Route of administration influences the concentration of ivermectin reaching nematode parasites in the gastrointestinal tract of cattle

Autores
Leathwick, Dave M.; Miller, C.M.; Waghorn, Tania S.; Schwendel, Heike; Lifschitz, Adrian Luis
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
An animal trial was conducted to measure the concentrations of ivermectin occurring in abomasal and small intestinal contents and mucosa, and in the target parasites (Ostertagia ostertagi and Cooperia oncophora) following administration by subcutaneous, oral and pour-on routes. Twenty-five steers were infected with ivermectin-resistant isolates of O. ostertagi and C. oncophora and following patency randomly allocated to 3 treatment groups of 7 and 1 untreated control group of four. On day 0, animals in the treatment groups were administered ivermectin via the oral, injectable or pour-on routes. On days 1, 2, 3, 4, 5, 6 and 8, blood samples were collected from all live animals, one animal from each treatment group was euthanised and the abomasum and small intestine recovered. Control animals were euthanised on each of days 4, 5, 6 and 8. Samples of gastrointestinal tract organs, their contents, mucosa and parasites were collected and assayed for ivermectin concentration using HPLC. The highest plasma concentrations occurred following subcutaneous administration. In the gastrointestinal contents the highest levels occurred following oral administration, although one high value occurred following pour-on administration, which was attributed to self-licking by the treated animal. The lowest GI content levels followed subcutaneous injection. Ivermectin concentrations in the gastrointestinal mucosa were highest following subcutaneous injection. Drug levels in the abomasal parasite O. ostertagi were most closely correlated with levels in the abomasal mucosa whereas levels in the intestinal C. oncophora were most closely correlated with those in the intestinal contents. Thus, the maximun levels of drug reached C. oncophora in the small intestine following oral administration. In contrast, the highest levels of ivermectin in O. ostertagi followed subcutaneous injection. Therefore, route of administration is likely to influence the exposure to ivermectin for different parasite species.
Fil: Leathwick, Dave M.. Agresearch Grasslands Research Centre; Nueva Zelanda
Fil: Miller, C.M.. Agresearch Grasslands Research Centre; Nueva Zelanda
Fil: Waghorn, Tania S.. Agresearch Grasslands Research Centre; Nueva Zelanda
Fil: Schwendel, Heike. Agresearch Grasslands Research Centre; Nueva Zelanda
Fil: Lifschitz, Adrian Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Materia
CATTLE
COOPERIA
IVERMECTIN
OSTERTAGIA
PHARMACOLOGY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/128517
Acceder
id CONICETDig_3d5cb2aad365de2451769bc4720a658d
oai_identifier_str oai:ri.conicet.gov.ar:11336/128517
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Route of administration influences the concentration of ivermectin reaching nematode parasites in the gastrointestinal tract of cattleLeathwick, Dave M.Miller, C.M.Waghorn, Tania S.Schwendel, HeikeLifschitz, Adrian LuisCATTLECOOPERIAIVERMECTINOSTERTAGIAPHARMACOLOGYhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4An animal trial was conducted to measure the concentrations of ivermectin occurring in abomasal and small intestinal contents and mucosa, and in the target parasites (Ostertagia ostertagi and Cooperia oncophora) following administration by subcutaneous, oral and pour-on routes. Twenty-five steers were infected with ivermectin-resistant isolates of O. ostertagi and C. oncophora and following patency randomly allocated to 3 treatment groups of 7 and 1 untreated control group of four. On day 0, animals in the treatment groups were administered ivermectin via the oral, injectable or pour-on routes. On days 1, 2, 3, 4, 5, 6 and 8, blood samples were collected from all live animals, one animal from each treatment group was euthanised and the abomasum and small intestine recovered. Control animals were euthanised on each of days 4, 5, 6 and 8. Samples of gastrointestinal tract organs, their contents, mucosa and parasites were collected and assayed for ivermectin concentration using HPLC. The highest plasma concentrations occurred following subcutaneous administration. In the gastrointestinal contents the highest levels occurred following oral administration, although one high value occurred following pour-on administration, which was attributed to self-licking by the treated animal. The lowest GI content levels followed subcutaneous injection. Ivermectin concentrations in the gastrointestinal mucosa were highest following subcutaneous injection. Drug levels in the abomasal parasite O. ostertagi were most closely correlated with levels in the abomasal mucosa whereas levels in the intestinal C. oncophora were most closely correlated with those in the intestinal contents. Thus, the maximun levels of drug reached C. oncophora in the small intestine following oral administration. In contrast, the highest levels of ivermectin in