Protective effect of immunosuppressive treatment before orthotopic kidney autotransplantation

Autores
Cicora, Federico; Lausada, Natalia Raquel; Vasquez, Daniela Noris; Cicora, Paola; Guerrieri, Diego; Gonzalez, Pedro; Zalazar, Gustavo; Stringa, Pablo Luis; Raimondi, Jorge Clemente
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Ischemia reperfusion injury (IRI) is one of the risk factors for delayed graft function, acute rejection and long term allograft survival after kidney transplantation. IRI is an independent antigen inflammatory process that produces tissue damage. Our objective was to study the impact of immunosuppressive treatment (IS) on IRI applying only one dose of IS before orthotopic kidney autotransplantation. Methods: Twenty-four rats allocated in four groups were studied. One group served as control (G1: autotransplanted rats without IS) and the rest received IS 12. h before kidney autotransplantation (G2: Rapamycin, G3: Mycophenolate mofetil and G4: Tacrolimus). Results: Improved renal function and systemic inflammatory response were found among IS groups compared to the control group (Delta Urea p < 0.0001; Delta Creatinine p < 0.0001; Delta C3 p < 0.001). The number of apoptotic nuclei in renal medulla in G1 was higher than in IS groups (p < 0.0001). Tubular damage was less severe in IS groups respecting G1 (p < 0.001). C3, TNF-γ and IL-6 expression in kidney samples was reduced when IS was used compared to the control group. No differences were observed among the different immunosuppressive drugs tested. However, Heme oxygenase-1(HO-1) was increased only in Rapamycin treatment. Conclusions: These data suggest that the use of IS administered before transplant attenuates the IRI process after kidney transplantation in an animal model.
Fil: Cicora, Federico. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina. Hospital Alemán; Argentina. Fundación Para la Investigación y Asistencia de la Enfermedad Renal; Argentina
Fil: Lausada, Natalia Raquel. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
Fil: Vasquez, Daniela Noris. Fundación Para la Investigación y Asistencia de la Enfermedad Renal; Argentina
Fil: Cicora, Paola. Fundación Para la Investigación y Asistencia de la Enfermedad Renal; Argentina
Fil: Guerrieri, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Farmacología; Argentina
Fil: Gonzalez, Pedro. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
Fil: Zalazar, Gustavo. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Stringa, Pablo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
Fil: Raimondi, Jorge Clemente. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
Materia
APOPTOSIS
IMMUNOSUPPRESSION
INFLAMMATORY RESPONSE
ISCHEMIA REPERFUSION INJURY
KIDNEY TRANSPLANT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/190763
Acceder
id CONICETDig_3dcdb0699397838e777d6638989b35f4
oai_identifier_str oai:ri.conicet.gov.ar:11336/190763
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Protective effect of immunosuppressive treatment before orthotopic kidney autotransplantationCicora, FedericoLausada, Natalia RaquelVasquez, Daniela NorisCicora, PaolaGuerrieri, DiegoGonzalez, PedroZalazar, GustavoStringa, Pablo LuisRaimondi, Jorge ClementeAPOPTOSISIMMUNOSUPPRESSIONINFLAMMATORY RESPONSEISCHEMIA REPERFUSION INJURYKIDNEY TRANSPLANThttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Background: Ischemia reperfusion injury (IRI) is one of the risk factors for delayed graft function, acute rejection and long term allograft survival after kidney transplantation. IRI is an independent antigen inflammatory process that produces tissue damage. Our objective was to study the impact of immunosuppressive treatment (IS) on IRI applying only one dose of IS before orthotopic kidney autotransplantation. Methods: Twenty-four rats allocated in four groups were studied. One group served as control (G1: autotransplanted rats without IS) and the rest received IS 12. h before kidney autotransplantation (G2: Rapamycin, G3: Mycophenolate mofetil and G4: Tacrolimus). Results: Improved renal function and systemic inflammatory response were found among IS groups compared to the control group (Delta Urea p < 0.0001; Delta Creatinine p < 0.0001; Delta C3 p < 0.001). The number of apoptotic nuclei in renal medulla in G1 was higher than in IS groups (p < 0.0001). Tubular damage was less severe in IS groups respecting G1 (p < 0.001). C3, TNF-γ and IL-6 expression in kidney samples was reduced when IS was used compared to the control group. No differences were observed among the different immunosuppressive drugs tested. However, Heme oxygenase-1(HO-1) was increased only in Rapamycin treatment. Conclusions: These data suggest that the use of IS administered before transplant attenuates the IRI process after kidney transplantation in an animal model.Fil: Cicora, Federico. