Label-free graphene oxide-based surface plasmon resonance immunosensor for the quantification of Galectin-3, a novel cardiac biomarker

Autores
Primo, Emiliano Nicolás; Kogan, Marcelo Javier; Verdejo, Hugo E.; Bollo, Soledad; Rubianes, María Dolores; Rivas, Gustavo Adolfo
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We report the first optical biosensor for the novel and important cardiac biomarker, galectin-3 (Gal3), using the anti-Gal3 antibody as a biorecognition element and surface plasmon resonance (SPR) for transducing the bioaffinity event. The immunosensing platform was built at a thiolated Au surface modified by self-assembling four bilayers of poly(diallyldimethylammonium chloride) and graphene oxide (GO), followed by the covalent attachment of 3-aminephenylboronic acid (3ABA). The importance of GO, both as the anchoring point of the antibody and as a field enhancer for improving the biosensor sensitivity, was critically discussed. The advantages of using 3ABA to orientate the anti-Gal3 antibody through the selective link to the Fc region were also demonstrated. The new platform represents an interesting alternative for the label-free biosensing of Gal3 in the whole range of clinically relevant concentrations (linear range between 10.0 and 50.0 ng mL-1, detection limit of 2.0 ng mL-1) with successful application for Gal3 biosensing in enriched human serum samples.
Fil: Primo, Emiliano Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina
Fil: Kogan, Marcelo Javier. Universidad de Chile; Chile
Fil: Verdejo, Hugo E.. Universidad de Chile; Chile. Pontificia Universidad Catolica de Chile. Facultad de Ciencias Biológicas; Chile
Fil: Bollo, Soledad. Pontificia Universidad Católica de Chile; Chile
Fil: Rubianes, María Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Materia
CARDIAC BIOMARKER
GALECTIN3
GRAPHENE OXIDE
IMMUNOSENSOR
LAYER-BY-LAYER SELF-ASSEMBLY
SPR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/85208
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/85208
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Label-free graphene oxide-based surface plasmon resonance immunosensor for the quantification of Galectin-3, a novel cardiac biomarkerPrimo, Emiliano NicolásKogan, Marcelo JavierVerdejo, Hugo E.Bollo, SoledadRubianes, María DoloresRivas, Gustavo AdolfoCARDIAC BIOMARKERGALECTIN3GRAPHENE OXIDEIMMUNOSENSORLAYER-BY-LAYER SELF-ASSEMBLYSPRhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1We report the first optical biosensor for the novel and important cardiac biomarker, galectin-3 (Gal3), using the anti-Gal3 antibody as a biorecognition element and surface plasmon resonance (SPR) for transducing the bioaffinity event. The immunosensing platform was built at a thiolated Au surface modified by self-assembling four bilayers of poly(diallyldimethylammonium chloride) and graphene oxide (GO), followed by the covalent attachment of 3-aminephenylboronic acid (3ABA). The importance of GO, both as the anchoring point of the antibody and as a field enhancer for improving the biosensor sensitivity, was critically discussed. The advantages of using 3ABA to orientate the anti-Gal3 antibody through the selective link to the Fc region were also demonstrated. The new platform represents an interesting alternative for the label-free biosensing of Gal3 in the whole range of clinically relevant concentrations (linear range between 10.0 and 50.0 ng mL-1, detection limit of 2.0 ng mL-1) with successful application for Gal3 biosensing in enriched human serum samples.Fil: Primo, Emiliano Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; ArgentinaFil: Kogan, Marcelo Javier. Universidad de Chile; ChileFil: Verdejo, Hugo E.. Universidad de Chile; Chile. Pontificia Universidad Catolica de Chile. Facultad de Ciencias Biológicas; ChileFil: Bollo, Soledad. Pontificia Universidad Católica de Chile; ChileFil: Rubianes, María Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaAmerican Chemical Society2018-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/85208Primo, Emiliano Nicolás; Kogan, Marcelo Javier; Verdejo, Hugo E.; Bollo, Soledad; Rubianes, María Dolores; et al.; Label-free graphene oxide-based surface plasmon resonance immunosensor for the quantification of Galectin-3, a novel cardiac biomarker; American Chemical Society; ACS Applied Materials & Interfaces; 10; 28; 7-2018; 23501-235081944-82441944-8252CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1021/acsami.8b03039info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/acsami.8b03039info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:37:17Zoai:ri.conicet.gov.ar:11336/85208instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:37:18.004CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Label-free graphene oxide-based surface plasmon resonance immunosensor for the quantification of Galectin-3, a novel cardiac biomarker
title Label-free graphene oxide-based surface plasmon resonance immunosensor for the quantification of Galectin-3, a novel cardiac biomarker
spellingShingle Label-free graphene oxide-based surface plasmon resonance immunosensor for the quantification of Galectin-3, a novel cardiac biomarker
Primo, Emiliano Nicolás
CARDIAC BIOMARKER
GALECTIN3
GRAPHENE OXIDE
IMMUNOSENSOR
LAYER-BY-LAYER SELF-ASSEMBLY
SPR
title_short Label-free graphene oxide-based surface plasmon resonance immunosensor for the quantification of Galectin-3, a novel cardiac biomarker
title_full Label-free graphene oxide-based surface plasmon resonance immunosensor for the quantification of Galectin-3, a novel cardiac biomarker
title_fullStr Label-free graphene oxide-based surface plasmon resonance immunosensor for the quantification of Galectin-3, a novel cardiac biomarker
title_full_unstemmed Label-free graphene oxide-based surface plasmon resonance immunosensor for the quantification of Galectin-3, a novel cardiac biomarker
title_sort Label-free graphene oxide-based surface plasmon resonance immunosensor for the quantification of Galectin-3, a novel cardiac biomarker
dc.creator.none.fl_str_mv Primo, Emiliano Nicolás
Kogan, Marcelo Javier
Verdejo, Hugo E.
