StRemorin1.3 hampers Potato virus X TGBp1 ability to increase plasmodesmata permeability, but does not interfere with its silencing suppressor activity

Autores
Perraki, Artemis; Binaghi, Maria; Mecchia, Martin Alejandro; Gronnier, Julien; German Retana, Sylvie; Mongrand, Sébastien; Bayer, Emmanuelle; Zelada, Alicia Mercedes; Germain, Véronique
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
TThe Triple Gene Block 1 (TGBp1) protein encoded by the Potato virus X is a multifunctional protein that acts as a suppressor of RNA silencing or facilitates the passage of virus from cell to cell by promoting the plasmodesmata opening. We previously showed that the membrane raft protein StRemorin1.3 is able to impair PVX infection. Here, we show that overexpressed StRemorin1.3 does not impair the silencing suppressor activity of TGBp1, but affects its ability to increase plasmodesmata permeability. A similar effect on plasmodesmata permeability was observed with other movement proteins, suggesting that REM is a general regulator of plasmodesmal size exclusion limit. These results add to our knowledge of the mechanisms underlying the StREM1.3 role in virus infection.
Fil: Perraki, Artemis. Universite de Bordeaux; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Binaghi, Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Fil: Mecchia, Martin Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Fil: Gronnier, Julien. Universite de Bordeaux; Francia. Centre National de la Recherche Scientifique; Francia
Fil: German Retana, Sylvie. Institut National de la Recherche Agronomique; Francia
Fil: Mongrand, Sébastien. Universite de Bordeaux; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Bayer, Emmanuelle. Universite de Bordeaux; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Zelada, Alicia Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Fil: Germain, Véronique. Centre National de la Recherche Scientifique; Francia. Universite de Bordeaux; Francia
Materia
Remorin
Tgbp1
Gating
Ptgs
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/32172
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/32172
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling StRemorin1.3 hampers Potato virus X TGBp1 ability to increase plasmodesmata permeability, but does not interfere with its silencing suppressor activityPerraki, ArtemisBinaghi, MariaMecchia, Martin AlejandroGronnier, JulienGerman Retana, SylvieMongrand, SébastienBayer, EmmanuelleZelada, Alicia MercedesGermain, VéroniqueRemorinTgbp1GatingPtgshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1TThe Triple Gene Block 1 (TGBp1) protein encoded by the Potato virus X is a multifunctional protein that acts as a suppressor of RNA silencing or facilitates the passage of virus from cell to cell by promoting the plasmodesmata opening. We previously showed that the membrane raft protein StRemorin1.3 is able to impair PVX infection. Here, we show that overexpressed StRemorin1.3 does not impair the silencing suppressor activity of TGBp1, but affects its ability to increase plasmodesmata permeability. A similar effect on plasmodesmata permeability was observed with other movement proteins, suggesting that REM is a general regulator of plasmodesmal size exclusion limit. These results add to our knowledge of the mechanisms underlying the StREM1.3 role in virus infection.Fil: Perraki, Artemis. Universite de Bordeaux; Francia. Centre National de la Recherche Scientifique; FranciaFil: Binaghi, Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; ArgentinaFil: Mecchia, Martin Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; ArgentinaFil: Gronnier, Julien. Universite de Bordeaux; Francia. Centre National de la Recherche Scientifique; FranciaFil: German Retana, Sylvie. Institut National de la Recherche Agronomique; FranciaFil: Mongrand, Sébastien. Universite de Bordeaux; Francia. Centre National de la Recherche Scientifique; FranciaFil: Bayer, Emmanuelle. Universite de Bordeaux; Francia. Centre National de la Recherche Scientifique; FranciaFil: Zelada, Alicia Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; ArgentinaFil: Germain, Véronique. Centre National de la Recherche Scientifique; Francia. Universite de Bordeaux; FranciaElsevier Science2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/32172Perraki, Artemis; Binaghi, Maria; Mecchia, Martin Alejandro; Gronnier, Julien; German Retana, Sylvie; et al.; StRemorin1.3 hampers Potato virus X TGBp1 ability to increase plasmodesmata permeability, but does not interfere with its silencing suppressor activity; Elsevier Science; FEBS Letters; 588; 9; 3-2014; 1699-17050014-5793CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.febslet.2014.03.014info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0014579314002191info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:34Zoai:ri.conicet.gov.ar:11336/32172instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:34.476CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv StRemorin1.3 hampers Potato virus X TGBp1 ability to increase plasmodesmata permeability, but does not interfere with its silencing suppressor activity
title StRemorin1.3 hampers Potato virus X TGBp1 ability to increase plasmodesmata permeability, but does not interfere with its silencing suppressor activity
