Dairy bacteria remove in vitro dietary lectins with toxic effects on colonic cells

Autores
Zarate, Gabriela del Valle; Perez Chaia, Adriana Beatriz
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aims: To assess in vitro the ability of some dairy bacteria to bind concanavalin A (Con A), peanut agglutinin (PNA) and jacalin (AIL), preventing their toxicity on mouse intestinal epithelial cells (IEC). Methods and Results: Con A and AIL reduced significantly IEC viability in vitro, as determined by Trypan Blue dye exclusion or by propidium iodide/fluorescein diacetate/Hoescht staining. Different strains of dairy bacteria were able to remove lectins from the media. Two strains were subjected to treatments used to remove S-layer, cell wall proteins, polysaccharides and lectin-like adhesins. They were then assayed for the ability to bind dietary lectins and reduce toxicity against IEC and to adhere to IEC after interaction with lectins. Con A and AIL were removed by Propionibacterium acidipropionici and Propionibacterium freudenreichii by binding with specific sugar moieties on the bacterial surface. Removal of lectins by bacteria impaired IEC protection. Adhesion of P. acidipropionici to IEC was reduced but not abolished after binding Con A or AIL. Conclusions: Removal of Con A or AIL by dairy propionibacteria was effective to avoid the toxic effect against colonic cells in vitro. Significance and Impact of the Study: Consumption of foods containing these bacteria would be a tool to protect the intestinal epithelia.
Fil: Zarate, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Perez Chaia, Adriana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Materia
Adhesion
Colonocytes
Cytotoxicity
Lectins
Propionibacteria
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/54836
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/54836
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Dairy bacteria remove in vitro dietary lectins with toxic effects on colonic cellsZarate, Gabriela del VallePerez Chaia, Adriana BeatrizAdhesionColonocytesCytotoxicityLectinsPropionibacteriahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Aims: To assess in vitro the ability of some dairy bacteria to bind concanavalin A (Con A), peanut agglutinin (PNA) and jacalin (AIL), preventing their toxicity on mouse intestinal epithelial cells (IEC). Methods and Results: Con A and AIL reduced significantly IEC viability in vitro, as determined by Trypan Blue dye exclusion or by propidium iodide/fluorescein diacetate/Hoescht staining. Different strains of dairy bacteria were able to remove lectins from the media. Two strains were subjected to treatments used to remove S-layer, cell wall proteins, polysaccharides and lectin-like adhesins. They were then assayed for the ability to bind dietary lectins and reduce toxicity against IEC and to adhere to IEC after interaction with lectins. Con A and AIL were removed by Propionibacterium acidipropionici and Propionibacterium freudenreichii by binding with specific sugar moieties on the bacterial surface. Removal of lectins by bacteria impaired IEC protection. Adhesion of P. acidipropionici to IEC was reduced but not abolished after binding Con A or AIL. Conclusions: Removal of Con A or AIL by dairy propionibacteria was effective to avoid the toxic effect against colonic cells in vitro. Significance and Impact of the Study: Consumption of foods containing these bacteria would be a tool to protect the intestinal epithelia.Fil: Zarate, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Perez Chaia, Adriana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaWiley Blackwell Publishing, Inc2009-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54836Zarate, Gabriela del Valle; Perez Chaia, Adriana Beatriz; Dairy bacteria remove in vitro dietary lectins with toxic effects on colonic cells; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 106; 3; 3-2009; 1050-10571364-50721365-2672CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2672.2008.04077.xinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2672.2008.04077.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:44:07Zoai:ri.conicet.gov.ar:11336/54836instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:44:08.084CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dairy bacteria remove in vitro dietary lectins with toxic effects on colonic cells
title Dairy bacteria remove in vitro dietary lectins with toxic effects on colonic cells
spellingShingle Dairy bacteria remove in vitro dietary lectins with toxic effects on colonic cells
Zarate, Gabriela del Valle
Adhesion
Colonocytes
Cytotoxicity
Lectins
Propionibacteria
