Effects of the emulsifiable herbicide Dicamba on amphibian tadpoles: an underestimated toxicity risk?

Autores
Attademo, Andres Maximiliano; Lajmanovich, Rafael Carlos; Peltzer, Paola; Cuzziol Boccioni, Ana Paula; Martinuzzi, Candela Soledad; Simonielo, Fernanda; Repetti, María Rosa
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The effects of exposure to the herbicide Dicamba (DIC) on tadpoles of two amphibian species, Scinax nasicus and Elachistocleis bicolor, were assessed. Mortality and biochemical sublethal effects were evaluated using acetylcholinesterase (AChE), glutathione S-transferase (GST), glutathione reductase (GR), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) activities and thyroid hormone (T4) levels. The LC50 value at 48h was 0.859 mg L−1 for S. nasicus and 0.221 mg L−1 for E. bicolor tadpoles. After exposure to sublethal DIC concentrations for 48 h, GST activity increased in S. nasicus but significantly decreased in E. bicolor with respect to controls. GR activity decreased only in S. nasicus at all the tested DIC concentrations. AChE activity was significantly inhibited in both S. nasicus and E. bicolor tadpoles at 48 h. DIC also caused significant changes in transamination, as evidenced by an increase in AST and ALT activities in both amphibian species. T4 levels were higher in DIC-treated tadpoles of both species than in controls. The DIC-induced biochemical alterations in glutathione system enzymes and transaminases indicate lesions in liver tissues and cellular function. Moreover, the observed AChE inhibition could lead to the accumulation of acetylcholine, excessively stimulating postsynaptic receptors, and the increase in T4 levels in both species may indicate an overactive thyroid. The commercial DIC formulation showed a high biotoxicity in the two amphibian native species after short-term exposure, controversially differing from the toxicity level indicated in the official fact sheet data. This fact highlights the need for an urgent re-categorization and reevaluation of DIC toxicity in native species.
Fil: Attademo, Andres Maximiliano. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Lajmanovich, Rafael Carlos. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Peltzer, Paola. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Cuzziol Boccioni, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Martinuzzi, Candela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Simonielo, Fernanda. Universidad Nacional del Litoral; Argentina
Fil: Repetti, María Rosa. Universidad Nacional del Litoral; Argentina
Materia
ACETYLCHOLINESTERASE
ANTIOXIDANT SYSTEMS
BIOMARKER
DICAMBA
HERBICIDE
TADPOLES
THYROID HORMONE
TRANSAMINASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/165105
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/165105
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of the emulsifiable herbicide Dicamba on amphibian tadpoles: an underestimated toxicity risk?Attademo, Andres MaximilianoLajmanovich, Rafael CarlosPeltzer, PaolaCuzziol Boccioni, Ana PaulaMartinuzzi, Candela SoledadSimonielo, FernandaRepetti, María RosaACETYLCHOLINESTERASEANTIOXIDANT SYSTEMSBIOMARKERDICAMBAHERBICIDETADPOLESTHYROID HORMONETRANSAMINASEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The effects of exposure to the herbicide Dicamba (DIC) on tadpoles of two amphibian species, Scinax nasicus and Elachistocleis bicolor, were assessed. Mortality and biochemical sublethal effects were evaluated using acetylcholinesterase (AChE), glutathione S-transferase (GST), glutathione reductase (GR), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) activities and thyroid hormone (T4) levels. The LC50 value at 48h was 0.859 mg L−1 for S. nasicus and 0.221 mg L−1 for E. bicolor tadpoles. After exposure to sublethal DIC concentrations for 48 h, GST activity increased in S. nasicus but significantly decreased in E. bicolor with respect to controls. GR activity decreased only in S. nasicus at all the tested DIC concentrations. AChE activity was significantly inhibited in both S. nasicus and E. bicolor tadpoles at 48 h. DIC also caused significant changes in transamination, as evidenced by an increase in AST and ALT activities in both amphibian species. T4 levels were higher in DIC-treated tadpoles of both species than in controls. The DIC-induced biochemical alterations in glutathione system enzymes and transaminases indicate lesions in liver tissues and cellular function. Moreover, the observed AChE inhibition could lead to the accumulation of acetylcholine, excessively stimulating postsynaptic receptors, and the increase in T4 levels in both species may indicate an overactive thyroid. The commercial DIC formulation showed a high biotoxicity in the two amphibian native species after short-term exposure, controversially differing from the toxicity level indicated in the official fact sheet data. This fact highlights the need for an urgent re-categorization and reevaluation of DIC toxicity in native species.Fil: Attademo, Andres Maximiliano. