Isolation and molecular characterization of Shewanella sp. G5, a producer of cold-active β -D-glucosidases

Autores
Cristobal, Hector Antonio; Breccia, Javier Dario; Abate, Carlos Mauricio
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
β ‐Glucosidase is a highly desired glycosidase, especially for hydrolysis of glycoconjugated precursors in musts and wines for the release of active aromatic compounds. A Shewanella sp. G5 strain was isolated from the intestinal content of benthonic organism (Munida subrrugosa) from different coastal areas of the Beagle Channel, Tierra del Fuego (Argentina). This marine bacterium was able to grow at a temperature range between 4 to 20 °C using different β ‐glycoside substrates, such as cellobiose, as carbon source. In this work, the Shewanella sp. G5 strain exhibited high β ‐glucosidase activity on plate at low temperature (4 and 20 °C). Two genes encoding different cold‐active β ‐glucosidases were amplified and sequenced and the nucleotide sequences were submitted to the GenBank. 16S rDNA and gyrB gene sequences were used for the molecular characterization of Shewanella sp. G5.
Fil: Cristobal, Hector Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Breccia, Javier Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Abate, Carlos Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Materia
Psychrotolerant
Â-Glucosidase
Gyrb
16s Rdna
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/43472
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Isolation and molecular characterization of Shewanella sp. G5, a producer of cold-active β -D-glucosidasesCristobal, Hector AntonioBreccia, Javier DarioAbate, Carlos MauricioPsychrotolerantÂ-GlucosidaseGyrb16s Rdnahttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2β ‐Glucosidase is a highly desired glycosidase, especially for hydrolysis of glycoconjugated precursors in musts and wines for the release of active aromatic compounds. A Shewanella sp. G5 strain was isolated from the intestinal content of benthonic organism (Munida subrrugosa) from different coastal areas of the Beagle Channel, Tierra del Fuego (Argentina). This marine bacterium was able to grow at a temperature range between 4 to 20 °C using different β ‐glycoside substrates, such as cellobiose, as carbon source. In this work, the Shewanella sp. G5 strain exhibited high β ‐glucosidase activity on plate at low temperature (4 and 20 °C). Two genes encoding different cold‐active β ‐glucosidases were amplified and sequenced and the nucleotide sequences were submitted to the GenBank. 16S rDNA and gyrB gene sequences were used for the molecular characterization of Shewanella sp. G5.Fil: Cristobal, Hector Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Breccia, Javier Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Abate, Carlos Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaWiley VCH Verlag2008-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/43472Cristobal, Hector Antonio; Breccia, Javier Dario; Abate, Carlos Mauricio; Isolation and molecular characterization of Shewanella sp. G5, a producer of cold-active β -D-glucosidases; Wiley VCH Verlag; Journal of Basic Microbiology; 48; 1; 12-2008; 16-240233-111XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/jobm.200700146info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jobm.200700146info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:43Zoai:ri.conicet.gov.ar:11336/43472instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:44.107CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Isolation and molecular characterization of Shewanella sp. G5, a producer of cold-active β -D-glucosidases
title Isolation and molecular characterization of Shewanella sp. G5, a producer of cold-active β -D-glucosidases
spellingShingle Isolation and molecular characterization of Shewanella sp. G5, a producer of cold-active β -D-glucosidases
Cristobal, Hector Antonio
Psychrotolerant
Â-Glucosidase
Gyrb
16s Rdna
title_short Isolation and molecular characterization of Shewanella sp. G5, a producer of cold-active β -D-glucosidases
title_full Isolation and molecular characterization of Shewanella sp. G5, a producer of cold-active β -D-glucosidases
title_fullStr Isolation and molecular characterization of Shewanella sp. G5, a producer of cold-active β -D-glucosidases
title_full_unstemmed Isolation and molecular characterization of Shewanella sp. G5, a producer of cold-active β -D-glucosidases
title_sort Isolation and molecular characterization of Shewanella sp. G5, a producer of cold-active β -D-glucosidases
dc.creator.none.fl_str_mv Cristobal, Hector Antonio
Breccia, Javier Dario
Abate, Carlos Mauricio
author Cristobal, Hector Antonio
author_facet Cristobal, Hector Antonio
Breccia, Javier Dario
Abate, Carlos Mauricio
author_role author
author2 Breccia, Javier Dario
Abate, Carlos Mauricio
author2_role author
author
dc.subject.none.fl_str_mv Psychrotolerant
Â-Glucosidase
Gyrb
16s Rdna
topic Psychrotolerant
Â-Glucosidase
Gyrb
16s Rdna
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv β ‐Glucosidase is a highly desired glycosidase, especially for hydrolysis of glycoconjugated precursors in musts and wines for the release of active aromatic compounds. A Shewanella sp. G5 strain was isolated from the intestinal content of benthonic organism (Munida subrrugosa) from different coastal areas of the Beagle Channel, Tierra del Fuego (Argentina). This marine bacterium was able to grow at a temperature range between 4 to 20 °C using different β ‐glycoside substrates, such as cellobiose, as carbon source. In this work, the Shewanella sp. G5 strain exhibited high β ‐glucosidase activity on plate at low temperature (4 and 20 °C). Two genes encoding different cold‐active β ‐glucosidases were amplified and sequenced and the nucleotide sequences were submitted to the GenBank. 16S rDNA and gyrB gene sequences were used for the molecular characterization of Shewanella sp. G5.
Fil: Cristobal, Hector Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Breccia, Javier Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Abate, Carlos Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
description β ‐Glucosidase is a highly desired glycosidase, especially for hydrolysis of glycoconjugated precursors in musts and wines for the release of active aromatic compounds. A Shewanella sp. G5 strain was isolated from the intestinal content of benthonic organism (Munida subrrugosa) from different coastal areas of the Beagle Channel, Tierra del Fuego (Argentina). This marine bacterium was able to grow at a temperature range between 4 to 20 °C using different β ‐glycoside substrates, such as cellobiose, as carbon source. In this work, the Shewanella sp. G5 strain exhibited high β ‐glucosidase activity on plate at low temperature (4 and 20 °C). Two genes encoding different cold‐active β ‐glucosidases were amplified and sequenced and the nucleotide sequences were submitted to the GenBank. 16S rDNA and gyrB gene sequences were used for the molecular characterization of Shewanella sp. G5.
publishDate 2008
dc.date.none.fl_str_mv 2008-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/43472
Cristobal, Hector Antonio; Breccia, Javier Dario; Abate, Carlos Mauricio; Isolation and molecular characterization of Shewanella sp. G5, a producer of cold-active β -D-glucosidases; Wiley VCH Verlag; Journal of Basic Microbiology; 48; 1; 12-2008; 16-24
0233-111X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/43472
identifier_str_mv Cristobal, Hector Antonio; Breccia, Javier Dario; Abate, Carlos Mauricio; Isolation and molecular characterization of Shewanella sp. G5, a producer of cold-active β -D-glucosidases; Wiley VCH Verlag; Journal of Basic Microbiology; 48; 1; 12-2008; 16-24
0233-111X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/jobm.200700146
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jobm.200700146
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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