Acute Toxicity and Sublethal Effects Caused by a Commercial Herbicide Formulated with 2,4-D on Physalaemus albonotatus Tadpoles

Autores
Curi, Lucila Marilén; Peltzer, Paola; Sandoval, María Teresa; Lajmanovich, Rafael Carlos
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to evaluate the acute and chronic effects caused by exposure to the 2,4 dichlorophenoxyacetic acid (2,4-D)-based commercial herbicide Amina Zamba® on Physalaemus albonotatus tadpoles from Gosner stage 25. The lethal concentration (LC50) was determined after exposure to different concentrations of Amina Zamba® (350 to 2400 mg/L) at 96 h. Sublethal effects were evaluated after chronic exposure to four fractions of the LC50 96h obtained (12.5, 25, 50, and 75% of LC50 96h ) and a control. The biological responses analyzed included survival, growth and development, morphological abnormalities, and histological changes in the liver. The LC50 values of Amina Zamba® at 48, 72, and 96 h were 1040.2, 754.2, and 350 mg/L, respectively. The chronic exposure to the herbicide altered the survival of exposed tadpoles and caused several morphological abnormalities and liver histological alterations, mainly at the highest concentrations tested. Oral disc malformations and intestinal abnormalities were the most frequent abnormalities in all treated tadpoles. Histological alterations observed in the liver structure included hepatocyte vacuolization, enlargement of sinusoids, dilation of blood vessels, and a significant increase in the number of melanomacrophages in tadpoles exposed to 25, 50, and 75% LC50 96h with respect to control (P < 0.05). Furthermore, the treated tadpoles showed an accelerated development rate, reaching Gosner stages 38 and 42 before controls. These results demonstrate that the chronic exposure to this commercial formulation affects the survival, accelerates metamorphosis, and induces morphological abnormalities and liver damage in P. albonotatus tadpoles.
Fil: Curi, Lucila Marilén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Laboratorio de Ecotoxicologia; Argentina
Fil: Peltzer, Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Laboratorio de Ecotoxicologia; Argentina
Fil: Sandoval, María Teresa. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas, Naturales y Agrimensura. Departamento de Biología; Argentina
Fil: Lajmanovich, Rafael Carlos. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Laboratorio de Ecotoxicologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Materia
ABNORMALITIES
HERBICIDE
HISTOLOGY
MELANOMACROPHAGES
TADPOLES
TOXICITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/118815
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/118815
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Acute Toxicity and Sublethal Effects Caused by a Commercial Herbicide Formulated with 2,4-D on Physalaemus albonotatus TadpolesCuri, Lucila MarilénPeltzer, PaolaSandoval, María TeresaLajmanovich, Rafael CarlosABNORMALITIESHERBICIDEHISTOLOGYMELANOMACROPHAGESTADPOLESTOXICITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of this study was to evaluate the acute and chronic effects caused by exposure to the 2,4 dichlorophenoxyacetic acid (2,4-D)-based commercial herbicide Amina Zamba® on Physalaemus albonotatus tadpoles from Gosner stage 25. The lethal concentration (LC50) was determined after exposure to different concentrations of Amina Zamba® (350 to 2400 mg/L) at 96 h. Sublethal effects were evaluated after chronic exposure to four fractions of the LC50 96h obtained (12.5, 25, 50, and 75% of LC50 96h ) and a control. The biological responses analyzed included survival, growth and development, morphological abnormalities, and histological changes in the liver. The LC50 values of Amina Zamba® at 48, 72, and 96 h were 1040.2, 754.2, and 350 mg/L, respectively. The chronic exposure to the herbicide altered the survival of exposed tadpoles and caused several morphological abnormalities and liver histological alterations, mainly at the highest concentrations tested. Oral disc malformations and intestinal abnormalities were the most frequent abnormalities in all treated tadpoles. Histological alterations observed in the liver structure included hepatocyte vacuolization, enlargement of sinusoids, dilation of blood vessels, and a significant increase in the number of melanomacrophages in tadpoles exposed to 25, 50, and 75% LC50 96h with respect to control (P < 0.05). Furthermore, the treated tadpoles showed an accelerated development rate, reaching Gosner stages 38 and 42 before controls. These results demonstrate that the chronic exposure to this commercial formulation affects the survival, accelerates metamorphosis, and induces morphological abnormalities and liver damage in P. albonotatus tadpoles.Fil: Curi, Lucila Marilén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Laboratorio de Ecotoxicologia; ArgentinaFil: Peltzer, Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Laboratorio de Ecotoxicologia; ArgentinaFil: Sandoval, María Teresa. