Estudio de microbiomas en ámbitos médicos-asistenciales por microscopía electrónica de barrido

Autores
D'arpino, María Cecilia; Galván, Fátima Silvina; Alvarado, Natalia Noelia; Marranzino, Gabriela; Martinez, L.; Albarracín, Virginia Helena
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The identification of microbiomes present in the health field is extremely important given the close relationship with human diseases. The aim of this study was to investigate the composition of microorganisms on the laboratory surfaces of the Central Blood Bank "Dr. César Guerra", (PRIS-SI.PRO.SA). Duplicate samples from the countertops, air conditioners and the equipment on the Production, Distribution and Molecular Biology services were taken using paper tape and swabs. Plating was carried out in LB pH7 culture medium containing Cycloheximide (CH) and Cyclohehimide/ Nalidixic Acid (CH / NA) antibiotics. Both the paper tape and the colonies obtained from the cultures were subjected to Scanning Electron Microscopy (SEM). Sampling using paper tape allowed the presence of microbial biofilms to be detected in the internal part of the Production service centrifuge, in the Distribution service platelet shaker and in the Molecular Biology service countertops. They presented a complex three-dimensional organization characterized by microorganisms of different morphology arranged in layers immersed in abundant extracellular material. The tape analysis also revealed the presence of isolated bacteria (cocci and bacilli) or the formation of small groups of them at the different sampling sites. The cultures allowed isolating predominant microorganisms from countertops, air conditioners and equipment. A total of 45 colonies (gram + and gram -) that exhibited various morphotypes (cocci, bacilli and cocobacilli) were isolated. Using SEM, it was possible to analyze in detail the structure, organization and morphology of the bacteria in culture. Furthermore, it was observed that many colonies established close contacts. The SEM study revealed a wide spectrum of relationships among them. It was possible to analyze the contact points among interacting colonies, revealing morphological changes in the bacteria as well as a large amount of extracellular material at the interaction sites. In addition, the topographic analysis of the colonies showed differences in the conformation of the different sectors in some of them. This work, aimed at analyzing the microbiological communities developed in situ in healthcare settings, proposes high-resolution microscopy techniques as key tools for the study in situ of biofilms on a surface, which study is lacking in our country.
Fil: D'arpino, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Galván, Fátima Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Alvarado, Natalia Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Marranzino, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Martinez, L.. Centro Integral de Microscopía Electrónica; Argentina
Fil: Albarracín, Virginia Helena. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
IV Reunión Conjunta de Sociedades de Biología de la República Argentina
Tucumán
Argentina
Sociedad de Biología de Tucumán
Sociedad de Biología de Cuyo
Sociedad de Biología de Córdoba
Materia
Microbiome
Biofilms
Scanning Electron Microscopy
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/181495
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Estudio de microbiomas en ámbitos médicos-asistenciales por microscopía electrónica de barridoD'arpino, María CeciliaGalván, Fátima SilvinaAlvarado, Natalia NoeliaMarranzino, GabrielaMartinez, L.Albarracín, Virginia HelenaMicrobiomeBiofilmsScanning Electron Microscopyhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The identification of microbiomes present in the health field is extremely important given the close relationship with human diseases. The aim of this study was to investigate the composition of microorganisms on the laboratory surfaces of the Central Blood Bank "Dr. César Guerra", (PRIS-SI.PRO.SA). Duplicate samples from the countertops, air conditioners and the equipment on the Production, Distribution and Molecular Biology services were taken using paper tape and swabs. Plating was carried out in LB pH7 culture medium containing Cycloheximide (CH) and Cyclohehimide/ Nalidixic Acid (CH / NA) antibiotics. Both the paper tape and the