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina. Hospital Alemán; Argentina. Fundación Para la Investigación y Asistencia de la Enfermedad Renal; ArgentinaFil: Lausada, Natalia Raquel. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; ArgentinaFil: Vasquez, Daniela Noris. Fundación Para la Investigación y Asistencia de la Enfermedad Renal; ArgentinaFil: Cicora, Paola. Fundación Para la Investigación y Asistencia de la Enfermedad Renal; ArgentinaFil: Guerrieri, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Farmacología; ArgentinaFil: Gonzalez, Pedro. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; ArgentinaFil: Zalazar, Gustavo. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Stringa, Pablo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; ArgentinaFil: Raimondi, Jorge Clemente. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; ArgentinaElsevier Science2011-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/190763Cicora, Federico; Lausada, Natalia Raquel; Vasquez, Daniela Noris; Cicora, Paola; Guerrieri, Diego; et al.; Protective effect of immunosuppressive treatment before orthotopic kidney autotransplantation; Elsevier Science; Transplant Immunology; 24; 2; 1-2011; 107-1120966-3274CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S096632741000119Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.trim.2010.10.006info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T11:25:05Zoai:ri.conicet.gov.ar:11336/190763instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 11:25:05.362CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Protective effect of immunosuppressive treatment before orthotopic kidney autotransplantation
title Protective effect of immunosuppressive treatment before orthotopic kidney autotransplantation
spellingShingle Protective effect of immunosuppressive treatment before orthotopic kidney autotransplantation
Cicora, Federico
APOPTOSIS
IMMUNOSUPPRESSION
INFLAMMATORY RESPONSE
ISCHEMIA REPERFUSION INJURY
KIDNEY TRANSPLANT
title_short Protective effect of immunosuppressive treatment before orthotopic kidney autotransplantation
title_full Protective effect of immunosuppressive treatment before orthotopic kidney autotransplantation
title_fullStr Protective effect of immunosuppressive treatment before orthotopic kidney autotransplantation
title_full_unstemmed Protective effect of immunosuppressive treatment before orthotopic kidney autotransplantation
title_sort Protective effect of immunosuppressive treatment before orthotopic kidney autotransplantation
dc.creator.none.fl_str_mv Cicora, Federico
Lausada, Natalia Raquel
Vasquez, Daniela Noris
Cicora, Paola
Guerrieri, Diego
Gonzalez, Pedro
Zalazar, Gustavo
Stringa, Pablo Luis
Raimondi, Jorge Clemente
author Cicora, Federico
author_facet Cicora, Federico
Lausada, Natalia Raquel
Vasquez, Daniela Noris
Cicora, Paola
Guerrieri, Diego
Gonzalez, Pedro
Zalazar, Gustavo
Stringa, Pablo Luis
Raimondi, Jorge Clemente
author_role author
author2 Lausada, Natalia Raquel
Vasquez, Daniela Noris
Cicora, Paola
Guerrieri, Diego
Gonzalez, Pedro
Zalazar, Gustavo
Stringa, Pablo Luis
Raimondi, Jorge Clemente
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv APOPTOSIS
IMMUNOSUPPRESSION
INFLAMMATORY RESPONSE
ISCHEMIA REPERFUSION INJURY
KIDNEY TRANSPLANT
topic APOPTOSIS
IMMUNOSUPPRESSION
INFLAMMATORY RESPONSE
ISCHEMIA REPERFUSION INJURY
KIDNEY TRANSPLANT
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: Ischemia reperfusion injury (IRI) is one of the risk factors for delayed graft function, acute rejection and long term allograft survival after kidney transplantation. IRI is an independent antigen inflammatory process that produces tissue damage. Our objective was to study the impact of immunosuppressive treatment (IS) on IRI applying only one dose of IS before orthotopic kidney autotransplantation. Methods: Twenty-four rats allocated in four groups were studied. One group served as control (G1: autotransplanted rats without IS) and the rest received IS 12. h before kidney autotransplantation (G2: Rapamycin, G3: Mycophenolate mofetil and G4: Tacrolimus). Results: Improved renal function and systemic inflammatory response were found among IS groups compared to the control group (Delta Urea p < 0.0001; Delta Creatinine p < 0.0001; Delta C3 p < 0.001). The number of apoptotic nuclei in renal medulla in G1 was higher than in IS groups (p < 0.0001). Tubular damage was less severe in IS groups respecting G1 (p < 0.001). C3, TNF-γ and IL-6 expression in kidney samples was reduced when IS was used compared to the control group. No differences were observed among the different immunosuppressive drugs tested. However, Heme oxygenase-1(HO-1) was increased only in Rapamycin treatment. Conclusions: These data suggest that the use of IS administered before transplant attenuates the IRI process after kidney transplantation in an animal model.