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas, Naturales y Agrimensura. Departamento de Biología; ArgentinaFil: Lajmanovich, Rafael Carlos. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Laboratorio de Ecotoxicologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaSpringer2019-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/118815Curi, Lucila Marilén; Peltzer, Paola; Sandoval, María Teresa; Lajmanovich, Rafael Carlos; Acute Toxicity and Sublethal Effects Caused by a Commercial Herbicide Formulated with 2,4-D on Physalaemus albonotatus Tadpoles; Springer; Water, Air and Soil Pollution; 230; 22; 1-2019; 1-220049-69790049-6979CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s11270-018-4073-xinfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11270-018-4073-xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:55:53Zoai:ri.conicet.gov.ar:11336/118815instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:55:54.113CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Acute Toxicity and Sublethal Effects Caused by a Commercial Herbicide Formulated with 2,4-D on Physalaemus albonotatus Tadpoles
title Acute Toxicity and Sublethal Effects Caused by a Commercial Herbicide Formulated with 2,4-D on Physalaemus albonotatus Tadpoles
spellingShingle Acute Toxicity and Sublethal Effects Caused by a Commercial Herbicide Formulated with 2,4-D on Physalaemus albonotatus Tadpoles
Curi, Lucila Marilén
ABNORMALITIES
HERBICIDE
HISTOLOGY
MELANOMACROPHAGES
TADPOLES
TOXICITY
title_short Acute Toxicity and Sublethal Effects Caused by a Commercial Herbicide Formulated with 2,4-D on Physalaemus albonotatus Tadpoles
title_full Acute Toxicity and Sublethal Effects Caused by a Commercial Herbicide Formulated with 2,4-D on Physalaemus albonotatus Tadpoles
title_fullStr Acute Toxicity and Sublethal Effects Caused by a Commercial Herbicide Formulated with 2,4-D on Physalaemus albonotatus Tadpoles
title_full_unstemmed Acute Toxicity and Sublethal Effects Caused by a Commercial Herbicide Formulated with 2,4-D on Physalaemus albonotatus Tadpoles
title_sort Acute Toxicity and Sublethal Effects Caused by a Commercial Herbicide Formulated with 2,4-D on Physalaemus albonotatus Tadpoles
dc.creator.none.fl_str_mv Curi, Lucila Marilén
Peltzer, Paola
Sandoval, María Teresa
Lajmanovich, Rafael Carlos
author Curi, Lucila Marilén
author_facet Curi, Lucila Marilén
Peltzer, Paola
Sandoval, María Teresa
Lajmanovich, Rafael Carlos
author_role author
author2 Peltzer, Paola
Sandoval, María Teresa
Lajmanovich, Rafael Carlos
author2_role author
author
author
dc.subject.none.fl_str_mv ABNORMALITIES
HERBICIDE
HISTOLOGY
MELANOMACROPHAGES
TADPOLES
TOXICITY
topic ABNORMALITIES
HERBICIDE
HISTOLOGY
MELANOMACROPHAGES
TADPOLES
TOXICITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of this study was to evaluate the acute and chronic effects caused by exposure to the 2,4 dichlorophenoxyacetic acid (2,4-D)-based commercial herbicide Amina Zamba® on Physalaemus albonotatus tadpoles from Gosner stage 25. The lethal concentration (LC50) was determined after exposure to different concentrations of Amina Zamba® (350 to 2400 mg/L) at 96 h. Sublethal effects were evaluated after chronic exposure to four fractions of the LC50 96h obtained (12.5, 25, 50, and 75% of LC50 96h ) and a control. The biological responses analyzed included survival, growth and development, morphological abnormalities, and histological changes in the liver. The LC50 values of Amina Zamba® at 48, 72, and 96 h were 1040.2, 754.2, and 350 mg/L, respectively. The chronic exposure to the herbicide altered the survival of exposed tadpoles and caused several morphological abnormalities and liver histological alterations, mainly at the highest concentrations tested. Oral disc malformations and intestinal abnormalities were the most frequent abnormalities in all treated tadpoles. Histological alterations observed in the liver structure included hepatocyte vacuolization, enlargement of sinusoids, dilation of blood vessels, and a significant increase in the number of melanomacrophages in tadpoles exposed to 25, 50, and 75% LC50 96h with respect to control (P < 0.05). Furthermore, the treated tadpoles showed an accelerated development rate, reaching Gosner stages 38 and 42 before controls. These results demonstrate that the chronic exposure to this commercial formulation affects the survival, accelerates metamorphosis, and induces morphological abnormalities and liver damage in P. albonotatus tadpoles.
