Multilocus Sequence Typing (MLST) for Lineage Assignment and High Resolution Diversity Studies in Trypanosoma cruzi
- Autores
- Yeo, Matthew; Mauricio, Isabel L.; Messenger, Loisa A.; Lewis, Michael D.; Llewellyn, Martin S.; Acosta, Nidia; Bhattacharyya, Tapan; Diosque, Patricio; Carrasco, Hernán J.; Miles, Michael A.
- Año de publicación
- 2011
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- PLoS Negl Trop Dis. 2011 Jun;5(6):e1049. Epub 2011 Jun 21. Multilocus sequence typing (MLST) for lineage assignment and high resolution diversity studies in Trypanosoma cruzi. Yeo M, Mauricio IL, Messenger LA, Lewis MD, Llewellyn MS, Acosta N, Bhattacharyya T, Diosque P, Carrasco HJ, Miles MA. Source London School of Hygiene and Tropical Medicine, London, United Kingdom. Matthew.yeo@lshtm.ac.uk Abstract BACKGROUND: Multilocus sequence typing (MLST) is a powerful and highly discriminatory method for analysing pathogen population structure and epidemiology. Trypanosoma cruzi, the protozoan agent of American trypanosomiasis (Chagas disease), has remarkable genetic and ecological diversity. A standardised MLST protocol that is suitable for assignment of T. cruzi isolates to genetic lineage and for higher resolution diversity studies has not been developed. METHODOLOGY/PRINCIPAL FINDINGS: We have sequenced and diplotyped nine single copy housekeeping genes and assessed their value as part of a systematic MLST scheme for T. cruzi. A minimum panel of four MLST targets (Met-III, RB19, TcGPXII, and DHFR-TS) was shown to provide unambiguous assignment of isolates to the six known T. cruzi lineages (Discrete Typing Units, DTUs TcI-TcVI). In addition, we recommend six MLST targets (Met-II, Met-III, RB19, TcMPX, DHFR-TS, and TR) for more in depth diversity studies on the basis that diploid sequence typing (DST) with this expanded panel distinguished 38 out of 39 reference isolates. Phylogenetic analysis implies a subdivision between North and South American TcIV isolates. Single Nucleotide Polymorphism (SNP) data revealed high levels of heterozygosity among DTUs TcI, TcIII, TcIV and, for three targets, putative corresponding homozygous and heterozygous loci within DTUs TcI and TcIII. Furthermore, individual gene trees gave incongruent topologies at inter- and intra-DTU levels, inconsistent with a model of strict clonality. CONCLUSIONS/SIGNIFICANCE: We demonstrate the value of systematic MLST diplotyping for describing inter-DTU relationships and for higher resolution diversity studies of T. cruzi, including presence of recombination events. The high levels of heterozygosity will facilitate future population genetics analysis based on MLST haplotypes.
Fil: Yeo, Matthew. London School of Hygiene and Tropical Medicine; Reino Unido
Fil: Mauricio, Isabel L.. Instituto de Higiene e Medicina Tropical; Portugal
Fil: Messenger, Loisa A.. London School of Hygiene and Tropical Medicine; Reino Unido
Fil: Lewis, Michael D.. London School of Hygiene and Tropical Medicine; Reino Unido
Fil: Llewellyn, Martin S.. London School of Hygiene and Tropical Medicine; Reino Unido
Fil: Acosta, Nidia. London School of Hygiene and Tropical Medicine; Reino Unido. Universidad Nacional de Asuncion; Paraguay
Fil: Bhattacharyya, Tapan. London School of Hygiene and Tropical Medicine; Reino Unido
Fil: Diosque, Patricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Cs.de la Salud. Instituto de Patología Experimental; Argentina
Fil: Carrasco, Hernán J.. Universidad Central de Venezuela; Venezuela
Fil: Miles, Michael A.. London School of Hygiene and Tropical Medicine; Reino Unido - Materia
-
MLST
TYPING
TRYPANOSOMA CRUZI - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/16813
