Effects of maize inoculation with Fusarium verticillioides and with two bacterial biocontrol agents on seedlings growth and antioxidative enzymatic activities

Autores
Pereira, Paola Patricia; Ibañez, Sabrina Guadalupe; Agostini, Elizabeth; Etcheverry, Miriam Graciela
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Maize (Zea mays L.) is a staple food for the majority of the world's population and different diseases may affect its emergence, growth and development. Fusarium verticillioides (Sacc.) Nirenberg (Teleomorph: Gibberella moniliformis Wineland) is the most commonly reported fungal species infecting this crop. The present work analyzes the bioprotective role of two bacterial agents, Bacillus amyloliquefaciens and Microbacterium oleovorans, against F. verticillioides in maize. Maize growth parameters, count and identification of Fusarium isolates as well as CAT, SOD, APX and POD activities were evaluated in 10 and 20 days old seedlings in relation to single inoculations and co-inoculations of the fungus and the bacterial agents. Endophytic count of F. verticillioides propagules was reduced after inoculation with B. amyloliquefaciens during both evaluated periods (10 and 20 days) while M. oleovorans presented a variable performance as an F. verticillioides antagonist. Inoculation with F. verticillioides promoted an increase in SOD activity in roots of 10 days old maize seedlings. S/R ratios of these seedlings were also significantly affected by inoculation with the fungus. Results suggest an early response of maize to infection, since such variations in enzyme activities and plant growth parameters were not observed 20 days after treatment. Moreover, no differences were observed in the number of POD isoforms between infected and non-infected plants. The overall results point out that maize infection by F. verticillioides may be attenuated after seed inoculation with B. amyloliquefaciens and this infection may represent a weak stressful factor, eliciting minor changes in the antioxidative response at early stages of plant growth.
Fil: Pereira, Paola Patricia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Ibañez, Sabrina Guadalupe. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Agostini, Elizabeth. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Etcheverry, Miriam Graciela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
RESPUESTA OXIDATIVA
CONTROL BIOLÓGICO
FUSARIUM VERTICILLIOIDES
MICROBACTERIUM OLEOVORANS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/190510
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/190510
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of maize inoculation with Fusarium verticillioides and with two bacterial biocontrol agents on seedlings growth and antioxidative enzymatic activitiesPereira, Paola PatriciaIbañez, Sabrina GuadalupeAgostini, ElizabethEtcheverry, Miriam GracielaRESPUESTA OXIDATIVACONTROL BIOLÓGICOFUSARIUM VERTICILLIOIDESMICROBACTERIUM OLEOVORANShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Maize (Zea mays L.) is a staple food for the majority of the world's population and different diseases may affect its emergence, growth and development. Fusarium verticillioides (Sacc.) Nirenberg (Teleomorph: Gibberella moniliformis Wineland) is the most commonly reported fungal species infecting this crop. The present work analyzes the bioprotective role of two bacterial agents, Bacillus amyloliquefaciens and Microbacterium oleovorans, against F. verticillioides in maize. Maize growth parameters, count and identification of Fusarium isolates as well as CAT, SOD, APX and POD activities were evaluated in 10 and 20 days old seedlings in relation to single inoculations and co-inoculations of the fungus and the bacterial agents. Endophytic count of F. verticillioides propagules was reduced after inoculation with B. amyloliquefaciens during both evaluated periods (10 and 20 days) while M. oleovorans presented a variable performance as an F. verticillioides antagonist. Inoculation with F. verticillioides promoted an increase in SOD activity in roots of 10 days old maize seedlings. S/R ratios of these seedlings were also significantly affected by inoculation with the fungus. Results suggest an early response of maize to infection, since such variations in enzyme activities and plant growth parameters were not observed 20 days after treatment. Moreover, no differences were observed in the number of POD isoforms between infected and non-infected plants. The overall results point out that maize infection by F. verticillioides may be attenuated after seed inoculation with B. amyloliquefaciens and this infection may represent a weak stressful factor, eliciting minor changes in the antioxidative response at early stages of plant growth.Fil: Pereira, Paola Patricia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Ibañez, Sabrina Guadalupe. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Agostini, Elizabeth. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Etcheverry, Miriam Graciela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science2011-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/190510Pereira, Paola Patricia; Ibañez, Sabrina Guadalupe; Agostini, Elizabeth; Etcheverry, Miriam Graciela; Effects of maize inoculation with Fusarium verticillioides and with two bacterial biocontrol agents on seedlings growth and antioxidative enzymatic activities; Elsevier Science; Applied Soil Ecology; 51; 1; 11-2011; 52-590929-1393CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi//10.1016/j.apsoil.2011.08.007info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0929139311001892info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:43:12Zoai:ri.conicet.gov.ar:11336/190510instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:43:12.32CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of maize inoculation with Fusarium verticillioides and with two bacterial biocontrol agents on seedlings growth and antioxidative enzymatic activities
title Effects of maize inoculation with Fusarium verticillioides and with two bacterial biocontrol agents on seedlings growth and antioxidative enzymatic activities
spellingShingle Effects of maize inoculation with Fusarium verticillioides and with two bacterial biocontrol agents on seedlings growth and antioxidative enzymatic activities
Pereira, Paola Patricia
RESPUESTA OXIDATIVA
CONTROL BIOLÓGICO
FUSARIUM VERTICILLIOIDES
MICROBACTERIUM OLEOVORANS