Fil: Cicora, Federico. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina. Hospital Alemán; Argentina. Fundación Para la Investigación y Asistencia de la Enfermedad Renal; Argentina
Fil: Lausada, Natalia Raquel. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
Fil: Vasquez, Daniela Noris. Fundación Para la Investigación y Asistencia de la Enfermedad Renal; Argentina
Fil: Cicora, Paola. Fundación Para la Investigación y Asistencia de la Enfermedad Renal; Argentina
Fil: Guerrieri, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Farmacología; Argentina
Fil: Gonzalez, Pedro. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
Fil: Zalazar, Gustavo. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Stringa, Pablo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
Fil: Raimondi, Jorge Clemente. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
description Background: Ischemia reperfusion injury (IRI) is one of the risk factors for delayed graft function, acute rejection and long term allograft survival after kidney transplantation. IRI is an independent antigen inflammatory process that produces tissue damage. Our objective was to study the impact of immunosuppressive treatment (IS) on IRI applying only one dose of IS before orthotopic kidney autotransplantation. Methods: Twenty-four rats allocated in four groups were studied. One group served as control (G1: autotransplanted rats without IS) and the rest received IS 12. h before kidney autotransplantation (G2: Rapamycin, G3: Mycophenolate mofetil and G4: Tacrolimus). Results: Improved renal function and systemic inflammatory response were found among IS groups compared to the control group (Delta Urea p < 0.0001; Delta Creatinine p < 0.0001; Delta C3 p < 0.001). The number of apoptotic nuclei in renal medulla in G1 was higher than in IS groups (p < 0.0001). Tubular damage was less severe in IS groups respecting G1 (p < 0.001). C3, TNF-γ and IL-6 expression in kidney samples was reduced when IS was used compared to the control group. No differences were observed among the different immunosuppressive drugs tested. However, Heme oxygenase-1(HO-1) was increased only in Rapamycin treatment. Conclusions: These data suggest that the use of IS administered before transplant attenuates the IRI process after kidney transplantation in an animal model.
publishDate 2011
dc.date.none.fl_str_mv 2011-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/190763
Cicora, Federico; Lausada, Natalia Raquel; Vasquez, Daniela Noris; Cicora, Paola; Guerrieri, Diego; et al.; Protective effect of immunosuppressive treatment before orthotopic kidney autotransplantation; Elsevier Science; Transplant Immunology; 24; 2; 1-2011; 107-112
0966-3274
CONICET Digital
CONICET
url http://hdl.handle.net/11336/190763
identifier_str_mv Cicora, Federico; Lausada, Natalia Raquel; Vasquez, Daniela Noris; Cicora, Paola; Guerrieri, Diego; et al.; Protective effect of immunosuppressive treatment before orthotopic kidney autotransplantation; Elsevier Science; Transplant Immunology; 24; 2; 1-2011; 107-112
0966-3274
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S096632741000119X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.trim.2010.10.006
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.000565

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