Collagen and chitosan blends for 3D bioprinting: A rheological and printability approach

Autores
Heidenreich, Ana Carolina; Pérez Recalde, Mercedes; González Sánchez Wusener, Ana Elena; Hermida, Elida Beatriz
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Collagen and chitosan are widely employed as biomaterials, including for 3D-bioprinting. However, the use of collagen and chitosan (col:chi) blends as bioinks is still scarce. In this work, the rheology of different hydrogel precursors (0.5–1.50% w/v chi: 0.18–0.54% w/v col) was analyzed through frequency and strain sweeps, as well as at different shear rates. Col:chi blends showed a shear-thinning behavior, with viscosity values at low shear rates between 0.35 and 2.80 Pa s. Considering the strain rate determined by the applied flow in a 3D-bioprinter, precursor viscosities during the extrusion were in the interval 0.5–0.8 Pa s. Printability (Pr) was measured comparing images of the printed meshes and the corresponding CAD grid design, using photograph analysis. Col:chi 0.36:1.00 was chosen to print mono-layered scaffolds for tissue engineering (TE) because of its suitable viscosity, printability and polymer ratio content. Hydrogels were obtained through NaHCO3 nebulization and 37° incubation, and NHS/EDC were added to obtain scaffolds with improved mechanical behavior. They were stable after 44 h in PBS with collagenase at physiological level and showed no cytotoxic effect in NIH-3T3 fibroblasts.
Fil: Heidenreich, Ana Carolina. Universidad Nacional de San Martin. Escuela de Ciencia y Tecnologia. Laboratorio de Biomateriales, Biomecanica y Bioinstrumentacion.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pérez Recalde, Mercedes. Universidad Nacional de San Martin. Escuela de Ciencia y Tecnologia. Laboratorio de Biomateriales, Biomecanica y Bioinstrumentacion.; Argentina
Fil: González Sánchez Wusener, Ana Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Hermida, Elida Beatriz. Universidad Nacional de San Martin. Escuela de Ciencia y Tecnologia. Laboratorio de Biomateriales, Biomecanica y Bioinstrumentacion.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
3D BIOPRINTING
HYDROGEL PRECURSOR
RHEOLOGY
COLLAGEN
CHITOSAN
BIOINKS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/154251
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/154251
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Collagen and chitosan blends for 3D bioprinting: A rheological and printability approachHeidenreich, Ana CarolinaPérez Recalde, MercedesGonzález Sánchez Wusener, Ana ElenaHermida, Elida Beatriz3D BIOPRINTINGHYDROGEL PRECURSORRHEOLOGYCOLLAGENCHITOSANBIOINKShttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Collagen and chitosan are widely employed as biomaterials, including for 3D-bioprinting. However, the use of collagen and chitosan (col:chi) blends as bioinks is still scarce. In this work, the rheology of different hydrogel precursors (0.5–1.50% w/v chi: 0.18–0.54% w/v col) was analyzed through frequency and strain sweeps, as well as at different shear rates. Col:chi blends showed a shear-thinning behavior, with viscosity values at low shear rates between 0.35 and 2.80 Pa s. Considering the strain rate determined by the applied flow in a 3D-bioprinter, precursor viscosities during the extrusion were in the interval 0.5–0.8 Pa s. Printability (Pr) was measured comparing images of the printed meshes and the corresponding CAD grid design, using photograph analysis. Col:chi 0.36:1.00 was chosen to print mono-layered scaffolds for tissue engineering (TE) because of its suitable viscosity, printability and polymer ratio content. Hydrogels were obtained through NaHCO3 nebulization and 37° incubation, and NHS/EDC were added to obtain scaffolds with improved mechanical behavior. They were stable after 44 h in PBS with collagenase at physiological level and showed no cytotoxic effect in NIH-3T3 fibroblasts.Fil: Heidenreich, Ana Carolina. Universidad Nacional de San Martin. Escuela de Ciencia y Tecnologia. Laboratorio de Biomateriales, Biomecanica y Bioinstrumentacion.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pérez Recalde, Mercedes. Universidad Nacional de San Martin. Escuela de Ciencia y Tecnologia. Laboratorio de Biomateriales, Biomecanica y Bioinstrumentacion.; ArgentinaFil: González Sánchez Wusener, Ana Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Hermida, Elida Beatriz. Universidad Nacional de San Martin. Escuela de Ciencia y Tecnologia. Laboratorio de Biomateriales, Biomecanica y Bioinstrumentacion.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2019-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/154251Heidenreich, Ana Carolina; Pérez Recalde, Mercedes; González Sánchez Wusener, Ana Elena; Hermida, Elida Beatriz; Collagen and chitosan blends for 3D bioprinting: A rheological and printability approach; Elsevier; Polymer Testing; 82; 106297; 12-2019; 1-290142-9418CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.polymertesting.2019.106297info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0142941819320112info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:39:11Zoai:ri.conicet.gov.ar:11336/154251instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:39:12.209CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Collagen and chitosan blends for 3D bioprinting: A rheological and printability approach
title Collagen and chitosan blends for 3D bioprinting: A rheological and printability approach