Fil: Curi, Lucila Marilén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Laboratorio de Ecotoxicologia; Argentina
Fil: Peltzer, Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Laboratorio de Ecotoxicologia; Argentina
Fil: Sandoval, María Teresa. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas, Naturales y Agrimensura. Departamento de Biología; Argentina
Fil: Lajmanovich, Rafael Carlos. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Laboratorio de Ecotoxicologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
description The aim of this study was to evaluate the acute and chronic effects caused by exposure to the 2,4 dichlorophenoxyacetic acid (2,4-D)-based commercial herbicide Amina Zamba® on Physalaemus albonotatus tadpoles from Gosner stage 25. The lethal concentration (LC50) was determined after exposure to different concentrations of Amina Zamba® (350 to 2400 mg/L) at 96 h. Sublethal effects were evaluated after chronic exposure to four fractions of the LC50 96h obtained (12.5, 25, 50, and 75% of LC50 96h ) and a control. The biological responses analyzed included survival, growth and development, morphological abnormalities, and histological changes in the liver. The LC50 values of Amina Zamba® at 48, 72, and 96 h were 1040.2, 754.2, and 350 mg/L, respectively. The chronic exposure to the herbicide altered the survival of exposed tadpoles and caused several morphological abnormalities and liver histological alterations, mainly at the highest concentrations tested. Oral disc malformations and intestinal abnormalities were the most frequent abnormalities in all treated tadpoles. Histological alterations observed in the liver structure included hepatocyte vacuolization, enlargement of sinusoids, dilation of blood vessels, and a significant increase in the number of melanomacrophages in tadpoles exposed to 25, 50, and 75% LC50 96h with respect to control (P < 0.05). Furthermore, the treated tadpoles showed an accelerated development rate, reaching Gosner stages 38 and 42 before controls. These results demonstrate that the chronic exposure to this commercial formulation affects the survival, accelerates metamorphosis, and induces morphological abnormalities and liver damage in P. albonotatus tadpoles.
publishDate 2019
dc.date.none.fl_str_mv 2019-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/118815
Curi, Lucila Marilén; Peltzer, Paola; Sandoval, María Teresa; Lajmanovich, Rafael Carlos; Acute Toxicity and Sublethal Effects Caused by a Commercial Herbicide Formulated with 2,4-D on Physalaemus albonotatus Tadpoles; Springer; Water, Air and Soil Pollution; 230; 22; 1-2019; 1-22
0049-6979
0049-6979
CONICET Digital
CONICET
url http://hdl.handle.net/11336/118815
identifier_str_mv Curi, Lucila Marilén; Peltzer, Paola; Sandoval, María Teresa; Lajmanovich, Rafael Carlos; Acute Toxicity and Sublethal Effects Caused by a Commercial Herbicide Formulated with 2,4-D on Physalaemus albonotatus Tadpoles; Springer; Water, Air and Soil Pollution; 230; 22; 1-2019; 1-22
0049-6979
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s11270-018-4073-x
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11270-018-4073-x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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