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Multilocus Sequence Typing (MLST) for Lineage Assignment and High Resolution Diversity Studies in Trypanosoma cruziYeo, MatthewMauricio, Isabel L.Messenger, Loisa A.Lewis, Michael D.Llewellyn, Martin S.Acosta, NidiaBhattacharyya, TapanDiosque, PatricioCarrasco, Hernán J.Miles, Michael A.MLSTTYPINGTRYPANOSOMA CRUZIhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3PLoS Negl Trop Dis. 2011 Jun;5(6):e1049. Epub 2011 Jun 21. Multilocus sequence typing (MLST) for lineage assignment and high resolution diversity studies in Trypanosoma cruzi. Yeo M, Mauricio IL, Messenger LA, Lewis MD, Llewellyn MS, Acosta N, Bhattacharyya T, Diosque P, Carrasco HJ, Miles MA. Source London School of Hygiene and Tropical Medicine, London, United Kingdom. Matthew.yeo@lshtm.ac.uk Abstract BACKGROUND: Multilocus sequence typing (MLST) is a powerful and highly discriminatory method for analysing pathogen population structure and epidemiology. Trypanosoma cruzi, the protozoan agent of American trypanosomiasis (Chagas disease), has remarkable genetic and ecological diversity. A standardised MLST protocol that is suitable for assignment of T. cruzi isolates to genetic lineage and for higher resolution diversity studies has not been developed. METHODOLOGY/PRINCIPAL FINDINGS: We have sequenced and diplotyped nine single copy housekeeping genes and assessed their value as part of a systematic MLST scheme for T. cruzi. A minimum panel of four MLST targets (Met-III, RB19, TcGPXII, and DHFR-TS) was shown to provide unambiguous assignment of isolates to the six known T. cruzi lineages (Discrete Typing Units, DTUs TcI-TcVI). In addition, we recommend six MLST targets (Met-II, Met-III, RB19, TcMPX, DHFR-TS, and TR) for more in depth diversity studies on the basis that diploid sequence typing (DST) with this expanded panel distinguished 38 out of 39 reference isolates. Phylogenetic analysis implies a subdivision between North and South American TcIV isolates. Single Nucleotide Polymorphism (SNP) data revealed high levels of heterozygosity among DTUs TcI, TcIII, TcIV and, for three targets, putative corresponding homozygous and heterozygous loci within DTUs TcI and TcIII. Furthermore, individual gene trees gave incongruent topologies at inter- and intra-DTU levels, inconsistent with a model of strict clonality. CONCLUSIONS/SIGNIFICANCE: We demonstrate the value of systematic MLST diplotyping for describing inter-DTU relationships and for higher resolution diversity studies of T. cruzi, including presence of recombination events. The high levels of heterozygosity will facilitate future population genetics analysis based on MLST haplotypes.Fil: Yeo, Matthew. London School of Hygiene and Tropical Medicine; Reino UnidoFil: Mauricio, Isabel L.. Instituto de Higiene e Medicina Tropical; PortugalFil: Messenger, Loisa A.. London School of Hygiene and Tropical Medicine; Reino UnidoFil: Lewis, Michael D.. London School of Hygiene and Tropical Medicine; Reino UnidoFil: Llewellyn, Martin S.. London School of Hygiene and Tropical Medicine; Reino UnidoFil: Acosta, Nidia. London School of Hygiene and Tropical Medicine; Reino Unido. Universidad Nacional de Asuncion; ParaguayFil: Bhattacharyya, Tapan. London School of Hygiene and Tropical Medicine; Reino UnidoFil: Diosque, Patricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Cs.de la Salud. Instituto de Patología Experimental; ArgentinaFil: Carrasco, Hernán J.. Universidad Central de Venezuela; VenezuelaFil: Miles, Michael A.. London School of Hygiene and Tropical Medicine; Reino UnidoPublic Library Of Science2011-06-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16813Yeo, Matthew; Mauricio, Isabel L.; Messenger, Loisa A.; Lewis, Michael D.; Llewellyn, Martin S.; et al.; Multilocus Sequence Typing (MLST) for Lineage Assignment and High Resolution Diversity Studies in Trypanosoma cruzi; Public Library Of Science; Neglected Tropical Diseases; 5; 6; 21-6-2011; 1049-10491935-2735enginfo:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0001049info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0001049info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:27:49Zoai:ri.conicet.gov.ar:11336/16813instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:27:50.165CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Multilocus Sequence Typing (MLST) for Lineage Assignment and High Resolution Diversity Studies in Trypanosoma cruzi |
title |
Multilocus Sequence Typing (MLST) for Lineage Assignment and High Resolution Diversity Studies in Trypanosoma cruzi |
spellingShingle |