Bollo, Soledad
Rubianes, María Dolores
Rivas, Gustavo Adolfo
author Primo, Emiliano Nicolás
author_facet Primo, Emiliano Nicolás
Kogan, Marcelo Javier
Verdejo, Hugo E.
Bollo, Soledad
Rubianes, María Dolores
Rivas, Gustavo Adolfo
author_role author
author2 Kogan, Marcelo Javier
Verdejo, Hugo E.
Bollo, Soledad
Rubianes, María Dolores
Rivas, Gustavo Adolfo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CARDIAC BIOMARKER
GALECTIN3
GRAPHENE OXIDE
IMMUNOSENSOR
LAYER-BY-LAYER SELF-ASSEMBLY
SPR
topic CARDIAC BIOMARKER
GALECTIN3
GRAPHENE OXIDE
IMMUNOSENSOR
LAYER-BY-LAYER SELF-ASSEMBLY
SPR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We report the first optical biosensor for the novel and important cardiac biomarker, galectin-3 (Gal3), using the anti-Gal3 antibody as a biorecognition element and surface plasmon resonance (SPR) for transducing the bioaffinity event. The immunosensing platform was built at a thiolated Au surface modified by self-assembling four bilayers of poly(diallyldimethylammonium chloride) and graphene oxide (GO), followed by the covalent attachment of 3-aminephenylboronic acid (3ABA). The importance of GO, both as the anchoring point of the antibody and as a field enhancer for improving the biosensor sensitivity, was critically discussed. The advantages of using 3ABA to orientate the anti-Gal3 antibody through the selective link to the Fc region were also demonstrated. The new platform represents an interesting alternative for the label-free biosensing of Gal3 in the whole range of clinically relevant concentrations (linear range between 10.0 and 50.0 ng mL-1, detection limit of 2.0 ng mL-1) with successful application for Gal3 biosensing in enriched human serum samples.
Fil: Primo, Emiliano Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina
Fil: Kogan, Marcelo Javier. Universidad de Chile; Chile
Fil: Verdejo, Hugo E.. Universidad de Chile; Chile. Pontificia Universidad Catolica de Chile. Facultad de Ciencias Biológicas; Chile
Fil: Bollo, Soledad. Pontificia Universidad Católica de Chile; Chile
Fil: Rubianes, María Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
description We report the first optical biosensor for the novel and important cardiac biomarker, galectin-3 (Gal3), using the anti-Gal3 antibody as a biorecognition element and surface plasmon resonance (SPR) for transducing the bioaffinity event. The immunosensing platform was built at a thiolated Au surface modified by self-assembling four bilayers of poly(diallyldimethylammonium chloride) and graphene oxide (GO), followed by the covalent attachment of 3-aminephenylboronic acid (3ABA). The importance of GO, both as the anchoring point of the antibody and as a field enhancer for improving the biosensor sensitivity, was critically discussed. The advantages of using 3ABA to orientate the anti-Gal3 antibody through the selective link to the Fc region were also demonstrated. The new platform represents an interesting alternative for the label-free biosensing of Gal3 in the whole range of clinically relevant concentrations (linear range between 10.0 and 50.0 ng mL-1, detection limit of 2.0 ng mL-1) with successful application for Gal3 biosensing in enriched human serum samples.
publishDate 2018
dc.date.none.fl_str_mv 2018-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/85208
Primo, Emiliano Nicolás; Kogan, Marcelo Javier; Verdejo, Hugo E.; Bollo, Soledad; Rubianes, María Dolores; et al.; Label-free graphene oxide-based surface plasmon resonance immunosensor for the quantification of Galectin-3, a novel cardiac biomarker; American Chemical Society; ACS Applied Materials & Interfaces; 10; 28; 7-2018; 23501-23508
1944-8244
1944-8252
CONICET Digital
CONICET
url http://hdl.handle.net/11336/85208
identifier_str_mv Primo, Emiliano Nicolás; Kogan, Marcelo Javier; Verdejo, Hugo E.; Bollo, Soledad; Rubianes, María Dolores; et al.; Label-free graphene oxide-based surface plasmon resonance immunosensor for the quantification of Galectin-3, a novel cardiac biomarker; American Chemical Society; ACS Applied Materials & Interfaces; 10; 28; 7-2018; 23501-23508
1944-8244
1944-8252
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1021/acsami.8b03039
info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/acsami.8b03039
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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