Sedative-like effect of intraperitoneal GABA administration in the open field test

Autores
Gargiulo, Augusto Pascual Ítalo; Marquez Herrero, Santiago; Romanowicz, Esteban Alejandro; Guevara, Manuel Alejandro; Landa, Adriana Inés; Lafuente, José Vicente; Mesones, Humberto Luis; Gargiulo, Pascual Angel
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Gamma-Amino Butyric Acid (GABA) is the main inhibitor neurotransmitter of the Central Nervous System (CNS). Its peripheral administration has been matter of discussion. On the one hand, it has been reported that it does not cross the Blood-Brain Barrier (BBB), and, on the other hand, it has been associated with multiple therapeutic regimens and supplements by peripheral administration. The aim of the present study is to elucidate the possibility of a central sedative effect when administered peripherally. An experimental cohort of 90-day-old Holtzman male rats weighing 240-270 g was used. It was divided into 2 groups: saline-controls (n = 9) and GABA treated rats (12.5 mg/kg, n = 9). Both groups were intraperitoneally injected. The motor behavioral patterns displayed in the Opto Varimex (OVM) were studied. Vertical, horizontal, ambulatory and non-ambulatory movements and the number of movements were recorded in an automated way. Horizontal movements constitute the integration of ambulatory and non-ambulatory movements. Student t test was used comparing groups. In this experiment, there were non-significant downward trends in vertical, ambulatory, non-ambulatory and number of movements. Ambulatory and non-ambulatory tendencies acquired significance when treated together as horizontal movements (p < 0.05). We may conclude that peripheral administration of GABA produced a decrease of the horizontal movements in the open field test. It may be interpreted as a sedative effect, suggesting a passage of GABA through BBB, with central effects. However, there are several alternative possibilities to explain present findings. Other experiments will elucidate the implications or scope of the present findings.
Fil: Gargiulo, Augusto Pascual Ítalo. Laboratorio de Neurociencias y Psicología Experimental; Argentina. Universidad Católica de Cuyo - Sede San Juan. Facultad de Ciencias Médicas; Argentina
Fil: Marquez Herrero, Santiago. Laboratorio de Neurociencias y Psicología Experimental; Argentina. Universidad Católica de Cuyo - Sede San Juan. Facultad de Ciencias Médicas; Argentina
Fil: Romanowicz, Esteban Alejandro. Laboratorio de Neurociencias y Psicología Experimental; Argentina. Universidad Católica de Cuyo - Sede San Juan. Facultad de Ciencias Médicas; Argentina
Fil: Guevara, Manuel Alejandro. Laboratorio de Neurociencias y Psicología Experimental; Argentina. Universidad Católica de Cuyo - Sede San Juan. Facultad de Ciencias Médicas; Argentina
Fil: Landa, Adriana Inés. Laboratorio de Neurociencias y Psicología Experimental; Argentina. Universidad Católica de Cuyo - Sede San Juan. Facultad de Ciencias Médicas; Argentina
Fil: Lafuente, José Vicente. Universidad del País Vasco; España
Fil: Mesones, Humberto Luis. Laboratorio de Neurociencias y Psicología Experimental; Argentina. Fundación Instituto de Neurobiología; Argentina. Universidad Católica de Cuyo - Sede San Juan. Facultad de Ciencias Médicas; Argentina
Fil: Gargiulo, Pascual Angel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Laboratorio de Neurociencias y Psicología Experimental; Argentina. Universidad Católica de Cuyo - Sede San Juan. Facultad de Ciencias Médicas; Argentina
Materia
GAMMA-AMINO-BUTIRIC ACID
GABA
SEDATION
BLOOD BRAIN BARRIER
RAT
BEAHVAIOR
LOCOMOTOR
ACTIVITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/100382
Acceder
id CONICETDig_382c8493fcca38cb5c9dd39de47d9d9a
oai_identifier_str oai:ri.conicet.gov.ar:11336/100382
