Label-free graphene oxide-based surface plasmon resonance immunosensor for the quantification of Galectin-3, a novel cardiac biomarker
- Autores
- Primo, Emiliano Nicolás; Kogan, Marcelo Javier; Verdejo, Hugo E.; Bollo, Soledad; Rubianes, María Dolores; Rivas, Gustavo Adolfo
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- We report the first optical biosensor for the novel and important cardiac biomarker, galectin-3 (Gal3), using the anti-Gal3 antibody as a biorecognition element and surface plasmon resonance (SPR) for transducing the bioaffinity event. The immunosensing platform was built at a thiolated Au surface modified by self-assembling four bilayers of poly(diallyldimethylammonium chloride) and graphene oxide (GO), followed by the covalent attachment of 3-aminephenylboronic acid (3ABA). The importance of GO, both as the anchoring point of the antibody and as a field enhancer for improving the biosensor sensitivity, was critically discussed. The advantages of using 3ABA to orientate the anti-Gal3 antibody through the selective link to the Fc region were also demonstrated. The new platform represents an interesting alternative for the label-free biosensing of Gal3 in the whole range of clinically relevant concentrations (linear range between 10.0 and 50.0 ng mL-1, detection limit of 2.0 ng mL-1) with successful application for Gal3 biosensing in enriched human serum samples.
Fil: Primo, Emiliano Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina
Fil: Kogan, Marcelo Javier. Universidad de Chile; Chile
Fil: Verdejo, Hugo E.. Universidad de Chile; Chile. Pontificia Universidad Catolica de Chile. Facultad de Ciencias Biológicas; Chile
Fil: Bollo, Soledad. Pontificia Universidad Católica de Chile; Chile
Fil: Rubianes, María Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina - Materia
-
CARDIAC BIOMARKER
GALECTIN3
GRAPHENE OXIDE
IMMUNOSENSOR
LAYER-BY-LAYER SELF-ASSEMBLY
SPR - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
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- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/85208
Ver los metadatos del registro completo
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Label-free graphene oxide-based surface plasmon resonance immunosensor for the quantification of Galectin-3, a novel cardiac biomarkerPrimo, Emiliano NicolásKogan, Marcelo JavierVerdejo, Hugo E.Bollo, SoledadRubianes, María DoloresRivas, Gustavo AdolfoCARDIAC BIOMARKERGALECTIN3GRAPHENE OXIDEIMMUNOSENSORLAYER-BY-LAYER SELF-ASSEMBLYSPRhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1We report the first optical biosensor for the novel and important cardiac biomarker, galectin-3 (Gal3), using the anti-Gal3 antibody as a biorecognition element and surface plasmon resonance (SPR) for transducing the bioaffinity event. The immunosensing platform was built at a thiolated Au surface modified by self-assembling four bilayers of poly(diallyldimethylammonium chloride) and graphene oxide (GO), followed by the covalent attachment of 3-aminephenylboronic acid (3ABA). The importance of GO, both as the anchoring point of the antibody and as a field enhancer for improving the biosensor sensitivity, was critically discussed. The advantages of using 3ABA to orientate the anti-Gal3 antibody through the selective link to the Fc region were also demonstrated. The new platform represents an interesting alternative for the label-free biosensing of Gal3 in the whole range of clinically relevant concentrations (linear range between 10.0 and 50.0 ng mL-1, detection limit of 2.0 ng mL-1) with successful application for Gal3 biosensing in enriched human serum samples.Fil: Primo, Emiliano Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; ArgentinaFil: Kogan, Marcelo Javier. Universidad de Chile; ChileFil: Verdejo, Hugo E.. Universidad de Chile; Chile. Pontificia Universidad Catolica de Chile. Facultad de Ciencias Biológicas; ChileFil: Bollo, Soledad. Pontificia Universidad Católica de Chile; ChileFil: Rubianes, María Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaAmerican Chemical Society2018-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/85208Primo, Emiliano Nicolás; Kogan, Marcelo Javier; Verdejo, Hugo E.; Bollo, Soledad; Rubianes, María Dolores; et al.; Label-free graphene oxide-based surface plasmon resonance immunosensor for the quantification of Galectin-3, a novel cardiac biomarker; American Chemical Society; ACS Applied Materials & Interfaces; 10; 28; 7-2018; 23501-235081944-82441944-8252CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1021/acsami.8b03039info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/acsami.8b03039info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:05:25Zoai:ri.conicet.gov.ar:11336/85208instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:05:25.993CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Label-free graphene oxide-based surface plasmon resonance immunosensor for the quantification of Galectin-3, a novel cardiac biomarker |
| title |
Label-free graphene oxide-based surface plasmon resonance immunosensor for the quantification of Galectin-3, a novel cardiac biomarker |
| spellingShingle |
Label-free graphene oxide-based surface plasmon resonance immunosensor for the quantification of Galectin-3, a novel cardiac biomarker Primo, Emiliano Nicolás CARDIAC BIOMARKER GALECTIN3 GRAPHENE OXIDE IMMUNOSENSOR LAYER-BY-LAYER SELF-ASSEMBLY SPR |
| title_short |
Label-free graphene oxide-based surface plasmon resonance immunosensor for the quantification of Galectin-3, a novel cardiac biomarker |
