Bioactive action of b-glucosidase enzyme of Bifidobacterium longum upon isoflavone glucosides present in soymilk

Autores
Marazza, Jose Antonio; Nazareno, Mónica Azucena; Savoy, Graciela; Garro, Marisa Selva
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bioconversion of isoflavone glucosides and antioxidant activity by probiotic strain (Bifidobacterium longum)during soymilk fermentation was investigated, as well as partial characterisation of the produced enzyme b-glucosidase. The enzyme has higher affinity for genistin than for other substrates assayed. Maximum activity occurred at 42 °C and at pH 6.0; keeping 70?80% of activity for 60 days stored at low temperatures. Bifidobacterium longum grew well in soymilk (8.26 log CFU Ml_1 and pH of 3.9 at 24 h) and were produced in good quantities of organic acids. High hydrolysis degree of isoflavone glucosides (81.2%) was observed at 24 h. Enhancements in bioactivity were assessed in fermented soymilk by monitoring the radical-scavenging activity, antioxidant activity and DNA protective action. The use of probiotic Bifidobacterium strain as b-glucosidase producer increased bioactive isoflavone content and demonstrated that this enzyme plays a key role in the bioavailability of soymilk isoflavones, reducing the bioconversion time compared to other studies.
Fil: Marazza, Jose Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia Para Lactobacilos (i); Argentina
Fil: Nazareno, Mónica Azucena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina
Fil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia Para Lactobacilos (i); Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Cátedra de Microbiología Superior; Argentina
Fil: Garro, Marisa Selva. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia Para Lactobacilos (i); Argentina
Materia
Antioxidant Activity
Isoflavones
Bifidobacterium
Soymilk Fermentation
B-Glucosidase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16483
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/16483
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bioactive action of b-glucosidase enzyme of Bifidobacterium longum upon isoflavone glucosides present in soymilkMarazza, Jose AntonioNazareno, Mónica AzucenaSavoy, GracielaGarro, Marisa SelvaAntioxidant ActivityIsoflavonesBifidobacteriumSoymilk FermentationB-Glucosidasehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Bioconversion of isoflavone glucosides and antioxidant activity by probiotic strain (Bifidobacterium longum)during soymilk fermentation was investigated, as well as partial characterisation of the produced enzyme b-glucosidase. The enzyme has higher affinity for genistin than for other substrates assayed. Maximum activity occurred at 42 °C and at pH 6.0; keeping 70?80% of activity for 60 days stored at low temperatures. Bifidobacterium longum grew well in soymilk (8.26 log CFU Ml_1 and pH of 3.9 at 24 h) and were produced in good quantities of organic acids. High hydrolysis degree of isoflavone glucosides (81.2%) was observed at 24 h. Enhancements in bioactivity were assessed in fermented soymilk by monitoring the radical-scavenging activity, antioxidant activity and DNA protective action. The use of probiotic Bifidobacterium strain as b-glucosidase producer increased bioactive isoflavone content and demonstrated that this enzyme plays a key role in the bioavailability of soymilk isoflavones, reducing the bioconversion time compared to other studies.Fil: Marazza, Jose Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia Para Lactobacilos (i); ArgentinaFil: Nazareno, Mónica Azucena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; ArgentinaFil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia Para Lactobacilos (i); Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Cátedra de Microbiología Superior; ArgentinaFil: Garro, Marisa Selva. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia Para Lactobacilos (i); ArgentinaWiley Blackwell Publishing, Inc2013-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16483Marazza, Jose Antonio; Nazareno, Mónica Azucena; Savoy, Graciela; Garro, Marisa Selva; Bioactive action of b-glucosidase enzyme of Bifidobacterium longum upon isoflavone glucosides present in soymilk; Wiley Blackwell Publishing, Inc; International Journal Of Food Science And Technology; 48; 12; 12-2013; 2480-24890950-54231365-2621enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/ijfs.12239/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1111/ijfs.12239info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:44Zoai:ri.conicet.gov.ar:11336/16483instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:44.491CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bioactive action of b-glucosidase enzyme of Bifidobacterium longum upon isoflavone glucosides present in soymilk
title Bioactive action of b-glucosidase enzyme of Bifidobacterium longum upon isoflavone glucosides present in soymilk
spellingShingle Bioactive action of b-glucosidase enzyme of Bifidobacterium longum upon isoflavone glucosides present in soymilk
