Collagen and chitosan blends for 3D bioprinting: A rheological and printability approach
- Autores
- Heidenreich, Ana Carolina; Pérez Recalde, Mercedes; González Sánchez Wusener, Ana Elena; Hermida, Elida Beatriz
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Collagen and chitosan are widely employed as biomaterials, including for 3D-bioprinting. However, the use of collagen and chitosan (col:chi) blends as bioinks is still scarce. In this work, the rheology of different hydrogel precursors (0.5–1.50% w/v chi: 0.18–0.54% w/v col) was analyzed through frequency and strain sweeps, as well as at different shear rates. Col:chi blends showed a shear-thinning behavior, with viscosity values at low shear rates between 0.35 and 2.80 Pa s. Considering the strain rate determined by the applied flow in a 3D-bioprinter, precursor viscosities during the extrusion were in the interval 0.5–0.8 Pa s. Printability (Pr) was measured comparing images of the printed meshes and the corresponding CAD grid design, using photograph analysis. Col:chi 0.36:1.00 was chosen to print mono-layered scaffolds for tissue engineering (TE) because of its suitable viscosity, printability and polymer ratio content. Hydrogels were obtained through NaHCO3 nebulization and 37° incubation, and NHS/EDC were added to obtain scaffolds with improved mechanical behavior. They were stable after 44 h in PBS with collagenase at physiological level and showed no cytotoxic effect in NIH-3T3 fibroblasts.
Fil: Heidenreich, Ana Carolina. Universidad Nacional de San Martin. Escuela de Ciencia y Tecnologia. Laboratorio de Biomateriales, Biomecanica y Bioinstrumentacion.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pérez Recalde, Mercedes. Universidad Nacional de San Martin. Escuela de Ciencia y Tecnologia. Laboratorio de Biomateriales, Biomecanica y Bioinstrumentacion.; Argentina
Fil: González Sánchez Wusener, Ana Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Hermida, Elida Beatriz. Universidad Nacional de San Martin. Escuela de Ciencia y Tecnologia. Laboratorio de Biomateriales, Biomecanica y Bioinstrumentacion.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Materia
-
3D BIOPRINTING
HYDROGEL PRECURSOR
RHEOLOGY
COLLAGEN
CHITOSAN
BIOINKS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/154251
Ver los metadatos del registro completo
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Collagen and chitosan blends for 3D bioprinting: A rheological and printability approachHeidenreich, Ana CarolinaPérez Recalde, MercedesGonzález Sánchez Wusener, Ana ElenaHermida, Elida Beatriz3D BIOPRINTINGHYDROGEL PRECURSORRHEOLOGYCOLLAGENCHITOSANBIOINKShttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Collagen and chitosan are widely employed as biomaterials, including for 3D-bioprinting. However, the use of collagen and chitosan (col:chi) blends as bioinks is still scarce. In this work, the rheology of different hydrogel precursors (0.5–1.50% w/v chi: 0.18–0.54% w/v col) was analyzed through frequency and strain sweeps, as well as at different shear rates. Col:chi blends showed a shear-thinning behavior, with viscosity values at low shear rates between 0.35 and 2.80 Pa s. Considering the strain rate determined by the applied flow in a 3D-bioprinter, precursor viscosities during the extrusion were in the interval 0.5–0.8 Pa s. Printability (Pr) was measured comparing images of the printed meshes and the corresponding CAD grid design, using photograph analysis. Col:chi 0.36:1.00 was chosen to print mono-layered scaffolds for tissue engineering (TE) because of its suitable viscosity, printability and polymer ratio content. Hydrogels were obtained through NaHCO3 nebulization and 37° incubation, and NHS/EDC were added to obtain scaffolds with improved mechanical behavior. They were stable after 44 h in PBS with collagenase at physiological level and showed no cytotoxic effect in NIH-3T3 fibroblasts.Fil: Heidenreich, Ana Carolina. Universidad Nacional de San Martin. Escuela de Ciencia y Tecnologia. Laboratorio de Biomateriales, Biomecanica y Bioinstrumentacion.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pérez Recalde, Mercedes. Universidad Nacional de San Martin. Escuela de Ciencia y Tecnologia. Laboratorio de Biomateriales, Biomecanica y Bioinstrumentacion.; ArgentinaFil: González Sánchez Wusener, Ana Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Hermida, Elida Beatriz. Universidad Nacional de San Martin. Escuela de Ciencia y Tecnologia. Laboratorio de Biomateriales, Biomecanica y Bioinstrumentacion.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2019-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/154251Heidenreich, Ana Carolina; Pérez Recalde, Mercedes; González Sánchez Wusener, Ana Elena; Hermida, Elida Beatriz; Collagen and chitosan blends for 3D bioprinting: A rheological and printability approach; Elsevier; Polymer Testing; 82; 106297; 12-2019; 1-290142-9418CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.polymertesting.2019.106297info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0142941819320112info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:09:20Zoai:ri.conicet.gov.ar:11336/154251instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:09:20.259CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Collagen and chitosan blends for 3D bioprinting: A rheological and printability approach |
title |
Collagen and chitosan blends for 3D bioprinting: A rheological and printability approach |