colonies obtained from the cultures were subjected to Scanning Electron Microscopy (SEM). Sampling using paper tape allowed the presence of microbial biofilms to be detected in the internal part of the Production service centrifuge, in the Distribution service platelet shaker and in the Molecular Biology service countertops. They presented a complex three-dimensional organization characterized by microorganisms of different morphology arranged in layers immersed in abundant extracellular material. The tape analysis also revealed the presence of isolated bacteria (cocci and bacilli) or the formation of small groups of them at the different sampling sites. The cultures allowed isolating predominant microorganisms from countertops, air conditioners and equipment. A total of 45 colonies (gram + and gram -) that exhibited various morphotypes (cocci, bacilli and cocobacilli) were isolated. Using SEM, it was possible to analyze in detail the structure, organization and morphology of the bacteria in culture. Furthermore, it was observed that many colonies established close contacts. The SEM study revealed a wide spectrum of relationships among them. It was possible to analyze the contact points among interacting colonies, revealing morphological changes in the bacteria as well as a large amount of extracellular material at the interaction sites. In addition, the topographic analysis of the colonies showed differences in the conformation of the different sectors in some of them. This work, aimed at analyzing the microbiological communities developed in situ in healthcare settings, proposes high-resolution microscopy techniques as key tools for the study in situ of biofilms on a surface, which study is lacking in our country.Fil: D'arpino, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Galván, Fátima Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Alvarado, Natalia Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Marranzino, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Martinez, L.. Centro Integral de Microscopía Electrónica; ArgentinaFil: Albarracín, Virginia Helena. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; ArgentinaIV Reunión Conjunta de Sociedades de Biología de la República ArgentinaTucumánArgentinaSociedad de Biología de TucumánSociedad de Biología de CuyoSociedad de Biología de CórdobaSociedad Argentina de Biología2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/181495Estudio de microbiomas en ámbitos médicos-asistenciales por microscopía electrónica de barrido; IV Reunión Conjunta de Sociedades de Biología de la República Argentina; Tucumán; Argentina; 2020; 1-6CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.asobioltuc.com/uploads/archivos/1601503071_TElCUk8gREUgUkVTVU1FTkVTIElWIFJFVU5Jw5NOIENPTkpVTlRBIERFIFNPQ0lFREFERVMgREUgQklPTE9HSUEgLnBkZg==.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:18:36Zoai:ri.conicet.gov.ar:11336/181495instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:18:36.933CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Estudio de microbiomas en ámbitos médicos-asistenciales por microscopía electrónica de barrido
title Estudio de microbiomas en ámbitos médicos-asistenciales por microscopía electrónica de barrido
spellingShingle Estudio de microbiomas en ámbitos médicos-asistenciales por microscopía electrónica de barrido
D'arpino, María Cecilia
Microbiome
Biofilms
Scanning Electron Microscopy
title_short Estudio de microbiomas en ámbitos médicos-asistenciales por microscopía electrónica de barrido
title_full Estudio de microbiomas en ámbitos médicos-asistenciales por microscopía electrónica de barrido
title_fullStr Estudio de microbiomas en ámbitos médicos-asistenciales por microscopía electrónica de barrido
title_full_unstemmed Estudio de microbiomas en ámbitos médicos-asistenciales por microscopía electrónica de barrido
title_sort Estudio de microbiomas en ámbitos médicos-asistenciales por microscopía electrónica de barrido
dc.creator.none.fl_str_mv D'arpino, María Cecilia
Galván, Fátima Silvina
Alvarado, Natalia Noelia
Marranzino, Gabriela
Martinez, L.
Albarracín, Virginia Helena
author D'arpino, María Cecilia
author_facet D'arpino, María Cecilia
Galván, Fátima Silvina
Alvarado, Natalia Noelia
Marranzino, Gabriela
Martinez, L.
Albarracín, Virginia Helena
author_role author
author2 Galván, Fátima Silvina
Alvarado, Natalia Noelia
Marranzino, Gabriela
Martinez, L.