title_short Dairy bacteria remove in vitro dietary lectins with toxic effects on colonic cells
title_full Dairy bacteria remove in vitro dietary lectins with toxic effects on colonic cells
title_fullStr Dairy bacteria remove in vitro dietary lectins with toxic effects on colonic cells
title_full_unstemmed Dairy bacteria remove in vitro dietary lectins with toxic effects on colonic cells
title_sort Dairy bacteria remove in vitro dietary lectins with toxic effects on colonic cells
dc.creator.none.fl_str_mv Zarate, Gabriela del Valle
Perez Chaia, Adriana Beatriz
author Zarate, Gabriela del Valle
author_facet Zarate, Gabriela del Valle
Perez Chaia, Adriana Beatriz
author_role author
author2 Perez Chaia, Adriana Beatriz
author2_role author
dc.subject.none.fl_str_mv Adhesion
Colonocytes
Cytotoxicity
Lectins
Propionibacteria
topic Adhesion
Colonocytes
Cytotoxicity
Lectins
Propionibacteria
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Aims: To assess in vitro the ability of some dairy bacteria to bind concanavalin A (Con A), peanut agglutinin (PNA) and jacalin (AIL), preventing their toxicity on mouse intestinal epithelial cells (IEC). Methods and Results: Con A and AIL reduced significantly IEC viability in vitro, as determined by Trypan Blue dye exclusion or by propidium iodide/fluorescein diacetate/Hoescht staining. Different strains of dairy bacteria were able to remove lectins from the media. Two strains were subjected to treatments used to remove S-layer, cell wall proteins, polysaccharides and lectin-like adhesins. They were then assayed for the ability to bind dietary lectins and reduce toxicity against IEC and to adhere to IEC after interaction with lectins. Con A and AIL were removed by Propionibacterium acidipropionici and Propionibacterium freudenreichii by binding with specific sugar moieties on the bacterial surface. Removal of lectins by bacteria impaired IEC protection. Adhesion of P. acidipropionici to IEC was reduced but not abolished after binding Con A or AIL. Conclusions: Removal of Con A or AIL by dairy propionibacteria was effective to avoid the toxic effect against colonic cells in vitro. Significance and Impact of the Study: Consumption of foods containing these bacteria would be a tool to protect the intestinal epithelia.
Fil: Zarate, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Perez Chaia, Adriana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
description Aims: To assess in vitro the ability of some dairy bacteria to bind concanavalin A (Con A), peanut agglutinin (PNA) and jacalin (AIL), preventing their toxicity on mouse intestinal epithelial cells (IEC). Methods and Results: Con A and AIL reduced significantly IEC viability in vitro, as determined by Trypan Blue dye exclusion or by propidium iodide/fluorescein diacetate/Hoescht staining. Different strains of dairy bacteria were able to remove lectins from the media. Two strains were subjected to treatments used to remove S-layer, cell wall proteins, polysaccharides and lectin-like adhesins. They were then assayed for the ability to bind dietary lectins and reduce toxicity against IEC and to adhere to IEC after interaction with lectins. Con A and AIL were removed by Propionibacterium acidipropionici and Propionibacterium freudenreichii by binding with specific sugar moieties on the bacterial surface. Removal of lectins by bacteria impaired IEC protection. Adhesion of P. acidipropionici to IEC was reduced but not abolished after binding Con A or AIL. Conclusions: Removal of Con A or AIL by dairy propionibacteria was effective to avoid the toxic effect against colonic cells in vitro. Significance and Impact of the Study: Consumption of foods containing these bacteria would be a tool to protect the intestinal epithelia.
publishDate 2009
dc.date.none.fl_str_mv 2009-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/54836
Zarate, Gabriela del Valle; Perez Chaia, Adriana Beatriz; Dairy bacteria remove in vitro dietary lectins with toxic effects on colonic cells; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 106; 3; 3-2009; 1050-1057
1364-5072
1365-2672
CONICET Digital
CONICET
url http://hdl.handle.net/11336/54836
identifier_str_mv Zarate, Gabriela del Valle; Perez Chaia, Adriana Beatriz; Dairy bacteria remove in vitro dietary lectins with toxic effects on colonic cells; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 106; 3; 3-2009; 1050-1057
1364-5072
1365-2672
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2672.2008.04077.x
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2672.2008.04077.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613388874809344
score 13.070432

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