Regulation of Dopamine Release by CASK-β Modulates Locomotor Initiation in Drosophila melanogaster

Autores
Slawson, Justin B; Kuklin, Elena A; Mukherjee, Konark; Pírez, Nicolas; Donelson, Nathan C; Griffith, Leslie C
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
CASK is an evolutionarily conserved scaffolding protein that has roles in many cell types. In Drosophila, loss of the entire CASK gene or just the CASK- transcript causes a complex set of adult locomotor defects. In this study, we show that the motor initiation component of this phenotype is due to loss of CASK- in dopaminergic neurons and can be specifically rescued by expression of CASK- within this subset of neurons. Functional imaging demonstrates that mutation of CASK- disrupts coupling of neuronal activity to vesicle fusion. Consistent with this, locomotor initiation can be rescued by artificially driving activity in dopaminergic neurons. The molecular mechanism underlying this role of CASK- in dopaminergic neurons involves interaction with Hsc70-4, a molecular chaperone previously shown to regulate calcium-dependent vesicle fusion. These data suggest that there is a novel CASK- -dependent regulatory complex in dopaminergic neurons that serves to link activity and neurotransmitter release.
Fil: Slawson, Justin B. Brandeis University; Estados Unidos
Fil: Kuklin, Elena A. Brandeis University; Estados Unidos
Fil: Mukherjee, Konark. Brandeis University; Estados Unidos
Fil: Pírez, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina. Brandeis University; Estados Unidos
Fil: Donelson, Nathan C. Brandeis University; Estados Unidos
Fil: Griffith, Leslie C. Brandeis University; Estados Unidos
Materia
Drosophila melanogaster
neurotransmitter release
CASK
dopamine
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8344
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/8344
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Regulation of Dopamine Release by CASK-β Modulates Locomotor Initiation in Drosophila melanogasterSlawson, Justin BKuklin, Elena AMukherjee, KonarkPírez, NicolasDonelson, Nathan CGriffith, Leslie CDrosophila melanogasterneurotransmitter releaseCASKdopaminehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1CASK is an evolutionarily conserved scaffolding protein that has roles in many cell types. In Drosophila, loss of the entire CASK gene or just the CASK- transcript causes a complex set of adult locomotor defects. In this study, we show that the motor initiation component of this phenotype is due to loss of CASK- in dopaminergic neurons and can be specifically rescued by expression of CASK- within this subset of neurons. Functional imaging demonstrates that mutation of CASK- disrupts coupling of neuronal activity to vesicle fusion. Consistent with this, locomotor initiation can be rescued by artificially driving activity in dopaminergic neurons. The molecular mechanism underlying this role of CASK- in dopaminergic neurons involves interaction with Hsc70-4, a molecular chaperone previously shown to regulate calcium-dependent vesicle fusion. These data suggest that there is a novel CASK- -dependent regulatory complex in dopaminergic neurons that serves to link activity and neurotransmitter release.Fil: Slawson, Justin B. Brandeis University; Estados UnidosFil: Kuklin, Elena A. Brandeis University; Estados UnidosFil: Mukherjee, Konark. Brandeis University; Estados UnidosFil: Pírez, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina. Brandeis University; Estados UnidosFil: Donelson, Nathan C. Brandeis University; Estados UnidosFil: Griffith, Leslie C. Brandeis University; Estados UnidosFrontiers2014-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8344Slawson, Justin B; Kuklin, Elena A; Mukherjee, Konark; Pírez, Nicolas; Donelson, Nathan C; et al.; Regulation of Dopamine Release by CASK-β Modulates Locomotor Initiation in Drosophila melanogaster; Frontiers; Frontiers in Behavioral Neuroscience; 8; 394; 11-2014; 1-111662-5153enginfo:eu-repo/semantics/altIdentifier/url/http://journal.frontiersin.org/article/10.3389/fnbeh.2014.00394/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fnbeh.2014.00394info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:18:57Zoai:ri.conicet.gov.ar:11336/8344instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:18:58.39CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Regulation of Dopamine Release by CASK-β Modulates Locomotor Initiation in Drosophila melanogaster
title Regulation of Dopamine Release by CASK-β Modulates Locomotor Initiation in Drosophila melanogaster
spellingShingle Regulation of Dopamine Release by CASK-β Modulates Locomotor Initiation in Drosophila melanogaster
Slawson, Justin B
Drosophila melanogaster
neurotransmitter release