O. ostertagi followed subcutaneous injection. Therefore, route of administration is likely to influence the exposure to ivermectin for different parasite species.Fil: Leathwick, Dave M.. Agresearch Grasslands Research Centre; Nueva ZelandaFil: Miller, C.M.. Agresearch Grasslands Research Centre; Nueva ZelandaFil: Waghorn, Tania S.. Agresearch Grasslands Research Centre; Nueva ZelandaFil: Schwendel, Heike. Agresearch Grasslands Research Centre; Nueva ZelandaFil: Lifschitz, Adrian Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaElsevier2020-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/128517Leathwick, Dave M.; Miller, C.M.; Waghorn, Tania S.; Schwendel, Heike; Lifschitz, Adrian Luis; Route of administration influences the concentration of ivermectin reaching nematode parasites in the gastrointestinal tract of cattle; Elsevier; International Journal for Parasitology: Drugs and Drug Resistance; 14; 12-2020; 152-1582211-3207CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijpddr.2020.10.006info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2211320720300385#!info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:27:23Zoai:ri.conicet.gov.ar:11336/128517instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:27:23.871CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Route of administration influences the concentration of ivermectin reaching nematode parasites in the gastrointestinal tract of cattle
title Route of administration influences the concentration of ivermectin reaching nematode parasites in the gastrointestinal tract of cattle
spellingShingle Route of administration influences the concentration of ivermectin reaching nematode parasites in the gastrointestinal tract of cattle
Leathwick, Dave M.
CATTLE
COOPERIA
IVERMECTIN
OSTERTAGIA
PHARMACOLOGY
title_short Route of administration influences the concentration of ivermectin reaching nematode parasites in the gastrointestinal tract of cattle
title_full Route of administration influences the concentration of ivermectin reaching nematode parasites in the gastrointestinal tract of cattle
title_fullStr Route of administration influences the concentration of ivermectin reaching nematode parasites in the gastrointestinal tract of cattle
title_full_unstemmed Route of administration influences the concentration of ivermectin reaching nematode parasites in the gastrointestinal tract of cattle
title_sort Route of administration influences the concentration of ivermectin reaching nematode parasites in the gastrointestinal tract of cattle
dc.creator.none.fl_str_mv Leathwick, Dave M.
Miller, C.M.
Waghorn, Tania S.
Schwendel, Heike
Lifschitz, Adrian Luis
author Leathwick, Dave M.
author_facet Leathwick, Dave M.
Miller, C.M.
Waghorn, Tania S.
Schwendel, Heike
Lifschitz, Adrian Luis
author_role author
author2 Miller, C.M.
Waghorn, Tania S.
Schwendel, Heike
Lifschitz, Adrian Luis
author2_role author
author
author
author
dc.subject.none.fl_str_mv CATTLE
COOPERIA
IVERMECTIN
OSTERTAGIA
PHARMACOLOGY
topic CATTLE
COOPERIA
IVERMECTIN
OSTERTAGIA
PHARMACOLOGY
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv An animal trial was conducted to measure the concentrations of ivermectin occurring in abomasal and small intestinal contents and mucosa, and in the target parasites (Ostertagia ostertagi and Cooperia oncophora) following administration by subcutaneous, oral and pour-on routes. Twenty-five steers were infected with ivermectin-resistant isolates of O. ostertagi and C. oncophora and following patency randomly allocated to 3 treatment groups of 7 and 1 untreated control group of four. On day 0, animals in the treatment groups were administered ivermectin via the oral, injectable or pour-on routes. On days 1, 2, 3, 4, 5, 6 and 8, blood samples were collected from all live animals, one animal from each treatment group was euthanised and the abomasum and small intestine recovered. Control animals were euthanised on each of days 4, 5, 6 and 8. Samples of gastrointestinal tract organs, their contents, mucosa and parasites were collected and assayed for ivermectin concentration using HPLC. The highest plasma concentrations occurred following subcutaneous administration. In the gastrointestinal contents the highest levels occurred following oral administration, although one high value occurred following pour-on administration, which was attributed to self-licking by the treated animal. The lowest GI content levels followed subcutaneous injection. Ivermectin concentrations in the gastrointestinal mucosa were highest following subcutaneous injection. Drug levels in the abomasal parasite O. ostertagi were most closely correlated with levels in the abomasal mucosa whereas levels in the intestinal C. oncophora were most closely correlated with those in the intestinal contents. Thus, the maximun levels of drug reached C. oncophora in the small intestine following oral administration. In contrast, the highest levels of ivermectin in O. ostertagi followed subcutaneous injection. Therefore, route of administration is likely to influence the exposure to ivermectin for different parasite species.