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Sedative-like effect of intraperitoneal GABA administration in the open field testGargiulo, Augusto Pascual ÍtaloMarquez Herrero, SantiagoRomanowicz, Esteban AlejandroGuevara, Manuel AlejandroLanda, Adriana InésLafuente, José VicenteMesones, Humberto LuisGargiulo, Pascual AngelGAMMA-AMINO-BUTIRIC ACIDGABASEDATIONBLOOD BRAIN BARRIERRATBEAHVAIORLOCOMOTORACTIVITYhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Gamma-Amino Butyric Acid (GABA) is the main inhibitor neurotransmitter of the Central Nervous System (CNS). Its peripheral administration has been matter of discussion. On the one hand, it has been reported that it does not cross the Blood-Brain Barrier (BBB), and, on the other hand, it has been associated with multiple therapeutic regimens and supplements by peripheral administration. The aim of the present study is to elucidate the possibility of a central sedative effect when administered peripherally. An experimental cohort of 90-day-old Holtzman male rats weighing 240-270 g was used. It was divided into 2 groups: saline-controls (n = 9) and GABA treated rats (12.5 mg/kg, n = 9). Both groups were intraperitoneally injected. The motor behavioral patterns displayed in the Opto Varimex (OVM) were studied. Vertical, horizontal, ambulatory and non-ambulatory movements and the number of movements were recorded in an automated way. Horizontal movements constitute the integration of ambulatory and non-ambulatory movements. Student t test was used comparing groups. In this experiment, there were non-significant downward trends in vertical, ambulatory, non-ambulatory and number of movements. Ambulatory and non-ambulatory tendencies acquired significance when treated together as horizontal movements (p < 0.05). We may conclude that peripheral administration of GABA produced a decrease of the horizontal movements in the open field test. It may be interpreted as a sedative effect, suggesting a passage of GABA through BBB, with central effects. However, there are several alternative possibilities to explain present findings. Other experiments will elucidate the implications or scope of the present findings.Fil: Gargiulo, Augusto Pascual Ítalo. Laboratorio de Neurociencias y Psicología Experimental; Argentina. Universidad Católica de Cuyo - Sede San Juan. Facultad de Ciencias Médicas; ArgentinaFil: Marquez Herrero, Santiago. Laboratorio de Neurociencias y Psicología Experimental; Argentina. Universidad Católica de Cuyo - Sede San Juan. Facultad de Ciencias Médicas; ArgentinaFil: Romanowicz, Esteban Alejandro. Laboratorio de Neurociencias y Psicología Experimental; Argentina. Universidad Católica de Cuyo - Sede San Juan. Facultad de Ciencias Médicas; ArgentinaFil: Guevara, Manuel Alejandro. Laboratorio de Neurociencias y Psicología Experimental; Argentina. Universidad Católica de Cuyo - Sede San Juan. Facultad de Ciencias Médicas; ArgentinaFil: Landa, Adriana Inés. Laboratorio de Neurociencias y Psicología Experimental; Argentina. Universidad Católica de Cuyo - Sede San Juan. Facultad de Ciencias Médicas; ArgentinaFil: Lafuente, José Vicente. Universidad del País Vasco; EspañaFil: Mesones, Humberto Luis. Laboratorio de Neurociencias y Psicología Experimental; Argentina. Fundación Instituto de Neurobiología; Argentina. Universidad Católica de Cuyo - Sede San Juan. Facultad de Ciencias Médicas; ArgentinaFil: Gargiulo, Pascual Angel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Laboratorio de Neurociencias y Psicología Experimental; Argentina. Universidad Católica de Cuyo - Sede San Juan. Facultad de Ciencias Médicas; ArgentinaRede Brasileira de Informações Biológicas - Rebibio2016-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/100382Gargiulo, Augusto Pascual Ítalo; Marquez Herrero, Santiago; Romanowicz, Esteban Alejandro; Guevara, Manuel Alejandro; Landa, Adriana Inés; et al.; Sedative-like effect of intraperitoneal GABA administration in the open field test; Rede Brasileira de Informações Biológicas - Rebibio; Brazilian Journal of Biological Sciences; 03; 06; 12-2016; 257-2622358-2731CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.21472/bjbs.030602info:eu-repo/semantics/altIdentifier/url/http://revista.rebibio.net/v3n6/v03n06a02.htmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:20:43Zoai:ri.conicet.gov.ar:11336/100382instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:20:43.671CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sedative-like effect of intraperitoneal GABA administration in the open field test