| title_full |
Label-free graphene oxide-based surface plasmon resonance immunosensor for the quantification of Galectin-3, a novel cardiac biomarker |
| title_fullStr |
Label-free graphene oxide-based surface plasmon resonance immunosensor for the quantification of Galectin-3, a novel cardiac biomarker |
| title_full_unstemmed |
Label-free graphene oxide-based surface plasmon resonance immunosensor for the quantification of Galectin-3, a novel cardiac biomarker |
| title_sort |
Label-free graphene oxide-based surface plasmon resonance immunosensor for the quantification of Galectin-3, a novel cardiac biomarker |
| dc.creator.none.fl_str_mv |
Primo, Emiliano Nicolás Kogan, Marcelo Javier Verdejo, Hugo E. Bollo, Soledad Rubianes, María Dolores Rivas, Gustavo Adolfo |
| author |
Primo, Emiliano Nicolás |
| author_facet |
Primo, Emiliano Nicolás Kogan, Marcelo Javier Verdejo, Hugo E. Bollo, Soledad Rubianes, María Dolores Rivas, Gustavo Adolfo |
| author_role |
author |
| author2 |
Kogan, Marcelo Javier Verdejo, Hugo E. Bollo, Soledad Rubianes, María Dolores Rivas, Gustavo Adolfo |
| author2_role |
author author author author author |
| dc.subject.none.fl_str_mv |
CARDIAC BIOMARKER GALECTIN3 GRAPHENE OXIDE IMMUNOSENSOR LAYER-BY-LAYER SELF-ASSEMBLY SPR |
| topic |
CARDIAC BIOMARKER GALECTIN3 GRAPHENE OXIDE IMMUNOSENSOR LAYER-BY-LAYER SELF-ASSEMBLY SPR |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
We report the first optical biosensor for the novel and important cardiac biomarker, galectin-3 (Gal3), using the anti-Gal3 antibody as a biorecognition element and surface plasmon resonance (SPR) for transducing the bioaffinity event. The immunosensing platform was built at a thiolated Au surface modified by self-assembling four bilayers of poly(diallyldimethylammonium chloride) and graphene oxide (GO), followed by the covalent attachment of 3-aminephenylboronic acid (3ABA). The importance of GO, both as the anchoring point of the antibody and as a field enhancer for improving the biosensor sensitivity, was critically discussed. The advantages of using 3ABA to orientate the anti-Gal3 antibody through the selective link to the Fc region were also demonstrated. The new platform represents an interesting alternative for the label-free biosensing of Gal3 in the whole range of clinically relevant concentrations (linear range between 10.0 and 50.0 ng mL-1, detection limit of 2.0 ng mL-1) with successful application for Gal3 biosensing in enriched human serum samples. Fil: Primo, Emiliano Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina Fil: Kogan, Marcelo Javier. Universidad de Chile; Chile Fil: Verdejo, Hugo E.. Universidad de Chile; Chile. Pontificia Universidad Catolica de Chile. Facultad de Ciencias Biológicas; Chile Fil: Bollo, Soledad. Pontificia Universidad Católica de Chile; Chile Fil: Rubianes, María Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina |
| description |
We report the first optical biosensor for the novel and important cardiac biomarker, galectin-3 (Gal3), using the anti-Gal3 antibody as a biorecognition element and surface plasmon resonance (SPR) for transducing the bioaffinity event. The immunosensing platform was built at a thiolated Au surface modified by self-assembling four bilayers of poly(diallyldimethylammonium chloride) and graphene oxide (GO), followed by the covalent attachment of 3-aminephenylboronic acid (3ABA). The importance of GO, both as the anchoring point of the antibody and as a field enhancer for improving the biosensor sensitivity, was critically discussed. The advantages of using 3ABA to orientate the anti-Gal3 antibody through the selective link to the Fc region were also demonstrated. The new platform represents an interesting alternative for the label-free biosensing of Gal3 in the whole range of clinically relevant concentrations (linear range between 10.0 and 50.0 ng mL-1, detection limit of 2.0 ng mL-1) with successful application for Gal3 biosensing in enriched human serum samples. |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018-07 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/85208 Primo, Emiliano Nicolás; Kogan, Marcelo Javier; Verdejo, Hugo E.; Bollo, Soledad; Rubianes, María Dolores; et al.; Label-free graphene oxide-based surface plasmon resonance immunosensor for the quantification of Galectin-3, a novel cardiac biomarker; American Chemical Society; ACS Applied Materials & Interfaces; 10; 28; 7-2018; 23501-23508 1944-8244 1944-8252 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/85208 |
| identifier_str_mv |
Primo, Emiliano Nicolás; Kogan, Marcelo Javier; Verdejo, Hugo E.; Bollo, Soledad; Rubianes, María Dolores; et al.; Label-free graphene oxide-based surface plasmon resonance immunosensor for the quantification of Galectin-3, a novel cardiac biomarker; American Chemical Society; ACS Applied Materials & Interfaces; 10; 28; 7-2018; 23501-23508 1944-8244 1944-8252 CONICET Digital CONICET |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
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info:eu-repo/semantics/altIdentifier/doi/10.1021/acsami.8b03039 info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/acsami.8b03039 |
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openAccess |
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American Chemical Society |
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American Chemical Society |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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