Multilocus Sequence Typing (MLST) for Lineage Assignment and High Resolution Diversity Studies in Trypanosoma cruzi Yeo, Matthew MLST TYPING TRYPANOSOMA CRUZI |
title_short |
Multilocus Sequence Typing (MLST) for Lineage Assignment and High Resolution Diversity Studies in Trypanosoma cruzi |
title_full |
Multilocus Sequence Typing (MLST) for Lineage Assignment and High Resolution Diversity Studies in Trypanosoma cruzi |
title_fullStr |
Multilocus Sequence Typing (MLST) for Lineage Assignment and High Resolution Diversity Studies in Trypanosoma cruzi |
title_full_unstemmed |
Multilocus Sequence Typing (MLST) for Lineage Assignment and High Resolution Diversity Studies in Trypanosoma cruzi |
title_sort |
Multilocus Sequence Typing (MLST) for Lineage Assignment and High Resolution Diversity Studies in Trypanosoma cruzi |
dc.creator.none.fl_str_mv |
Yeo, Matthew Mauricio, Isabel L. Messenger, Loisa A. Lewis, Michael D. Llewellyn, Martin S. Acosta, Nidia Bhattacharyya, Tapan Diosque, Patricio Carrasco, Hernán J. Miles, Michael A. |
author |
Yeo, Matthew |
author_facet |
Yeo, Matthew Mauricio, Isabel L. Messenger, Loisa A. Lewis, Michael D. Llewellyn, Martin S. Acosta, Nidia Bhattacharyya, Tapan Diosque, Patricio Carrasco, Hernán J. Miles, Michael A. |
author_role |
author |
author2 |
Mauricio, Isabel L. Messenger, Loisa A. Lewis, Michael D. Llewellyn, Martin S. Acosta, Nidia Bhattacharyya, Tapan Diosque, Patricio Carrasco, Hernán J. Miles, Michael A. |
author2_role |
author author author author author author author author author |
dc.subject.none.fl_str_mv |
MLST TYPING TRYPANOSOMA CRUZI |
topic |
MLST TYPING TRYPANOSOMA CRUZI |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
PLoS Negl Trop Dis. 2011 Jun;5(6):e1049. Epub 2011 Jun 21. Multilocus sequence typing (MLST) for lineage assignment and high resolution diversity studies in Trypanosoma cruzi. Yeo M, Mauricio IL, Messenger LA, Lewis MD, Llewellyn MS, Acosta N, Bhattacharyya T, Diosque P, Carrasco HJ, Miles MA. Source London School of Hygiene and Tropical Medicine, London, United Kingdom. Matthew.yeo@lshtm.ac.uk Abstract BACKGROUND: Multilocus sequence typing (MLST) is a powerful and highly discriminatory method for analysing pathogen population structure and epidemiology. Trypanosoma cruzi, the protozoan agent of American trypanosomiasis (Chagas disease), has remarkable genetic and ecological diversity. A standardised MLST protocol that is suitable for assignment of T. cruzi isolates to genetic lineage and for higher resolution diversity studies has not been developed. METHODOLOGY/PRINCIPAL FINDINGS: We have sequenced and diplotyped nine single copy housekeeping genes and assessed their value as part of a systematic MLST scheme for T. cruzi. A minimum panel of four MLST targets (Met-III, RB19, TcGPXII, and DHFR-TS) was shown to provide unambiguous assignment of isolates to the six known T. cruzi lineages (Discrete Typing Units, DTUs TcI-TcVI). In addition, we recommend six MLST targets (Met-II, Met-III, RB19, TcMPX, DHFR-TS, and TR) for more in depth diversity studies on the basis that diploid sequence typing (DST) with this expanded panel distinguished 38 out of 39 reference isolates. Phylogenetic analysis implies a subdivision between North and South American TcIV isolates. Single Nucleotide Polymorphism (SNP) data revealed high levels of heterozygosity among DTUs TcI, TcIII, TcIV and, for three targets, putative corresponding homozygous and heterozygous loci within DTUs TcI and TcIII. Furthermore, individual gene trees gave incongruent topologies at inter- and intra-DTU levels, inconsistent with a model of strict clonality. CONCLUSIONS/SIGNIFICANCE: We demonstrate the value of systematic MLST diplotyping for describing inter-DTU relationships and for higher resolution diversity studies of T. cruzi, including presence of recombination events. The high levels of heterozygosity will facilitate future population genetics analysis based on MLST haplotypes. Fil: Yeo, Matthew. London School of Hygiene and Tropical Medicine; Reino Unido Fil: Mauricio, Isabel L.. Instituto de Higiene e Medicina Tropical; Portugal Fil: Messenger, Loisa A.. London School of Hygiene