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Lajmanovich, Rafael Carlos. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Peltzer, Paola. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Cuzziol Boccioni, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Martinuzzi, Candela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Simonielo, Fernanda. Universidad Nacional del Litoral; ArgentinaFil: Repetti, María Rosa. Universidad Nacional del Litoral; ArgentinaSpringer Heidelberg2021-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/165105Attademo, Andres Maximiliano; Lajmanovich, Rafael Carlos; Peltzer, Paola; Cuzziol Boccioni, Ana Paula; Martinuzzi, Candela Soledad; et al.; Effects of the emulsifiable herbicide Dicamba on amphibian tadpoles: an underestimated toxicity risk?; Springer Heidelberg; Environmental Science and Pollution Research; 28; 24; 2-2021; 31962-319740944-1344CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s11356-021-13000-xinfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11356-021-13000-xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:08:19Zoai:ri.conicet.gov.ar:11336/165105instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:08:19.352CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of the emulsifiable herbicide Dicamba on amphibian tadpoles: an underestimated toxicity risk?
title Effects of the emulsifiable herbicide Dicamba on amphibian tadpoles: an underestimated toxicity risk?
spellingShingle Effects of the emulsifiable herbicide Dicamba on amphibian tadpoles: an underestimated toxicity risk?
Attademo, Andres Maximiliano
ACETYLCHOLINESTERASE
ANTIOXIDANT SYSTEMS
BIOMARKER
DICAMBA
HERBICIDE
TADPOLES
THYROID HORMONE
TRANSAMINASE
title_short Effects of the emulsifiable herbicide Dicamba on amphibian tadpoles: an underestimated toxicity risk?
title_full Effects of the emulsifiable herbicide Dicamba on amphibian tadpoles: an underestimated toxicity risk?
title_fullStr Effects of the emulsifiable herbicide Dicamba on amphibian tadpoles: an underestimated toxicity risk?
title_full_unstemmed Effects of the emulsifiable herbicide Dicamba on amphibian tadpoles: an underestimated toxicity risk?
title_sort Effects of the emulsifiable herbicide Dicamba on amphibian tadpoles: an underestimated toxicity risk?
dc.creator.none.fl_str_mv Attademo, Andres Maximiliano
Lajmanovich, Rafael Carlos
Peltzer, Paola
Cuzziol Boccioni, Ana Paula
Martinuzzi, Candela Soledad
Simonielo, Fernanda
Repetti, María Rosa
author Attademo, Andres Maximiliano
author_facet Attademo, Andres Maximiliano
Lajmanovich, Rafael Carlos
Peltzer, Paola
Cuzziol Boccioni, Ana Paula
Martinuzzi, Candela Soledad
Simonielo, Fernanda
Repetti, María Rosa
author_role author
author2 Lajmanovich, Rafael Carlos
Peltzer, Paola
Cuzziol Boccioni, Ana Paula
Martinuzzi, Candela Soledad
Simonielo, Fernanda
Repetti, María Rosa
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ACETYLCHOLINESTERASE
ANTIOXIDANT SYSTEMS
BIOMARKER
DICAMBA
HERBICIDE
TADPOLES
THYROID HORMONE
TRANSAMINASE
topic ACETYLCHOLINESTERASE
ANTIOXIDANT SYSTEMS
BIOMARKER
DICAMBA
HERBICIDE
TADPOLES
THYROID HORMONE
TRANSAMINASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The effects of exposure to the herbicide Dicamba (DIC) on tadpoles of two amphibian species, Scinax nasicus and Elachistocleis bicolor, were assessed. Mortality and biochemical sublethal effects were evaluated using acetylcholinesterase (AChE), glutathione S-transferase (GST), glutathione reductase (GR), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) activities and thyroid hormone (T4) levels. The LC50 value at 48h was 0.859 mg L−1 for S. nasicus and 0.221 mg L−1 for E. bicolor tadpoles. After exposure to sublethal DIC concentrations for 48 h, GST activity increased in S. nasicus but significantly decreased in E. bicolor with respect to controls. GR activity decreased only in S. nasicus at all the tested DIC concentrations. AChE activity was significantly inhibited in both S. nasicus and E. bicolor tadpoles at 48 h. DIC also caused significant changes in transamination, as evidenced by an increase in AST and ALT activities in both amphibian species. T4 levels were higher in DIC-treated tadpoles of both species than in controls. The DIC-induced biochemical alterations in glutathione system enzymes and transaminases indicate lesions in liver tissues and cellular function. Moreover, the observed AChE inhibition could lead to the accumulation of acetylcholine, excessively stimulating postsynaptic receptors, and the increase in T4 levels in both species may indicate an overactive thyroid. The commercial DIC formulation showed a high biotoxicity in the two amphibian native species after short-term exposure, controversially differing from the toxicity level indicated in the official fact sheet data. This fact highlights the need for an urgent re-categorization and reevaluation of DIC toxicity in native species.