spellingShingle Collagen and chitosan blends for 3D bioprinting: A rheological and printability approach
Heidenreich, Ana Carolina
3D BIOPRINTING
HYDROGEL PRECURSOR
RHEOLOGY
COLLAGEN
CHITOSAN
BIOINKS
title_short Collagen and chitosan blends for 3D bioprinting: A rheological and printability approach
title_full Collagen and chitosan blends for 3D bioprinting: A rheological and printability approach
title_fullStr Collagen and chitosan blends for 3D bioprinting: A rheological and printability approach
title_full_unstemmed Collagen and chitosan blends for 3D bioprinting: A rheological and printability approach
title_sort Collagen and chitosan blends for 3D bioprinting: A rheological and printability approach
dc.creator.none.fl_str_mv Heidenreich, Ana Carolina
Pérez Recalde, Mercedes
González Sánchez Wusener, Ana Elena
Hermida, Elida Beatriz
author Heidenreich, Ana Carolina
author_facet Heidenreich, Ana Carolina
Pérez Recalde, Mercedes
González Sánchez Wusener, Ana Elena
Hermida, Elida Beatriz
author_role author
author2 Pérez Recalde, Mercedes
González Sánchez Wusener, Ana Elena
Hermida, Elida Beatriz
author2_role author
author
author
dc.subject.none.fl_str_mv 3D BIOPRINTING
HYDROGEL PRECURSOR
RHEOLOGY
COLLAGEN
CHITOSAN
BIOINKS
topic 3D BIOPRINTING
HYDROGEL PRECURSOR
RHEOLOGY
COLLAGEN
CHITOSAN
BIOINKS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Collagen and chitosan are widely employed as biomaterials, including for 3D-bioprinting. However, the use of collagen and chitosan (col:chi) blends as bioinks is still scarce. In this work, the rheology of different hydrogel precursors (0.5–1.50% w/v chi: 0.18–0.54% w/v col) was analyzed through frequency and strain sweeps, as well as at different shear rates. Col:chi blends showed a shear-thinning behavior, with viscosity values at low shear rates between 0.35 and 2.80 Pa s. Considering the strain rate determined by the applied flow in a 3D-bioprinter, precursor viscosities during the extrusion were in the interval 0.5–0.8 Pa s. Printability (Pr) was measured comparing images of the printed meshes and the corresponding CAD grid design, using photograph analysis. Col:chi 0.36:1.00 was chosen to print mono-layered scaffolds for tissue engineering (TE) because of its suitable viscosity, printability and polymer ratio content. Hydrogels were obtained through NaHCO3 nebulization and 37° incubation, and NHS/EDC were added to obtain scaffolds with improved mechanical behavior. They were stable after 44 h in PBS with collagenase at physiological level and showed no cytotoxic effect in NIH-3T3 fibroblasts.
Fil: Heidenreich, Ana Carolina. Universidad Nacional de San Martin. Escuela de Ciencia y Tecnologia. Laboratorio de Biomateriales, Biomecanica y Bioinstrumentacion.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pérez Recalde, Mercedes. Universidad Nacional de San Martin. Escuela de Ciencia y Tecnologia. Laboratorio de Biomateriales, Biomecanica y Bioinstrumentacion.; Argentina
Fil: González Sánchez Wusener, Ana Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Hermida, Elida Beatriz. Universidad Nacional de San Martin. Escuela de Ciencia y Tecnologia. Laboratorio de Biomateriales, Biomecanica y Bioinstrumentacion.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Collagen and chitosan are widely employed as biomaterials, including for 3D-bioprinting. However, the use of collagen and chitosan (col:chi) blends as bioinks is still scarce. In this work, the rheology of different hydrogel precursors (0.5–1.50% w/v chi: 0.18–0.54% w/v col) was analyzed through frequency and strain sweeps, as well as at different shear rates. Col:chi blends showed a shear-thinning behavior, with viscosity values at low shear rates between 0.35 and 2.80 Pa s. Considering the strain rate determined by the applied flow in a 3D-bioprinter, precursor viscosities during the extrusion were in the interval 0.5–0.8 Pa s. Printability (Pr) was measured comparing images of the printed meshes and the corresponding CAD grid design, using photograph analysis. Col:chi 0.36:1.00 was chosen to print mono-layered scaffolds for tissue engineering (TE) because of its suitable viscosity, printability and polymer ratio content. Hydrogels were obtained through NaHCO3 nebulization and 37° incubation, and NHS/EDC were added to obtain scaffolds with improved mechanical behavior. They were stable after 44 h in PBS with collagenase at physiological level and showed no cytotoxic effect in NIH-3T3 fibroblasts.
publishDate 2019
dc.date.none.fl_str_mv 2019-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/154251
Heidenreich, Ana Carolina; Pérez Recalde, Mercedes; González Sánchez Wusener, Ana Elena; Hermida, Elida Beatriz; Collagen and chitosan blends for 3D bioprinting: A rheological and printability approach; Elsevier; Polymer Testing; 82; 106297; 12-2019; 1-29
0142-9418
CONICET Digital
CONICET
url http://hdl.handle.net/11336/154251
identifier_str_mv Heidenreich, Ana Carolina; Pérez Recalde, Mercedes; González Sánchez Wusener, Ana Elena; Hermida, Elida Beatriz; Collagen and chitosan blends for 3D bioprinting: A rheological and printability approach; Elsevier; Polymer Testing; 82; 106297; 12-2019; 1-29
0142-9418
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.polymertesting.2019.106297
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0142941819320112
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782054808682496
score 12.982451

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