spellingShingle |
Collagen and chitosan blends for 3D bioprinting: A rheological and printability approach Heidenreich, Ana Carolina 3D BIOPRINTING HYDROGEL PRECURSOR RHEOLOGY COLLAGEN CHITOSAN BIOINKS |
title_short |
Collagen and chitosan blends for 3D bioprinting: A rheological and printability approach |
title_full |
Collagen and chitosan blends for 3D bioprinting: A rheological and printability approach |
title_fullStr |
Collagen and chitosan blends for 3D bioprinting: A rheological and printability approach |
title_full_unstemmed |
Collagen and chitosan blends for 3D bioprinting: A rheological and printability approach |
title_sort |
Collagen and chitosan blends for 3D bioprinting: A rheological and printability approach |
dc.creator.none.fl_str_mv |
Heidenreich, Ana Carolina Pérez Recalde, Mercedes González Sánchez Wusener, Ana Elena Hermida, Elida Beatriz |
author |
Heidenreich, Ana Carolina |
author_facet |
Heidenreich, Ana Carolina Pérez Recalde, Mercedes González Sánchez Wusener, Ana Elena Hermida, Elida Beatriz |
author_role |
author |
author2 |
Pérez Recalde, Mercedes González Sánchez Wusener, Ana Elena Hermida, Elida Beatriz |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
3D BIOPRINTING HYDROGEL PRECURSOR RHEOLOGY COLLAGEN CHITOSAN BIOINKS |
topic |
3D BIOPRINTING HYDROGEL PRECURSOR RHEOLOGY COLLAGEN CHITOSAN BIOINKS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.4 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Collagen and chitosan are widely employed as biomaterials, including for 3D-bioprinting. However, the use of collagen and chitosan (col:chi) blends as bioinks is still scarce. In this work, the rheology of different hydrogel precursors (0.5–1.50% w/v chi: 0.18–0.54% w/v col) was analyzed through frequency and strain sweeps, as well as at different shear rates. Col:chi blends showed a shear-thinning behavior, with viscosity values at low shear rates between 0.35 and 2.80 Pa s. Considering the strain rate determined by the applied flow in a 3D-bioprinter, precursor viscosities during the extrusion were in the interval 0.5–0.8 Pa s. Printability (Pr) was measured comparing images of the printed meshes and the corresponding CAD grid design, using photograph analysis. Col:chi 0.36:1.00 was chosen to print mono-layered scaffolds for tissue engineering (TE) because of its suitable viscosity, printability and polymer ratio content. Hydrogels were obtained through NaHCO3 nebulization and 37° incubation, and NHS/EDC were added to obtain scaffolds with improved mechanical behavior. They were stable after 44 h in PBS with collagenase at physiological level and showed no cytotoxic effect in NIH-3T3 fibroblasts. Fil: Heidenreich, Ana Carolina. Universidad Nacional de San Martin. Escuela de Ciencia y Tecnologia. Laboratorio de Biomateriales, Biomecanica y Bioinstrumentacion.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pérez Recalde, Mercedes. Universidad Nacional de San Martin. Escuela de Ciencia y Tecnologia. Laboratorio de Biomateriales, Biomecanica y Bioinstrumentacion.; Argentina Fil: González Sánchez Wusener, Ana Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina Fil: Hermida, Elida Beatriz. Universidad Nacional de San Martin. Escuela de Ciencia y Tecnologia. Laboratorio de Biomateriales, Biomecanica y Bioinstrumentacion.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
Collagen and chitosan are widely employed as biomaterials, including for 3D-bioprinting. However, the use of collagen and chitosan (col:chi) blends as bioinks is still scarce. In this work, the rheology of different hydrogel precursors (0.5–1.50% w/v chi: 0.18–0.54% w/v col) was analyzed through frequency and strain sweeps, as well as at different shear rates. Col:chi blends showed a shear-thinning behavior, with viscosity values at low shear rates between 0.35 and 2.80 Pa s. Considering the strain rate determined by the applied flow in a 3D-bioprinter, precursor viscosities during the extrusion were in the interval 0.5–0.8 Pa s. Printability (Pr) was measured comparing images of the printed meshes and the corresponding CAD grid design, using photograph analysis. Col:chi 0.36:1.00 was chosen to print mono-layered scaffolds for tissue engineering (TE) because of its suitable viscosity, printability and polymer ratio content. Hydrogels were obtained through NaHCO3 nebulization and 37° incubation, and NHS/EDC were added to obtain scaffolds with improved mechanical behavior. They were stable after 44 h in PBS with collagenase at physiological level and showed no cytotoxic effect in NIH-3T3 fibroblasts. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/154251 Heidenreich, Ana Carolina; Pérez Recalde, Mercedes; González Sánchez Wusener, Ana Elena; Hermida, Elida Beatriz; Collagen and chitosan blends for 3D bioprinting: A rheological and printability approach; Elsevier; Polymer Testing; 82; 106297; 12-2019; 1-29 0142-9418 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/154251 |
identifier_str_mv |
Heidenreich, Ana Carolina; Pérez Recalde, Mercedes; González Sánchez Wusener, Ana Elena; Hermida, Elida Beatriz; Collagen and chitosan blends for 3D bioprinting: A rheological and printability approach; Elsevier; Polymer Testing; 82; 106297; 12-2019; 1-29 0142-9418 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.polymertesting.2019.106297 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0142941819320112 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613970175983616 |
score |
13.070432 |