Effect of water activity and temperature on growth and trichothecene production by Fusarium cerealis isolated from durum wheat grains

Autores
Erazo, Jessica Gabriela; Palacios, Sofia Alejendra; del Canto, Agostina; Plem, Silvana Cristina; Ramirez, Maria Laura; Torres, Adriana Mabel
Año de publicación
2022
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The major pathogen associated to fusarium head blight (FHB) is included in the Fusarium graminearum species complex. However, recently there have been reports of F. cerealis causing the disease in wheat and barley. This pathogen is able to produce deoxynivalenol (DON) and nivalenol (NIV). Nevertheless, the effect of environmental factors on growth and mycotoxin production by this species have not been studied so far. The aim of this study was to determine the effect of water activity (aw, 0.99?0.90) and temperature (15, 20, 25 and 30°C) on growth and DON and NIV production by three F. cerealis strains (RCFG6046, RCFG6029, RCFG6076) isolated from durum wheat grains. A wheat-based medium was used and adjusted to the different aW with glycerol. Plates were inoculated centrally and incubated during 28 days (three replicates per treatment). Growth rate (mm/day) was determined and mycotoxin production was analyzed after the incubation period by HPLC-UV. All strains were able to growth at all temperatures and all aW except at 0.90. Maximum growth was observed at 0.99 and 25°C and it decreased as water availability was reduced. Minimum growth was observed at 0.93 aW and 15°C. Mycotoxin production was strain dependent. Strains RCFG6046 and RCFG6076 produced both DON and NIV, being NIV the most produced. While RCFG6029 just produced DON. Strains RCFG6029 and RCFG6046 just produced DON at the optimum growth condition unlike RCFG6076 which produced only NIV. For some conditions, RCFG6076 was able to produce both toxins simultaneously in contrast to RCFG6046 which cannot produce both toxins at the same time. Maximum NIV production (9796.5 µg/kg) was observed for RCFG6076 at 0.97 aw and 30°C and the minimum level (1075.57 µg/kg) was detected at 0.95 aw and 15°C for the same strain. No NIV was detected at 0.93 aW at any temperature. DON production was observed for all conditions tested. The highest DON concentration (2954.35 µg/kg) was produced at 0.93 aw and 30°C for strain RCFG6076 while the minimum was detected at 0.99 aw and 20°C for the same strain. In conclusion, F. cerealis was able to produce both toxins in a wide range of aW and temperatures however this production was strain dependent. Maximum levels were produced during stress conditions and NIV was produced in high levels. Considering that NIV is more toxic than DON, this presents a risk for human consumption since these strains were isolated from durum wheat.
Fil: Erazo, Jessica Gabriela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Palacios, Sofia Alejendra. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: del Canto, Agostina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Plem, Silvana Cristina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Ramirez, Maria Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Torres, Adriana Mabel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
The World Mycotoxin Forum 13th Conference
Parma
Italia
Universidad de Parma
Materia
Fusarium cerealis
Ecophysiology
Deoxynivalenol
Nivalenol
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/233204
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/233204
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of water activity and temperature on growth and trichothecene production by Fusarium cerealis isolated from durum wheat grainsErazo, Jessica GabrielaPalacios, Sofia Alejendradel Canto, AgostinaPlem, Silvana CristinaRamirez, Maria LauraTorres, Adriana MabelFusarium cerealisEcophysiologyDeoxynivalenolNivalenolhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The major pathogen associated to fusarium head blight (FHB) is included in the Fusarium graminearum species complex. However, recently there have been reports of F. cerealis causing the disease in wheat and barley. This pathogen is able to produce deoxynivalenol (DON) and nivalenol (NIV). Nevertheless, the effect of environmental factors on growth and mycotoxin production by this species have not been studied so far. The aim of this study was to determine the effect of water activity (aw, 0.99?0.90) and temperature (15, 20, 25 and 30°C) on growth and DON and NIV production by three F. cerealis strains (RCFG6046, RCFG6029, RCFG6076) isolated from durum wheat grains. A wheat-based medium was used and adjusted to the different aW with glycerol. Plates were inoculated centrally and incubated during 28 days (three replicates per treatment). Growth rate (mm/day) was determined and mycotoxin production was analyzed after the incubation period by HPLC-UV. All strains were able to growth at all temperatures and all aW except at 0.90. Maximum growth was observed at 0.99 and 25°C and it decreased as water availability was reduced. Minimum growth was observed at 0.93 aW and 15°C. Mycotoxin production was strain dependent. Strains RCFG6046 and RCFG6076 produced both DON and NIV, being NIV the most produced. While RCFG6029 just produced DON. Strains RCFG6029 and RCFG6046 just produced DON at the optimum growth condition unlike RCFG6076 which produced only NIV. For some conditions, RCFG6076 was able to produce both toxins simultaneously in contrast to RCFG6046 which cannot produce both toxins at the same time. Maximum NIV production (9796.5 µg/kg) was observed for RCFG6076 at 0.97 aw and 30°C and the minimum level (1075.57 µg/kg) was detected at 0.95 aw and 15°C for the same strain. No NIV was detected at 0.93 aW at any temperature. DON production was observed for all conditions tested. The highest DON concentration (2954.35 µg/kg) was produced at 0.93 aw and 30°C for strain RCFG6076 while the minimum was detected at 0.99 aw and 20°C for the same strain. In conclusion, F. cerealis was able to produce both toxins in a wide range of aW and temperatures however this production was strain dependent. Maximum levels were produced during stress conditions and NIV was produced in high levels. Considering that NIV is more toxic than DON, this presents a risk for human consumption since these strains were isolated from durum wheat.Fil: Erazo, Jessica Gabriela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; ArgentinaFil: Palacios, Sofia Alejendra. