Identification of potential proteins involved in angiogenesis associated with cervical cancer using proteomics and bioinformatics approaches

Autores
Valero, Victoria; Homann, Luana Micaela; Carriere, Pedro Matias; Novoa Díaz, María Belén; Gentili, Claudia Rosana; Calvo, Natalia Graciela
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Angiogenesis is the growth of blood vessels from the existing vasculature and is essential in the progression of cervical cancer (CC), the fourth most common tumor in women worldwide. This process studies in search of potential biomarkers and therapeutic targets since the endothelial cells that form the abnormal tumor vasculature are characterized by changes at the protein level when are regulated by tumor and microenvironmental factors. The objective of this work was to identify potential proteins involved in the response of endothelial cells to soluble factors released by tumor cells derived from CC, using proteomics and bioinformatics approaches. We previously observed that treatment with conditioned media from CC HeLa cells (TCMs) for 24 h increases the number of endothelial HMEC-1 cells. In this work, the proteome response of HMEC-1 cells was studied underthese experimental conditions, performing a Label-Free quantitative (LFQ) mass spectrometry (MS) at the CEQUIBIEM Proteomics Center. Proteins were identified and quantified with the Proteome Discoverer software and the Uniprot database. Also, a more in-depth statistical study was performed using the Perseus software. Proteomic analysis revealed 26 proteins with increased expression levels in endothelial cells treated with TCM (P ≤ 0.05). Then, to evaluate the biological characteristics of these proteins, they were classified using the PANTHER analysis tool, according to their molecular function and biological processes. As a result of this study, catalytic activity was the most represented molecular function (11/26), followed by binding (4/26). Respect to biological processes, proteins were mainly classified into cellular processes (12/26) and energy metabolism(11/26). This analysis suggests that factors released by tumor cells mainly increase the expression of proteins involved in metabolic processes in endothelial cells. Within these proteins, the probable ATP-dependent RNA helicase DDX47 showed the greatest magnitude of change (> 2). DDX47is related to rRNA processing and ribosome biogenesis, which are processes associated with cell proliferation and cancer progression. Furthermore, ribosomal activity is also a critical regulator of metabolism. These results highlight the use of Label-Free spectrometry and bioinformatics approaches in an initial phase of discovery of potential proteins involved in cancer and suggest the potential role of DDX47 in angiogenesis associated with CC.
Fil: Valero, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Homann, Luana Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Carriere, Pedro Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Novoa Díaz, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Gentili, Claudia Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Calvo, Natalia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
IV Reunión Conjunta de Sociedades de Biología de la República Argentina
Argentina
Sociedad Argentina de Biología
Materia
CERVICAL CANCER
ENDOTHELIAL CELLS
ANGIOGENESIS
PROTEOME
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/231401
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/231401
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Identification of potential proteins involved in angiogenesis associated with cervical cancer using proteomics and bioinformatics approachesValero, VictoriaHomann, Luana MicaelaCarriere, Pedro MatiasNovoa Díaz, María BelénGentili, Claudia RosanaCalvo, Natalia GracielaCERVICAL CANCERENDOTHELIAL CELLSANGIOGENESISPROTEOMEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Angiogenesis is the growth of blood vessels from the existing vasculature and is essential in the progression of cervical cancer (CC), the fourth most common tumor in women worldwide. This process studies in search of potential biomarkers and therapeutic targets since the endothelial cells that form the abnormal tumor vasculature are characterized by changes at the protein level when are regulated by tumor and microenvironmental factors. The objective of this work was to identify potential proteins involved in the response of endothelial cells to soluble factors released by tumor cells derived from CC, using proteomics and bioinformatics approaches. We previously observed that treatment with conditioned media from CC HeLa cells (TCMs) for 24 h increases the number of endothelial HMEC-1 cells. In this work, the proteome response of HMEC-1 cells was studied underthese experimental conditions, performing a Label-Free quantitative (LFQ) mass spectrometry (MS) at the CEQUIBIEM Proteomics Center. Proteins were identified and quantified with the Proteome Discoverer software and the Uniprot database. Also, a more in-depth statistical study was performed using the Perseus software. Proteomic analysis revealed 26 proteins with increased expression levels in endothelial cells treated with TCM (P ≤ 0.05). Then, to evaluate the biological characteristics of these proteins, they were classified using the PANTHER analysis tool, according to their molecular