Role of the quorum sensing mechanism in the response of pseudomonas aeruginosa to lethal and sublethal UVA irradiation

Autores
Costa, Cristina Susana; Pezzoni, Magdalena; Fernández, Rubén O.; Pizarro, Ramón Augusto
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The role of quorum sensing (QS) in the response of Pseudomonas aeruginosa to UVA radiation was investigated in the PAO1 strain and derivatives defective in the synthesis of the QS signals 3OC12-HSL (lasI strain), C4-HSL (rhlI strain) or both (lasI rhlI strain). Cell viability measurements demonstrated that the double mutant was significantly more sensitive to UVA than single mutants, which in turn showed reduced cell survival with regard to the PAO1 strain. Irradiation under nitrogen atmosphere and chemiluminescence measurements indicated the oxidative nature of the UVA-induced damage. The activity of the antioxidant enzymes catalase and superoxide dismutase was assayed in these strains before and after irradiation, and a strong correlation between catalase levels and UVA sensitivity was observed. When a sublethal UVA dose was applied to PAO1, a growth delay was observed and this mechanism depended on the rhl system. Moreover, low doses of UVA irradiation triplicated the level of C4-HSL in log PAO1 cells. It is concluded that QS is fundamental in the defense against the toxic effects of UVA in P. aeruginosa. The induction of the QS system by UVA independently of cell density could function as an adaptative strategy to withstand this hostile environmental condition.
Fil: Costa, Cristina Susana. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); Argentina
Fil: Pezzoni, Magdalena. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernández, Rubén O.. Comisión Nacional de Energía Atómica; Argentina
Fil: Pizarro, Ramón Augusto. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); Argentina
Materia
PSEUDOMONAS AERUGINOSA
UVA IRRADIATION
QUORUM SENSING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/187291
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/187291
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Role of the quorum sensing mechanism in the response of pseudomonas aeruginosa to lethal and sublethal UVA irradiationCosta, Cristina SusanaPezzoni, MagdalenaFernández, Rubén O.Pizarro, Ramón AugustoPSEUDOMONAS AERUGINOSAUVA IRRADIATIONQUORUM SENSINGhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The role of quorum sensing (QS) in the response of Pseudomonas aeruginosa to UVA radiation was investigated in the PAO1 strain and derivatives defective in the synthesis of the QS signals 3OC12-HSL (lasI strain), C4-HSL (rhlI strain) or both (lasI rhlI strain). Cell viability measurements demonstrated that the double mutant was significantly more sensitive to UVA than single mutants, which in turn showed reduced cell survival with regard to the PAO1 strain. Irradiation under nitrogen atmosphere and chemiluminescence measurements indicated the oxidative nature of the UVA-induced damage. The activity of the antioxidant enzymes catalase and superoxide dismutase was assayed in these strains before and after irradiation, and a strong correlation between catalase levels and UVA sensitivity was observed. When a sublethal UVA dose was applied to PAO1, a growth delay was observed and this mechanism depended on the rhl system. Moreover, low doses of UVA irradiation triplicated the level of C4-HSL in log PAO1 cells. It is concluded that QS is fundamental in the defense against the toxic effects of UVA in P. aeruginosa. The induction of the QS system by UVA independently of cell density could function as an adaptative strategy to withstand this hostile environmental condition.Fil: Costa, Cristina Susana. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); ArgentinaFil: Pezzoni, Magdalena. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fernández, Rubén O.. Comisión Nacional de Energía Atómica; ArgentinaFil: Pizarro, Ramón Augusto. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); ArgentinaWiley Blackwell Publishing, Inc2010-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/187291Costa, Cristina Susana; Pezzoni, Magdalena; Fernández, Rubén O.; Pizarro, Ramón Augusto; Role of the quorum sensing mechanism in the response of pseudomonas aeruginosa to lethal and sublethal UVA irradiation; Wiley Blackwell Publishing, Inc; Photochemistry and Photobiology; 86; 6; 11-2010; 1334-13420031-8655CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/j.1751-1097.2010.00800.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1751-1097.2010.00800.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:04:50Zoai:ri.conicet.gov.ar:11336/187291instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:04:50.829CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Role of the quorum sensing mechanism in the response of pseudomonas aeruginosa to lethal and sublethal UVA irradiation
title Role of the quorum sensing mechanism in the response of pseudomonas aeruginosa to lethal and sublethal UVA irradiation
spellingShingle Role of the quorum sensing mechanism in the response of pseudomonas aeruginosa to lethal and sublethal UVA irradiation
Costa, Cristina Susana
PSEUDOMONAS AERUGINOSA
UVA IRRADIATION
QUORUM SENSING
title_short Role of the quorum sensing mechanism in the response of pseudomonas aeruginosa to lethal and sublethal UVA irradiation
title_full Role of the quorum sensing mechanism in the response of pseudomonas aeruginosa to lethal and sublethal UVA irradiation
title_fullStr Role of the quorum sensing mechanism in the response of pseudomonas aeruginosa to lethal and sublethal UVA irradiation
title_full_unstemmed Role of the quorum sensing mechanism in the response of pseudomonas aeruginosa to lethal and sublethal UVA irradiation
title_sort Role of the quorum sensing mechanism in the response of pseudomonas aeruginosa to lethal and sublethal UVA irradiation
dc.creator.none.fl_str_mv Costa, Cristina Susana
Pezzoni, Magdalena
Fernández, Rubén O.