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; ArgentinaFil: del Canto, Agostina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; ArgentinaFil: Plem, Silvana Cristina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; ArgentinaFil: Ramirez, Maria Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; ArgentinaFil: Torres, Adriana Mabel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; ArgentinaThe World Mycotoxin Forum 13th ConferenceParmaItaliaUniversidad de ParmaBastiaanse Communication2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectConferenciaBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/233204Effect of water activity and temperature on growth and trichothecene production by Fusarium cerealis isolated from durum wheat grains; The World Mycotoxin Forum 13th Conference; Parma; Italia; 2022; 150-150CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.bastiaanse-communication.com/WMF2021-2022/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:36Zoai:ri.conicet.gov.ar:11336/233204instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:36.33CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of water activity and temperature on growth and trichothecene production by Fusarium cerealis isolated from durum wheat grains
title Effect of water activity and temperature on growth and trichothecene production by Fusarium cerealis isolated from durum wheat grains
spellingShingle Effect of water activity and temperature on growth and trichothecene production by Fusarium cerealis isolated from durum wheat grains
Erazo, Jessica Gabriela
Fusarium cerealis
Ecophysiology
Deoxynivalenol
Nivalenol
title_short Effect of water activity and temperature on growth and trichothecene production by Fusarium cerealis isolated from durum wheat grains
title_full Effect of water activity and temperature on growth and trichothecene production by Fusarium cerealis isolated from durum wheat grains
title_fullStr Effect of water activity and temperature on growth and trichothecene production by Fusarium cerealis isolated from durum wheat grains
title_full_unstemmed Effect of water activity and temperature on growth and trichothecene production by Fusarium cerealis isolated from durum wheat grains
title_sort Effect of water activity and temperature on growth and trichothecene production by Fusarium cerealis isolated from durum wheat grains
dc.creator.none.fl_str_mv Erazo, Jessica Gabriela
Palacios, Sofia Alejendra
del Canto, Agostina
Plem, Silvana Cristina
Ramirez, Maria Laura
Torres, Adriana Mabel
author Erazo, Jessica Gabriela
author_facet Erazo, Jessica Gabriela
Palacios, Sofia Alejendra
del Canto, Agostina
Plem, Silvana Cristina
Ramirez, Maria Laura
Torres, Adriana Mabel
author_role author
author2 Palacios, Sofia Alejendra
del Canto, Agostina
Plem, Silvana Cristina
Ramirez, Maria Laura
Torres, Adriana Mabel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Fusarium cerealis
Ecophysiology
Deoxynivalenol
Nivalenol
topic Fusarium cerealis
Ecophysiology
Deoxynivalenol
Nivalenol
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The major pathogen associated to fusarium head blight (FHB) is included in the Fusarium graminearum species complex. However, recently there have been reports of F. cerealis causing the disease in wheat and barley. This pathogen is able to produce deoxynivalenol (DON) and nivalenol (NIV). Nevertheless, the effect of environmental factors on growth and mycotoxin production by this species have not been studied so far. The aim of this study was to determine the effect of water activity (aw, 0.99?0.90) and temperature (15, 20, 25 and 30°C) on growth and DON and NIV production by three F. cerealis strains (RCFG6046, RCFG6029, RCFG6076) isolated from durum wheat grains. A wheat-based medium was used and adjusted to the different aW with glycerol. Plates were inoculated centrally and incubated during 28 days (three replicates per treatment). Growth rate (mm/day) was determined and mycotoxin production was analyzed after the incubation period by HPLC-UV. All strains were able to growth at all temperatures and all aW except at 0.90. Maximum growth was observed at 0.99 and 25°C and it decreased as water availability was reduced. Minimum growth was observed at 0.93 aW and 15°C. Mycotoxin production was strain dependent. Strains RCFG6046 and RCFG6076 produced both DON and NIV, being NIV the most produced. While RCFG6029 just produced DON. Strains RCFG6029 and RCFG6046 just produced DON at the optimum growth condition unlike RCFG6076 which produced only NIV. For some conditions, RCFG6076 was able to produce both toxins simultaneously in contrast to RCFG6046 which cannot produce both toxins at the same time. Maximum NIV production (9796.5 µg/kg) was observed for RCFG6076 at 0.97 aw and 30°C and the minimum level (1075.57 µg/kg) was detected at 0.95 aw and 15°C for the same strain. No NIV was detected at 0.93 aW at any temperature. DON production was observed for all conditions tested. The highest DON concentration (2954.35 µg/kg) was produced at 0.93 aw and 30°C for strain RCFG6076 while the minimum was detected at 0.99 aw and 20°C for the same strain. In conclusion, F. cerealis was able to produce both toxins in a wide range of aW and temperatures however this production was strain dependent. Maximum levels were produced during stress conditions and NIV was produced in high levels. Considering that NIV is more toxic than DON, this presents a risk for human consumption since these strains were isolated from durum wheat.
