Micro-Environmental Variation in Soil Microbial Biodiversity in Forest Frontier Ecosystems—Implications for Sustainability Assessments

Autores
Alvarez, Valeria Esther; El Mujtar, Verónica Andrea; Falcão Salles, Joana; Jia, Xiu; Castán, Elisa; Cardozo, Andrea Gabriela; Tittonell, Pablo
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Soil health indicators based on microbial biodiversity are increasingly used in agricultural sustainability assessments. However, little is known about how microbial communities vary within micro-environmental gradients across different land uses, which is crucial for designing field sampling and monitoring protocols. Our objective was to assess how soil microbial communities changed with soil depth and spatial distance across land-use types. We sampled soils in four spatial distances (within 0.1–70 m) and four depths (within 0–40 cm) in forests, grasslands, and horticultural lands, and combined 16S rRNA gene sequencing, DNA quantification and soil chemical characterization to explore micro-environmental variation in microbial biomass, α-β-diversity, and communities’ assembly processes. Depth and spatial distance had differential effects on microbial biodiversity within different land uses. Microbial biomass was most sensitive to depth, α-diversity to spatial distance, and β-diversity to both depth and spatial distance. Deterministic processes dominate microbial communities’ assembly along depth in all land uses, which is a promising result for developing soil quality indicators based on microbial biodiversity. Overall, our results suggest that collecting soil samples separated by at least 12 m is adequate to capture biodiversity changes across land uses. However, collecting randomly within the first 10 cm is recommended for native forests, while systematic sampling within the first 20 cm is advised for grasslands and horticultural lands. Our findings underscore the need for land use-specific sampling frameworks in soil life-based sustainability assessments for meaningful regional comparisons.
Fil: Alvarez, Valeria Esther. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: El Mujtar, Verónica Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Falcão Salles, Joana. University of Groningen; Países Bajos
Fil: Jia, Xiu. University of Groningen; Países Bajos
Fil: Castán, Elisa. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Cardozo, Andrea Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Agencia de Extensión Rural El Bolsón; Argentina
Fil: Tittonell, Pablo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina. University of Groningen; Países Bajos. Université Montpellier II; Francia
Materia
EVOLUTIONARY PROCESSES
MICROBIAL ECOLOGY
PATAGONIA
SOIL BIOTA
SOIL DEPTH
SPATIAL AUTOCORRELATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/266970
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Micro-Environmental Variation in Soil Microbial Biodiversity in Forest Frontier Ecosystems—Implications for Sustainability AssessmentsAlvarez, Valeria EstherEl Mujtar, Verónica AndreaFalcão Salles, JoanaJia, XiuCastán, ElisaCardozo, Andrea GabrielaTittonell, PabloEVOLUTIONARY PROCESSESMICROBIAL ECOLOGYPATAGONIASOIL BIOTASOIL DEPTHSPATIAL AUTOCORRELATIONhttps://purl.org/becyt/ford/1.7https://purl.org/becyt/ford/1Soil health indicators based on microbial biodiversity are increasingly used in agricultural sustainability assessments. However, little is known about how microbial communities vary within micro-environmental gradients across different land uses, which is crucial for designing field sampling and monitoring protocols. Our objective was to assess how soil microbial communities changed with soil depth and spatial distance across land-use types. We sampled soils in four spatial distances (within 0.1–70 m) and four depths (within 0–40 cm) in forests, grasslands, and horticultural lands, and combined 16S rRNA gene sequencing, DNA quantification and soil chemical characterization to explore micro-environmental variation in microbial biomass, α-β-diversity, and communities’ assembly processes. Depth and spatial distance had differential effects on microbial biodiversity within different land uses. Microbial biomass was most sensitive to depth, α-diversity to spatial distance, and β-diversity to both depth and spatial distance. Deterministic processes dominate microbial communities’ assembly along depth in all land uses, which is a promising result for developing soil quality indicators based on microbial biodiversity. Overall, our results suggest that collecting soil samples separated by at least 12 m is adequate to capture biodiversity changes across land uses. However, collecting randomly within the first 10 cm is recommended for native forests, while systematic sampling within the first 20 cm is advised for grasslands and horticultural lands. Our findings underscore the need for land use-specific sampling frameworks in soil life-based sustainability assessments for meaningful regional comparisons.Fil: Alvarez, Valeria Esther. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: El Mujtar, Verónica Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Falcão Salles, Joana. University of Groningen; Países BajosFil: Jia, Xiu. University of Groningen; Países BajosFil: Castán, Elisa. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Cardozo, Andrea Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Agencia de Extensión Rural El Bolsón; ArgentinaFil: Tittonell, Pablo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina. University of Groningen; Países Bajos. Université Montpellier II; FranciaMultidisciplinary Digital Publishing Institute2024-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/266970Alvarez, Valeria Esther; El Mujtar, Verónica Andrea; Falcão Salles, Joana; Jia, Xiu; Castán, Elisa; et al.; Micro-Environmental Variation in Soil Microbial Biodiversity in Forest Frontier Ecosystems—Implications for Sustainability Assessments; Multidisciplinary Digital Publishing Institute; Sustainability; 16; 3; 2-2024; 1-262071-1050CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2071-1050/16/3/1236info:eu-repo/semantics/altIdentifier/doi/10.3390/su16031236info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:34:03Zoai:ri.conicet.gov.ar:11336/266970instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:34:03.586CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Micro-Environmental Variation in Soil Microbial Biodiversity in Forest Frontier Ecosystems—Implications for Sustainability Assessments
title Micro-Environmental Variation in Soil Microbial Biodiversity in Forest Frontier Ecosystems—Implications for Sustainability Assessments
spellingShingle Micro-Environmental Variation in Soil Microbial Biodiversity in Forest Frontier Ecosystems—Implications for Sustainability Assessments
Alvarez, Valeria Esther
EVOLUTIONARY PROCESSES
MICROBIAL ECOLOGY
PATAGONIA
SOIL BIOTA
SOIL DEPTH
SPATIAL AUTOCORRELATION
title_short Micro-Environmental Variation in Soil Microbial Biodiversity in Forest Frontier Ecosystems—Implications for Sustainability Assessments
title_full Micro-Environmental Variation in Soil Microbial Biodiversity in Forest Frontier Ecosystems—Implications for Sustainability Assessments
title_fullStr Micro-Environmental Variation in Soil Microbial Biodiversity in Forest Frontier Ecosystems—Implications for Sustainability Assessments
title_full_unstemmed Micro-Environmental Variation in Soil Microbial Biodiversity in Forest Frontier Ecosystems—Implications for Sustainability Assessments
title_sort Micro-Environmental Variation in Soil Microbial Biodiversity in Forest Frontier Ecosystems—Implications for Sustainability Assessments
dc.creator.none.fl_str_mv Alvarez, Valeria Esther
El Mujtar, Verónica Andrea
Falcão Salles, Joana
Jia, Xiu
Castán, Elisa
Cardozo, Andrea Gabriela
Tittonell, Pablo
author Alvarez, Valeria Esther
author_facet Alvarez, Valeria Esther
El Mujtar, Verónica Andrea
Falcão Salles, Joana
Jia, Xiu
Castán, Elisa
Cardozo, Andrea Gabriela
Tittonell, Pablo
author_role author
author2 El Mujtar, Verónica Andrea
Falcão Salles, Joana
Jia, Xiu
Castán, Elisa
Cardozo, Andrea Gabriela
Tittonell, Pablo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv EVOLUTIONARY PROCESSES
MICROBIAL ECOLOGY
PATAGONIA
SOIL BIOTA
SOIL DEPTH
SPATIAL AUTOCORRELATION
topic EVOLUTIONARY PROCESSES
MICROBIAL ECOLOGY
PATAGONIA
SOIL BIOTA
SOIL DEPTH
SPATIAL AUTOCORRELATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.7
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Soil health indicators based on microbial biodiversity are increasingly used in agricultural sustainability assessments. However, little is known about how microbial communities vary within micro-environmental gradients across different land uses, which is crucial for designing field sampling and monitoring protocols. Our objective was to assess how soil microbial communities changed with soil depth and spatial distance across land-use types. We sampled soils in four spatial distances (within 0.1–70 m) and four depths (within 0–40 cm) in forests, grasslands, and horticultural lands, and combined 16S rRNA gene sequencing, DNA quantification and soil chemical characterization to explore micro-environmental variation in microbial biomass, α-β-diversity, and communities’ assembly processes. Depth and spatial distance had differential effects on microbial biodiversity within different land uses. Microbial biomass was most sensitive to depth, α-diversity to spatial distance, and β-diversity to both depth and spatial distance. Deterministic processes dominate microbial communities’ assembly along depth in all land uses, which is a promising result for developing soil quality indicators based on microbial biodiversity. Overall, our results suggest that collecting soil samples separated by at least 12 m is adequate to capture biodiversity changes across land uses. However, collecting randomly within the first 10 cm is recommended for native forests, while systematic sampling within the first 20 cm is advised for grasslands and horticultural lands. Our findings underscore the need for land use-specific sampling frameworks in soil life-based sustainability assessments for meaningful regional comparisons.