Pizarro, Ramón Augusto
author Costa, Cristina Susana
author_facet Costa, Cristina Susana
Pezzoni, Magdalena
Fernández, Rubén O.
Pizarro, Ramón Augusto
author_role author
author2 Pezzoni, Magdalena
Fernández, Rubén O.
Pizarro, Ramón Augusto
author2_role author
author
author
dc.subject.none.fl_str_mv PSEUDOMONAS AERUGINOSA
UVA IRRADIATION
QUORUM SENSING
topic PSEUDOMONAS AERUGINOSA
UVA IRRADIATION
QUORUM SENSING
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The role of quorum sensing (QS) in the response of Pseudomonas aeruginosa to UVA radiation was investigated in the PAO1 strain and derivatives defective in the synthesis of the QS signals 3OC12-HSL (lasI strain), C4-HSL (rhlI strain) or both (lasI rhlI strain). Cell viability measurements demonstrated that the double mutant was significantly more sensitive to UVA than single mutants, which in turn showed reduced cell survival with regard to the PAO1 strain. Irradiation under nitrogen atmosphere and chemiluminescence measurements indicated the oxidative nature of the UVA-induced damage. The activity of the antioxidant enzymes catalase and superoxide dismutase was assayed in these strains before and after irradiation, and a strong correlation between catalase levels and UVA sensitivity was observed. When a sublethal UVA dose was applied to PAO1, a growth delay was observed and this mechanism depended on the rhl system. Moreover, low doses of UVA irradiation triplicated the level of C4-HSL in log PAO1 cells. It is concluded that QS is fundamental in the defense against the toxic effects of UVA in P. aeruginosa. The induction of the QS system by UVA independently of cell density could function as an adaptative strategy to withstand this hostile environmental condition.
Fil: Costa, Cristina Susana. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); Argentina
Fil: Pezzoni, Magdalena. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernández, Rubén O.. Comisión Nacional de Energía Atómica; Argentina
Fil: Pizarro, Ramón Augusto. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); Argentina
description The role of quorum sensing (QS) in the response of Pseudomonas aeruginosa to UVA radiation was investigated in the PAO1 strain and derivatives defective in the synthesis of the QS signals 3OC12-HSL (lasI strain), C4-HSL (rhlI strain) or both (lasI rhlI strain). Cell viability measurements demonstrated that the double mutant was significantly more sensitive to UVA than single mutants, which in turn showed reduced cell survival with regard to the PAO1 strain. Irradiation under nitrogen atmosphere and chemiluminescence measurements indicated the oxidative nature of the UVA-induced damage. The activity of the antioxidant enzymes catalase and superoxide dismutase was assayed in these strains before and after irradiation, and a strong correlation between catalase levels and UVA sensitivity was observed. When a sublethal UVA dose was applied to PAO1, a growth delay was observed and this mechanism depended on the rhl system. Moreover, low doses of UVA irradiation triplicated the level of C4-HSL in log PAO1 cells. It is concluded that QS is fundamental in the defense against the toxic effects of UVA in P. aeruginosa. The induction of the QS system by UVA independently of cell density could function as an adaptative strategy to withstand this hostile environmental condition.
publishDate 2010
dc.date.none.fl_str_mv 2010-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/187291
Costa, Cristina Susana; Pezzoni, Magdalena; Fernández, Rubén O.; Pizarro, Ramón Augusto; Role of the quorum sensing mechanism in the response of pseudomonas aeruginosa to lethal and sublethal UVA irradiation; Wiley Blackwell Publishing, Inc; Photochemistry and Photobiology; 86; 6; 11-2010; 1334-1342
0031-8655
CONICET Digital
CONICET
url http://hdl.handle.net/11336/187291
identifier_str_mv Costa, Cristina Susana; Pezzoni, Magdalena; Fernández, Rubén O.; Pizarro, Ramón Augusto; Role of the quorum sensing mechanism in the response of pseudomonas aeruginosa to lethal and sublethal UVA irradiation; Wiley Blackwell Publishing, Inc; Photochemistry and Photobiology; 86; 6; 11-2010; 1334-1342
0031-8655
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/j.1751-1097.2010.00800.x
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1751-1097.2010.00800.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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