spellingShingle StRemorin1.3 hampers Potato virus X TGBp1 ability to increase plasmodesmata permeability, but does not interfere with its silencing suppressor activity
Perraki, Artemis
Remorin
Tgbp1
Gating
Ptgs
title_short StRemorin1.3 hampers Potato virus X TGBp1 ability to increase plasmodesmata permeability, but does not interfere with its silencing suppressor activity
title_full StRemorin1.3 hampers Potato virus X TGBp1 ability to increase plasmodesmata permeability, but does not interfere with its silencing suppressor activity
title_fullStr StRemorin1.3 hampers Potato virus X TGBp1 ability to increase plasmodesmata permeability, but does not interfere with its silencing suppressor activity
title_full_unstemmed StRemorin1.3 hampers Potato virus X TGBp1 ability to increase plasmodesmata permeability, but does not interfere with its silencing suppressor activity
title_sort StRemorin1.3 hampers Potato virus X TGBp1 ability to increase plasmodesmata permeability, but does not interfere with its silencing suppressor activity
dc.creator.none.fl_str_mv Perraki, Artemis
Binaghi, Maria
Mecchia, Martin Alejandro
Gronnier, Julien
German Retana, Sylvie
Mongrand, Sébastien
Bayer, Emmanuelle
Zelada, Alicia Mercedes
Germain, Véronique
author Perraki, Artemis
author_facet Perraki, Artemis
Binaghi, Maria
Mecchia, Martin Alejandro
Gronnier, Julien
German Retana, Sylvie
Mongrand, Sébastien
Bayer, Emmanuelle
Zelada, Alicia Mercedes
Germain, Véronique
author_role author
author2 Binaghi, Maria
Mecchia, Martin Alejandro
Gronnier, Julien
German Retana, Sylvie
Mongrand, Sébastien
Bayer, Emmanuelle
Zelada, Alicia Mercedes
Germain, Véronique
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Remorin
Tgbp1
Gating
Ptgs
topic Remorin
Tgbp1
Gating
Ptgs
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv TThe Triple Gene Block 1 (TGBp1) protein encoded by the Potato virus X is a multifunctional protein that acts as a suppressor of RNA silencing or facilitates the passage of virus from cell to cell by promoting the plasmodesmata opening. We previously showed that the membrane raft protein StRemorin1.3 is able to impair PVX infection. Here, we show that overexpressed StRemorin1.3 does not impair the silencing suppressor activity of TGBp1, but affects its ability to increase plasmodesmata permeability. A similar effect on plasmodesmata permeability was observed with other movement proteins, suggesting that REM is a general regulator of plasmodesmal size exclusion limit. These results add to our knowledge of the mechanisms underlying the StREM1.3 role in virus infection.
Fil: Perraki, Artemis. Universite de Bordeaux; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Binaghi, Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Fil: Mecchia, Martin Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Fil: Gronnier, Julien. Universite de Bordeaux; Francia. Centre National de la Recherche Scientifique; Francia
Fil: German Retana, Sylvie. Institut National de la Recherche Agronomique; Francia
Fil: Mongrand, Sébastien. Universite de Bordeaux; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Bayer, Emmanuelle. Universite de Bordeaux; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Zelada, Alicia Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Fil: Germain, Véronique. Centre National de la Recherche Scientifique; Francia. Universite de Bordeaux; Francia
description TThe Triple Gene Block 1 (TGBp1) protein encoded by the Potato virus X is a multifunctional protein that acts as a suppressor of RNA silencing or facilitates the passage of virus from cell to cell by promoting the plasmodesmata opening. We previously showed that the membrane raft protein StRemorin1.3 is able to impair PVX infection. Here, we show that overexpressed StRemorin1.3 does not impair the silencing suppressor activity of TGBp1, but affects its ability to increase plasmodesmata permeability. A similar effect on plasmodesmata permeability was observed with other movement proteins, suggesting that REM is a general regulator of plasmodesmal size exclusion limit. These results add to our knowledge of the mechanisms underlying the StREM1.3 role in virus infection.
publishDate 2014
dc.date.none.fl_str_mv 2014-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/32172
Perraki, Artemis; Binaghi, Maria; Mecchia, Martin Alejandro; Gronnier, Julien; German Retana, Sylvie; et al.; StRemorin1.3 hampers Potato virus X TGBp1 ability to increase plasmodesmata permeability, but does not interfere with its silencing suppressor activity; Elsevier Science; FEBS Letters; 588; 9; 3-2014; 1699-1705
0014-5793
CONICET Digital
CONICET
url http://hdl.handle.net/11336/32172
identifier_str_mv Perraki, Artemis; Binaghi, Maria; Mecchia, Martin Alejandro; Gronnier, Julien; German Retana, Sylvie; et al.; StRemorin1.3 hampers Potato virus X TGBp1 ability to increase plasmodesmata permeability, but does not interfere with its silencing suppressor activity; Elsevier Science; FEBS Letters; 588; 9; 3-2014; 1699-1705
0014-5793
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.febslet.2014.03.014
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0014579314002191
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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