Fil: Erazo, Jessica Gabriela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Palacios, Sofia Alejendra. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: del Canto, Agostina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Plem, Silvana Cristina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Ramirez, Maria Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Torres, Adriana Mabel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
The World Mycotoxin Forum 13th Conference
Parma
Italia
Universidad de Parma
description The major pathogen associated to fusarium head blight (FHB) is included in the Fusarium graminearum species complex. However, recently there have been reports of F. cerealis causing the disease in wheat and barley. This pathogen is able to produce deoxynivalenol (DON) and nivalenol (NIV). Nevertheless, the effect of environmental factors on growth and mycotoxin production by this species have not been studied so far. The aim of this study was to determine the effect of water activity (aw, 0.99?0.90) and temperature (15, 20, 25 and 30°C) on growth and DON and NIV production by three F. cerealis strains (RCFG6046, RCFG6029, RCFG6076) isolated from durum wheat grains. A wheat-based medium was used and adjusted to the different aW with glycerol. Plates were inoculated centrally and incubated during 28 days (three replicates per treatment). Growth rate (mm/day) was determined and mycotoxin production was analyzed after the incubation period by HPLC-UV. All strains were able to growth at all temperatures and all aW except at 0.90. Maximum growth was observed at 0.99 and 25°C and it decreased as water availability was reduced. Minimum growth was observed at 0.93 aW and 15°C. Mycotoxin production was strain dependent. Strains RCFG6046 and RCFG6076 produced both DON and NIV, being NIV the most produced. While RCFG6029 just produced DON. Strains RCFG6029 and RCFG6046 just produced DON at the optimum growth condition unlike RCFG6076 which produced only NIV. For some conditions, RCFG6076 was able to produce both toxins simultaneously in contrast to RCFG6046 which cannot produce both toxins at the same time. Maximum NIV production (9796.5 µg/kg) was observed for RCFG6076 at 0.97 aw and 30°C and the minimum level (1075.57 µg/kg) was detected at 0.95 aw and 15°C for the same strain. No NIV was detected at 0.93 aW at any temperature. DON production was observed for all conditions tested. The highest DON concentration (2954.35 µg/kg) was produced at 0.93 aw and 30°C for strain RCFG6076 while the minimum was detected at 0.99 aw and 20°C for the same strain. In conclusion, F. cerealis was able to produce both toxins in a wide range of aW and temperatures however this production was strain dependent. Maximum levels were produced during stress conditions and NIV was produced in high levels. Considering that NIV is more toxic than DON, this presents a risk for human consumption since these strains were isolated from durum wheat.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Conferencia
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/233204
Effect of water activity and temperature on growth and trichothecene production by Fusarium cerealis isolated from durum wheat grains; The World Mycotoxin Forum 13th Conference; Parma; Italia; 2022; 150-150
CONICET Digital
CONICET
url http://hdl.handle.net/11336/233204
identifier_str_mv Effect of water activity and temperature on growth and trichothecene production by Fusarium cerealis isolated from durum wheat grains; The World Mycotoxin Forum 13th Conference; Parma; Italia; 2022; 150-150
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.bastiaanse-communication.com/WMF2021-2022/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
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dc.format.none.fl_str_mv application/pdf
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application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Bastiaanse Communication
publisher.none.fl_str_mv Bastiaanse Communication
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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