and Tropical Medicine; Reino Unido Fil: Lewis, Michael D.. London School of Hygiene and Tropical Medicine; Reino Unido Fil: Llewellyn, Martin S.. London School of Hygiene and Tropical Medicine; Reino Unido Fil: Acosta, Nidia. London School of Hygiene and Tropical Medicine; Reino Unido. Universidad Nacional de Asuncion; Paraguay Fil: Bhattacharyya, Tapan. London School of Hygiene and Tropical Medicine; Reino Unido Fil: Diosque, Patricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Cs.de la Salud. Instituto de Patología Experimental; Argentina Fil: Carrasco, Hernán J.. Universidad Central de Venezuela; Venezuela Fil: Miles, Michael A.. London School of Hygiene and Tropical Medicine; Reino Unido |
description |
PLoS Negl Trop Dis. 2011 Jun;5(6):e1049. Epub 2011 Jun 21. Multilocus sequence typing (MLST) for lineage assignment and high resolution diversity studies in Trypanosoma cruzi. Yeo M, Mauricio IL, Messenger LA, Lewis MD, Llewellyn MS, Acosta N, Bhattacharyya T, Diosque P, Carrasco HJ, Miles MA. Source London School of Hygiene and Tropical Medicine, London, United Kingdom. Matthew.yeo@lshtm.ac.uk Abstract BACKGROUND: Multilocus sequence typing (MLST) is a powerful and highly discriminatory method for analysing pathogen population structure and epidemiology. Trypanosoma cruzi, the protozoan agent of American trypanosomiasis (Chagas disease), has remarkable genetic and ecological diversity. A standardised MLST protocol that is suitable for assignment of T. cruzi isolates to genetic lineage and for higher resolution diversity studies has not been developed. METHODOLOGY/PRINCIPAL FINDINGS: We have sequenced and diplotyped nine single copy housekeeping genes and assessed their value as part of a systematic MLST scheme for T. cruzi. A minimum panel of four MLST targets (Met-III, RB19, TcGPXII, and DHFR-TS) was shown to provide unambiguous assignment of isolates to the six known T. cruzi lineages (Discrete Typing Units, DTUs TcI-TcVI). In addition, we recommend six MLST targets (Met-II, Met-III, RB19, TcMPX, DHFR-TS, and TR) for more in depth diversity studies on the basis that diploid sequence typing (DST) with this expanded panel distinguished 38 out of 39 reference isolates. Phylogenetic analysis implies a subdivision between North and South American TcIV isolates. Single Nucleotide Polymorphism (SNP) data revealed high levels of heterozygosity among DTUs TcI, TcIII, TcIV and, for three targets, putative corresponding homozygous and heterozygous loci within DTUs TcI and TcIII. Furthermore, individual gene trees gave incongruent topologies at inter- and intra-DTU levels, inconsistent with a model of strict clonality. CONCLUSIONS/SIGNIFICANCE: We demonstrate the value of systematic MLST diplotyping for describing inter-DTU relationships and for higher resolution diversity studies of T. cruzi, including presence of recombination events. The high levels of heterozygosity will facilitate future population genetics analysis based on MLST haplotypes. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-06-21 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/16813 Yeo, Matthew; Mauricio, Isabel L.; Messenger, Loisa A.; Lewis, Michael D.; Llewellyn, Martin S.; et al.; Multilocus Sequence Typing (MLST) for Lineage Assignment and High Resolution Diversity Studies in Trypanosoma cruzi; Public Library Of Science; Neglected Tropical Diseases; 5; 6; 21-6-2011; 1049-1049 1935-2735 |
url |
http://hdl.handle.net/11336/16813 |
identifier_str_mv |
Yeo, Matthew; Mauricio, Isabel L.; Messenger, Loisa A.; Lewis, Michael D.; Llewellyn, Martin S.; et al.; Multilocus Sequence Typing (MLST) for Lineage Assignment and High Resolution Diversity Studies in Trypanosoma cruzi; Public Library Of Science; Neglected Tropical Diseases; 5; 6; 21-6-2011; 1049-1049 1935-2735 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0001049 info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0001049 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Of Science |
publisher.none.fl_str_mv |
Public Library Of Science |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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