Marazza, Jose Antonio
Antioxidant Activity
Isoflavones
Bifidobacterium
Soymilk Fermentation
B-Glucosidase
title_short Bioactive action of b-glucosidase enzyme of Bifidobacterium longum upon isoflavone glucosides present in soymilk
title_full Bioactive action of b-glucosidase enzyme of Bifidobacterium longum upon isoflavone glucosides present in soymilk
title_fullStr Bioactive action of b-glucosidase enzyme of Bifidobacterium longum upon isoflavone glucosides present in soymilk
title_full_unstemmed Bioactive action of b-glucosidase enzyme of Bifidobacterium longum upon isoflavone glucosides present in soymilk
title_sort Bioactive action of b-glucosidase enzyme of Bifidobacterium longum upon isoflavone glucosides present in soymilk
dc.creator.none.fl_str_mv Marazza, Jose Antonio
Nazareno, Mónica Azucena
Savoy, Graciela
Garro, Marisa Selva
author Marazza, Jose Antonio
author_facet Marazza, Jose Antonio
Nazareno, Mónica Azucena
Savoy, Graciela
Garro, Marisa Selva
author_role author
author2 Nazareno, Mónica Azucena
Savoy, Graciela
Garro, Marisa Selva
author2_role author
author
author
dc.subject.none.fl_str_mv Antioxidant Activity
Isoflavones
Bifidobacterium
Soymilk Fermentation
B-Glucosidase
topic Antioxidant Activity
Isoflavones
Bifidobacterium
Soymilk Fermentation
B-Glucosidase
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Bioconversion of isoflavone glucosides and antioxidant activity by probiotic strain (Bifidobacterium longum)during soymilk fermentation was investigated, as well as partial characterisation of the produced enzyme b-glucosidase. The enzyme has higher affinity for genistin than for other substrates assayed. Maximum activity occurred at 42 °C and at pH 6.0; keeping 70?80% of activity for 60 days stored at low temperatures. Bifidobacterium longum grew well in soymilk (8.26 log CFU Ml_1 and pH of 3.9 at 24 h) and were produced in good quantities of organic acids. High hydrolysis degree of isoflavone glucosides (81.2%) was observed at 24 h. Enhancements in bioactivity were assessed in fermented soymilk by monitoring the radical-scavenging activity, antioxidant activity and DNA protective action. The use of probiotic Bifidobacterium strain as b-glucosidase producer increased bioactive isoflavone content and demonstrated that this enzyme plays a key role in the bioavailability of soymilk isoflavones, reducing the bioconversion time compared to other studies.
Fil: Marazza, Jose Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia Para Lactobacilos (i); Argentina
Fil: Nazareno, Mónica Azucena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina
Fil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia Para Lactobacilos (i); Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Cátedra de Microbiología Superior; Argentina
Fil: Garro, Marisa Selva. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia Para Lactobacilos (i); Argentina
description Bioconversion of isoflavone glucosides and antioxidant activity by probiotic strain (Bifidobacterium longum)during soymilk fermentation was investigated, as well as partial characterisation of the produced enzyme b-glucosidase. The enzyme has higher affinity for genistin than for other substrates assayed. Maximum activity occurred at 42 °C and at pH 6.0; keeping 70?80% of activity for 60 days stored at low temperatures. Bifidobacterium longum grew well in soymilk (8.26 log CFU Ml_1 and pH of 3.9 at 24 h) and were produced in good quantities of organic acids. High hydrolysis degree of isoflavone glucosides (81.2%) was observed at 24 h. Enhancements in bioactivity were assessed in fermented soymilk by monitoring the radical-scavenging activity, antioxidant activity and DNA protective action. The use of probiotic Bifidobacterium strain as b-glucosidase producer increased bioactive isoflavone content and demonstrated that this enzyme plays a key role in the bioavailability of soymilk isoflavones, reducing the bioconversion time compared to other studies.
publishDate 2013
dc.date.none.fl_str_mv 2013-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16483
Marazza, Jose Antonio; Nazareno, Mónica Azucena; Savoy, Graciela; Garro, Marisa Selva; Bioactive action of b-glucosidase enzyme of Bifidobacterium longum upon isoflavone glucosides present in soymilk; Wiley Blackwell Publishing, Inc; International Journal Of Food Science And Technology; 48; 12; 12-2013; 2480-2489
0950-5423
1365-2621
url http://hdl.handle.net/11336/16483
identifier_str_mv Marazza, Jose Antonio; Nazareno, Mónica Azucena; Savoy, Graciela; Garro, Marisa Selva; Bioactive action of b-glucosidase enzyme of Bifidobacterium longum upon isoflavone glucosides present in soymilk; Wiley Blackwell Publishing, Inc; International Journal Of Food Science And Technology; 48; 12; 12-2013; 2480-2489
0950-5423
1365-2621
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/ijfs.12239/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1111/ijfs.12239
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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