CASK
dopamine
title_short Regulation of Dopamine Release by CASK-β Modulates Locomotor Initiation in Drosophila melanogaster
title_full Regulation of Dopamine Release by CASK-β Modulates Locomotor Initiation in Drosophila melanogaster
title_fullStr Regulation of Dopamine Release by CASK-β Modulates Locomotor Initiation in Drosophila melanogaster
title_full_unstemmed Regulation of Dopamine Release by CASK-β Modulates Locomotor Initiation in Drosophila melanogaster
title_sort Regulation of Dopamine Release by CASK-β Modulates Locomotor Initiation in Drosophila melanogaster
dc.creator.none.fl_str_mv Slawson, Justin B
Kuklin, Elena A
Mukherjee, Konark
Pírez, Nicolas
Donelson, Nathan C
Griffith, Leslie C
author Slawson, Justin B
author_facet Slawson, Justin B
Kuklin, Elena A
Mukherjee, Konark
Pírez, Nicolas
Donelson, Nathan C
Griffith, Leslie C
author_role author
author2 Kuklin, Elena A
Mukherjee, Konark
Pírez, Nicolas
Donelson, Nathan C
Griffith, Leslie C
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Drosophila melanogaster
neurotransmitter release
CASK
dopamine
topic Drosophila melanogaster
neurotransmitter release
CASK
dopamine
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv CASK is an evolutionarily conserved scaffolding protein that has roles in many cell types. In Drosophila, loss of the entire CASK gene or just the CASK- transcript causes a complex set of adult locomotor defects. In this study, we show that the motor initiation component of this phenotype is due to loss of CASK- in dopaminergic neurons and can be specifically rescued by expression of CASK- within this subset of neurons. Functional imaging demonstrates that mutation of CASK- disrupts coupling of neuronal activity to vesicle fusion. Consistent with this, locomotor initiation can be rescued by artificially driving activity in dopaminergic neurons. The molecular mechanism underlying this role of CASK- in dopaminergic neurons involves interaction with Hsc70-4, a molecular chaperone previously shown to regulate calcium-dependent vesicle fusion. These data suggest that there is a novel CASK- -dependent regulatory complex in dopaminergic neurons that serves to link activity and neurotransmitter release.
Fil: Slawson, Justin B. Brandeis University; Estados Unidos
Fil: Kuklin, Elena A. Brandeis University; Estados Unidos
Fil: Mukherjee, Konark. Brandeis University; Estados Unidos
Fil: Pírez, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina. Brandeis University; Estados Unidos
Fil: Donelson, Nathan C. Brandeis University; Estados Unidos
Fil: Griffith, Leslie C. Brandeis University; Estados Unidos
description CASK is an evolutionarily conserved scaffolding protein that has roles in many cell types. In Drosophila, loss of the entire CASK gene or just the CASK- transcript causes a complex set of adult locomotor defects. In this study, we show that the motor initiation component of this phenotype is due to loss of CASK- in dopaminergic neurons and can be specifically rescued by expression of CASK- within this subset of neurons. Functional imaging demonstrates that mutation of CASK- disrupts coupling of neuronal activity to vesicle fusion. Consistent with this, locomotor initiation can be rescued by artificially driving activity in dopaminergic neurons. The molecular mechanism underlying this role of CASK- in dopaminergic neurons involves interaction with Hsc70-4, a molecular chaperone previously shown to regulate calcium-dependent vesicle fusion. These data suggest that there is a novel CASK- -dependent regulatory complex in dopaminergic neurons that serves to link activity and neurotransmitter release.
publishDate 2014
dc.date.none.fl_str_mv 2014-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8344
Slawson, Justin B; Kuklin, Elena A; Mukherjee, Konark; Pírez, Nicolas; Donelson, Nathan C; et al.; Regulation of Dopamine Release by CASK-β Modulates Locomotor Initiation in Drosophila melanogaster; Frontiers; Frontiers in Behavioral Neuroscience; 8; 394; 11-2014; 1-11
1662-5153
url http://hdl.handle.net/11336/8344
identifier_str_mv Slawson, Justin B; Kuklin, Elena A; Mukherjee, Konark; Pírez, Nicolas; Donelson, Nathan C; et al.; Regulation of Dopamine Release by CASK-β Modulates Locomotor Initiation in Drosophila melanogaster; Frontiers; Frontiers in Behavioral Neuroscience; 8; 394; 11-2014; 1-11
1662-5153
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://journal.frontiersin.org/article/10.3389/fnbeh.2014.00394/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fnbeh.2014.00394
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers
publisher.none.fl_str_mv Frontiers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842981032019623936
score 12.48226

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