Sartori, Melina Victoria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nesci, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Etcheverry, Miriam Graciela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
BIOLOGICAL CONTROL
OSMOTIC ADAPTATION
MAIZE
FUSARIUM VERTICILLIOIDES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/186831
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/186831
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Impact of osmotic/matric stress and heat shock on environmental tolerance induction of bacterial biocontrol agents against Fusarium verticillioidesSartori, Melina VictoriaNesci, Andrea VerónicaEtcheverry, Miriam GracielaBIOLOGICAL CONTROLOSMOTIC ADAPTATIONMAIZEFUSARIUM VERTICILLIOIDEShttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Bacillus amyloliquefaciens and Microbacterium oleovorans reduced Fusarium verticillioides count and decreased significantly fumonisins B1 and B2 levels in maize grains. The aims of this study were to determine the effect of water stress tolerance and heat-shock survival in cells of the biocontrol agents B. amyloliquefaciens and M. oleovorans. The aw of solid and liquid media, tryptic soy medium, was modified to 0.99, 0.98, 0.97 and 0.96 by the addition of the ionic solute NaCl and with the non-ionic solutes such as glycerol and glucose. The non-ionic solute polyethylene glycol 600 (PEG 600) was used to modify matrically solid media. Bacteria incubation was at 30ºC. After 24, 48 and 72 h of incubation samples from liquid media were spread-plate on nutrient agar medium and incubated 24 h to determine number of viable cells. The bacterial cells were harvested by centrifugation and heat treatment carried out in a water bath at 45ºC during 30 min. The viability of cells from different incubation time in liquid media showed statistically significant differences. Cells of B. amyloliquefaciens grown in liquid media amended with glycerol showed better tolerance at low aw and high survival under heat stress. These results could have important implications for optimizing and to improve formulations.Fil: Sartori, Melina Victoria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Nesci, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Etcheverry, Miriam Graciela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science2010-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/186831Sartori, Melina Victoria; Nesci, Andrea Verónica; Etcheverry, Miriam Graciela; Impact of osmotic/matric stress and heat shock on environmental tolerance induction of bacterial biocontrol agents against Fusarium verticillioides; Elsevier Science; Research In Microbiology; 161; 8; 7-2010; 681-6860923-2508CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.resmic.2010.06.008info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0923250810001543info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:26:45Zoai:ri.conicet.gov.ar:11336/186831instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:26:45.856CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Impact of osmotic/matric stress and heat shock on environmental tolerance induction of bacterial biocontrol agents against Fusarium verticillioides
title Impact of osmotic/matric stress and heat shock on environmental tolerance induction of bacterial biocontrol agents against Fusarium verticillioides
spellingShingle Impact of osmotic/matric stress and heat shock on environmental tolerance induction of bacterial biocontrol agents against Fusarium verticillioides
Sartori, Melina Victoria
BIOLOGICAL CONTROL
OSMOTIC ADAPTATION
MAIZE
FUSARIUM VERTICILLIOIDES
title_short Impact of osmotic/matric stress and heat shock on environmental tolerance induction of bacterial biocontrol agents against Fusarium verticillioides
title_full Impact of osmotic/matric stress and heat shock on environmental tolerance induction of bacterial biocontrol agents against Fusarium verticillioides
title_fullStr Impact of osmotic/matric stress and heat shock on environmental tolerance induction of bacterial biocontrol agents against Fusarium verticillioides
title_full_unstemmed Impact of osmotic/matric stress and heat shock on environmental tolerance induction of bacterial biocontrol agents against Fusarium verticillioides
title_sort Impact of osmotic/matric stress and heat shock on environmental tolerance induction of bacterial biocontrol agents against Fusarium verticillioides