Fil: Attademo, Andres Maximiliano. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Lajmanovich, Rafael Carlos. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Peltzer, Paola. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Cuzziol Boccioni, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Martinuzzi, Candela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Simonielo, Fernanda. Universidad Nacional del Litoral; Argentina
Fil: Repetti, María Rosa. Universidad Nacional del Litoral; Argentina
description The effects of exposure to the herbicide Dicamba (DIC) on tadpoles of two amphibian species, Scinax nasicus and Elachistocleis bicolor, were assessed. Mortality and biochemical sublethal effects were evaluated using acetylcholinesterase (AChE), glutathione S-transferase (GST), glutathione reductase (GR), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) activities and thyroid hormone (T4) levels. The LC50 value at 48h was 0.859 mg L−1 for S. nasicus and 0.221 mg L−1 for E. bicolor tadpoles. After exposure to sublethal DIC concentrations for 48 h, GST activity increased in S. nasicus but significantly decreased in E. bicolor with respect to controls. GR activity decreased only in S. nasicus at all the tested DIC concentrations. AChE activity was significantly inhibited in both S. nasicus and E. bicolor tadpoles at 48 h. DIC also caused significant changes in transamination, as evidenced by an increase in AST and ALT activities in both amphibian species. T4 levels were higher in DIC-treated tadpoles of both species than in controls. The DIC-induced biochemical alterations in glutathione system enzymes and transaminases indicate lesions in liver tissues and cellular function. Moreover, the observed AChE inhibition could lead to the accumulation of acetylcholine, excessively stimulating postsynaptic receptors, and the increase in T4 levels in both species may indicate an overactive thyroid. The commercial DIC formulation showed a high biotoxicity in the two amphibian native species after short-term exposure, controversially differing from the toxicity level indicated in the official fact sheet data. This fact highlights the need for an urgent re-categorization and reevaluation of DIC toxicity in native species.
publishDate 2021
dc.date.none.fl_str_mv 2021-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/165105
Attademo, Andres Maximiliano; Lajmanovich, Rafael Carlos; Peltzer, Paola; Cuzziol Boccioni, Ana Paula; Martinuzzi, Candela Soledad; et al.; Effects of the emulsifiable herbicide Dicamba on amphibian tadpoles: an underestimated toxicity risk?; Springer Heidelberg; Environmental Science and Pollution Research; 28; 24; 2-2021; 31962-31974
0944-1344
CONICET Digital
CONICET
url http://hdl.handle.net/11336/165105
identifier_str_mv Attademo, Andres Maximiliano; Lajmanovich, Rafael Carlos; Peltzer, Paola; Cuzziol Boccioni, Ana Paula; Martinuzzi, Candela Soledad; et al.; Effects of the emulsifiable herbicide Dicamba on amphibian tadpoles: an underestimated toxicity risk?; Springer Heidelberg; Environmental Science and Pollution Research; 28; 24; 2-2021; 31962-31974
0944-1344
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s11356-021-13000-x
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11356-021-13000-x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Heidelberg
publisher.none.fl_str_mv Springer Heidelberg
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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