function and biological processes. As a result of this study, catalytic activity was the most represented molecular function (11/26), followed by binding (4/26). Respect to biological processes, proteins were mainly classified into cellular processes (12/26) and energy metabolism(11/26). This analysis suggests that factors released by tumor cells mainly increase the expression of proteins involved in metabolic processes in endothelial cells. Within these proteins, the probable ATP-dependent RNA helicase DDX47 showed the greatest magnitude of change (> 2). DDX47is related to rRNA processing and ribosome biogenesis, which are processes associated with cell proliferation and cancer progression. Furthermore, ribosomal activity is also a critical regulator of metabolism. These results highlight the use of Label-Free spectrometry and bioinformatics approaches in an initial phase of discovery of potential proteins involved in cancer and suggest the potential role of DDX47 in angiogenesis associated with CC.Fil: Valero, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Homann, Luana Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Carriere, Pedro Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Novoa Díaz, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Gentili, Claudia Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Calvo, Natalia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaIV Reunión Conjunta de Sociedades de Biología de la República ArgentinaArgentinaSociedad Argentina de BiologíaTech Science PressParborell, Maria Fernanda Agustina2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/231401Identification of potential proteins involved in angiogenesis associated with cervical cancer using proteomics and bioinformatics approaches; IV Reunión Conjunta de Sociedades de Biología de la República Argentina; Argentina; 2020; 17-171667-5746CONICET DigitalCONICETenghttps://www.biologia.org.ar/eventos/iv-reunion-conjunta-de-sociedades-de-biologia-de-la-republica-argentina/info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v45nSuppl.3/44000/pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:46Zoai:ri.conicet.gov.ar:11336/231401instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:46.485CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Identification of potential proteins involved in angiogenesis associated with cervical cancer using proteomics and bioinformatics approaches
title Identification of potential proteins involved in angiogenesis associated with cervical cancer using proteomics and bioinformatics approaches
spellingShingle Identification of potential proteins involved in angiogenesis associated with cervical cancer using proteomics and bioinformatics approaches
Valero, Victoria
CERVICAL CANCER
ENDOTHELIAL CELLS
ANGIOGENESIS
PROTEOME
title_short Identification of potential proteins involved in angiogenesis associated with cervical cancer using proteomics and bioinformatics approaches
title_full Identification of potential proteins involved in angiogenesis associated with cervical cancer using proteomics and bioinformatics approaches
title_fullStr Identification of potential proteins involved in angiogenesis associated with cervical cancer using proteomics and bioinformatics approaches
title_full_unstemmed Identification of potential proteins involved in angiogenesis associated with cervical cancer using proteomics and bioinformatics approaches
title_sort Identification of potential proteins involved in angiogenesis associated with cervical cancer using proteomics and bioinformatics approaches
dc.creator.none.fl_str_mv Valero, Victoria
Homann, Luana Micaela
Carriere, Pedro Matias
Novoa Díaz, María Belén
Gentili, Claudia Rosana
Calvo, Natalia Graciela
author Valero, Victoria
author_facet Valero, Victoria
Homann, Luana Micaela
Carriere, Pedro Matias
Novoa Díaz, María Belén
Gentili, Claudia Rosana
Calvo, Natalia Graciela
author_role author
author2 Homann, Luana Micaela
Carriere, Pedro Matias
Novoa Díaz, María Belén
Gentili, Claudia Rosana
Calvo, Natalia Graciela
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Parborell, Maria Fernanda Agustina
dc.subject.none.fl_str_mv CERVICAL CANCER
ENDOTHELIAL CELLS
ANGIOGENESIS
PROTEOME
topic CERVICAL CANCER
ENDOTHELIAL CELLS
ANGIOGENESIS
PROTEOME
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Angiogenesis is the growth of blood vessels from the existing vasculature and is essential in the progression of cervical cancer (CC), the fourth most common tumor in women worldwide. This process studies in search of potential biomarkers and therapeutic targets since the endothelial cells that form the abnormal tumor vasculature are characterized by changes at the protein level when are regulated by tumor and microenvironmental factors. The objective of this work was to identify potential proteins involved in the response of endothelial cells to soluble factors released by tumor cells derived from CC, using proteomics and bioinformatics approaches. We previously observed that treatment with conditioned media from CC HeLa cells (TCMs) for 24 h increases the number of endothelial HMEC-1 cells. In this work, the proteome response of HMEC-1 cells was studied underthese experimental conditions, performing a Label-Free quantitative (LFQ) mass spectrometry (MS) at the CEQUIBIEM Proteomics Center. Proteins were identified and quantified with the