title Sedative-like effect of intraperitoneal GABA administration in the open field test
spellingShingle Sedative-like effect of intraperitoneal GABA administration in the open field test
Gargiulo, Augusto Pascual Ítalo
GAMMA-AMINO-BUTIRIC ACID
GABA
SEDATION
BLOOD BRAIN BARRIER
RAT
BEAHVAIOR
LOCOMOTOR
ACTIVITY
title_short Sedative-like effect of intraperitoneal GABA administration in the open field test
title_full Sedative-like effect of intraperitoneal GABA administration in the open field test
title_fullStr Sedative-like effect of intraperitoneal GABA administration in the open field test
title_full_unstemmed Sedative-like effect of intraperitoneal GABA administration in the open field test
title_sort Sedative-like effect of intraperitoneal GABA administration in the open field test
dc.creator.none.fl_str_mv Gargiulo, Augusto Pascual Ítalo
Marquez Herrero, Santiago
Romanowicz, Esteban Alejandro
Guevara, Manuel Alejandro
Landa, Adriana Inés
Lafuente, José Vicente
Mesones, Humberto Luis
Gargiulo, Pascual Angel
author Gargiulo, Augusto Pascual Ítalo
author_facet Gargiulo, Augusto Pascual Ítalo
Marquez Herrero, Santiago
Romanowicz, Esteban Alejandro
Guevara, Manuel Alejandro
Landa, Adriana Inés
Lafuente, José Vicente
Mesones, Humberto Luis
Gargiulo, Pascual Angel
author_role author
author2 Marquez Herrero, Santiago
Romanowicz, Esteban Alejandro
Guevara, Manuel Alejandro
Landa, Adriana Inés
Lafuente, José Vicente
Mesones, Humberto Luis
Gargiulo, Pascual Angel
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv GAMMA-AMINO-BUTIRIC ACID
GABA
SEDATION
BLOOD BRAIN BARRIER
RAT
BEAHVAIOR
LOCOMOTOR
ACTIVITY
topic GAMMA-AMINO-BUTIRIC ACID
GABA
SEDATION
BLOOD BRAIN BARRIER
RAT
BEAHVAIOR
LOCOMOTOR
ACTIVITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Gamma-Amino Butyric Acid (GABA) is the main inhibitor neurotransmitter of the Central Nervous System (CNS). Its peripheral administration has been matter of discussion. On the one hand, it has been reported that it does not cross the Blood-Brain Barrier (BBB), and, on the other hand, it has been associated with multiple therapeutic regimens and supplements by peripheral administration. The aim of the present study is to elucidate the possibility of a central sedative effect when administered peripherally. An experimental cohort of 90-day-old Holtzman male rats weighing 240-270 g was used. It was divided into 2 groups: saline-controls (n = 9) and GABA treated rats (12.5 mg/kg, n = 9). Both groups were intraperitoneally injected. The motor behavioral patterns displayed in the Opto Varimex (OVM) were studied. Vertical, horizontal, ambulatory and non-ambulatory movements and the number of movements were recorded in an automated way. Horizontal movements constitute the integration of ambulatory and non-ambulatory movements. Student t test was used comparing groups. In this experiment, there were non-significant downward trends in vertical, ambulatory, non-ambulatory and number of movements. Ambulatory and non-ambulatory tendencies acquired significance when treated together as horizontal movements (p < 0.05). We may conclude that peripheral administration of GABA produced a decrease of the horizontal movements in the open field test. It may be interpreted as a sedative effect, suggesting a passage of GABA through BBB, with central effects. However, there are several alternative possibilities to explain present findings. Other experiments will elucidate the implications or scope of the present findings.