Fil: Alvarez, Valeria Esther. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: El Mujtar, Verónica Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Falcão Salles, Joana. University of Groningen; Países Bajos
Fil: Jia, Xiu. University of Groningen; Países Bajos
Fil: Castán, Elisa. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Cardozo, Andrea Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Agencia de Extensión Rural El Bolsón; Argentina
Fil: Tittonell, Pablo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina. University of Groningen; Países Bajos. Université Montpellier II; Francia
description Soil health indicators based on microbial biodiversity are increasingly used in agricultural sustainability assessments. However, little is known about how microbial communities vary within micro-environmental gradients across different land uses, which is crucial for designing field sampling and monitoring protocols. Our objective was to assess how soil microbial communities changed with soil depth and spatial distance across land-use types. We sampled soils in four spatial distances (within 0.1–70 m) and four depths (within 0–40 cm) in forests, grasslands, and horticultural lands, and combined 16S rRNA gene sequencing, DNA quantification and soil chemical characterization to explore micro-environmental variation in microbial biomass, α-β-diversity, and communities’ assembly processes. Depth and spatial distance had differential effects on microbial biodiversity within different land uses. Microbial biomass was most sensitive to depth, α-diversity to spatial distance, and β-diversity to both depth and spatial distance. Deterministic processes dominate microbial communities’ assembly along depth in all land uses, which is a promising result for developing soil quality indicators based on microbial biodiversity. Overall, our results suggest that collecting soil samples separated by at least 12 m is adequate to capture biodiversity changes across land uses. However, collecting randomly within the first 10 cm is recommended for native forests, while systematic sampling within the first 20 cm is advised for grasslands and horticultural lands. Our findings underscore the need for land use-specific sampling frameworks in soil life-based sustainability assessments for meaningful regional comparisons.
publishDate 2024
dc.date.none.fl_str_mv 2024-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/266970
Alvarez, Valeria Esther; El Mujtar, Verónica Andrea; Falcão Salles, Joana; Jia, Xiu; Castán, Elisa; et al.; Micro-Environmental Variation in Soil Microbial Biodiversity in Forest Frontier Ecosystems—Implications for Sustainability Assessments; Multidisciplinary Digital Publishing Institute; Sustainability; 16; 3; 2-2024; 1-26
2071-1050
CONICET Digital
CONICET
url http://hdl.handle.net/11336/266970
identifier_str_mv Alvarez, Valeria Esther; El Mujtar, Verónica Andrea; Falcão Salles, Joana; Jia, Xiu; Castán, Elisa; et al.; Micro-Environmental Variation in Soil Microbial Biodiversity in Forest Frontier Ecosystems—Implications for Sustainability Assessments; Multidisciplinary Digital Publishing Institute; Sustainability; 16; 3; 2-2024; 1-26
2071-1050
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2071-1050/16/3/1236
info:eu-repo/semantics/altIdentifier/doi/10.3390/su16031236
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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