title_short Effects of maize inoculation with Fusarium verticillioides and with two bacterial biocontrol agents on seedlings growth and antioxidative enzymatic activities
title_full Effects of maize inoculation with Fusarium verticillioides and with two bacterial biocontrol agents on seedlings growth and antioxidative enzymatic activities
title_fullStr Effects of maize inoculation with Fusarium verticillioides and with two bacterial biocontrol agents on seedlings growth and antioxidative enzymatic activities
title_full_unstemmed Effects of maize inoculation with Fusarium verticillioides and with two bacterial biocontrol agents on seedlings growth and antioxidative enzymatic activities
title_sort Effects of maize inoculation with Fusarium verticillioides and with two bacterial biocontrol agents on seedlings growth and antioxidative enzymatic activities
dc.creator.none.fl_str_mv Pereira, Paola Patricia
Ibañez, Sabrina Guadalupe
Agostini, Elizabeth
Etcheverry, Miriam Graciela
author Pereira, Paola Patricia
author_facet Pereira, Paola Patricia
Ibañez, Sabrina Guadalupe
Agostini, Elizabeth
Etcheverry, Miriam Graciela
author_role author
author2 Ibañez, Sabrina Guadalupe
Agostini, Elizabeth
Etcheverry, Miriam Graciela
author2_role author
author
author
dc.subject.none.fl_str_mv RESPUESTA OXIDATIVA
CONTROL BIOLÓGICO
FUSARIUM VERTICILLIOIDES
MICROBACTERIUM OLEOVORANS
topic RESPUESTA OXIDATIVA
CONTROL BIOLÓGICO
FUSARIUM VERTICILLIOIDES
MICROBACTERIUM OLEOVORANS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Maize (Zea mays L.) is a staple food for the majority of the world's population and different diseases may affect its emergence, growth and development. Fusarium verticillioides (Sacc.) Nirenberg (Teleomorph: Gibberella moniliformis Wineland) is the most commonly reported fungal species infecting this crop. The present work analyzes the bioprotective role of two bacterial agents, Bacillus amyloliquefaciens and Microbacterium oleovorans, against F. verticillioides in maize. Maize growth parameters, count and identification of Fusarium isolates as well as CAT, SOD, APX and POD activities were evaluated in 10 and 20 days old seedlings in relation to single inoculations and co-inoculations of the fungus and the bacterial agents. Endophytic count of F. verticillioides propagules was reduced after inoculation with B. amyloliquefaciens during both evaluated periods (10 and 20 days) while M. oleovorans presented a variable performance as an F. verticillioides antagonist. Inoculation with F. verticillioides promoted an increase in SOD activity in roots of 10 days old maize seedlings. S/R ratios of these seedlings were also significantly affected by inoculation with the fungus. Results suggest an early response of maize to infection, since such variations in enzyme activities and plant growth parameters were not observed 20 days after treatment. Moreover, no differences were observed in the number of POD isoforms between infected and non-infected plants. The overall results point out that maize infection by F. verticillioides may be attenuated after seed inoculation with B. amyloliquefaciens and this infection may represent a weak stressful factor, eliciting minor changes in the antioxidative response at early stages of plant growth.
Fil: Pereira, Paola Patricia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Ibañez, Sabrina Guadalupe. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Agostini, Elizabeth. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Etcheverry, Miriam Graciela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Maize (Zea mays L.) is a staple food for the majority of the world's population and different diseases may affect its emergence, growth and development. Fusarium verticillioides (Sacc.) Nirenberg (Teleomorph: Gibberella moniliformis Wineland) is the most commonly reported fungal species infecting this crop. The present work analyzes the bioprotective role of two bacterial agents, Bacillus amyloliquefaciens and Microbacterium oleovorans, against F. verticillioides in maize. Maize growth parameters, count and identification of Fusarium isolates as well as CAT, SOD, APX and POD activities were evaluated in 10 and 20 days old seedlings in relation to single inoculations and co-inoculations of the fungus and the bacterial agents. Endophytic count of F. verticillioides propagules was reduced after inoculation with B. amyloliquefaciens during both evaluated periods (10 and 20 days) while M. oleovorans presented a variable performance as an F. verticillioides antagonist. Inoculation with F. verticillioides promoted an increase in SOD activity in roots of 10 days old maize seedlings. S/R ratios of these seedlings were also significantly affected by inoculation with the fungus. Results suggest an early response of maize to infection, since such variations in enzyme activities and plant growth parameters were not observed 20 days after treatment. Moreover, no differences were observed in the number of POD isoforms between infected and non-infected plants. The overall results point out that maize infection by F. verticillioides may be attenuated after seed inoculation with B. amyloliquefaciens and this infection may represent a weak stressful factor, eliciting minor changes in the antioxidative response at early stages of plant growth.
publishDate 2011
dc.date.none.fl_str_mv 2011-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/190510
Pereira, Paola Patricia; Ibañez, Sabrina Guadalupe; Agostini, Elizabeth; Etcheverry, Miriam Graciela; Effects of maize inoculation with Fusarium verticillioides and with two bacterial biocontrol agents on seedlings growth and antioxidative enzymatic activities; Elsevier Science; Applied Soil Ecology; 51; 1; 11-2011; 52-59
0929-1393
CONICET Digital
CONICET
url http://hdl.handle.net/11336/190510
identifier_str_mv Pereira, Paola Patricia; Ibañez, Sabrina Guadalupe; Agostini, Elizabeth; Etcheverry, Miriam Graciela; Effects of maize inoculation with Fusarium verticillioides and with two bacterial biocontrol agents on seedlings growth and antioxidative enzymatic activities; Elsevier Science; Applied Soil Ecology; 51; 1; 11-2011; 52-59
0929-1393
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi//10.1016/j.apsoil.2011.08.007
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0929139311001892
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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