Fil: Leathwick, Dave M.. Agresearch Grasslands Research Centre; Nueva Zelanda
Fil: Miller, C.M.. Agresearch Grasslands Research Centre; Nueva Zelanda
Fil: Waghorn, Tania S.. Agresearch Grasslands Research Centre; Nueva Zelanda
Fil: Schwendel, Heike. Agresearch Grasslands Research Centre; Nueva Zelanda
Fil: Lifschitz, Adrian Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
description An animal trial was conducted to measure the concentrations of ivermectin occurring in abomasal and small intestinal contents and mucosa, and in the target parasites (Ostertagia ostertagi and Cooperia oncophora) following administration by subcutaneous, oral and pour-on routes. Twenty-five steers were infected with ivermectin-resistant isolates of O. ostertagi and C. oncophora and following patency randomly allocated to 3 treatment groups of 7 and 1 untreated control group of four. On day 0, animals in the treatment groups were administered ivermectin via the oral, injectable or pour-on routes. On days 1, 2, 3, 4, 5, 6 and 8, blood samples were collected from all live animals, one animal from each treatment group was euthanised and the abomasum and small intestine recovered. Control animals were euthanised on each of days 4, 5, 6 and 8. Samples of gastrointestinal tract organs, their contents, mucosa and parasites were collected and assayed for ivermectin concentration using HPLC. The highest plasma concentrations occurred following subcutaneous administration. In the gastrointestinal contents the highest levels occurred following oral administration, although one high value occurred following pour-on administration, which was attributed to self-licking by the treated animal. The lowest GI content levels followed subcutaneous injection. Ivermectin concentrations in the gastrointestinal mucosa were highest following subcutaneous injection. Drug levels in the abomasal parasite O. ostertagi were most closely correlated with levels in the abomasal mucosa whereas levels in the intestinal C. oncophora were most closely correlated with those in the intestinal contents. Thus, the maximun levels of drug reached C. oncophora in the small intestine following oral administration. In contrast, the highest levels of ivermectin in O. ostertagi followed subcutaneous injection. Therefore, route of administration is likely to influence the exposure to ivermectin for different parasite species.
publishDate 2020
dc.date.none.fl_str_mv 2020-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/128517
Leathwick, Dave M.; Miller, C.M.; Waghorn, Tania S.; Schwendel, Heike; Lifschitz, Adrian Luis; Route of administration influences the concentration of ivermectin reaching nematode parasites in the gastrointestinal tract of cattle; Elsevier; International Journal for Parasitology: Drugs and Drug Resistance; 14; 12-2020; 152-158
2211-3207
CONICET Digital
CONICET
url http://hdl.handle.net/11336/128517
identifier_str_mv Leathwick, Dave M.; Miller, C.M.; Waghorn, Tania S.; Schwendel, Heike; Lifschitz, Adrian Luis; Route of administration influences the concentration of ivermectin reaching nematode parasites in the gastrointestinal tract of cattle; Elsevier; International Journal for Parasitology: Drugs and Drug Resistance; 14; 12-2020; 152-158
2211-3207
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijpddr.2020.10.006
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2211320720300385#!
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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