Fil: Gargiulo, Augusto Pascual Ítalo. Laboratorio de Neurociencias y Psicología Experimental; Argentina. Universidad Católica de Cuyo - Sede San Juan. Facultad de Ciencias Médicas; Argentina
Fil: Marquez Herrero, Santiago. Laboratorio de Neurociencias y Psicología Experimental; Argentina. Universidad Católica de Cuyo - Sede San Juan. Facultad de Ciencias Médicas; Argentina
Fil: Romanowicz, Esteban Alejandro. Laboratorio de Neurociencias y Psicología Experimental; Argentina. Universidad Católica de Cuyo - Sede San Juan. Facultad de Ciencias Médicas; Argentina
Fil: Guevara, Manuel Alejandro. Laboratorio de Neurociencias y Psicología Experimental; Argentina. Universidad Católica de Cuyo - Sede San Juan. Facultad de Ciencias Médicas; Argentina
Fil: Landa, Adriana Inés. Laboratorio de Neurociencias y Psicología Experimental; Argentina. Universidad Católica de Cuyo - Sede San Juan. Facultad de Ciencias Médicas; Argentina
Fil: Lafuente, José Vicente. Universidad del País Vasco; España
Fil: Mesones, Humberto Luis. Laboratorio de Neurociencias y Psicología Experimental; Argentina. Fundación Instituto de Neurobiología; Argentina. Universidad Católica de Cuyo - Sede San Juan. Facultad de Ciencias Médicas; Argentina
Fil: Gargiulo, Pascual Angel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Laboratorio de Neurociencias y Psicología Experimental; Argentina. Universidad Católica de Cuyo - Sede San Juan. Facultad de Ciencias Médicas; Argentina
description Gamma-Amino Butyric Acid (GABA) is the main inhibitor neurotransmitter of the Central Nervous System (CNS). Its peripheral administration has been matter of discussion. On the one hand, it has been reported that it does not cross the Blood-Brain Barrier (BBB), and, on the other hand, it has been associated with multiple therapeutic regimens and supplements by peripheral administration. The aim of the present study is to elucidate the possibility of a central sedative effect when administered peripherally. An experimental cohort of 90-day-old Holtzman male rats weighing 240-270 g was used. It was divided into 2 groups: saline-controls (n = 9) and GABA treated rats (12.5 mg/kg, n = 9). Both groups were intraperitoneally injected. The motor behavioral patterns displayed in the Opto Varimex (OVM) were studied. Vertical, horizontal, ambulatory and non-ambulatory movements and the number of movements were recorded in an automated way. Horizontal movements constitute the integration of ambulatory and non-ambulatory movements. Student t test was used comparing groups. In this experiment, there were non-significant downward trends in vertical, ambulatory, non-ambulatory and number of movements. Ambulatory and non-ambulatory tendencies acquired significance when treated together as horizontal movements (p < 0.05). We may conclude that peripheral administration of GABA produced a decrease of the horizontal movements in the open field test. It may be interpreted as a sedative effect, suggesting a passage of GABA through BBB, with central effects. However, there are several alternative possibilities to explain present findings. Other experiments will elucidate the implications or scope of the present findings.
publishDate 2016
dc.date.none.fl_str_mv 2016-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/100382
Gargiulo, Augusto Pascual Ítalo; Marquez Herrero, Santiago; Romanowicz, Esteban Alejandro; Guevara, Manuel Alejandro; Landa, Adriana Inés; et al.; Sedative-like effect of intraperitoneal GABA administration in the open field test; Rede Brasileira de Informações Biológicas - Rebibio; Brazilian Journal of Biological Sciences; 03; 06; 12-2016; 257-262
2358-2731
CONICET Digital
CONICET
url http://hdl.handle.net/11336/100382
identifier_str_mv Gargiulo, Augusto Pascual Ítalo; Marquez Herrero, Santiago; Romanowicz, Esteban Alejandro; Guevara, Manuel Alejandro; Landa, Adriana Inés; et al.; Sedative-like effect of intraperitoneal GABA administration in the open field test; Rede Brasileira de Informações Biológicas - Rebibio; Brazilian Journal of Biological Sciences; 03; 06; 12-2016; 257-262
2358-2731
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.21472/bjbs.030602
info:eu-repo/semantics/altIdentifier/url/http://revista.rebibio.net/v3n6/v03n06a02.html
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Rede Brasileira de Informações Biológicas - Rebibio
publisher.none.fl_str_mv Rede Brasileira de Informações Biológicas - Rebibio
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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