On the diversity of F420-dependent oxidoreductases: A sequence- and structure-based classification

Autores
Mascotti, María Laura; Juri Ayub, Maximiliano; Fraaije, Marco Wilhelmus
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The F420 deazaflavin cofactor is an intriguing molecule as it structurally resembles the canonical flavin cofactor, although behaves as a nicotinamide cofactor due to its obligate hydride-transfer reactivity and similar low redox potential. Since its discovery, numerous enzymes relying on it have been described. The known deazaflavoproteins are taxonomically restricted to Archaea and Bacteria. The biochemistry of the deazaflavoenzymes is diverse and they exhibit great structural variability. In this study a thorough sequence and structural homology evolutionary analysis was performed in order to generate an overarching classification of the F420-dependent oxidoreductases. Five different deazaflavoenzyme Classes (I–V) are described according to their structural folds as follows: Class I encompassing the TIM-barrel F420-dependent enzymes; Class II including the Rossmann fold F420-dependent enzymes; Class III comprising the β-roll F420-dependent enzymes; Class IV which exclusively gathers the SH3 barrel F420-dependent enzymes and Class V including the three layer ββα sandwich F420-dependent enzymes. This classification provides a framework for the identification and biochemical characterization of novel deazaflavoenzymes.
Fil: Mascotti, María Laura. University of Groningen; Países Bajos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Juri Ayub, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Fraaije, Marco Wilhelmus. University of Groningen; Países Bajos
Materia
DEAZAFLAVOENZYMES
EVOLUTIONARY CLASSIFICATION
F420-DEPENDENT OXIDOREDUCTASES
MOLECULAR EVOLUTION
STRUCTURAL CLASSIFICATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/181662
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/181662
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling On the diversity of F420-dependent oxidoreductases: A sequence- and structure-based classificationMascotti, María LauraJuri Ayub, MaximilianoFraaije, Marco WilhelmusDEAZAFLAVOENZYMESEVOLUTIONARY CLASSIFICATIONF420-DEPENDENT OXIDOREDUCTASESMOLECULAR EVOLUTIONSTRUCTURAL CLASSIFICATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.2https://purl.org/becyt/ford/1The F420 deazaflavin cofactor is an intriguing molecule as it structurally resembles the canonical flavin cofactor, although behaves as a nicotinamide cofactor due to its obligate hydride-transfer reactivity and similar low redox potential. Since its discovery, numerous enzymes relying on it have been described. The known deazaflavoproteins are taxonomically restricted to Archaea and Bacteria. The biochemistry of the deazaflavoenzymes is diverse and they exhibit great structural variability. In this study a thorough sequence and structural homology evolutionary analysis was performed in order to generate an overarching classification of the F420-dependent oxidoreductases. Five different deazaflavoenzyme Classes (I–V) are described according to their structural folds as follows: Class I encompassing the TIM-barrel F420-dependent enzymes; Class II including the Rossmann fold F420-dependent enzymes; Class III comprising the β-roll F420-dependent enzymes; Class IV which exclusively gathers the SH3 barrel F420-dependent enzymes and Class V including the three layer ββα sandwich F420-dependent enzymes. This classification provides a framework for the identification and biochemical characterization of novel deazaflavoenzymes.Fil: Mascotti, María Laura. University of Groningen; Países Bajos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Juri Ayub, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Fraaije, Marco Wilhelmus. University of Groningen; Países BajosWiley-liss, div John Wiley & Sons Inc.2021-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/181662Mascotti, María Laura; Juri Ayub, Maximiliano; Fraaije, Marco Wilhelmus; On the diversity of F420-dependent oxidoreductases: A sequence- and structure-based classification; Wiley-liss, div John Wiley & Sons Inc.; Proteins: Structure, Function And Genetics; 89; 11; 6-2021; 1497-15070887-3585CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/prot.26170info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/prot.26170info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:57:42Zoai:ri.conicet.gov.ar:11336/181662instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:57:42.774CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv On the diversity of F420-dependent oxidoreductases: A sequence- and structure-based classification
title On the diversity of F420-dependent oxidoreductases: A sequence- and structure-based classification
spellingShingle On the diversity of F420-dependent oxidoreductases: A sequence- and structure-based classification
Mascotti, María Laura
DEAZAFLAVOENZYMES
EVOLUTIONARY CLASSIFICATION
F420-DEPENDENT OXIDOREDUCTASES
MOLECULAR EVOLUTION
STRUCTURAL CLASSIFICATION