dc.creator.none.fl_str_mv Sartori, Melina Victoria
Nesci, Andrea Verónica
Etcheverry, Miriam Graciela
author Sartori, Melina Victoria
author_facet Sartori, Melina Victoria
Nesci, Andrea Verónica
Etcheverry, Miriam Graciela
author_role author
author2 Nesci, Andrea Verónica
Etcheverry, Miriam Graciela
author2_role author
author
dc.subject.none.fl_str_mv BIOLOGICAL CONTROL
OSMOTIC ADAPTATION
MAIZE
FUSARIUM VERTICILLIOIDES
topic BIOLOGICAL CONTROL
OSMOTIC ADAPTATION
MAIZE
FUSARIUM VERTICILLIOIDES
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Bacillus amyloliquefaciens and Microbacterium oleovorans reduced Fusarium verticillioides count and decreased significantly fumonisins B1 and B2 levels in maize grains. The aims of this study were to determine the effect of water stress tolerance and heat-shock survival in cells of the biocontrol agents B. amyloliquefaciens and M. oleovorans. The aw of solid and liquid media, tryptic soy medium, was modified to 0.99, 0.98, 0.97 and 0.96 by the addition of the ionic solute NaCl and with the non-ionic solutes such as glycerol and glucose. The non-ionic solute polyethylene glycol 600 (PEG 600) was used to modify matrically solid media. Bacteria incubation was at 30ºC. After 24, 48 and 72 h of incubation samples from liquid media were spread-plate on nutrient agar medium and incubated 24 h to determine number of viable cells. The bacterial cells were harvested by centrifugation and heat treatment carried out in a water bath at 45ºC during 30 min. The viability of cells from different incubation time in liquid media showed statistically significant differences. Cells of B. amyloliquefaciens grown in liquid media amended with glycerol showed better tolerance at low aw and high survival under heat stress. These results could have important implications for optimizing and to improve formulations.
Fil: Sartori, Melina Victoria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nesci, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Etcheverry, Miriam Graciela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Bacillus amyloliquefaciens and Microbacterium oleovorans reduced Fusarium verticillioides count and decreased significantly fumonisins B1 and B2 levels in maize grains. The aims of this study were to determine the effect of water stress tolerance and heat-shock survival in cells of the biocontrol agents B. amyloliquefaciens and M. oleovorans. The aw of solid and liquid media, tryptic soy medium, was modified to 0.99, 0.98, 0.97 and 0.96 by the addition of the ionic solute NaCl and with the non-ionic solutes such as glycerol and glucose. The non-ionic solute polyethylene glycol 600 (PEG 600) was used to modify matrically solid media. Bacteria incubation was at 30ºC. After 24, 48 and 72 h of incubation samples from liquid media were spread-plate on nutrient agar medium and incubated 24 h to determine number of viable cells. The bacterial cells were harvested by centrifugation and heat treatment carried out in a water bath at 45ºC during 30 min. The viability of cells from different incubation time in liquid media showed statistically significant differences. Cells of B. amyloliquefaciens grown in liquid media amended with glycerol showed better tolerance at low aw and high survival under heat stress. These results could have important implications for optimizing and to improve formulations.
publishDate 2010
dc.date.none.fl_str_mv 2010-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/186831
Sartori, Melina Victoria; Nesci, Andrea Verónica; Etcheverry, Miriam Graciela; Impact of osmotic/matric stress and heat shock on environmental tolerance induction of bacterial biocontrol agents against Fusarium verticillioides; Elsevier Science; Research In Microbiology; 161; 8; 7-2010; 681-686
0923-2508
CONICET Digital
CONICET
url http://hdl.handle.net/11336/186831
identifier_str_mv Sartori, Melina Victoria; Nesci, Andrea Verónica; Etcheverry, Miriam Graciela; Impact of osmotic/matric stress and heat shock on environmental tolerance induction of bacterial biocontrol agents against Fusarium verticillioides; Elsevier Science; Research In Microbiology; 161; 8; 7-2010; 681-686
0923-2508
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.resmic.2010.06.008
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0923250810001543
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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