Proteome Discoverer software and the Uniprot database. Also, a more in-depth statistical study was performed using the Perseus software. Proteomic analysis revealed 26 proteins with increased expression levels in endothelial cells treated with TCM (P ≤ 0.05). Then, to evaluate the biological characteristics of these proteins, they were classified using the PANTHER analysis tool, according to their molecular function and biological processes. As a result of this study, catalytic activity was the most represented molecular function (11/26), followed by binding (4/26). Respect to biological processes, proteins were mainly classified into cellular processes (12/26) and energy metabolism(11/26). This analysis suggests that factors released by tumor cells mainly increase the expression of proteins involved in metabolic processes in endothelial cells. Within these proteins, the probable ATP-dependent RNA helicase DDX47 showed the greatest magnitude of change (> 2). DDX47is related to rRNA processing and ribosome biogenesis, which are processes associated with cell proliferation and cancer progression. Furthermore, ribosomal activity is also a critical regulator of metabolism. These results highlight the use of Label-Free spectrometry and bioinformatics approaches in an initial phase of discovery of potential proteins involved in cancer and suggest the potential role of DDX47 in angiogenesis associated with CC.
Fil: Valero, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Homann, Luana Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Carriere, Pedro Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Novoa Díaz, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Gentili, Claudia Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Calvo, Natalia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
IV Reunión Conjunta de Sociedades de Biología de la República Argentina
Argentina
Sociedad Argentina de Biología
description Angiogenesis is the growth of blood vessels from the existing vasculature and is essential in the progression of cervical cancer (CC), the fourth most common tumor in women worldwide. This process studies in search of potential biomarkers and therapeutic targets since the endothelial cells that form the abnormal tumor vasculature are characterized by changes at the protein level when are regulated by tumor and microenvironmental factors. The objective of this work was to identify potential proteins involved in the response of endothelial cells to soluble factors released by tumor cells derived from CC, using proteomics and bioinformatics approaches. We previously observed that treatment with conditioned media from CC HeLa cells (TCMs) for 24 h increases the number of endothelial HMEC-1 cells. In this work, the proteome response of HMEC-1 cells was studied underthese experimental conditions, performing a Label-Free quantitative (LFQ) mass spectrometry (MS) at the CEQUIBIEM Proteomics Center. Proteins were identified and quantified with the Proteome Discoverer software and the Uniprot database. Also, a more in-depth statistical study was performed using the Perseus software. Proteomic analysis revealed 26 proteins with increased expression levels in endothelial cells treated with TCM (P ≤ 0.05). Then, to evaluate the biological characteristics of these proteins, they were classified using the PANTHER analysis tool, according to their molecular function and biological processes. As a result of this study, catalytic activity was the most represented molecular function (11/26), followed by binding (4/26). Respect to biological processes, proteins were mainly classified into cellular processes (12/26) and energy metabolism(11/26). This analysis suggests that factors released by tumor cells mainly increase the expression of proteins involved in metabolic processes in endothelial cells. Within these proteins, the probable ATP-dependent RNA helicase DDX47 showed the greatest magnitude of change (> 2). DDX47is related to rRNA processing and ribosome biogenesis, which are processes associated with cell proliferation and cancer progression. Furthermore, ribosomal activity is also a critical regulator of metabolism. These results highlight the use of Label-Free spectrometry and bioinformatics approaches in an initial phase of discovery of potential proteins involved in cancer and suggest the potential role of DDX47 in angiogenesis associated with CC.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
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Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/231401
Identification of potential proteins involved in angiogenesis associated with cervical cancer using proteomics and bioinformatics approaches; IV Reunión Conjunta de Sociedades de Biología de la República Argentina; Argentina; 2020; 17-17
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/231401
identifier_str_mv Identification of potential proteins involved in angiogenesis associated with cervical cancer using proteomics and bioinformatics approaches; IV Reunión Conjunta de Sociedades de Biología de la República Argentina; Argentina; 2020; 17-17
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.biologia.org.ar/eventos/iv-reunion-conjunta-de-sociedades-de-biologia-de-la-republica-argentina/
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
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