Characterization of β-Glucosidase produced by the white rot fungus Flammulina velutipes

Autores
Mallerman, Julieta; Papinutti, Victor Leandro; Levin, Laura Noemi
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
β-glucosidase production by the white rot fungus Flammulina velutipes CFK 3111 was evaluated using different carbon and nitrogen sources under submerged fermentation. Maximal extracellular enzyme production was 1.6 U/mL corresponding to a culture grown in sucrose 40 g/L and asparagine 10 g/L. High production yield was also obtained with glucose 10 g/L and asparagine 4 g/L medium (0.5 U/mL). Parameters affecting enzyme activity were studied using p-nitro-phenyl-β-D-glucopyranoside as substrate. Optimal activity was found at 50°C and pHs 5.0 to 6.0. Under these conditions, β-glucosidase retained 25% of its initial activity after 12 h of incubation and exhibited a half-life of 5 h. The addition of MgCl2, urea and ethanol enhanced β-glucosidase activity up to 47% while the reactives FeCl2, CuSO4, Cd(NO3)2 and cetyltrimethylammonium bromide inflicted a strong inhibitory effect. Glucose and cellobiose showed also an inhibitory effect on β-glucosidase activity in a concentration dependent manner. The enzyme had an estimated molecular mass of 75 kDa. To the best of our knowledge, F. velutipes CFK 3111 β -glucosidase production is amongst the highest reported up to date, in a basidiomycetous fungus.
Fil: Mallerman, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botanica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botanica; Argentina
Fil: Papinutti, Victor Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botanica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botanica; Argentina
Fil: Levin, Laura Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botanica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botanica; Argentina
Materia
B-Glucosidase
Flammulina Velutipes
Submerged Fermentation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16733
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Characterization of β-Glucosidase produced by the white rot fungus Flammulina velutipesMallerman, JulietaPapinutti, Victor LeandroLevin, Laura NoemiB-GlucosidaseFlammulina VelutipesSubmerged Fermentationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1β-glucosidase production by the white rot fungus Flammulina velutipes CFK 3111 was evaluated using different carbon and nitrogen sources under submerged fermentation. Maximal extracellular enzyme production was 1.6 U/mL corresponding to a culture grown in sucrose 40 g/L and asparagine 10 g/L. High production yield was also obtained with glucose 10 g/L and asparagine 4 g/L medium (0.5 U/mL). Parameters affecting enzyme activity were studied using p-nitro-phenyl-β-D-glucopyranoside as substrate. Optimal activity was found at 50°C and pHs 5.0 to 6.0. Under these conditions, β-glucosidase retained 25% of its initial activity after 12 h of incubation and exhibited a half-life of 5 h. The addition of MgCl2, urea and ethanol enhanced β-glucosidase activity up to 47% while the reactives FeCl2, CuSO4, Cd(NO3)2 and cetyltrimethylammonium bromide inflicted a strong inhibitory effect. Glucose and cellobiose showed also an inhibitory effect on β-glucosidase activity in a concentration dependent manner. The enzyme had an estimated molecular mass of 75 kDa. To the best of our knowledge, F. velutipes CFK 3111 β -glucosidase production is amongst the highest reported up to date, in a basidiomycetous fungus.Fil: Mallerman, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botanica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botanica; ArgentinaFil: Papinutti, Victor Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botanica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botanica; ArgentinaFil: Levin, Laura Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botanica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botanica; ArgentinaKorean Society Microbiology2014-09-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16733Mallerman, Julieta; Papinutti, Victor Leandro; Levin, Laura Noemi; Characterization of β-Glucosidase produced by the white rot fungus Flammulina velutipes; Korean Society Microbiology; Journal Of Microbiology And Biotechnology; 25; 1; 4-9-2014; 57-651598-2467enginfo:eu-repo/semantics/altIdentifier/url/http://www.jmb.or.kr/journal/view.html?doi=10.4014/jmb.1401.01045info:eu-repo/semantics/altIdentifier/doi/10.4014/jmb.1401.01045info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:25Zoai:ri.conicet.gov.ar:11336/16733instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:26.956CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of β-Glucosidase produced by the white rot fungus Flammulina velutipes
title Characterization of β-Glucosidase produced by the white rot fungus Flammulina velutipes
spellingShingle Characterization of β-Glucosidase produced by the white rot fungus Flammulina velutipes
Mallerman, Julieta
B-Glucosidase