title_short On the diversity of F420-dependent oxidoreductases: A sequence- and structure-based classification
title_full On the diversity of F420-dependent oxidoreductases: A sequence- and structure-based classification
title_fullStr On the diversity of F420-dependent oxidoreductases: A sequence- and structure-based classification
title_full_unstemmed On the diversity of F420-dependent oxidoreductases: A sequence- and structure-based classification
title_sort On the diversity of F420-dependent oxidoreductases: A sequence- and structure-based classification
dc.creator.none.fl_str_mv Mascotti, María Laura
Juri Ayub, Maximiliano
Fraaije, Marco Wilhelmus
author Mascotti, María Laura
author_facet Mascotti, María Laura
Juri Ayub, Maximiliano
Fraaije, Marco Wilhelmus
author_role author
author2 Juri Ayub, Maximiliano
Fraaije, Marco Wilhelmus
author2_role author
author
dc.subject.none.fl_str_mv DEAZAFLAVOENZYMES
EVOLUTIONARY CLASSIFICATION
F420-DEPENDENT OXIDOREDUCTASES
MOLECULAR EVOLUTION
STRUCTURAL CLASSIFICATION
topic DEAZAFLAVOENZYMES
EVOLUTIONARY CLASSIFICATION
F420-DEPENDENT OXIDOREDUCTASES
MOLECULAR EVOLUTION
STRUCTURAL CLASSIFICATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.2
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The F420 deazaflavin cofactor is an intriguing molecule as it structurally resembles the canonical flavin cofactor, although behaves as a nicotinamide cofactor due to its obligate hydride-transfer reactivity and similar low redox potential. Since its discovery, numerous enzymes relying on it have been described. The known deazaflavoproteins are taxonomically restricted to Archaea and Bacteria. The biochemistry of the deazaflavoenzymes is diverse and they exhibit great structural variability. In this study a thorough sequence and structural homology evolutionary analysis was performed in order to generate an overarching classification of the F420-dependent oxidoreductases. Five different deazaflavoenzyme Classes (I–V) are described according to their structural folds as follows: Class I encompassing the TIM-barrel F420-dependent enzymes; Class II including the Rossmann fold F420-dependent enzymes; Class III comprising the β-roll F420-dependent enzymes; Class IV which exclusively gathers the SH3 barrel F420-dependent enzymes and Class V including the three layer ββα sandwich F420-dependent enzymes. This classification provides a framework for the identification and biochemical characterization of novel deazaflavoenzymes.
Fil: Mascotti, María Laura. University of Groningen; Países Bajos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Juri Ayub, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Fraaije, Marco Wilhelmus. University of Groningen; Países Bajos
description The F420 deazaflavin cofactor is an intriguing molecule as it structurally resembles the canonical flavin cofactor, although behaves as a nicotinamide cofactor due to its obligate hydride-transfer reactivity and similar low redox potential. Since its discovery, numerous enzymes relying on it have been described. The known deazaflavoproteins are taxonomically restricted to Archaea and Bacteria. The biochemistry of the deazaflavoenzymes is diverse and they exhibit great structural variability. In this study a thorough sequence and structural homology evolutionary analysis was performed in order to generate an overarching classification of the F420-dependent oxidoreductases. Five different deazaflavoenzyme Classes (I–V) are described according to their structural folds as follows: Class I encompassing the TIM-barrel F420-dependent enzymes; Class II including the Rossmann fold F420-dependent enzymes; Class III comprising the β-roll F420-dependent enzymes; Class IV which exclusively gathers the SH3 barrel F420-dependent enzymes and Class V including the three layer ββα sandwich F420-dependent enzymes. This classification provides a framework for the identification and biochemical characterization of novel deazaflavoenzymes.
publishDate 2021
dc.date.none.fl_str_mv 2021-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/181662
Mascotti, María Laura; Juri Ayub, Maximiliano; Fraaije, Marco Wilhelmus; On the diversity of F420-dependent oxidoreductases: A sequence- and structure-based classification; Wiley-liss, div John Wiley & Sons Inc.; Proteins: Structure, Function And Genetics; 89; 11; 6-2021; 1497-1507
0887-3585
CONICET Digital
CONICET
url http://hdl.handle.net/11336/181662
identifier_str_mv Mascotti, María Laura; Juri Ayub, Maximiliano; Fraaije, Marco Wilhelmus; On the diversity of F420-dependent oxidoreductases: A sequence- and structure-based classification; Wiley-liss, div John Wiley & Sons Inc.; Proteins: Structure, Function And Genetics; 89; 11; 6-2021; 1497-1507
0887-3585
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/prot.26170
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/prot.26170
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, div John Wiley & Sons Inc.
publisher.none.fl_str_mv Wiley-liss, div John Wiley & Sons Inc.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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