Albarracín, Virginia Helena
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Microbiome
Biofilms
Scanning Electron Microscopy
topic Microbiome
Biofilms
Scanning Electron Microscopy
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The identification of microbiomes present in the health field is extremely important given the close relationship with human diseases. The aim of this study was to investigate the composition of microorganisms on the laboratory surfaces of the Central Blood Bank "Dr. César Guerra", (PRIS-SI.PRO.SA). Duplicate samples from the countertops, air conditioners and the equipment on the Production, Distribution and Molecular Biology services were taken using paper tape and swabs. Plating was carried out in LB pH7 culture medium containing Cycloheximide (CH) and Cyclohehimide/ Nalidixic Acid (CH / NA) antibiotics. Both the paper tape and the colonies obtained from the cultures were subjected to Scanning Electron Microscopy (SEM). Sampling using paper tape allowed the presence of microbial biofilms to be detected in the internal part of the Production service centrifuge, in the Distribution service platelet shaker and in the Molecular Biology service countertops. They presented a complex three-dimensional organization characterized by microorganisms of different morphology arranged in layers immersed in abundant extracellular material. The tape analysis also revealed the presence of isolated bacteria (cocci and bacilli) or the formation of small groups of them at the different sampling sites. The cultures allowed isolating predominant microorganisms from countertops, air conditioners and equipment. A total of 45 colonies (gram + and gram -) that exhibited various morphotypes (cocci, bacilli and cocobacilli) were isolated. Using SEM, it was possible to analyze in detail the structure, organization and morphology of the bacteria in culture. Furthermore, it was observed that many colonies established close contacts. The SEM study revealed a wide spectrum of relationships among them. It was possible to analyze the contact points among interacting colonies, revealing morphological changes in the bacteria as well as a large amount of extracellular material at the interaction sites. In addition, the topographic analysis of the colonies showed differences in the conformation of the different sectors in some of them. This work, aimed at analyzing the microbiological communities developed in situ in healthcare settings, proposes high-resolution microscopy techniques as key tools for the study in situ of biofilms on a surface, which study is lacking in our country.
Fil: D'arpino, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Galván, Fátima Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Alvarado, Natalia Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Marranzino, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Martinez, L.. Centro Integral de Microscopía Electrónica; Argentina
Fil: Albarracín, Virginia Helena. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
IV Reunión Conjunta de Sociedades de Biología de la República Argentina
Tucumán
Argentina
Sociedad de Biología de Tucumán
Sociedad de Biología de Cuyo
Sociedad de Biología de Córdoba
description The identification of microbiomes present in the health field is extremely important given the close relationship with human diseases. The aim of this study was to investigate the composition of microorganisms on the laboratory surfaces of the Central Blood Bank "Dr. César Guerra", (PRIS-SI.PRO.SA). Duplicate samples from the countertops, air conditioners and the equipment on the Production, Distribution and Molecular Biology services were taken using paper tape and swabs. Plating was carried out in LB pH7 culture medium containing Cycloheximide (CH) and Cyclohehimide/ Nalidixic Acid (CH / NA) antibiotics. Both the paper tape and the colonies obtained from the cultures were subjected to Scanning Electron Microscopy (SEM). Sampling using paper tape allowed the presence of microbial biofilms to be detected in the internal part of the Production service centrifuge, in the Distribution service platelet shaker and in the Molecular Biology service countertops. They presented a complex three-dimensional organization characterized by microorganisms of different morphology arranged in layers immersed in abundant extracellular material. The tape analysis also revealed the presence of isolated bacteria (cocci and bacilli) or the formation of small groups of them at the different sampling sites. The cultures allowed isolating predominant microorganisms from countertops, air conditioners and equipment. A total of 45 colonies (gram + and gram -) that exhibited various morphotypes (cocci, bacilli and cocobacilli) were isolated. Using SEM, it was possible to analyze in detail the structure, organization and morphology of the bacteria in culture. Furthermore, it was observed that many colonies established close contacts. The SEM study revealed a wide spectrum of relationships among them. It was possible to analyze the contact points among interacting colonies, revealing morphological changes in the bacteria as well as a large amount of extracellular material at the interaction sites. In addition, the topographic analysis of the colonies showed differences in the conformation of the different sectors in some of them. This work, aimed at analyzing the microbiological communities developed in situ in healthcare settings, proposes high-resolution microscopy techniques as key tools for the study in situ of biofilms on a surface, which study is lacking in our country.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/181495
Estudio de microbiomas en ámbitos médicos-asistenciales por microscopía electrónica de barrido; IV Reunión Conjunta de Sociedades de Biología de la República Argentina; Tucumán; Argentina; 2020; 1-6
CONICET Digital
CONICET
url http://hdl.handle.net/11336/181495
identifier_str_mv Estudio de microbiomas en ámbitos médicos-asistenciales por microscopía electrónica de barrido; IV Reunión Conjunta de Sociedades de Biología de la República Argentina; Tucumán; Argentina; 2020; 1-6
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.asobioltuc.com/uploads/archivos/1601503071_TElCUk8gREUgUkVTVU1FTkVTIElWIFJFVU5Jw5NOIENPTkpVTlRBIERFIFNPQ0lFREFERVMgREUgQklPTE9HSUEgLnBkZg==.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Biología
publisher.none.fl_str_mv Sociedad Argentina de Biología
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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