Flammulina Velutipes
Submerged Fermentation
title_short Characterization of β-Glucosidase produced by the white rot fungus Flammulina velutipes
title_full Characterization of β-Glucosidase produced by the white rot fungus Flammulina velutipes
title_fullStr Characterization of β-Glucosidase produced by the white rot fungus Flammulina velutipes
title_full_unstemmed Characterization of β-Glucosidase produced by the white rot fungus Flammulina velutipes
title_sort Characterization of β-Glucosidase produced by the white rot fungus Flammulina velutipes
dc.creator.none.fl_str_mv Mallerman, Julieta
Papinutti, Victor Leandro
Levin, Laura Noemi
author Mallerman, Julieta
author_facet Mallerman, Julieta
Papinutti, Victor Leandro
Levin, Laura Noemi
author_role author
author2 Papinutti, Victor Leandro
Levin, Laura Noemi
author2_role author
author
dc.subject.none.fl_str_mv B-Glucosidase
Flammulina Velutipes
Submerged Fermentation
topic B-Glucosidase
Flammulina Velutipes
Submerged Fermentation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv β-glucosidase production by the white rot fungus Flammulina velutipes CFK 3111 was evaluated using different carbon and nitrogen sources under submerged fermentation. Maximal extracellular enzyme production was 1.6 U/mL corresponding to a culture grown in sucrose 40 g/L and asparagine 10 g/L. High production yield was also obtained with glucose 10 g/L and asparagine 4 g/L medium (0.5 U/mL). Parameters affecting enzyme activity were studied using p-nitro-phenyl-β-D-glucopyranoside as substrate. Optimal activity was found at 50°C and pHs 5.0 to 6.0. Under these conditions, β-glucosidase retained 25% of its initial activity after 12 h of incubation and exhibited a half-life of 5 h. The addition of MgCl2, urea and ethanol enhanced β-glucosidase activity up to 47% while the reactives FeCl2, CuSO4, Cd(NO3)2 and cetyltrimethylammonium bromide inflicted a strong inhibitory effect. Glucose and cellobiose showed also an inhibitory effect on β-glucosidase activity in a concentration dependent manner. The enzyme had an estimated molecular mass of 75 kDa. To the best of our knowledge, F. velutipes CFK 3111 β -glucosidase production is amongst the highest reported up to date, in a basidiomycetous fungus.
Fil: Mallerman, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botanica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botanica; Argentina
Fil: Papinutti, Victor Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botanica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botanica; Argentina
Fil: Levin, Laura Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botanica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botanica; Argentina
description β-glucosidase production by the white rot fungus Flammulina velutipes CFK 3111 was evaluated using different carbon and nitrogen sources under submerged fermentation. Maximal extracellular enzyme production was 1.6 U/mL corresponding to a culture grown in sucrose 40 g/L and asparagine 10 g/L. High production yield was also obtained with glucose 10 g/L and asparagine 4 g/L medium (0.5 U/mL). Parameters affecting enzyme activity were studied using p-nitro-phenyl-β-D-glucopyranoside as substrate. Optimal activity was found at 50°C and pHs 5.0 to 6.0. Under these conditions, β-glucosidase retained 25% of its initial activity after 12 h of incubation and exhibited a half-life of 5 h. The addition of MgCl2, urea and ethanol enhanced β-glucosidase activity up to 47% while the reactives FeCl2, CuSO4, Cd(NO3)2 and cetyltrimethylammonium bromide inflicted a strong inhibitory effect. Glucose and cellobiose showed also an inhibitory effect on β-glucosidase activity in a concentration dependent manner. The enzyme had an estimated molecular mass of 75 kDa. To the best of our knowledge, F. velutipes CFK 3111 β -glucosidase production is amongst the highest reported up to date, in a basidiomycetous fungus.
publishDate 2014
dc.date.none.fl_str_mv 2014-09-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16733
Mallerman, Julieta; Papinutti, Victor Leandro; Levin, Laura Noemi; Characterization of β-Glucosidase produced by the white rot fungus Flammulina velutipes; Korean Society Microbiology; Journal Of Microbiology And Biotechnology; 25; 1; 4-9-2014; 57-65
1598-2467
url http://hdl.handle.net/11336/16733
identifier_str_mv Mallerman, Julieta; Papinutti, Victor Leandro; Levin, Laura Noemi; Characterization of β-Glucosidase produced by the white rot fungus Flammulina velutipes; Korean Society Microbiology; Journal Of Microbiology And Biotechnology; 25; 1; 4-9-2014; 57-65
1598-2467
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.jmb.or.kr/journal/view.html?doi=10.4014/jmb.1401.01045
info:eu-repo/semantics/altIdentifier/doi/10.4014/jmb.1401.01045
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Korean Society Microbiology
publisher.none.fl_str_mv Korean Society Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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